Interval interpolation formulae play a significant role to find the value of an unknown function at some points under interval uncertainty. The objective of this paper is to establish Newton’s divided interpolation formula for interval-valued functions using generalized Hukuhara difference of intervals. For this purpose, arithmetic of intervals, Hukuhara difference and its some properties and concept of interval-valued function have been discussed briefly. Using Hukuhara difference of intervals, the definition of Newton’s divided gH-difference for interval-valued function has been introduced. Then Newton’s divided gH-differences interpolation formula has been derived. Finally, with the help of some numerical examples, the proposed interpolation formula has been illustrated.