Internal Region Research Articles

A Molecular Dynamics Simulation of Complexes of Fullerenes and Lysine-Based Peptide Dendrimers with and without Glycine Spacers.

The development of new nanocontainers for hydrophobic drugs is one of the most important tasks of drug delivery. Dendrimers with hydrophobic interiors and soluble terminal groups have already been used as drug carriers. However, the most convenient candidates for this purpose are peptide dendrimers since their interiors could be modified by hydrophobic amino acid residues with a greater affinity for the transported molecules. The goal of this work is to perform the first molecular dynamics study of the complex formation of fullerenes C60 and C70 with Lys-2Gly, Lys G2, and Lys G3 peptide dendrimers in water. We carried out such simulations for six different systems and demonstrated that both fullerenes penetrate all these dendrimers and form stable complexes with them. The density and hydrophobicity inside the complex are greater than in dendrimers without fullerene, especially for complexes with Lys-2Gly dendrimers. It makes the internal regions of complexes less accessible to water and counterions and increases electrostatic and zeta potential compared to single dendrimers. The results for complexes based on Lys G2 and Lys G3 dendrimers are similar but less pronounced. Thus, all considered peptide dendrimers and especially the Lys-2Gly dendrimer could be used as nanocontainers for the delivery of fullerenes.

Boundary controllability of Riemann–Liouville fractional semilinear equations

We study the boundary regional controllability of a class of Riemann–Liouville fractional semilinear sub-diffusion systems with boundary Neumann conditions. The result is obtained by using semi-group theory, the fractional Hilbert uniqueness method, and Schauder’s fixed point theorem. Conditions on the order of the derivative, internal region, and on the nonlinear part are obtained. Furthermore, we present appropriate sufficient conditions for the considered fractional system to be regionally controllable and, therefore, boundary regionally controllable. An example of a population density system with diffusion is given to illustrate the obtained theoretical results. Numerical simulations show that the proposed method provides satisfying results regarding two cases of the control operator.

Histological and ultrastructural features of the peculiar seta and stem of Leucodon canariensis (Leucodontaceae)

The seta and stem in Leucodon canariensis are examined by means of light microscopy, transmission and scanning electron microscopy. Their anatomies reveal relevant differences although both show a remarkable structural complexity, as their conducting tissues are equally effective in carrying out essential processes. Five types of cells have been recognized in the transverse sections: in both seta and stem: (i) stereids in the external region with a peripheral cuticle; in the stem only: (ii) parenchymatous cells with plasmodesmata underlying the stereids; in the seta only: (iii) an unusual area of nacreous-walled cells without live protoplasm, surrounding (iv) the food-conducting cells (leptoid-like), and again both in seta and stem: (v) hydroids in the internal region. The seta in this species has a peculiar organization and shares some characteristics with polytrichaceous mosses; the ultrastructural similarities and differences and functional significance of these cells are discussed, both systematically and in relation to the habit of the moss itself.

A comparative evaluation of internal and marginal fits of custom post and core fabricated using conventional and two digital techniques: An in vitro study.

The purpose of this in vitro study was to comparatively evaluate the marginal and internal fits of cobalt-chromium metal custom post and core fabricated using a conventional technique with two digital techniques. The study was designed in an in-vitro study setting. Five sets of custom post and core restorations were fabricated using the conventional (Group 1) and two semi digital methods (digital scanning of the resin pattern and computer aided additive manufacturing, and digital scanning of the silicone impression and subsequent computer aided designing [CAD] computer aided manufacturing fabrication) (Group 2 and 3). Marginal and internal fits of the posts were evaluated using a micro computed tomography scan at various points. A one way ANOVA test of the scores was made to evaluate the effect of different methods of custom post and core fabrication on marginal and internal fits. Bonferroni adjusted post hoc tests were conducted for intergroup comparison. Least marginal gap was reported in Group 3 (82.5 ± 14.36 μm) followed by Group 1 (110 ± 25.19 μm) and Group 2 (112.5 ± 26.75 μm). Least internal gap at cervical, middle and apical as well as overall values were observed in Group 3 (78 ± 9.25 μm, 72 ± 7.79 μm, 160 ± 15.81 μm, 103.3 ± 4.43 μm) followed by Group 1 (113.5 ± 25.35 μm, 132.5 ± 19.92 μm, 502 ± 74.63 μm, 249.3 ± 25.44 μm) and Group 2 (114.5 ± 21.68 μm, 133.5 ± 19.57 μm, 598 ± 87.86 μm, 282 ± 28.91 μm) respectively. The results of one-way ANOVA and Bonferroni adjusted post hoc tests for marginal gap did not show any statistically significant difference between the three groups (P > 0.05) but revealed statistically significant difference (P = 0.02) in internal gap values at the cervical, middle, and apical regions as well as overall internal gap region between the three groups. Better marginal and internal fits were observed in custom post and core fabricated by digital scanning of the silicone impression and subsequent CAD as compared to those fabricated by the other two groups.

Local Contractive Registration with Biomechanical Model: Assessing Microwave Ablation after Compensation for Tissue Shrinkage.

Microwave ablation (MWA) is a minimally invasive procedure for the treatment of liver tumor. Accumulating clinical evidence has considered the minimal ablative margin (MAM) as a significant predictor of local tumor progression (LTP). In clinical practice, MAM assessment is typically carried out through image registration of pre- and post-MWA images. However, this process faces two main challenges: non-homologous match between tumor and coagulation with inconsistent image appearance, and tissue shrinkage caused by thermal dehydration. These challenges result in low precision when using traditional registration methods for MAM assessment. In this paper, we present a local contractive nonrigid registration method using a biomechanical model (LC-BM) to address these challenges and precisely assess the MAM. The LC-BM contains two consecutive parts: (1) local contractive decomposition (LC-part), which reduces the incorrect match between the tumor and coagulation and quantifies the shrinkage in the external coagulation region, and (2) biomechanical model constraint (BM-part), which compensates for the shrinkage in the internal coagulation region. After quantifying and compensating for tissue shrinkage, the warped tumor is overlaid on the coagulation, and then the MAM is assessed. We evaluated the method using prospectively collected data from 36 patients with 47 liver tumors, comparing LC-BM with 11 state-of-the-art methods. LTP was diagnosed through contrast-enhanced MR follow-up images, serving as the ground truth for tumor recurrence. LC-BM achieved the highest accuracy (97.9%) in predicting LTP, outperforming other methods. Therefore, our proposed method holds significant potential to improve MAM assessment in MWA surgeries.

Research on the deformation characteristics of small module tooth during current-assisted splitting spinning of small module tooth-shaped part

Aiming at the problems such as low material utilization, poor strength and toughness of workpiece during manufacturing of small module tooth-shaped parts (SMTSPs) by traditional gear hobbing and shaping method, a current-assisted splitting spinning forming (CASSF) method was proposed to form SMTSPs with high performance requirements. A coupled electrical-thermal-mechanical finite element (FE) model was established based on the ABAQUS software, and the distribution of equivalent stress and equivalent strain, and deformation characteristics of small module tooth were investigated. The results show that the equivalent stress and equivalent strain decrease from tooth groove arc to the tooth crest arc and centre of the tooth groove at tooth profile surface. The distribution of equivalent stress and equivalent strain in the internal deformation region follows the same trend as the tooth profile surface from the centre of the tooth groove to the tooth groove arc, but increases from the tooth groove arc to the tooth crest. The material of small module tooth experiences three-directional stress state. The material in the gear tooth undergoes radial and axial elongation, and tangential compression, while in the tooth groove it undergoes radial compression, tangential and axial elongation.

R-MATRIX CALCULATIONS OF THE DEUTERIUM-TRITIUM FUSION CROSS SECTION BASED ON PRECISE COULOMB FUNCTIONS

Due to the availability of fuel, favorable kinetics, and high output energy, the deuterium-tritium (D-T) fusion reaction is dominantly used in thermonuclear fusion. This paper presents a detailed, step-by-step theoretical calculation of the D-T fusion cross-section within the framework of the phenomenological R-matrix method.The fundamental principles of the phenomenological R-matrix method are outlined.Nuclear and Coulomb interactions are addressed by the R-matrix formalism, which classifies the configuration space into internal and external regions, respectively.Precise Coulomb functions are utilized in the calculations, essential for the accurate determination of the penetration and shift factors.Precise Coulomb functions for the D+T,4He+12C systems have been calculated. The penetration and shift factors for the D+T system for a given angular momentum have been obtained.Two different R-matrix models with parameters from recent scientific papers are employed to calculate D-T fusion cross-sections and reaction rates, which are then compared with those obtained from the ENDF/B-VIII.0 library data. Within a crucial low-energy range (from 0 to 0.1 MeV), important for fusion technology, our results show quite good agreement with experimental data, while in a high-energy range, the obtained results are slightly underestimated due to non-resonant 5He levels not being taken into account. These findings indicate that the models can be used for future thermonuclear fusion applications.

Justice in the Policy of Legal Acculturation of Siberian Peoples (First Half of the 19th Century)

The article examines the issue of extending Russian legal standards to the indigenous population of Siberia through the jurisdictional activities of imperial authorities. At the same time, an acculturation model is used to explain the relations between the imperial center and the internal peripheral regions and outskirts of the state, which previous historiography has not dealt with. The scientific analysis was carried out using regulatory legal acts and office documentation, mainly re-extracted from domestic state archives. Government measures aimed at the legal acculturation and integration of Siberian indigenous peoples into the general imperial legal system through the use of flexible forms of judicial system and legal proceedings are considered. The living conditions of the “foreigners” had significant geographical, cultural, and economic differences. This was taken into account by the imperial legislator, who offered the aborigines extraordinary bodies of justice. However, social life made traditional forms of court extremely viable, which, together with other factors, increased the diversity of judicial institutions and procedures in the Siberian macro-region.

Improved dark channel priori single image defogging technique using image segmentation and joint filtering.

Foggy images affect image analysis and measurement because of their low definition and blurred details. Despite numerous studies on haze in natural images in hazy environments, the recovery effect is not ideal for processing hazy images in sky areas. A dark channel priori technique for processing haze images with sky areas where atmospheric light values are misestimated and halo artefacts are produced, as well as an improved dark channel priori single-image defogging technique based on image segmentation and joint filtering, are proposed. First, an estimation method of the atmospheric illumination value using image segmentation is proposed to obtain the atmospheric illumination value. The probability density distribution function of the haze-grey image was constructed during image segmentation. The probability density distribution function of the grey image value, the K-means clustering technique, and the method for estimating atmospheric illumination values are combined to improve image segmentation techniques and achieve the segmentation of sky and non-sky areas in hazy images. Based on the segmentation threshold, the number of pixels in the sky and non-sky areas, as well as the normalisation results, were counted to calculate the atmospheric illumination values. Second, to address the halo artefact phenomenon, a method for optimising the image transmittance map using joint filtering is proposed. The image transmittance map was optimised by combining fast-guided filtering and weighted least-squares filtering to retain the edge information and smooth the gradient change of the internal region. Finally, gamma correction and automatic level optimisation are used to improve the brightness and contrast of the defogged images. The experimental results show that the proposed technique can effectively achieve sky segmentation. Compared to the traditional dark-channel prior technique, the proposed technique suppress halo artefacts and improve image detail recovery. Compared to other techniques, the proposed technique exhibited excellent performance in subjective and objective evaluations.

A new small molecule DoNA binding to CAG repeat RNA

We here report a new molecule DoNA binding to a CAG repeat RNA. DoNA is a dimer of the NA molecule that we previously reported. NA binds with high affinity to a CAG repeat DNA but not significantly to a CAG repeat RNA. Binding analyses using SPR and CSI-TOF MS indicated a significant increase in the affinity of DoNA to a single stranded CAG repeat RNA compared to NA. Systematic investigation of the RNA motifs bound by DoNA using hairpin RNA models revealed that DoNA binds to the CAG units at overhang and terminal positions, and notably, it binds to the structurally flexible internal and hairpin loop region.

Hexagonal plate ultrasound pretreatment on the correlation between soy protein isolate structure and cholesterol-lowering activity of peptides, and protein's enzymolysis kinetics, thermodynamics

In this study, a hexagonal plate ultrasound (HPU) pretreatment technology was employed to modify soy protein isolate (SPI) and enhance the hypocholesterolemic activity of enzymatic digests from SPI. Results demonstrated that under the condition of ultrasound power density of 40 W/L, the hypocholesterolemic activity of enzymatic digests from HPU-pretreated SPI (HPU-SPI) increased by 88.40 % compared to control group after gastrointestinal digestion. The sulfhydryl content of HPU-SPI increased by a maximum of 45.32 % compared to control group. Fourier transform infrared and scanning electron microscopy revealed that HPU pretreatment partially unfolded the SPI conformation, reduced the intermolecular interactions, and exposed the internal hydrophobic regions. Pearson correlation analysis showed that sulfhydryl groups (r = 0.860), disulfide bonds (r = −0.875) and random coil (r = 0.917) were strongly correlated with the cholesterol-lowering activity of soy protein hydrolysate (SPH), following a simulated gastrointestinal digestion. Finally, the effects of HPU pretreatment on enzymolysis kinetics and thermodynamics of the SPI enzymatic process showed that HPU pretreatment significantly reduced the Mie's constant, activation energy, activation enthalpy, activation entropy and Gibbs free energy. Overall, the study outcome suggested that HPU pretreatment could positively influence the hypocholesterolemic peptide activity, and thus, may be beneficial to the pharmaceutical/food industry.

Spheroid size influences cellular senescence and angiogenic potential of mesenchymal stromal cell-derived soluble factors and extracellular vesicles.

Introduction: The secretome of mesenchymal stromal cells (MSCs) serves as an innovative tool employed in the regenerative medicine approach. In this particular context, three-dimensional (3D) culture systems are widely utilized to better replicate in vivo conditions and facilitate prolonged cell maintenance during culture. The use of spheroids enables the preservation of the classical phenotypical characteristics of MSCs. However, the distinct microenvironment within the spheroid may impact the secretome, thereby enhancing the angiogenic properties of adult MSCs that typically possess a reduced angiogenic potential compared to MSCs derived from perinatal tissues due to the hypoxia created in the internal region of the spheroid. Methods: In this study, large spheroids (2,600 cells, ∼300μm diameter) and small spheroids (1,000 cells, ∼200μm diameter) were used to examine the role of spheroid diameter in the generation of nutrients and oxygen gradients, cellular senescence, and the angiogenic potential of secreted factors and extracellular vesicles (EVs). Results: In this study, we demonstrate that large spheroids showed increased senescence and a secretome enriched in pro-angiogenic factors, as well as pro-inflammatory and anti-angiogenic cytokines, while small spheroids exhibited decreased senescence and a secretome enriched in pro-angiogenic molecules. We also demonstrated that 3D culture led to a higher secretion of EVs with classical phenotypic characteristics. Soluble factors and EVs from small spheroids exhibited higher angiogenic potential in a human umbilical vein endothelial cell (HUVEC) angiogenic assay. Discussion: These findings highlighted the necessity of choosing the appropriate culture system for obtaining soluble factors and EVs for specific therapeutic applications.

Distinct modes of telomere synthesis and extension contribute to Alternative Lengthening of Telomeres

Alternative lengthening of telomeres (ALT) is a homology-directed repair mechanism that becomes activated in a subset of cancers to maintain telomere length. One of the defining features of ALT cells is the prevalence of extrachromosomal telomeric repeat (ECTR) DNA. Here, we identify that ALT cells engage in two modes of telomere synthesis. Non-productive telomere synthesis occurs during the G2 phase of the cell cycle and is characterized by newly synthesized internal telomeric regions that are not retained in the subsequent G1, coinciding with an induction of ECTR DNA. Productive telomere synthesis occurs specifically during the transition from G2 to mitosis and is defined as the extension of the telomere termini. While many proteins associated with break-induced telomere synthesis function in both non-productive and productive telomere synthesis, POLH specifically promotes productive telomere lengthening and suppresses non-productive telomere synthesis. These findings delineate the mechanism and cell cycle regulation of ALT-mediated telomere synthesis and extension.

Relaxation Annealing Influence on the Magnetic Properties and Magnetic Impedance of Amorphous Co-Based Wires

Abstract—The results of a study of the influence of the 2 hours relaxation annealing at a temperature of 620 K on the magnetoimpedance effect (MI) in amorphous Co66Fe4Nb2.5Si12.5B15 wires are presented. It was found that MI at low ac frequencies after heat treatment increases noticeably, while it changes slightly at high frequencies. Using magneto-impedance tomography, it is shown that this is due to the fact that the changes in magnetic properties caused by heat treatment are not the same in different regions of the wire. Thus, in the surface region with a thickness of about 2.5 μm, the magnetic permeability remains almost unchanged, but in the internal regions it increases significantly after annealing.

Effects of oxygen on the optical properties of phenyl-based scintillators during irradiation and recovery

Plastic scintillators are a versatile and inexpensive option for particle detection, which is why the largest particle physics experiments, CMS and ATLAS, use them extensively in their calorimeters. One of their challenging aspects, however, is their relatively low radiation hardness, which might be inadequate for very high luminosity future projects like the FCC-hh. In this study, results on the effects of ionizing radiation on the optical properties of plastic scintillator samples are presented. The samples are made from two different matrix materials, polystyrene and polyvinyltoluene, and have been irradiated at dose rates ranging from 2.2 Gy/h up to 3.4 kGy/h at room temperature. An internal boundary that separates two regions of different indices of refraction is visible in the samples depending on the dose rate, and it is compatible with the expected oxygen penetration depth during irradiation. The dose rate dependence of the oxygen penetration depth for the two matrix materials suggests that the oxygen penetration coefficient differs for PS and PVT. The values of the refractive index for the internal regions are elevated compared to those of the outer regions, which are compatible with the indices of unirradiated samples.

Unraveling blunt-end RNA binding and ATPase-driven translocation activities of the RIG-I family helicase LGP2.

The RIG-I family helicases, comprising RIG-I, MDA5and LGP2, are cytoplasmic RNA sensors that trigger an antiviral immune response by specifically recognizing foreign RNAs. While LGP2 lacks the signaling domain necessary for immune activation, it plays a vital role in regulating the RIG-I/MDA5 signaling pathway. In this study, we investigate the mechanisms underlying this regulation by examining the oligomeric state, RNA binding specificity, and translocation activity of human LGP2 and the impact of ATPase activity. We show that LGP2, like RIG-I, prefers binding blunt-ended double-stranded (ds) RNAs over internal dsRNA regions or RNA overhangs and associates with blunt-ends faster than with overhangs. Unlike RIG-I, a 5'-triphosphate (5'ppp), Cap0, or Cap1 RNA-end does not influence LGP2's RNA binding affinity. LGP2 hydrolyzes ATP in the presence of RNA but at a 5-10 fold slower rate than RIG-I. Nevertheless, LGP2 uses its ATPase activity to translocate and displace biotin-streptavidin interactions. This activity is significantly hindered by a methylated RNA patch, particularly on the 3'-strand, suggesting a 3'-strand tracking mechanism like RIG-I. The preference of LGP2 for blunt-end RNA binding, its insensitivity to Cap0/Cap1 modification, and its translocation/protein displacement ability have substantial implications for how LGP2 regulates the RNA sensing process by MDA5/RIG-I.

Effect of Electrodeposited Gold Coatings on Micro-Gaps, Surface Profile and Bacterial Leakage of Cast UCLA Abutments Attached to External Hexagon Dental Implants

Purpose: The objective of the study was to qualitatively assess the micro-gap dimensions, connecting fitting surface profile, and bacterial leakage of cast high-gold-alloy UCLA abutments, with or without electrodeposited gold coatings attached to external hexagon implants. Materials and methods: Sixteen plastic UCLAs (PUCLAs) were cast with a high-gold-content alloy. Eight were electrolytically gold plated. Five machined cast-to-UCLA (GUCLA) control abutments were cast with the same alloy. All abutments were attached to external hexagon implants, giving 21 implant-abutment combinations (IACs). External perimeter micro-gaps measured with SEM under shadow eliminating silhouette illumination and 2000× magnification were averaged over three regions. The IACs were examined for E. coli leakage following an initial sterility test. Disassembled combinations were examined with SEM, and surface profiles were qualitatively assessed. Results: External micro-gap measurements did not reflect the variable connecting surface profiles, but average values < 5.0 μm were observed for all IACs measured under the shadow eliminating silhouette illumination for both cast and pre-machined external hexagon abutments with and without Au plating. E. coli transfer was observed in 3 of 5 PUCLA-plated and 2 of 5 PUCLA-non-plated IACs. No transfer occurred in the 3 GUCLA-non-plated or 2 GUCLA-plated control IACs. Abutment connecting surfaces, both Au-plated and not Au-plated, showed plastic deformation (smearing) in variable mosaic patterns across the micro-gap. Conclusions: Micro-gap dimensions < 5μm were obtained with both the high noble metal cast and pre-machined control external hexagon abutments with and without Au electrodeposited on the abutment connecting surface. Regions of intimate contact due to plastic deformation (smearing) of these surfaces were observed. A continuous smeared region around the circumference of the surfaces can provide an effective barrier to the egress of E. coli bacteria from the internal regions of the implant under static loading. The sample size was insufficient to determine if the gold coating resulted in a superior bacterial barrier.

Internal wave activity in the deep Gulf of Mexico

Internal wave activity in the Gulf of Mexico (GoM) is investigated using a fleet of profiling floats. The floats continuously measured temperature and salinity as they drifted at a parking depth of 1500 dbar, allowing for the reconstruction of 2615 time series of isopycnal displacements. Thanks to the dense sampling of the eastern part of the GoM (east of 90°W), the geographical distribution of the internal waves displacement variance and available potential energy (APE) is revealed. The Loop Current (LC) influence region, between the Yucatan shelf to the west and the southern West Florida shelf to the east exhibits increased displacement variance and APE both in the continuum and near-inertial bands, while the north-eastern and central GoM show reduced internal wave activity. As the LC position fluctuates between a retracted and extended mode, we assessed the impact of the presence or absence of the LC in the increased internal wave activity region. It is shown that in the LC influence region, APE is increased (decreased) when the LC is present (absent), suggesting a strong control of the LC on deep internal waves activity. The 1500 dbar flow velocity, bottom roughness, and float altitude also seem to contribute to increased internal waves APE, but their influence is more subtle. Oppositely, no correlation with wind speed or wind intermittency is found.

Limb histology of the Triassic stem turtles Proterochersis porebensis Szczygielski & Sulej, 2016 and Proganochelys quenstedtii Baur, 1887 with insights into growth patterns of early turtles

Data on turtle limb bone histology and microstructure are spotty, especially for Mesozoic taxa, despite significant progress made in recent years. Here we provide first detailed information on the stylopodia of the Late Triassic stem turtles Proganochelys quenstedtii Baur, 1887 from Switzerland and Proterochersis porebensis Szczygielski & Sulej, 2016 from Poland. In both taxa we observed large, internal medullary regions filled with endosteal trabeculae and poorly to moderately vascularized parallel-fibered (grading locally to lamellar) periosteal cortices. Primary vasculature is predominantly longitudinal, in Proterochersis porebensis locally with radial inclination. In large specimens, secondary remodeling is significant in the deeper cortex, but it neither completely obliterates the primary tissue nor reaches the external surface of the bone in either taxon. Comparison of histological data, limb morphology, shell and limb lengths as well as proportions reveal differences in growth patterns between the taxa: Proganochelys quenstedtii seems to grow faster during early life stages than Proterochersis porebensis and attained distinctly larger body sizes earlier in ontogeny, even though the asymptotic body size is roughly the same for both species. Overall, the histological and microstructural characteristics of stylopodial bones of Triassic turtles more closely resemble those of more recent representatives of that group than earlier stem turtles.

Power investment and transmission network expansion in China and its neighbouring countries towards carbon neutrality

Transnational power cooperation contributes to promoting the large-scale use of renewable energies in Asia, but how to develop the structure of power transmission network deserves careful investigation. This paper thoroughly investigates the structure of power investment and transmission network in China and neighbouring countries, aiming to promote the large-scale use of renewable energies around Asia. Various power-generation and power-transmission technologies are considered in detail, and transnational power interconnection risk cost and carbon emission cost are embedded into a bottom-up optimization model. The future power-generation structures and networks installed under various policy scenarios are analysed in detail, and the corresponding economic, carbon emission reduction and renewable energy consumption benefits are evaluated. The results show that power transmission between China's internal regions and neighbouring countries can save $56.7 billion for the entire power system through the large-scale grid integration of renewable energy. The installed capacities of wind power, solar power, and hydropower renewable energy power generation could be increased by 141 GW, 163 GW, and 58 GW, respectively. The construction of transnational transmission lines from Central Asia to Northwest China, Mongolia to North China, the Russian Far East to Northeast China, and Northeast China to South Korea could effectively promote the utilization of renewable energy in the Russian Far East, Mongolia and Central Asia and further reduce carbon emissions in China, Japan and South Korea.