Abstract Funding Acknowledgements no OnBehalf no Background Quantitative analysis of deformation parameters can provide evidence about the presence of disease . Speckle tracking (ST)strain is recently largely used in sports medicine, as an usefull tool to add value to the traditional echo information .Especially 4D echo-strain is emerging in this context despite not yet completely explored. The study aim to verify the feasibility of the 4D strain analysis , compared to the 2D ST strain data, in a class of international trained soccer referee. Materials and methods : a group of 25 soccer referee ( aged 45,5 ± 5) underwent to an EMT, conducted at the 85% of their effort to obtain the eligibility. An 2D echocardiographic evaluation was also performed with the measurements of the systolic-diastolic parameters . A specific investigation for the 2D and 4D strain analysis by X-Strain-Esaote software, was possible from the 2C,3C;4C apical view chambers, acquired as cine loop. The study was completed by the study of the circumferential strain calculated from short axis view, from the images of the basal and medium LV segments. Results : all 2D echo parameters were normal (LVDD: 50,56 ± 2 mm,68; LVSD: 35,4 ± 2,3 mm; EF: 65,76 ±1,78%; E/A:1,73 ± 0,35 ; RV:23,71 ± 1 mm) The 2D GLS ( -22,29 ± 2,64); Circumferential strain( Basal -22,29 ± 5,3; Apical -25,66 ± 5,8 ) were in the normal range. No statistical differences ( P:NS) were observed comparing the mean GLS(21,60 ± 2,68) to the LV 4D strain( -21,24 ± 2,75) Conclusions : 4D strain completes and strengthens the role of the 2D strain in evaluating the myocardial function contributing to exclude eventual undiscovered disease. Especially in athletes, often checked in a short time the combination of the two methods should be widely proposed .More studies will be necessary in future to confirm this hypothesis. Abstract P1534 Figure. 4D strain