Internal Carbon Source Research Articles

Alkalinity enhanced hydrolysis of primary sludge for carbon source recovery and its impact on denitrification in wastewater treatment.

Primary sludge can serve as an internal carbon source for denitrification in wastewater treatment plants (WWTPs). This study explores the use of alkaline treatment to produce a fermentation broth from primary sludge, which predominantly contains short-chain volatile fatty acids (VFAs), with acetic acid and propionic acid making up over 65% of the total VFAs. The performance of this fermentation broth as a sole carbon source for denitrification was compared with that of sodium acetate, acetic acid, methanol, and ethanol in both biofilm and activated sludge systems. The results revealed that the denitrification rate achieved using the fermentation broth was as high as 2.1661mg NO3--N/(g MLSS·h), which was slightly lower than that of sodium acetate and acetic acid but higher than that of methanol and ethanol. The fermentation broth demonstrated a high heterotrophic yield (0.7183), an equivalent specific carbon requirement for denitrification as acetic acid and sodium acetate, and a rapid denitrification start-up. Moreover, variations in the VFAs/SCOD ratios in the fermentation broth did not significantly impact the denitrification rate or substrate biodegradation rate. However, the yield coefficient and specific carbon requirement for denitrification were found to vary significantly depending on the carbon source used. This study concludes that with appropriate treatment, fermented broth from primary sludge can be an effective carbon source comparable to commercial external carbon sources, significantly reducing carbon emissions.

The improvement on the properties and heavy metal solidification of phosphogypsum-steel slag cementitious material: Enhancement from Bi-directional carbonation

Despite the carbonation curing methods have been applied in many building materials, the low efficiency in solid waste utilization is still a big issue for applying this technique. Therefore, bi-direction carbonation curing was proposed and its performance, especially for the improvement in solidification/stabilization of heavy metal (Pb2+ and Cr3+), in phosphogypsum-steel slag cementitious materials (PSSC) was determined. In this study, PSSC with phosphogypsum (PG) and three kinds of steel slag (SS) with different fineness were prepared, and the bi-directional curing was achieved by mixing with Na2CO3 powder as the internal carbon source and curing under CO2 gas as the external carbon source. In addition to determining its effect on solidification efficiency, comprehensive strength, including X-ray diffraction, thermogravimetric analysis, and pore structure analysis were employed to reveal the changes in microstructure during carbonation after the addition of Na2CO3, and reveal the mechanism of PG on the hydration-carbonation of steel slag. The results showed the early-stage performance of PSSC was significantly improved and the solidification/stabilization performance for heavy metals was enhanced. The addition of Na2CO3 as an internal carbon source accelerates the time of carbonation curing, leading to higher early-stage strength of PSSC. By applying bi-directional carbonation curing, the solidification ability of PSSC with spatial structure denser for heavy metals Pb2+ and Cr3+ are improved by 80 % and 87 %, respectively, which could be attributed to the formation of nano-CaCO3 crystals.

Interpretable causal machine learning optimization tool for improving efficiency of internal carbon source-biological denitrification

Interpretable causal machine learning (ICML) was used to predict the performance of denitrification and clarify the relationships between influencing factors and denitrification. Multiple models were examined, and XG-Boost model provided the best prediction (R2 = 0.8743). Based on the ICML framework, hydraulic retention time (HRT), mixture chemical oxygen demand/total nitrogen (COD/TN = C/N), mixture COD concentration, and pretreatment technology were identified as important features affecting the denitrification performance. Further, tapping point and partial dependence analyses provided the range of key factors that precisely regulate denitrification. In the application analysis, HRT (6–10.5 h), mixture C/N (6–12), and mixture COD concentration (300–600 mg L−1) were the appropriate operating ranges, achieving TN removal of approximately 73 %–77 %. The effluent TN and COD concentrations met the discharge standards for wastewater in China (class 1A) and EU. These findings provide support for regulating excess sludge as internal carbon source to promote denitrification.

Efficient nitrogen and phosphorus removal performance and microbial community in a pilot-scale anaerobic/anoxic/oxic (AOA) system with long sludge retention time: Significant roles of endogenous carbon source

Stringent wastewater discharge standards require wastewater treatment plants (WWTPs) to focus on enhancing nitrogen (N) and phosphorus (P) removal efficiency. Increasing sludge concentration by regulation of sludge retention time (SRT) would enhance wastewater treatment loads. However, phosphorus-accumulating organisms (PAOs) would be outcompeted by glycogen-accumulating organisms (GAOs) under long SRT, leading to a collapse of P removal. In this work, pilot-scale anaerobic-oxic-anoxic (AOA) and anaerobic-anoxic-oxic (AAO) systems with long SRT (30 d) were parallelly established for actual urban wastewater treatment. The results indicated that sludge reflux ratio, temperature, and C/N ratio significantly impact N and P removal performance of AOA and AAO systems with long SRT, and removal efficiency of AOA system significantly exceeded that of AAO system. AOA system with long SRT achieved the optimal performance at sludge reflux ratio of 200%, temperature of 25 °C, and C/N ratio of 8, with COD, NH4+-N, TN, and PO43--P removal ratio of 92.80 ± 2.24%, 97.38 ± 0.89%, 88.97 ± 2.47%, and 94.33 ± 3.27%, respectively. In addition, compared to AAO system, AOA system could save 23.08% of the aeration volume. This work highlighted that AOA system with long SRT included multiple coupled nitrogen and phosphorus removal pathways, such as autotrophic/heterotrophic nitrification, anoxic/oxic denitrification, endogenous denitrification, and denitrifying phosphorus removal. Among these, the synergistic effect of endogenous denitrification and denitrifying phosphorus removal driven by internal carbon sources contributed to satisfactory nitrogen and phosphorus removal efficiency in AOA system with long SRT.

Enhancing energy and nitrogen removal efficiency through automatic split injection and innovative aeration device: A study of low C/N ratio environments

A new aeration device based on Bernoulli's principle, Jetventrumixer (JVM), was introduced into an aeration tank in denitrification process, which involved an automatic split injection system (ASIS) into two denitrification tanks every 10 minutes. Real-time monitoring of influent water allowed the calculation of the C/N ratio, enhancing the utilization efficiency of internal carbon sources while reducing the need for external carbon. The comparison of the JVM with the conventional air diffuser for 100 days operation showed that the removal efficiency for NH4+-N in both systems was approximately 98 %, but the nitrification efficiencies were 84 % and 80 %, respectively. This indicates that the JVM achieves an high enough removal efficiency and nitrification efficiency compared with conventional air diffuser system with dramatic reduction in energy consumption by 52.1 %. When the influent wastewater was split and injected into duplicate denitrification tanks at ratios of 3:7, 5:5, and 7:3, the total nitrogen (TN) removal efficiencies were 77 %, 73 %, and 72 %, respectively. In contrast, with the implementation of the ASIS, the TN removal efficiency increased up to 82 %. The increase in TN removal indicates that real-time monitoring could stably track changes chemical composition in wastewater influent over 24 h and introducing ASIS facilitate the efficient utilization of internal carbon sources, thereby enhancing denitrification efficiency and improving TN removal efficiency. Finally, the greenhouse gas (GHG) emissions from the JVM and air diffuser were 9.39401 and 19.60488 tCO2eq year-1, respectively, representing a 52% reduction. Therefore, JVM and ASIS successfully reduced energy consumption and enhanced both nitrification and denitrification efficiencies.

Molecular evidence of internal carbon-driven partial denitrification in a mainstream pilot A-B system coupled with side-stream EBPR treating municipal wastewater

Achieving mainstream short-cut nitrogen removal via nitrite has become a carbon and energy efficient way, but still remains challenging for low-strength municipal wastewaters. This study integrated sidestream enhanced biological phosphorus removal system in a pilot-scale adsorption/bio-oxidation (A-B) process (named A-B-S2EBPR system) and nitrite accumulation was successfully achieved for treating the municipal wastewater. Nitrite could accumulate to 5.5 ± 0.3 mg N/L in the intermittently aerated tanks of B-stage with the nitrite accumulation ratio (NAR) of 79.1 ± 6.5 %. The final effluent concentration and removal efficiency of total inorganic nitrogen (TIN) were 4.6 ± 1.8 mg N/L and 84.9 ± 5.6 %, respectively. In-situ process performance of nitrogen conversions, routine batch nitrification/denitrification activity tests and functional gene abundance of nitrifiers collectively suggested that the nitrite accumulation was mainly caused by partial denitrification rather than out-selection of nitrite oxidizing bacteria (NOB). Moreover, the single-cell Raman spectroscopy analysis first demonstrated that there was a specific microbial population that could utilize polyhydroxyalkanoates (PHA) as the potential internal carbon source during the partial denitrification process. The integration of S2EBPR brings unique features to the conventional A-B process, such as extended anaerobic retention time, lower oxidation–reduction potential (ORP), much higher and complex volatile fatty acids (VFAs) etc., which can largely reshape the microbial communities. The dominant genera were Acinetobacter and Comamonadaceae, which accounted for (17.8 ± 15.5)% and (6.7 ± 3.4)%, respectively, while the relative abundance of conventional nitrifiers was less than 0.2%. This study provides insights into phylogenetic and phenotypic shifts of microbial communities when incorporating S2EBPR into the sustainable A-B process to achieve mainstream short-cut nitrogen removal.

Enhanced wastewater treatment with an AnF-AAO system for improved internal carbon source utilization

The main challenge in removing nutrients from municipal wastewater in China is the lack of available carbon sources. While hydrolysis acidification tanks can improve wastewater biodegradability by effectively utilizing internal carbon sources, high sludge concentrations are difficult to control in traditional tank variants. In this study, an innovative anaerobic filter (AnF) hydrolysis acidification reactor composed of a continuously stirred tank reactor (CSTR) and cloth media filter was designed to regulate and maintain high sludge concentrations in the hydrolysis acidifier. The reactor was used as a pretreatment unit for the anaerobic/anoxic/oxic (AAO) units and combined into an AnF-AAO system to explore the effectiveness of internal carbon source utilization in wastewater. The results indicate that as the sludge concentration in the hydrolysis acidifier increased, the hydrolysis and acidification processes became more efficient. The optimal sludge concentration was 40 g/L, which significantly increased the production of soluble chemical oxygen demand and volatile fatty acids. Above this concentration, the efficiency decreased. Compared to traditional AAO processes, the AnF-AAO system achieved superior total nitrogen and phosphorus removal with shorter hydraulic retention times and reduced sludge production by a significant amount of 35%. Due to its capacity for enhancing internal carbon source utilization, the AnF-AAO system constitutes a promising approach for sustainable urban wastewater treatment.

Partial denitrification in rope-type biofilm reactors: Performance, kinetics, and microflora using internal vs. external carbon sources

Stable nitrite accumulation through partial denitrification (PDN) represents an efficient pathway to support the anammox process, but limited studies explored the internal wastewater carbon sources and biofilm processes. This study assessed the viability of the PDN process, biofilm community evolution, and functional enzyme formation in rope-type biofilm media reactors using primary effluent (PE) and anaerobically pretreated wastewater carbon sources for the first time. Comparison was made with external carbon (acetate) under varied pH and biofilm thicknesses, maintaining a favourable sCOD: NO3-N ratio of 3. The wastewater’s internal carbon resulted in thinner biofilms; nevertheless, modest nitrite accumulation (0.24 g/m2/d) occurred only at elevated pH. The highest nitrite accumulation (0.79 g/m2/d) was exhibited in the biofilm thickness-controlled acetate-fed reactor, featuring porous biofilms dominated by denitrifier Thauera (10.24 %) and imbalance between Nar, Nap, and Nir reductases. Using internal wastewater carbon sources offers a sustainable avenue for adopting the PDN process in full-scale application.

Dual-Carbon Phase-Encapsulated Prelithiated SiOx Microrod Anode for Lithium-Ion Batteries.

Among silicon-based anode family for Li-ion battery technology, SiOx, a nonstoichiometric silicon suboxide holds the potential for significant near-term commercial impact. In this context, this study mainly focuses on demonstrating an innovative SiOx@C anode design that adopts a pre-lithiation strategy based on in situ pyrolysis of Li-salt of silsesquioxane trisilanolate without the need for lithium metal or active lithium compounds and creates dual carbon encapsulation of SiOC nanodomains by simply one-step thermal treatment. This ingenious design ensures the pre-lithiation process and pre-lithiation material with high-environmental stability. Moreover, phenyl-rich organosiloxane clusters and polyacrylonitrile polymers are expected to serve as internal and external carbon source, respectively. The formation of an interpenetrating and continuous carbon matrix network would not only synergistically offer an improved electrochemical accessibility of active sites but also alleviate the volume expansion effect during cycling. As a result, this new type of anode delivered a high reversible capacity, remarkable cycle stability as well as excellent high-rate capability. In particular, the L2-SiOx@C material has a high initial coulomb efficiencof 80.4% and, after 500 cycles, a capacity retention as high as 97.5% at 0.5 A g-1 with a reversible specific capacity of 654.5mA h g-1.

Influence of hydraulic retention time on advanced synergistic nitrogen removal using internal carbon source

Hydraulic retention time (HRT) is a significant operational eter of denitrifying filter (DF). The influence of HRT on the performance and mechanism of advanced synergistic nitrogen removal (ASNR) of partial-denitrification anammox (PDA) and denitrification was investigated, consuming the hydrolytic and oxidation products of refractory organics in the secondary effluent (SE) as carbon source. When the HRT was 8, 6, 4 and 2 h, the filtered effluent total nitrogen (TN) was 1.47, 3.47, 4.17 and 4.49 mg/L, and the effluent CODcr was 8.12, 9.95, 9.40 and 9.87 mg/L, respectively. Removing 1 mg of TN actually consumed 0.87–0.96 mg CODcr, and the contribution rate of PDA to TN removal was 62.10–70.02 %. A variety of manganese oxidizing (MnOB), hydrolytic, anammox and denitrifying bacteria were identified, and their abundance was obviously affected by the HRT. The lack of carbon source was the main factor limiting the ASNR efficiency, rather than the HRT.

Insightful Analysis and Prediction of SCOD Component Variation in Low-Carbon/Nitrogen-Ratio Domestic Wastewater via Machine Learning

The rapid identification of the amount and characteristics of chemical oxygen demand (COD) in influent water is critical to the operation of wastewater treatment plants (WWTPs), especially for WWTPs in the face of influent water with a low carbon/nitrogen (C/N) ratio. Given that, this study carried out batch kinetic experiments for soluble chemical oxygen demand (SCOD) and nitrogen degradation for three WWTPs and established machine learning (ML) models for the accurate prediction of the variation in SCOD. The results indicate that four different kinds of components were identified via parallel factor (PARAFAC) analysis. C1 (Ex/Em = 235 nm and 275/348 nm, tryptophan-like substances/soluble microbial by-products) contributes to the majority of internal carbon sources for endogenous denitrification, whereas C4 (230 nm and 275/350 nm, tyrosine-like substances) is crucial for readily biodegradable SCOD composition according to the machine learning (ML) models. Furthermore, the gradient boosting decision tree (GBDT) algorithm achieved higher interpretability and generalizability in describing the relationship between SCOD and carbon source components, with an R2 reaching 0.772. A Shapley additive explanations (SHAP) analysis of GBDT models further validated the above result. Undoubtedly, this study provided novel insights into utilizing ML models to predict SCOD through the measurements of the excitation–emission matrix (EEM) in specific Ex and Em positions. The results could help us to identify the degradation and transformation relationship between different kinds of carbon sources and nitrogen species in the wastewater treatment process, and thus provide a novel guidance for the optimized operation of WWTPs.

Targeting Mycobacterium tuberculosis Persistence through Inhibition of the Trehalose Catalytic Shift.

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), is the leading cause of death worldwide by infectious disease. Treatment of Mtb infection requires a six-month course of multiple antibiotics, an extremely challenging regimen necessitated by Mtb's ability to form drug-tolerant persister cells. Mtb persister formation is dependent on the trehalose catalytic shift, a stress-responsive metabolic remodeling mechanism in which the disaccharide trehalose is liberated from cell surface glycolipids and repurposed as an internal carbon source to meet energy and redox demands. Here, using a biofilm-persister model, metabolomics, and cryo-electron microscopy (EM), we found that azidodeoxy- and aminodeoxy-d-trehalose analogues block the Mtb trehalose catalytic shift through inhibition of trehalose synthase TreS (Rv0126), which catalyzes the isomerization of trehalose to maltose. Out of a focused eight-member compound panel constructed by chemoenzymatic synthesis, the natural product 2-trehalosamine exhibited the highest potency and significantly potentiated first- and second-line TB drugs in broth culture and macrophage infection assays. We also report the first structure of TreS bound to a substrate analogue inhibitor, obtained via cryo-EM, which revealed conformational changes likely essential for catalysis and inhibitor binding that can potentially be exploited for future therapeutic development. Our results demonstrate that inhibition of the trehalose catalytic shift is a viable strategy to target Mtb persisters and advance trehalose analogues as tools and potential adjunctive therapeutics for investigating and targeting mycobacterial persistence.

First application of the novel anaerobic/aerobic/anoxic (AOA) process for advanced nutrient removal in a wastewater treatment plant

The stringent effluent quality standards in wastewater treatment plants (WWTPs) can effectively mitigate environmental issues such as eutrophication by reducing the discharge of nutrients into water environments. However, the current wastewater treatment process often struggles to achieve advanced nutrient removal while also saving energy and reducing carbon consumption. The first full-scale anaerobic/aerobic/anoxic (AOA) system was established with a wastewater treatment scale of 40,000 m3/d. Over one year of operation, the average TN and TP concentration in the effluent of 7.53 ± 0.81 and 0.37 ± 0.05 mg/L was achieved in low TN/COD (C/N) ratio (average 5) wastewater treatment. The post-anoxic zones fully utilized the internal carbon source stored in pre-anaerobic zones, removing 41.29 % of TN and 36.25 % of TP. Intracellular glycogen (Gly) and proteins in extracellular polymeric substances (EPS) served as potential drivers for post-anoxic denitrification and phosphorus uptake. The sludge fermentation process was enhanced by the long anoxic hydraulic retention time (HRT) of the AOA system. The relative abundance of fermentative bacteria was 31.66 - 55.83 %, and their fermentation metabolites can provide additional substrates and energy for nutrient removal. The development and utilization of internal carbon sources in the AOA system benefited from reducing excess sludge production, energy conservation, and advanced nutrient removal under carbon-limited. The successful full-scale validation of the AOA process provided a potentially transformative technology with wide applicability to WWTPs.

Study on culture of stable aerobic granular sludge

In this study, three reactors were established with anaerobic plug-flow times of 1.5 hours, 1 hour, and 0.5 hours. The results indicated that the anaerobic plug-flow time of 1 hour constructed favorable "feast- famine" period ratio, promoting the enrichment of microorganisms that stored and utilized the internal carbon source, such as Phosphate and Glycogen Accumulating Organisms (PAO and GAO). This resulted in the formation of granular sludge with both short granulation time and structural stability. In contrast, shortening the anaerobic feed time to 0.5 hours was detrimental to the growth of GAO, leading to slow granularization of aerobic sludge. The anaerobic influent time of 1.5 h leads to the shortening of starvation period and poor stability of particle structure.

Enhancing nitrogen removal performance through intermittent aeration in continuous plug-flow anaerobic/aerobic/anoxic process treating low-strength municipal sewage

Advanced nitrogen removal cannot be achieved through the conventional biological nitrogen removal process, which requires higher carbon sources and aeration energy. The proposal of intermittent aeration in the aerobic chambers offered an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio (C/N) municipal sewage, using a plug-flow reactor with anaerobic/aerobic/anoxic (AOA) process. Due to the effective utilization of internal carbon sources through the intermittent aeration, the total inorganic nitrogen removal efficiency (NRE) increased to 77.9 ± 3.2 % with the mean aerobic hydraulic retention time of only 3.2 h and a low C/N of 3.3 during the operation of 210 days. Polyhydroxyalkanoates dominated the nitrogen removal in this AOA system, accounting for 48.0 %, primarily occurring in the alternant aerobic/anoxic chambers. Moreover, the microbial community structure remained unchanged while the NRE increased to 77.9 %. This study provided an efficient and economic strategy for the continuous plug-flow AOA process.

The distribution of CO2 on Europa indicates an internal source of carbon.

Jupiter's moon Europa has a subsurface ocean, the chemistry of which is largely unknown. Carbon dioxide (CO2) has previously been detected on the surface of Europa, but it was not possible to determine whether it originated from subsurface ocean chemistry, was delivered by impacts, or was produced on the surface by radiation processing of impact-delivered material. We mapped the distribution of CO2 on Europa using observations obtained with the James Webb Space Telescope (JWST). We found a concentration of CO2 within Tara Regio, a recently resurfaced terrain. This indicates that the CO2 is derived from an internal carbon source. We propose that the CO2 formed in the internal ocean, although we cannot rule out formation on the surface through radiolytic conversion of ocean-derived organics or carbonates.

Evaluating the Effect of Nonaerobic Hydraulic Retention Time on Nutrients Removal in Nonaerobic and Aerobic SBR

The nonaerobic stage is critical for the processes of denitrification and phosphorus release in biological wastewater treatment. A lab-scale sequencing batch reactor (SBR) operating as nonaerobic/aerobic was used to treat synthetic wastewater, and the nutrients removal performance was investigated under different nonaerobic hydraulic retention times (HRTs). The results showed when the nonaerobic HRT changed from 0 to 4 h with constant aerobic HRT of 8 h [dissolved oxygen (DO) 0.50–1.50 mg·L−1], the removal efficiencies of PO43−─P and NH4+─N were all above 90.00%, respectively. The nonaerobic HRT could influence carbon utilization between denitrification and phosphorus release, which showed a close relationship with the ratio of consumed chemical oxygen demand (COD)phosphorus release to CODdenitrification (0.94). Specifically, the consumed CODphosphorus release increased with the increase of nonaerobic HRT (0.90), whereas the relationship between consumed CODdenitrification and nonaerobic HRT was limited (−0.60). Furthermore, internal carbon source produced through phosphorus release could promote simultaneous nitrification denitrification. This control strategy could be conveniently applied in intermittent-flow sewage treatment plant through adjusting nonaerobic HRT.

Efficient nitrogen removal in a total floc sludge system from domestic wastewater with low C/N: High anammox nitrogen removal contribution driven by endogenous partial denitrification

Since unsustainable partial nitrification prone to unstable nitrogen removal rates, cultivation and enrichment of AnAOB, further improve autotrophic nitrogen removal contribution have been challenges in the mainstream anammox process. This study proposed a new strategy to enrich AnAOB motivated by endogenous partial denitrification (EPD) in total floc sludge system through the AOA process with sustainable nitrification. The results showed that in the presence of NH4+ and NO3– at the anoxic stage of N-EPDA, Ca. Brocadia was enriched (0.005%→0.92%) in floc sludge via internal carbon source metabolism of EPD. The C/N and temperature of N-EPDA were also optimized to achieve higher activities of EPD and anammox. The N-EPDA was operated at low C/N ratio (3.1) with anammox nitrogen removal contribution of 78% during the anoxic stage, Eff.TIN of 8.3 mg/L and NRE of 83.5% during phase III, achieved efficient autotrophic nitrogen removal and AnAOB enrichment in the absence of partial nitrification.

In-situ sulfite treatment enhanced the production of short-chain fatty acids from waste activated sludge in the side-stream anaerobic fermentation

Sulfite-based technology could enhance methane production from anaerobic sludge digestion. However, its potential for in-situ direct sludge treatment without anaerobic sludge addition in the side-stream remains unclear. This study investigated the feasibility of using in-situ sulfite treating sludge for short-chain fatty acids (SCFAs) production via anaerobic fermentation of waste activated sludge (WAS) as a side-stream treatment. In-situ sulfite direct sludge treatment enhanced SCFAs and acetic acid production by 2.03 and 4.89 times at 500 mg S/L compared to the control. With in-situ sulfite treatment, WAS hydrolysis and acidification were enhanced while methanogenesis was spontaneously hindered. The in-situ sulfite treatment inactivated pathogens and improved the sludge dewatering properties. The relative abundances of SCFAs-production microbial were stimulated, facilitating the sludge bioconversion. The produced SCFAs from in-situ sulfite side-stream treatment could be applied as an “internal carbon source” to enhance biological nutrient removal to improve economic and environmental value from sludge treatment.

Preparation of LiFePO4 composite based on dual carbon sources of phytic acid and glucose and its performance for lithium extraction from salt lake

In this study, biomass phytic acid (PhyA) was used as phosphorus source and internal carbon source, and glucose (GC) was used as external carbon source to prepare dual carbon source LiFePO4 (GC/IC/LFP) electrode material containing intercalated carbon (IC) and coated carbon (GC). Based on the working principle of rocking chair lithium ion battery, a new electrochemical system was constructed to efficiently extract Li+ from salt lake brine with high Mg/Li ratio (53) and Na/Li ratio (416). The effects of electrolytic voltage and coexisting ions on the capacity of lithium extraction/insertion at room temperature were investigated. It was found that when the electrolytic voltage was 1.0 V, the insertion capacity of lithium in pure lithium solution could reach 43.67 mg·LiFePO4 (1 g)−1, which was 99.25 % of the theoretical capacity (44 mg). The lithium extraction capacity can reach 43.20 mg·LiFePO4 (1 g)−1, which is 98.92 % of the insertion capacity. In the simulated salt lake brine with a large number of coexisting ions, when the electrolytic voltage is 0.8 V, the lithium insertion capacity can reach 38.09 mg·LiFePO4 (1 g)−1, which is 86.57 % of the theoretical capacity, and the lithium extraction capacity can reach 35.00 mg·LiFePO4 (1 g)−1, which is 91.89 % of the insertion capacity. The system maintains high selectivity for Li+ extraction. The concentration of coexisting ions of K+, Mg2+, Na+ and Ca2+ decreased by more than 8800, 4500, 25,000 and 1200 times, respectively. The Mg/Li ratio decreased from 53 to 0.09, and the Na/Li ratio decreased from 416 to 0.10. After ten times of extraction, the electrode still has high coexisting Men+/Li+ separation performance, although the extraction performance is decreased to a certain extent.