As off-road vehicles, in addition to field transportation, another vital function of agricultural tractors is to provide power for field machinery. Therefore, the dynamic performance of the power take-off (PTO) driveline directly affects the field reliability of tractors. Firstly, a torsional vibration coupled spatial dynamics model of the power take-off driveline is proposed according to the classical machine driveline dynamics and gear dynamics theory. In the dynamics model, the interactions among the vertical, lateral, and rotational motions of the driveline parts are fully included. The coupling vibrations from internal excitations (such as tooth surface friction, gear time-varying mesh, and engine pulse) and external excitations (such as field machinery load) are also considered. Secondly, the simulation results of the model are obtained using the numerical solving algorithm ode15s. The actual experiment is carried out on the indoor Tractor PTO Test Bench. Then, the model is verified by comparing the test results with the simulation results. Finally, the dynamic characteristics of the whole driveline are revealed under different drive modes, especially strong interactions between the driveline and field machinery in low-speed and heavy-load mode. The gear mesh forces and the root mean square (RMS) values of the acceleration amplitude for the main parts generally decrease gradually with the increase in the PTO rotation speed and the decrease in PTO torque. Furthermore, the model can be applied to reliability assessment, for instance, vibration, damage, and fatigue of off-road vehicles considering gear transmissions, particularly in a field working environment.