International Comparison Tests Research Articles

An apparatus and a method for determining the slip resistance of shoes and floors by simulation of human foot motions.

An apparatus to measure the coefficient of kinetic friction (mu k) between the shoe sole and the underfoot surface was constructed, and a method including criteria to evaluate the risk of slipping during walking was developed. The apparatus is a prototype stationary step simulator capable of simulating the movements of a human foot and the forces applied to the underfoot surface during an actual slip, and the drainage capability of the contact surface between the shoe sole and the flooring when different lubricants or contaminants are used. The apparatus consists of a movable artificial foot controlled by a computer with the aid of three hydraulic cylinders. The frictional force (F mu), the normal force (FN) and their ratio (mu k = F mu/FN) are measured with a two-way force platform when the foot slides along its surface. Two separate gait patterns, heel-side (mu k 1) and sole-slide (mu k 2) gait pattern, are used for the evaluations. The method classifies studied shoe, lubricant and underfoot surface combinations into five slip resistance classes according to the measured mu k 1. The slip resistance assessments are specified with some complementary safety criteria, e.g., the ratio mu k 1/mu k 2. The reliability of the developed measurement method was assessed in an international comparison test. According to available results discussed in this paper, our method seems to be valid and the slip resistance measurements seem to be repeatable.

Immunomagnetic isolation of cells for serological BoLA typing.

This paper describes a totally new immunomagnetic (IM) technique adapted to serological BoLA typing. The basic technique has recently been developed by Vartdal et al. (1986) for serological HLA typing. The main advantage is that bovine mononuclear cells (e.g. T-cells and possibly their subsets, B-cells and monocytes) can be quickly and specifically isolated with high yield and viability from whole blood in a one-step procedure. This is achieved by magnetic separation of rosettes formed between the cells and superparamagnetic monosized polystyrene microspheres (Dynabeads TM) coated with cross-species reactive monoclonal antibodies (MAbs) specific for various human T-cell antigens or for HLA class II monomorphic epitopes. The cells are isolated within 5 min after a 5-min incubation at 4 degrees C. Magnetic separation of rosettes with a strong cobalt-samarium magnet eliminates all the laborious centrifugation steps necessary with conventional procedures. The isolated cells, still attached to the particles, are available for microcytotoxic assay. This is carried out within 55 min, including a two-step application of alloantiserum and complement and addition of acridine orange/ethidium bromide for the staining of viable (green) and dead (red) cells. The high viability of isolated cells gives a very low background kill compared with the conventional cytotoxic assay. The IM typing technique is also superior in sensitivity to the conventional technique as standardized for the international BoLA comparison test. The IM technique is likely to have its greatest impact on class II typing; class II positive cells being separated very efficiently. Polymorphic HLA class II MAbs detected likely polymorphic BoLA class II epitopes.

Joint report of the first international comparison test on swine lymphocyte alloantigens (SLA).

The first international comparison test on swine lymphocyte alloantigens (SLA) was held in Helsinki, Finland in July 1986. The results reported were based on a comparison of 157 alloantisera originating from six laboratories. The antisera were tested against a selected panel of 264 lymphocyte samples belonging to four laboratories. The most common breeds in Europe were chosen for this first comparison test (Landrace and Large White). Eighteen of the 31 previously known specificities were confirmed and a new nomenclature was established.

Report on the Third International Comparison Test for Sheep Blood Typing Reagents and for Red Cell and Serum Protein and Enzyme Polymorphisms

Report on the Third International Comparison Test for Sheep Blood Typing Reagents and for Red Cell and Serum Protein and Enzyme Polymorphisms

Compatibility of Meteorological Rocketsonde Data as Indicated by International Comparison Tests

Results of two experiments involving intercomparisons of rocketsonde measurements of temperature and wind data in the 25 to 80 km altitude region in the CIMO (Commission for Instruments and Methods of Observations) program during September 1973 are reported. Rocketsonde systems used by France, the USSR, the UK, the U.S., and Japan in the two parts of the program (one series of tests at Wallops Island, Va., the other in French Guiana) are described. Wind data were derived from radar tracking of retardation devices and payloads in descent. Day and night temperature differences were examined. The intercomparisons revealed excellent compatibility of the rocketsonde data up to 60 km in wind observations. Some outstanding problems are pointed out.

豚の血液型未同定抗血清の国際比較試験による血液型システムの決定

豚の血液型未同定抗血清の国際比較試験による血液型システムの決定