You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Prostate & Genitalia1 Apr 2010811 A COMPARATIVE PHARMACOKINETIC STUDY OF SEVEN BRANDS OF CIPROFLOXACIN IN NZW RABBITS USING LIQUID CHROMATOGRAPHY MASS SPECTROMETRY TECHNIQUE Elijah Kehinde, Mohammed Abdel-Hamid, Oludotun Philips, Leyla Sharaf, and Alice Babu Elijah KehindeElijah Kehinde Safat, Kuwait More articles by this author , Mohammed Abdel-HamidMohammed Abdel-Hamid Kuwait, Kuwait More articles by this author , Oludotun PhilipsOludotun Philips Kuwait, Kuwait More articles by this author , Leyla SharafLeyla Sharaf Kuwait, Kuwait More articles by this author , and Alice BabuAlice Babu Kuwait, Kuwait More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1487AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The quinolones have been used widely in urological practice as prophylactic antibiotics prior to TRUS biopsy of the prostate and in the treatment of established susceptible uropathogens. Increasing resistance to the quinolones especially ciprofloxacin (CIP) is becoming a common problem in many urology units for possibly many reasons including the widespread use of generic substitutes. In this study we evaluated the pharmacokinetic behavior and the bioavailability of six generic brands of CIP in comparison to a reference branded product in NZW rabbits. METHODS A rapid and selective positive electrospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for the determination of the total concentration of ciprofloxacin (unbound and protein-bound) in rabbit serum was applied. The developed method was conducted using XTerraR RP C8 column and a mobile phase of acetonitrile/water/formic acid in a ratio 80:20:0.025v/v/v at a flow rate 0.4 ml/min. Sample analysis was completed within 5 minutes. A preliminary protein precipitation procedure was applied to clean up the serum samples before analysis. Quantification was achieved by internal standard calibration using rufloxacin (RUF) as an internal standard (IS). Detection of CIP and IS was achieved using multiple reaction monitoring (MRM) at m/z 332.7>245.5 and m/z 364.7>263.2, respectively. Pharmacokinetics of CIP were evaluated in NZW rabbits following intra-gastric administration of 7 CIP brands. Generic CIP products used were obtained from the USA, Canada, UK, Egypt, Kuwait and Nigeria. RESULTS The developed method showed a good linearity (r >0.99), precision (RSD<6.1%) and accuracy (Bias <8.5%) over the concentration range of 0.5-6 μg/ml with a lower limit of quantification of 0.5 μg/ml and recovery between 93.5 and 108.5%. The specificity was proved by the absence of interference of endogenous serum constituents. Pharmacokinetic studies indicated that the % relative bioavailability of the generic brands was 33.1 to 77.5% compared to the branded reference product. CONCLUSIONS This study showed that there is a wide variation in the absorption patterns and possibly the therapeutic efficacy of the generic products of CIP compared to the branded reference product. Part of the explanation for increasing bacterial resistance to CIP may be due to the widespread use of generic CIP products with reduced efficacy and safety. © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byKehinde E, Al-Maghrebi M, Sheikh M and Anim J (2018) Combined Ciprofloxacin and Amikacin Prophylaxis in the Prevention of Septicemia After Transrectal Ultrasound Guided Biopsy of the ProstateJournal of Urology, VOL. 189, NO. 3, (911-915), Online publication date: 1-Mar-2013. Volume 183Issue 4SApril 2010Page: e316-e317 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.Keywordsrabbit serumliquid chromatographymass spectrometryCiprofloxacinpharmacokineticMetricsAuthor Information Elijah Kehinde Safat, Kuwait More articles by this author Mohammed Abdel-Hamid Kuwait, Kuwait More articles by this author Oludotun Philips Kuwait, Kuwait More articles by this author Leyla Sharaf Kuwait, Kuwait More articles by this author Alice Babu Kuwait, Kuwait More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...