Interspecies Hydrogen Transfer Research Articles

Enhanced degradation of phenolic compounds in coal gasification wastewater by an iron‑carbon micro-electric field coupled with anaerobic co-digestion

Coal gasification wastewater contains many refractory and toxic pollutants, especially high concentrations of total phenols, which are difficult to degrade by microorganisms. The aim of our study is to explore the anaerobically enhanced degradation of coal gasification wastewater by an iron‑carbon micro-electric field coupled with anaerobic co-digestion. The optimal ratio of activated carbon to iron and the optimal dosage of co-substrate (glucose = 1500 mg/L) were investigated by batch tests. In the long-term operation of the iron‑carbon reactor, 1500 mg/L glucose was added into the influent, and carbon and iron in a ratio of 2:1 were added to the anaerobic sludge. The average effluent COD and total phenols concentrations were kept at approximately 455 and 56.3 mg/L, respectively, and removal rates of both reached 90% after treatment with the iron‑carbon micro-electric field coupled with anaerobic co-digestion in the iron‑carbon reactor. Moreover, compared with the control reactor, the methane production from the iron‑carbon reactor increased to 200 mL/day, with an increase in the methane production rate by 90%. Microbial community analysis indicated that hydrogenotrophic methanogens were enriched, and syntrophic metabolism via interspecies hydrogen transfer was enhanced. Direct interspecies electron transfer might occur between the potential electroactive bacteria Clostridium, Bacteroidetes, and Anaerolinea and the methanogens Methanosaeta, Methanobacterialies, and Methanobacterium for syntrophic metabolism through the iron‑carbon process coupled with anaerobic co-digestion.

New insights into the mechanisms underlying biochar-assisted sustained high-efficient co-digestion: Reducing thermodynamic constraints and enhancing extracellular electron transfer flux

To clarify the roles of biochar in the anaerobic co-digestion of waste activated sludge (WAS) and food waste (FW), batch tests were conducted coupled with thermodynamics, extracellular electron transfer flux and microbial community analysis. Compared with the control group, biochar significantly facilitated the co-digestion at three periods, but its sustainable facilitation was mainly in the syntrophic methanogenesis of volatile fatty acids (VFAs). The thermodynamic analysis confirmed that biochar could alleviate limitations imposed by high hydrogen partial pressure during interspecies hydrogen transfer (IHT), the thermodynamic windows was expanded 137% and 92% in the syntrophic methanogenesis of acetate and propionate, respectively. Meanwhile, due to the redox capacity of biochar (4.85 and 0.35 μmol e−/g biochar), the equivalent current of direct interspecies electron transfer (DIET) flux for syntrophic methanogenesis of acetate and propionate obtained were 1.0 × 10−4 A and 0.9 × 10−4 A, which were 108 times than that of IHT. It should be noticed that the functional microorganisms like Methanosarcina which could participate DIET were only enriched on the surface of biochar, the dominant Methanothermobacter in suspended sludge probably indicate IHT was still the main pathway for syntrophic methanogenesis. Nevertheless, the DIET triggered by the redox-active moieties on the surface of biochar and the enhanced IHT by alleviating thermodynamic restrictions, promoted the syntrophic methanogenesis synergistically.

Disentangling the syntrophic electron transfer mechanisms of Candidatus geobacter eutrophica through electrochemical stimulation and machine learning

Interspecies hydrogen transfer (IHT) and direct interspecies electron transfer (DIET) are two syntrophy models for methanogenesis. Their relative importance in methanogenic environments is still unclear. Our recent discovery of a novel species Candidatus Geobacter eutrophica with the genetic potential of IHT and DIET may serve as a model species to address this knowledge gap. To experimentally demonstrate its DIET ability, we performed electrochemical enrichment of Ca. G. eutrophica-dominating communities under 0 and 0.4 V vs. Ag/AgCl based on the presumption that DIET and extracellular electron transfer (EET) share similar metabolic pathways. After three batches of enrichment, Geobacter OTU650, which was phylogenetically close to Ca. G. eutrophica, was outcompeted in the control but remained abundant and active under electrochemical stimulation, indicating Ca. G. eutrophica’s EET ability. The high-quality draft genome further showed high phylogenomic similarity with Ca. G. eutrophica, and the genes encoding outer membrane cytochromes and enzymes for hydrogen metabolism were actively expressed. A Bayesian network was trained with the genes encoding enzymes for alcohol metabolism, hydrogen metabolism, EET, and methanogenesis from dominant fermentative bacteria, Geobacter, and Methanobacterium. Methane production could not be accurately predicted when the genes for IHT were in silico knocked out, inferring its more important role in methanogenesis. The genomics-enabled machine learning modeling approach can provide predictive insights into the importance of IHT and DIET.

Using Structural Equation Modeling to Understand Interactions Between Bacterial and Archaeal Populations and Volatile Fatty Acid Proportions in the Rumen.

Microbial syntrophy (obligate metabolic mutualism) is the hallmark of energy-constrained anaerobic microbial ecosystems. For example, methanogenic archaea and fermenting bacteria coexist by interspecies hydrogen transfer in the complex microbial ecosystem in the foregut of ruminants; however, these synergistic interactions between different microbes in the rumen are seldom investigated. We hypothesized that certain bacteria and archaea interact and form specific microbial cohorts in the rumen. To this end, we examined the total (DNA-based) and potentially metabolically active (cDNA-based) bacterial and archaeal communities in rumen samples of dairy cows collected at different times in a 24 h period. Notably, we found the presence of distinct bacterial and archaeal networks showing potential metabolic interactions that were correlated with molar proportions of specific volatile fatty acids (VFAs). We employed hypothesis-driven structural equation modeling to test the significance of and to quantify the extent of these relationships between bacteria-archaea-VFAs in the rumen. Furthermore, we demonstrated that these distinct microbial networks were host-specific and differed between cows indicating a natural variation in specific microbial networks in the rumen of dairy cows. This study provides new insights on potential microbial metabolic interactions in anoxic environments that have broader applications in methane mitigation, energy conservation, and agricultural production.

Self-fluidized GAC-amended UASB reactor for enhanced methane production

Granular activated carbon (GAC) has been reported to benefit anaerobic digestion systems by stimulating direct interspecies electron transfer (DIET) as an alternative to interspecies hydrogen transfer (IHT). Conventional up-flow anaerobic sludge blanket (UASB) reactors typically have GAC settled at the bottom of the reactor, limiting the contact between biomass and GAC. This study demonstrated a modified UASB reactor that fluidizes GAC by encaging GAC in plastic carriers. Enhanced performance was achieved with respect to methane production and COD removal at an organic loading rate of 1500 g COD/m3/d under the temperature of 20 °C using the self-fluidized GAC configuration; with the methanation rate increased from 0.33 ± 0.08 g CH4-COD/g influent COD in the non-GAC reactor, to 0.66 ± 0.02 g CH4-COD/g influent COD in the GAC-only reactor, and further increased to 0.77 ± 0.02 g CH4-COD/g influent COD in the self-fluidized GAC reactor. The concentrations of medium-chain acyl-homoserine lactones increased in the GAC-amended reactors, potentially associated with the promotion of syntrophic interactions between bacteria and archaea. Batch tests were performed for syntrophic propionate degradation under hydrogen-inhibition conditions, and showed that microbial consortium enriched in both GAC amended reactors can overcome hydrogen inhibition. Further microbial community analysis unveiled a variation in spatial distribution in the GAC enriched microbial consortium (including DIET indicator microorganisms such as Geobacter and Methanosarcina) based on the location of GAC in the reactors. This study demonstrated an improved GAC amendment strategy in UASB reactors to facilitate biomethane recovery from wastewater anaerobic digestion.

Dual roles of zero-valent iron in dry anaerobic digestion: Enhancing interspecies hydrogen transfer and direct interspecies electron transfer

Although commonly viewed as a promising method, dry anaerobic digestion is not been widely applied to dispose of food wastes, especially in developing countries because of its insufficiency in handling with lower mass transfer and high acidic accumulation of the system. Zero valent iron (ZVI) has been found to demonstrate superior performance such as enhancing methane production. However, up to date, the mechanism of ZVI remains unclear. In this study, adding 5 g/L ZVI could improve interspecies hydrogen transfer (IHT) to enhance the dry anaerobic digestion of food wastes, but was unable to resist the shocks of high organic loading. With increasing ZVI dosage to 10 g/L, the performances of digestion systems were improved to maintain the systems stable. With 10 g/L of ZVI addition, electron transfer capacity of the sludge increased by 5.4 folds, and electroactive proteins of sludge increased by 2.3 folds. Microbial community analysis also indicated that the relative abundances of Methanothrix and Methanosarcina performing direct interspecies electron transfer were enriched to 67.5% and 27.2% with 10 g/L ZVI addition, respectively. These results suggested that direct interspecies electron transfer could be established with a proper dosage of ZVI that served as a conductive material to connect electron exchange among microorganisms. Thus, ZVI played a dual role including improving interspecies hydrogen transfer and promoting direct interspecies electron transfer to keep the systems efficient to treat high-solid food wastes.

Direct interspecies electron transfer in anaerobic digestion: Research and technological application

Anaerobic digestion (AD), an economical and eco-friendly strategy for renewable energy recovery, has been widely investigated and applied in the stabilization of various organic wastes. However, two major factors involved in the slow fermentation rate and low methane conversion efficiency still limit its large-scale applications around the world. Methane formation via conventional methanogenic pathway in AD undergoes the three stages: Hydrolysis/acidogenesis, acetogenesis and methanogenesis. The structure of some saccharide- and protein-like organic compounds contained in complex organic waste is relatively complex and stable, limiting the rate of converting them into amino acids, fatty acids and alcohols by fermentative bacteria. Therefore, the hydrolysis/acidogenesis is widely recognized as a rate-limiting step of AD in practical applications. On the other hand, the obligate anaerobic methanogens can only utilize methanol, methylamine, formate, acetate and hydrogen/carbon dioxide as substrates. Methane formation with amino acids, fatty acids and alcohols as substrates requires the essential conversion of them into acetate and hydrogen/carbon dioxide by acetogenic bacteria, and then depends on acetate- and hydrogen-utilizing methanogens to metabolize acetate and hydrogen/carbon dioxide into methane, respectively. This working mode of syntrophic metabolism of organic compounds into methane between acetogenic bacteria and methanogens that depends on hydrogen as an interspecies electron carrier has been widely described as Interspecies Hydrogen Transfer (IHT). Formate may be also served as a substitute for hydrogen as an interspecies electron carrier (Interspecies Formate Transfer, IFT). Syntrophic metabolism of organic compounds is strictly limited by the hydrogen partial pressure, since the oxidation of organic compounds into acetate with H+ as an electron acceptor is not feasible in thermodynamics ( E H + / H 2 = −0.414 V, pH 7). Syntrophic metabolism via IHT functions well as long as the hydrogen-utilizing methanogens continuously maintain the hydrogen partial pressure low enough (

Novel strategy for relieving acid accumulation by enriching syntrophic associations of syntrophic fatty acid-oxidation bacteria and H2/formate-scavenging methanogens in anaerobic digestion

Aiming at relieving acid accumulation in anaerobic digestion (AD), syntrophic associations of syntrophic fatty acid-oxidation bacteria and H2/formate-scavenging methanogens were enriched by feeding propionate, butyrate and formate in an up-flow anaerobic sludge blanket (UASB) reactor. Results showed that methane yield increased by 50% with increasing formate concentration (0–2000 mg COD/L). In addition, the abundance and quantity of SFOB (Syntrophobacter, Smithella and Syntrophomonas) and H2/formate-scavenging methanogens (Methanobacteriales and Methanomicrobiales) were increased after microbial acclimation. The enriched syntrophic associations showed higher propionate and butyrate removal efficiencies of 98.48 ± 1.14% and 99.71 ± 0.71%, respectively. Furthermore, encoding genes of formate dehydrogenase and hydrogenases presented higher abundances after microbial enrichment, which suggested that the enhancements of interspecies formate transfer and interspecies hydrogen transfer between syntrophic associations benefited volatile fatty acids (VFAs) conversion. This research provided an effective strategy to relieve acid accumulation.

It does not always take two to tango: \u201cSyntrophy\u201d via hydrogen cycling in one bacterial cell

Interspecies hydrogen transfer in anoxic ecosystems is essential for the complete microbial breakdown of organic matter to methane. Acetogenic bacteria are key players in anaerobic food webs and have been considered as prime candidates for hydrogen cycling. We have tested this hypothesis by mutational analysis of the hydrogenase in the model acetogen Acetobacterium woodii. Hydrogenase-deletion mutants no longer grew on H2 + CO2 or organic substrates such as fructose, lactate, or ethanol. Heterotrophic growth could be restored by addition of molecular hydrogen to the culture, indicating that hydrogen is an intermediate in heterotrophic growth. Indeed, hydrogen production from fructose was detected in a stirred-tank reactor. The mutant grew well on organic substrates plus caffeate, an alternative electron acceptor that does not require molecular hydrogen but NADH as reductant. These data are consistent with the notion that molecular hydrogen is produced from organic substrates and then used as reductant for CO2 reduction. Surprisingly, hydrogen cycling in A. woodii is different from the known modes of interspecies or intraspecies hydrogen cycling. Our data are consistent with a novel type of hydrogen cycling that connects an oxidative and reductive metabolic module in one bacterial cell, “intracellular syntrophy.”

Enhancing methanogenesis from anaerobic digestion of propionate with addition of Fe oxides supported on conductive carbon cloth

In this study, a Fe2O3 supported on conductive carbon cloth (FC) was prepared and supplemented into anaerobic digestion reactors to improve propionate degradation. In the FC-supplemented reactors, the cumulative methane production and propionate degradation increased by 15.4% and 19.67% compared with those of the control, respectively. Less methane production with H2/CO2 as the sole substrate in the culture taken from the FC reactors suggested that interspecies hydrogen transfer in the FC reactors was weaker. These results suggested that direct interspecies electron transfer (DIET) was established in the FC reactors to improve the performance. Fe2O3 increased the secretion of electron shuttle components of extracellular polymeric substances to increase electron exchange capacity of biomass of the FC reactors, which further facilitated the DIET. Analysis on microbial communities confirmed that the abundance of microorganisms-related DIET in the FC reactors was higher than that in the control.

Bioturbation effect of fortified Daqu on microbial community and flavor metabolite in Chinese strong-flavor liquor brewing microecosystem.

Traditional spontaneous fermentation in microecosystem with microbial successions and environmental variables results in inconsistent quality of fermented foods. We therefore propose the directional bioturbation for microbiota regulation and metabolites production in food fermentation. Here, we revealed the bioturbation effect of fortified Daqu on microbial community based on taxonomic composition, co-occurrence network, and metabolic potential, using Chinese strong-flavor liquor fermentation as a microecosystem. According to principal coordinate analysis, microbial communities were obviously influenced by the bioturbation of fortified Daqu. More specifically, bioturbation increased the abundances of Caproiciproducens, Clostridium, Aspergillus, Candida, Methanobacterium, and Methanosarcina, while decreased that of Lactobacillus. Meanwhile, higher abundances of most genes that encoding enzymes involved in interspecies hydrogen transfer between hexanoic acid bacteria and methanogens were observed in the bioturbated ecosystem by PICRUSt approach. Additionally, co-occurrence analysis showed that bioturbation increased the diversity and complexity of interspecies interactions in microecosystem, which contributed to higher production of flavor metabolites such as hexanoic acid, ethyl hexanoate, and hexyl hexanoate. These results indicated that the bioturbation of fortified Daqu is feasible for flavor metabolism by interspecies interactions of functional microbiota in liquor fermentation. Taken together, it is of great importance for regulating Chinese liquor and even other foods fermentation by bioturbation.

Quantitative analysis of acetate oxidation in the presence of iron in a thermophilic methanogenic reactor

In natural and engineered anaerobic ecosystems, syntrophic oxidation of short-chain fatty acid is one of the key factors and interspecies electron transfer mechanisms between bacteria and archaea play a pivotal role. Growing evidence suggests that microorganisms have the capability to promote interspecies electrons transfer in a more direct manner. In the current study, three different types of iron oxides were used to thermophilic anaerobic reactors (at 50 °C), quantitative polymerase chain reaction and 16S rRNA sequencing technique were applied to investigate the relation between iron reduction bacteria and syntrophic acetate oxidation bacteria. Experiment results showed that goethite and hematite promoted the methane production and generation rate and partially relieved the negative effects of H2. The syntrophic relation between Syntrophaceticus and Methanobacterium worked with a direct interspecies electron transfer model rather than interspecies hydrogen transfer in goethite and hematite dosed reactors. The gene copies of Syntrophaceticus predominated over that of Geobacter. Theoretical calculations revealed that the rates of iron oxide mediated interspecies electron transfer among syntrophic partners were 105-106 times higher than those attainable via interspecies H2 transfer.

A review of interspecies electron transfer in anaerobic digestion

Anaerobic digestion (AD) is an effective way to recover energy from organic waste. About 70% of the methane emitted into the atmosphere is derived from the degradation of organic matter by microorganisms under anaerobic conditions. Interspecies electron transfer (IET) is the key link of syntrophic methanogenesis, an in-depth understanding of IET during AD contributes to the rational use of energy. IET mainly includes three modes, namely, interspecies hydrogen transfer (IHT), interspecies formate transfer (IFT) and interspecies direct electron transfer (DIET). This review summarized and analyzed the IET patterns in the AD process, and related metabolic mechanisms and existing studies were explained.

Symbiotic magnetic motility.

Microscopy and genomic analyses reveal an intriguing symbiosis between eukaryotic protists and Deltaproteobacteria in anoxic marine sediments that involves division of labour and interspecies hydrogen transfer, and enables collective magnetotactic motility by the consortium.

Potential of direct interspecies electron transfer in synergetic enhancement of methanogenesis and sulfate removal in an up-flow anaerobic sludge blanket reactor with magnetite

Anaerobic digestion (AD) has been widely applied in the treatment of industrial wastewater containing oxidized sulfur compounds. However, the production of hydrogen sulfide usually limits the syntrophic metabolism proceeded by interspecies hydrogen transfer (IHT), due to its corrosive and toxic properties. The current study was in an attempt to establish direct interspecies electron transfer (DIET) to resist the toxic inhibition from hydrogen sulfide and keep syntrophic metabolism stable. The results showed that, in the presence of magnetite, the methane production was improved about 3–10 folds at each ratio of COD/SO42−, while the enhancement of methanogenesis had almost no negative effect on sulfate reduction. With magnetite, the sludge conductance increased about 3 folds, but the concentration of c-type cytochromes decreased, suggesting that the potential DIET via both electrically conductive pili and outer surface c-type cytochromes was established. Microbial community revealed that, Veillonella species, the Fe(III)-reducing genus capable of reducing sulfate to hydrogen sulfide, were specially enriched with magnetite. Together with the relatively higher abundance of Methanothrix and Methanosarcina species, the novel DIET between Fe(III)/sulfate-reducing genus and methanogens was inferred to be responsible for the synergetic enhancement of methanogenesis and sulfate removal.

Growth Kinetics, Carbon Isotope Fractionation, and Gene Expression in the Hyperthermophile Methanocaldococcus jannaschii during Hydrogen-Limited Growth and Interspecies Hydrogen Transfer.

Hyperthermophilic methanogens are often H2 limited in hot subseafloor environments, and their survival may be due in part to physiological adaptations to low H2 conditions and interspecies H2 transfer. The hyperthermophilic methanogen Methanocaldococcus jannaschii was grown in monoculture at high (80 to 83 μM) and low (15 to 27 μM) aqueous H2 concentrations and in coculture with the hyperthermophilic H2 producer Thermococcus paralvinellae The purpose was to measure changes in growth and CH4 production kinetics, CH4 fractionation, and gene expression in M. jannaschii with changes in H2 flux. Growth and cell-specific CH4 production rates of M. jannaschii decreased with decreasing H2 availability and decreased further in coculture. However, cell yield (cells produced per mole of CH4 produced) increased 6-fold when M. jannaschii was grown in coculture rather than monoculture. Relative to high H2 concentrations, isotopic fractionation of CO2 to CH4 (εCO2-CH4) was 16‰ larger for cultures grown at low H2 concentrations and 45‰ and 56‰ larger for M. jannaschii growth in coculture on maltose and formate, respectively. Gene expression analyses showed H2-dependent methylene-tetrahydromethanopterin (H4MPT) dehydrogenase expression decreased and coenzyme F420-dependent methylene-H4MPT dehydrogenase expression increased with decreasing H2 availability and in coculture growth. In coculture, gene expression decreased for membrane-bound ATP synthase and hydrogenase. The results suggest that H2 availability significantly affects the CH4 and biomass production and CH4 fractionation by hyperthermophilic methanogens in their native habitats.IMPORTANCE Hyperthermophilic methanogens and H2-producing heterotrophs are collocated in high-temperature subseafloor environments, such as petroleum reservoirs, mid-ocean ridge flanks, and hydrothermal vents. Abiotic flux of H2 can be very low in these environments, and there is a gap in our knowledge about the origin of CH4 in these habitats. In the hyperthermophile Methanocaldococcus jannaschii, growth yields increased as H2 flux, growth rates, and CH4 production rates decreased. The same trend was observed increasingly with interspecies H2 transfer between M. jannaschii and the hyperthermophilic H2 producer Thermococcus paralvinellae With decreasing H2 availability, isotopic fractionation of carbon during methanogenesis increased, resulting in isotopically more negative CH4 with a concomitant decrease in H2-dependent methylene-tetrahydromethanopterin dehydrogenase gene expression and increase in F420-dependent methylene-tetrahydromethanopterin dehydrogenase gene expression. The significance of our research is in understanding the nature of hyperthermophilic interspecies H2 transfer and identifying biogeochemical and molecular markers for assessing the physiological state of methanogens and possible source of CH4 in natural environments.

Bioconversion Pathway of CO2 in the Presence of Ethanol by Methanogenic Enrichments from Production Water of a High-Temperature Petroleum Reservoir

Transformation of CO2 in both carbon capture and storage (CCS) to biogenic methane in petroleum reservoirs is an attractive and promising strategy for not only mitigating the greenhouse impact but also facilitating energy recovery in order to meet societal needs for energy. Available sources of petroleum in the reservoirs reduction play an essential role in the biotransformation of CO2 stored in petroleum reservoirs into clean energy methane. Here, the feasibility and potential on the reduction of CO2 injected into methane as bioenergy by indigenous microorganisms residing in oilfields in the presence of the fermentative metabolite ethanol were assessed in high-temperature petroleum reservoir production water. The bio-methane production from CO2 was achieved in enrichment with ethanol as the hydrogen source by syntrophic cooperation between the fermentative bacterium Synergistetes and CO2-reducing Methanothermobacter via interspecies hydrogen transfer based upon analyses of molecular microbiology and stable carbon isotope labeling. The thermodynamic analysis shows that CO2-reducing methanogenesis and the methanogenic metabolism of ethanol are mutually beneficial at a low concentration of injected CO2 but inhibited by the high partial pressure of CO2. Our results offer a potentially valuable opportunity for clean bioenergy recovery from CCS in oilfields.

Bioelectrochemical Stimulation of Electromethanogenesis at a Seawater-Based Subsurface Aquifer in a Natural Gas Field

In subsurface anoxic environments, microbial communities generally produce methane as an end-product to consume organic compounds. This metabolic function is a source of biogenic methane in coastal natural gas aquifers, submarine mud volcanoes and methane hydrates. Within the methanogenic communities, hydrogenotrophic methanogens and syntrophic bacteria are converting volatile fatty acids to methane syntrophically via interspecies hydrogen transfer. Recently, direct interspecies electron transfer (DIET) between fermentative/syntrophic bacteria and electrotrophic methanogens has been proposed as an effective interspecies metabolite transfer process to enhance methane production. In this study, in order to stimulate the DIET-associated methanogenic process at deep biosphere-aquifer systems in a natural gas field, we operated a bioelectrochemical system (BES) to apply voltage between an anode and a cathode. Two single-chamber BESs were filled with seawater-based formation water collected from an onshore natural gas well, repeatedly amended with acetate, and operated with 600 mV between electrodes for 21 months, resulting in a successful conversion of acetate to methane via electrical current consumption. One reactor yielded a stable current by ~200 mA/m2 with a coulombic efficiency (CE) of >90%; however, the other reactor, which had been incidentally disconnected for 3 days, showed less electromethanogenic activity with a CE of only ~10%. The 16S rRNA gene-based community analyses showed that two methanogenic archaeal families, Methanocalculaceae and Methanobacteriaceae, were abundant in cathode biofilms that were mainly covered by single-cell-layered biofilm, implicating them as key players in the electromethanogenesis. In contrast, family Methanosaetaceae was abundant at both electrodes and the electrolyte suspension only in the reactor with less electromethanogenesis, suggesting this family was not involved in electromethanogenesis and only activated after the no electron flow event. On anodes covered by thick biofilms with filamentous networks, family Desulfuromonadaceae dominated in the early stage of the operation, while family Geobacteraceae (mainly genus Geoalkalibacter) increased their frequencies during the longer-term operation, which indicates that these families were correlated with electrode-respiring reactions. These results indicate that the BES reactors with voltage application effectively activated a subsurface DIET-related methanogenic microbiome in the natural gas field, and specific electrogenic bacteria and electromethanogenic archaea were identified within the anode and/or cathode biofilms.

Insights into direct interspecies electron transfer mechanisms for acceleration of anaerobic digestion of wastes

Anaerobic digestion of waste organic biomass is a well-known process for conversion of biomass to bioenergy. However, global acceptability of anaerobic digestion process for renewable energy has often been undermined because of its inconsistent or lower rate of biogas production. The overall success of the anaerobic digestion process considerably depends on the interactions between the microbial communities within the digester. The electron transfer through interspecies hydrogen transfer between acetogens and methanogens is a major bottleneck for successful anaerobic digestion process. Recent studies have reported the role of biotic (pili and cytochromes) as well as abiotic (conductive materials) components to accelerate direct interspecies electron transfer between microbial communities in the digester. These transfer mechanisms via biotic components of exoelectrogenic bacteria and methanogenic archaea is thermodynamically more favorable over indirect interspecies electron transfer. However, the use of conductive materials in promoting anaerobic digestion process has been an area of research in the last few years. The process of direct interspecies electron transfer promoting anaerobic digestion process has been investigated evidently in pure microbial cultures; however, there are scanty reports on existence of direct interspecies electron transfer in mixed microbial consortia. The present review highlights the fundamentals and applications of direct interspecies electron transfer-promoted anaerobic digestion process at laboratory scale studies used to improve kinetics of methanogenesis.

Methane Production and Conductive Materials: A Critical Review.

Conductive materials (CM) have been extensively reported to enhance methane production in anaerobic digestion processes. The occurrence of direct interspecies electron transfer (DIET) in microbial communities, as an alternative or complementary to indirect electron transfer (via hydrogen or formate), is the main explanation given to justify the improvement of methane production. Not disregarding that DIET can be promoted in the presence of certain CM, it surely does not explain all the reported observations. In fact, in methanogenic environments DIET was only unequivocally demonstrated in cocultures of Geobacter metallireducens with Methanosaeta harundinacea or Methanosarcina barkeri and frequently Geobacter sp. are not detected in improved methane production driven systems. Furthermore, conductive carbon nanotubes were shown to accelerate the activity of methanogens growing in pure cultures, where DIET is not expected to occur, and hydrogenotrophic activity is ubiquitous in full-scale anaerobic digesters treating for example brewery wastewaters, indicating that interspecies hydrogen transfer is an important electron transfer mechanism in those systems. This paper presents an overview of the effect of several iron-based and carbon-based CM in bioengineered systems, focusing on the improvement in methane production and in microbial communities' changes. Control assays, as fundamental elements to support major conclusions in reported experiments, are critically revised and discussed.