It is important to choose the appropriate interocclusal registration material for precise articulation and successful dental prosthesis fabrication. 3 types of interocclusal registration materials: Bite registration wax, polyvinyl siloxane bite registration paste and Bisacryl-based bite registration paste were evaluated for dimensional stability and surface hardness at 4 different time intervals. One way ANOVA test, multiple Post Hoc Tukey HSD test and nonparametric test were performed. Bisacryl-based bite registration material exhibited better dimensional stability and surface hardness than polyvinyl siloxane and bite registration wax at all 4 time intervals. Thus dimensional stability and surface hardness of interocclusal registration material was influenced by both the type of material and the time duration.