Internal Architecture Research Articles

Three-dimensional seismic evidence for depositional undulations nucleated around pre-existing relief, South China Sea

Sediment undulations are widely distributed along continental shelf and slope worldwide. The driving mechanisms of undulations are crucial for assessing the long-term stability of continental slope, which, however, remain poorly understood. We used high-resolution 3D seismic data to investigate the internal architecture and origin of a field of seafloor and subsurface undulations along the slope of South China Sea (SCS). The undulations have narrow troughs and broad ridges. The reflection geometries in the troughs between undulations tend to be continuous and not offset by faults. These troughs terminate at different subsurface depths, commonly ranging from 50 to 400 ms in two-way travel time (TWTT) below the seafloor, often at locations related to subsurface geological structures, such as buried faults and steps within mass transport deposits (MTDs). These subsurface terminations therefore suggest that the undulations are probably depositional features not linked to creep-related basal detachment. The troughs between undulations display an absence of growth features, while the undulations increase in amplitude from base to top (characteristics that are also incompatible with gravitational deformation). Erosional and depositional features are commonly found in the undulations; thus, we interpret the narrow troughs between undulations to represent the areas where sediment-laden bottom currents undergo passage from supercritical to subcritical conditions. These troughs nucleated mostly from fault scarps and seafloor steps induced by past slope failures. Previous alternative interpretations report that these features originate from subsurface creep; alternatively, the present interpretation as sediment transport structures has significant implications for seafloor management and engineering projects.

Intrinsic and Dynamic Heterogeneity of Nonlamellar Lyotropic Liquid Crystalline Nanodispersions.

Nonlamellar lyotropic liquid crystalline (LLC) nanoparticles are a family of versatile nano-self-assemblies, which are finding increasing applications in drug solubilization and targeted drug delivery. LLC nanodispersions are heterogeneous with discrete nanoparticle subpopulations of distinct internal architecture and morphology, frequently coexisting with micelles and/or vesicles. Diversity in the internal architectural repertoire of LLC nanodispersions grants versatility in drug solubilization, encapsulation, and release rate. However, drug incorporation contributes to the heterogeneity of LLC nanodispersions, and on exposure to biological media, LLC nanodispersions often undergo nanostructural and morphological transformations. From a pharmaceutical perspective, coexistence of multiple types of nanoparticles with diverse structural attributes, together with media-driven transformations in colloidal characteristics, brings challenges in dissecting biological and therapeutic performance of LLC nanodispersions in a spatiotemporal manner. Here, we outline innate and acquired heterogeneity of LLC nanodispersions and discuss technological developments and alternative approaches needed to improve homogeneity of LLC formulations for drug delivery applications.

3D morphometry of De Geer Moraines and Crevasse-Squeeze Ridges: Differentiating between pushing and squeezing mechanisms from remotely sensed data

De Geer Moraines (DGM) and Crevasse-Squeeze Ridges (CSR) are important landforms that can provide useful insights regarding palaeo-glacial processes. Specifically, these landforms can provide information concerning ice-marginal dynamics, and/or subglacial processes, depending on the context in which they are formed. The extraction of 3D morphometric data from these ridges can help to elucidate their formational processes, and potentially enable landform differentiation. We develop a new Python-based ArcGIS toolbox that can automatically extract 3D morphometric data from large sample sets of linear features. The morphometry toolbox may be applied to a wide range of research disciplines that are concerned with quantifying the morphometry of any elongated landforms. This is particularly useful for DGM and CSR studies, where visual similarities can result in confusion over landform type and/or formation. Here we present a case study from southwest Finland and the Northwest Territories, Canada, whereby high-resolution 3D morphometric data is used to analyse and classify DGMs and CSRs. The results reveal key differences in morphometric properties between the landforms which enables a quantified foundation by which to differentiate them. The studied CSRs are found to be higher, wider, steeper, more symmetrical, less sinuous and more voluminous than the studied prominent DGM. In contrast, a tendency for cross-sectional asymmetry in DGM supports an origin by ice-marginal pushing, rather than basal squeeze-up into crevasses. This is further supported by CSRs being less sinuous than DGM due to them being constrained to the dimensions and planform of the (relatively straight) host crevasses, whereas DGM follow a more sinuous path related to the ice margin shape. Future work should include sedimentological and geophysical studies to constrain DGM internal architecture and formation processes. The results may then be used to validate the application of DGM for detailed ice marginal reconstructions.

Effect of atorvastatin on muscle tissues of dermatomyositis and antisynthetase syndrome patients with dyslipidemia.

In a recent study, we have shown that atorvastatin is clinically safe for dermatomyositis (DM) and antisynthetase syndrome (ASS) patients with dyslipidemia. Herein, we showed in an unprecedented way, the safety of atorvastatin on the muscular tissues of these patients. Transcriptome analysis was performed on samples of the vastus lateralis muscle obtained at baseline and after 12 weeks of atorvastatin (20 mg/day) intervention in DM or ASS patients with dyslipidemia [6DM and 5ASS received atorvastatin, and 2DM and 3ASS received placebo]. The results were analyzed considering differences in expression fold change before and after treatment. Histological and histochemical analyses were also performed. In both groups, no significant changes were observed in genes related to the mitochondrial, oxidative, insulin, lipid, and fibrogenic pathways. Histological analysis showed a slight variability in the fiber size that was preserved after the intervention. In addition, the mosaic of muscle fibers was preserved in the internal architecture of the fibers and all histological regions. No fiber necrosis or atrophy, focal failures, subsarcolemmal accumulation, lipids, areas of fibrosis, or alterations in mitochondrial activity were observed. All muscle fibers were labeled for MHC I. Atorvastatin did not promote significant changes in the expression of genes related to mitochondrial, oxidative, insulin, lipid, and fibrogenic pathways in the muscle tissues of DM and ASS patients with dyslipidemia. Atorvastatin did not also promote histological and histochemical changes in muscle tissues. Our results reinforce the safety of the administration of atorvastatin to treat dyslipidemia in patients with DM and ASS.

Investigating the Architecture and Characteristics of Asian Hornet Nests: A Biomimetics Examination of Structure and Materials.

This study investigates the internal architecture of Asian hornet nests (AHNs) using advanced imaging techniques, such as CT scanning and X-ray radiography, to understand their construction and function. The primary objective and significance of this study centre on drawing inspiration from the creative way Asian hornets construct their nests, with a particular focus on the architecture, design, functionality, and building materials of these nests. The architectural principles governing the construction of these nests, such as the arrangement of hexagonal cells, pedicels for load bearing, and adhesive materials, serve as a source of inspiration for innovative and sustainable design practices. The pedicels in Asian hornet nests play a crucial role in transferring load and ensuring stability. Additionally, AHNs' adhesion to tree branches is essential for preventing collapse, and the pedicels provide necessary structural support. The knowledge gained from studying AHNs' internal architecture could be applied directly to the architecture and civil engineering fields to improve structure stability and durability. The microstructure analysis of the paper-like material that hornets produce to build their nests indicates a complex and heterogeneous structure, composed of various plant fragments and fibres. This unique composition creates intricate grooves and pores, which are essential for regulating temperature and humidity levels within the outer envelope of the nest. The study of Asian hornet nests' internal structure demonstrated that nature's engineering principles inspire the design of durable and resilient structures in the construction industry. Civil engineers can incorporate similar principles into their designs to enhance the structural integrity and performance of buildings, bridges, and other infrastructure.

Metamaterial design and continuously adjustable mechanical properties based on novel planetary gear trains

Mechanical metamaterials have been pursuing as valuable properties attribute to the unique internal architecture, but are generally limited to the predetermined properties after manufacturing, resulting in the inability to continuously change the properties during application. Aiming for the continuously adjustable property under environments with high reliability requirements, this research developed two types of mechanical metamaterials based on planetary gear train. The principle of continuously adjustable mechanical property and dynamic characteristics of metamaterial based on planetary gear train is researched though a theoretical model of curved beam under varying radial load. Further, mechanical metamaterials based on double-layers planetary gear (DLPG) train and Ravigneaux planetary gear (RPG) train are designed. The compressive elastic modulus, shear modulus, and amplitude-frequency response are calculated and investigated with finite element simulation. The results, with an adjustable range respectively within 9–15 times and 2–3 times in compressive elastic modulus of and shear modulus, show a good agreement between the theoretical and simulated results, and illustrate the adjustability and reliability of the mechanical metamaterials based on planetary gear train. Finally, the potential configurations and applications of metamaterial based on planetary gear train are presented.

Influence of Internal Microarchitecture on the Shape of Individual Implants Made from Vinylidene Fluoride Copolymer by 3D Printing with High-Temperature Crystallization

The healing potential of individual polymer implants for the reconstruction of extensive craniofacialdefects after cancer resection is largely determined by the internal architecture of the implant. The architectureof an implant during polymer crystallization could affect the structure and shape of the implant at themicro and macro levels. In this study, the relationship between the internal architecture (triply periodic minimumsurface structure (gyroid), cube, grid, and honeycomb) and shape changes of individual implants by3D printing with a vinylidene fluoride-tetrafluoroethylene copolymer after crystallization is examined at afilling density of 70%. Using the method of differential scanning calorimetry, it is established that crystallizationleads to the rearrangement of the crystalline structure of the implant into electrically active (ferroelectric)crystalline phases. Moreover, the type of internal architecture affects the change in the shape of theimplant after crystallization. The results of the computed tomography show that structures with a triply periodicminimum surface (gyroid) provide the minimal deformation of the implant during crystallization, whichmakes such structures optimal for manufacturing implants for replacing bone defects in the zygomatic-orbitalcomplex.

30 Hippocampal Internal Architecture Subfield Volumes Associated with Systematic Inflammatory Biomarkers in Multiple Sclerosis

Objective:Multiple Sclerosis (MS) affects up to 500,000 adults in the United States, with cognitive impairment present in 45%-65% of people. Studies showed hippocampal atrophy in MS, but the underlying mechanisms remain unknown. Inflammation has been proposed to play a significant role, and associations between systemic inflammatory biomarkers and hippocampal atrophy have been shown in other neurological conditions. However, research exploring serum biomarker and volumetric associations in MS are lacking. Given that conventional imaging methods lack resolution for hippocampal internal architecture (HIA), new protocols were developed. We used the High-Resolution Multiple Image Co-Registration and Averaging (HR-MICRA) method to visualize the HIA subfields. We investigated the relationship between subfield volumes generated from HR-MICRA scans and systemic serum biomarkers in MS.Participants and Methods:Patients with MS were recruited (N= 34, mean age= 54.6, 35.3% Black) underwent Magnetic Resonance Imaging (MRI), and serum biomarkers were obtained, specifically chosen for their potential role in MS. Inflammatory biomarkers included; granulocyte colony stimulating factor (G-CSF), interleukin-10 (IL-10), matrix metalloproteinase-9 (MMP-9), tumor necrosis factor- a (TNF- a), and growth factors; vascular endothelial growth factor (VEGF); insulin-like growth factor-1 (IGF-1), and brain derived growth factor (BDNF). Imaging was performed in a Siemens Prisma 3T scanner with a 64-channel head coil using the HR-MICRA method. Hippocampal subfields were calculated using the Automated Segmentation of Hippocampal Subfields (ASHS) package. We used the Magdeburg Young Adult 7T Atlas for sub-hippocampal structures and Penn Temporal Lobe Epilepsy T1-MRI Whole Hippocampus ASHS Atlas for general hippocampal structure and segmentation. Pearson's product-moment analyses provided correlations between biomarkers and hippocampal subfield volumes for each cerebral hemisphere. A statistical significance level of p < 0.05 was used for all analyses.Results:Correlations emerged between left hemisphere Cornu Ammonis (CA) 2 and G-CSF (r = -.384; p = .025); IL-10 (r = -.342; p = .048); VEGF (r = -.371; p= .031); and CA3 with IL-10 (r = -.488, p = .003); G-CSF (r = -.386; p= .024); VEGF (r = -.352; p= .041). Dentate gyrus correlated with MMP-9 (r =.416; p=.014); IL-10 (r = -.365; p =.034). BDNF was correlated with right hemisphere CA1 (r = -.417, p = .014), CA2 (r = -.497; p= .003) and CA3 (r = -.451; p=.007).Conclusions:In our sample of persons with MS, left hemisphere hippocampal subfield volumes were negatively correlated with inflammatory biomarkers, supporting previous reports linking inflammation to reduced brain volumes in other neurological conditions. In the right hemisphere, we found negative correlations between HIA and BDNF, suggesting a neuroprotective function for BDNF in this neurodegenerative disease. These findings in a representative sample of patients with MS highlight the need for further research exploring the relationship between HIA and systemic serum biomarkers in MS.

Effect of 3 Weeks of Strict Head‑Down Tilt Bed Rest on Human Muscle Fuction and Architecture

The aim of this study was to first, experimentally measure in vivo changes in the length, fiber angle and thickness of the medial gastrocnemius muscle (MG) in young men in response to changes in foot position and joint moment during isometric plantar flexion and, in secondly, to compare the changes in the above characteristics of the muscular architecture that occur during the transition from a state of rest to a given isometric intensity during plantar flexion. The internal architecture of the MG was determined after 21-day of strict head‑down tilt bed rest (HDT). MG scanning was performed using ultrasound at rest at ankle joint angles of –15° (dorsiflexion), 0° (neutral), +15° and +30° (plantar flexion). Additional ultrasounds were performed during maximal voluntary contraction (MVC) and additionally at 80, 60, 40, and 20% of the MVC with the ankle in neutral position. In each position, longitudinal ultrasound images of the MG were obtained in a relaxed (passive) state with the determination of the length (Lf) and angles of fascicles (Θf) relative to the aponeurosis. After HDT, the thickness of the MG during graduated isometric force up to 80% of the MVC in the neutral position of the ankle joint remained constant. Various Lf and Θf and their changes after HDT can be a limiting factor in the generation of muscle contractile functions. The results of the study show that the change in muscle structure during contraction compared to rest, as measured by changes in muscle architecture, can be used to assess muscle mechanical output.

Multilayer Hydrogel Microcubes: Effects of Templating Particle Morphology on Cubic Hydrogel Properties

AbstractNon‐spherical stimuli‐responsive polymeric particles have shown critical importance in therapeutic delivery. Herein, pH‐responsive poly(methacrylic acid) (PMAA) cubic hydrogel microparticles are synthesized by crosslinking PMAA layers within PMAA/poly(N‐vinylpyrrolidone) hydrogen‐bonded multilayers templated on porous inorganic microparticles. This study investigates the effects of template porosity and surface morphology on the PMAA multilayer hydrogel microcube properties. It is found that the hydrogel structure depends on the template's calcination time and temperature. The pH‐triggered PMAA hydrogel cube swelling depends on the hydrogel's internal architecture, either hollow capsule‐like or non‐hollow continuous hydrogels. The loading efficiency of the doxorubicin (DOX) drug inside the microcubes is analyzed by high‐performance liquid chromatography (HPLC), and shows the dependenceof the drug uptake on the network structure and morphology controlled by the template porosity. Varying the template calcination from low (300 °C) to high (1000 °C) temperature results in a 2.5‐fold greater DOX encapsulation by the hydrogel cubes. The effects of hydrogel surface charge on the DOX loading and release are also studied using zeta‐potential measurements. This work provides insight into the effect of structural composition, network morphology, and pH‐induced swelling of the cubical hydrogels and may advance the development of stimuli‐responsive vehicles for targeted anticancer drug delivery.

Interactions between interfaces dictate stimuli-responsive emulsion behaviour

Stimuli-responsive emulsions offer a dual advantage, combining long-term storage with controlled release triggered by external cues such as pH or temperature changes. This study establishes that thermo-responsive emulsion behaviour is primarily determined by interactions between, rather than within, interfaces. Consequently, the stability of these emulsions is intricately tied to the nature of the stabilizing microgel particles - whether they are more polymeric or colloidal, and the morphology they assume at the liquid interface. The colloidal properties of the microgels provide the foundation for the long-term stability of Pickering emulsions. However, limited deformability can lead to non-responsive emulsions. Conversely, the polymeric properties of the microgels enable them to spread and flatten at the liquid interface, enabling stimuli-responsive behaviour. Furthermore, microgels shared between two emulsion droplets in flocculated emulsions facilitate stimuli-responsiveness, regardless of their internal architecture. This underscores the pivotal role of microgel morphology and the forces they exert on liquid interfaces in the control and design of stimuli-responsive emulsions and interfaces.

An imaging review of the hippocampus and its common pathologies.

The hippocampus is a complex structure located in the mesial temporal lobe that plays a critical role in cognitive and memory-related processes. The hippocampal formation consists of the dentate gyrus, hippocampus proper, and subiculum, and its importance in the neural circuitry makes it a key anatomic structure to evaluate in neuroimaging studies. Advancements in imaging techniques now allow detailed assessment of hippocampus internal architecture and signal features that has improved identification and characterization of hippocampal abnormalities. This review aims to summarize the neuroimaging features of the hippocampus and its common pathologies. It provides an overview of the hippocampal anatomy on magnetic resonance imaging and discusses how various imaging techniques can be used to assess the hippocampus. The review explores neuroimaging findings related to hippocampal variants (incomplete hippocampal inversion, sulcal remnant and choroidal fissure cysts), and pathologies of neoplastic (astrocytoma and glioma, ganglioglioma, dysembryoplastic neuroepithelial tumor, multinodular and vacuolating neuronal tumor, and metastasis), epileptic (mesial temporal sclerosis and focal cortical dysplasia), neurodegenerative (Alzheimer's disease, progressive primary aphasia, and frontotemporal dementia), infectious (Herpes simplex virus and limbic encephalitis), vascular (ischemic stroke, arteriovenous malformation, and cerebral cavernous malformations), and toxic-metabolic (transient global amnesia and opioid-associated amnestic syndrome) etiologies.

Biofilms as self-shaping growing nematics.

Active nematics are the nonequilibrium analogue of passive liquid crystals. They consist of anisotropic units that consume free energy to drive emergent behaviour. Like liquid crystal molecules in displays, ordering and dynamics in active nematics are sensitive to boundary conditions. However, unlike passive liquid crystals, active nematics have the potential to regulate their boundaries through self-generated stresses. Here, we show how a three-dimensional, living nematic can actively shape itself and its boundary to regulate its internal architecture through growth-induced stresses, using bacterial biofilms confined by a hydrogel as a model system. We show that biofilms exhibit a sharp transition in shape from domes to lenses upon changing environmental stiffness or cell-substrate friction, which is explained by a theoretical model that considers the competition between confinement and interfacial forces. The growth mode defines the progression of the boundary, which in turn determines the trajectories and spatial distribution of cell lineages. We further demonstrate that the evolving boundary and corresponding stress anisotropy define the orientational ordering of cells and the emergence of topological defects in the biofilm interior. Our findings may provide strategies for the development of programmed microbial consortia with emergent material properties.

Topological damping in an ultrafast giant cell

Cellular systems are known to exhibit some of the fastest movements in biology, but little is known as to how single cells can dissipate this energy rapidly and adapt to such large accelerations without disrupting internal architecture. To address this, we investigate Spirostomum ambiguum-a giant cell (1-4 mm in length) well-known to exhibit ultrafast contractions (50% of body length) within 5 ms with a peak acceleration of 15[Formula: see text]. Utilizing transmitted electron microscopy and confocal imaging, we identify an association of rough endoplasmic reticulum (RER) and vacuoles throughout the cell-forming a contiguous fenestrated membrane architecture that topologically entangles these two organelles. A nearly uniform interorganelle spacing of 60 nm is observed between RER and vacuoles, closely packing the entire cell. Inspired by the entangled organelle structure, we study the mechanical properties of entangled deformable particles using a vertex-based model, with all simulation parameters matching 10 dimensionless numbers to ensure dynamic similarity. We demonstrate how entangled deformable particles respond to external loads by an increased viscosity against squeezing and help preserve spatial relationships. Because this enhanced damping arises from the entanglement of two networks incurring a strain-induced jamming transition at subcritical volume fractions, which is demonstrated through the spatial correlation of velocity direction, we term this phenomenon "topological damping." Our findings suggest a mechanical role of RER-vacuolar meshwork as a metamaterial capable of damping an ultrafast contraction event.

Tracing past extreme floods on an alluvial fan using geophysical surveying

SummarySedimentary units in alluvial fans may record gradual transport and deposition during multiple floods or sediment‐laden flows or, conversely, during few catastrophic events. While outcrops are a valuable source of direct information to constrain past geomorphic and hydrologic processes, such exposures are scarce, especially along aggrading rivers or those that have been subject to recent catastrophic sedimentation. In this context, near‐surface geophysical techniques can constrain the dimensions, internal architecture, composition, and petrophysical properties of different sedimentary units. We consider the Grimmbach alluvial fan in the cuesta landscape of southwestern Germany, which was heavily impacted by sediment and wood loads during a flash flood in 2016; published radiocarbon dates indicate that at least three floods similar to the one in 2016 may have occurred since the 17th century. To test whether and to which detail near‐surface geophysics might reveal the sedimentary legacy of these floods, we survey the Grimmbach alluvial fan using detailed topographic data and geophysical imaging based on electromagnetic induction, electrical resistivity tomography (ERT), and ground‐penetrating radar. Our geophysical results indicate former channel courses and two coarse bar deposits up to 3 m below the surface, which are comparable with the more extensive bar deposits of the 2016 flood. From the ERT models, we interpret coarse, up to 5 m thick, gravel lag overlying bedrock at a maximum depth of 10 m. Our geophysical results also highlight patches of finer materials derived from gradual sedimentation and soil development. Overall, our results show that the Grimmbach alluvial fan may have formed and reshaped during catastrophic flows, which likely caused channel avulsions. Our findings point to the need to reconsider flash flood and debris‐flow hazards in similar headwaters and fans of this seemingly quiescent cuesta landscape in southern Germany.

Development and optimisation of hydroxyapatite-polyethylene glycol diacrylate hydrogel inks for 3D printing of bone tissue engineered scaffolds

In the event of excessive damage to bone tissue, the self-healing process alone is not sufficient to restore bone integrity. Three-dimensional (3D) printing, as an advanced additive manufacturing technology, can create implantable bone scaffolds with accurate geometry and internal architecture, facilitating bone regeneration. This study aims to develop and optimise hydroxyapatite-polyethylene glycol diacrylate (HA-PEGDA) hydrogel inks for extrusion 3D printing of bone tissue scaffolds. Different concentrations of HA were mixed with PEGDA, and further incorporated with pluronic F127 (PF127) as a sacrificial carrier. PF127 provided good distribution of HA nanoparticle within the scaffolds and improved the rheological requirements of HA-PEGDA inks for extrusion 3D printing without significant reduction in the HA content after its removal. Higher printing pressures and printing rates were needed to generate the same strand diameter when using a higher HA content compared to a lower HA content. Scaffolds with excellent shape fidelity up to 75-layers and high resolution (∼200 µm) with uniform strands were fabricated. Increasing the HA content enhanced the compression strength and decreased the swelling degree and degradation rate of 3D printed HA-PEGDA scaffolds. In addition, the incorporation of HA improved the adhesion and proliferation of human bone mesenchymal stem cells (hBMSCs) onto the scaffolds. 3D printed scaffolds with 2 wt% HA promoted osteogenic differentiation of hBMSCs as confirmed by the expression of alkaline phosphatase activity and calcium deposition. Altogether, the developed HA-PEGDA hydrogel ink has promising potential as a scaffold material for bone tissue regeneration, with excellent shape fidelity and the ability to promote osteogenic differentiation of hBMSCs.

Temperature-responsive PCL-PLLA nanofibrous tissue engineering scaffolds with memorized porous microstructure recovery.

Biomaterial scaffolds in tissue engineering facilitate tissue regeneration and integration with the host. Poor healing outcomes arise from lack of cell and tissue infiltration, and ill-fitting interfaces between matrices or grafts, resulting in fibrous tissue formation, inflammation, and resorption. Existing tissue engineering scaffolds struggle to recover from deformation to fit irregularly shaped defects encountered in clinical settings without compromising their mechanical properties and favorable internal architecture. This study introduces a synthetic biomaterial scaffold composed of high molecular weight poly (L-lactic acid) (PLLA) and an interpenetrating network of poly (ε-caprolactone) (PCL), in a composition aiming to address the need for conformal fitting synthetic matrices which retain and recover their advantageous morphologies. The scaffold, known as thermosensitive memorized microstructure (TS-MMS), forms nanofibrous materials with memorized microstructures capable of recovery after deformation, including macropores and nanofibers. TS-MMS nanofibers, with 50-500 nm diameters, are formed via thermally induced phase separation (TIPS) of PLLA after in situ polymerization of PCL-diacrylate. A critical partial-melting temperature of TS-MMS at 52°C enables bulk deformation above this temperature, while retaining the nanofibrous and macroporous structures upon cooling to 37°C. Incorporation of drug-loaded poly (lactide-co-glycolide) (PLGA) nanoparticles directly into TS-MMS nanofibers during fabrication allows sustained release of a model drug for up to 40 days. Subcutaneous implantation in vivo using LysM-Cre;td-Tomato; Col1eGFP mice demonstrates successful cellularization and integration of deformed/recovered TS-MMS materials, surpassing the limitations of deformed PLLA scaffolds, to facilitate cell and vasculature infiltration requisite for successful bone regeneration. Additionally we demonstrated a method for embedding controlled release vehicles directly into the scaffold nanofibers; controlled release of simvastatin enhances vascularization and tissue maturation. TS-MMS scaffolds offer promising improvements in clinical handling and performance compared to existing biomaterial scaffolds.

Reconstruction of Holocene relative sea-level from beach ridges of the central west coast of India using GPR and OSL dating

The Holocene sea-level and associated shoreline regression of beach ridges in the central west coast of India have been reconstructed based on internal sedimentary architecture and luminescence chronology. For this purpose, various techniques namely ground penetrating radar (GPR), optically-stimulated luminescence (OSL) dating, and interferometry synthetic aperture radar (InSAR) have been used. The berm and beach-dune boundary of paleo beach ridges have been mapped from the high-resolution sub-surface images and converted into sea-level indicators using modern analogs and OSL ages. GPR reflection data shows a number of seaward dipping radar facies with a few landward dipping facies in paleo beach ridges. In the case of the swale, radar signal has been highly attenuated due to the presence of water and mud causing the appearance of multiple horizontal and parallel positive and negative couplets in the sedimentary layer. The results of the research reveal that the oldest beach ridge was formed around 7.7 kyr BP and sea-level was +2 m above mean sea-level. The average progradation rate of these ridges is 0.14 m/yr. From the grain size analysis, it has been found that the larger share of grain size falls into the classes of fine and moderate sand. The present displacement map of the region, which has been generated from the Sentinel-1 datasets, demonstrates that the average upliftment is 2.5 mm/yr whereas subsidence is 1 mm/yr. The fall of the Holocene relative sea-level with respect to the study region is the consequence of both global and regional events.

Architecture of vibrissae in eight rodent species of Ctenohystrica (Rodentia): A comparative SEM study

Our study was conducted within the framework of comparative functional morphology of mammalian skin (Sokolov 1982) and “Trichomorphology” (Hausmann 1930; 1944; Teerink 1991) focusing in particular on specialized tactile organs such as whiskers (vibrissae). The architecture of vibrissae was studied for the first time using SEM in the relict rodent Laonastes aenigmamus and in some other representatives of the clade Ctenohystrica (Rodentia): Ctenodactylus gundi, Pectinator spekei, Proechimys guyannensis, Echimys sp., Trichomys apereoides, Chinchilla lanigera, and Octodon degus with the aim to identify the specific characters for each species. The vibrissal architecture is specific and polymorphic: the rod shape and structure of the medulla show differences between species of different genera and differ from those of guard hairs and spines of the same species. In L. aenigmamus, the vibrissal medulla is peculiar in its internal structure, which, together with the structure of guard hairs in this species, confirms its phylogenetically distinct position within Ctenohystrica. For the compared species, the complex medulla is with variously arranged systems of keratinized septa and different degrees of development of air spaces between them (ranging from unstructured and irregular to uni- or biserial ladder-like, cellated or pyramidal). The vibrissae have a non-specialized cuticle serving mostly for protection during tactile contacts, which distinguishes it from the diverse and multifunctional cuticle of guard hairs, spines, and quills. The tactile function of vibrissae is corroborated by the presence of scuff marks, scratches and even cuticle loss at the tip and on the ventral side of the rod, a thickened dense cortex and a poorly developed medullar layer but diverse its internal architecture, which provides only a very limited thermal protection, unlike that of the hair pelage. The SEM study of vibrissal architecture is a promising line of research, because a better knowledge of vibrissae may contribute to the biology and biomimetics.

Assessing drivers of high exhumation magnitudes and young cooling ages in the eastern central Andes, southern Peru (13–18°S)

The potential structural controls on exhumation across the southern Peruvian Andes are not well understood, in part due to limited structural studies that co-locate with thermochronometric datasets. We integrate these two datasets and evaluate the relative contribution that fault geometry, magnitude, and shortening rate have on predicted cooling ages. Here we present a balanced cross-section constructed using new structural observations. This section, combined with existing thermochronometer data and a thermokinematic model, investigates the drivers of high exhumation and young canyon thermochronometric ages along the deeply incised Marcapata canyon in southern Peru. Together, these approaches constrain the timing and magnitude of exhumation in this portion of the southern Peruvian Andes and provide a mechanism for documenting how the internal architecture changes along strike.The balanced cross-section (oriented N30E) covers the Subandean Zone to the northeast, the Marcapata canyon on the eastern flank of the southern Peruvian Andes, and the Altiplano-Eastern Cordillera boundary to the southwest (13–18° S). Exhumation is constrained by four low-temperature thermochronometer systems, including apatite and zircon (U-Th)/He (AHe and ZHe, respectively) and fission-track (AFT and ZFT, respectively). The youngest AHe (∼1–3 Ma), AFT (∼3–7 Ma), ZHe (∼4–7 Ma), and ZFT (∼14–17 Ma) ages are located in the center and valley bottom of the Marcapata canyon. The thermokinematically modeled cross-section produces cooling ages determined by fault geometry and kinematics. Reset ZFT ages require burial of Ordovician rocks in excess of 5.5 km above the original 6.5 km depositional depth. We find that the ZFT and ZHe ages in the Eastern Cordillera are sensitive to the history and magnitude of burial, age and location of uplift, and canyon incision. Canyon incision is required to reproduce the youngest canyon thermochronometric ages while slow shortening rates from ∼10 Ma to Present are required to reproduce interfluve thermochronometric ages. Shortening is accommodated by basement faults that feed slip up through three different décollement levels before reaching the surface. The proposed stacked basement geometry sets the first-order cooling signal seen in modeled ages. We determined that the total shortening in this section from the Subandean Zone to the Altiplano is 147.5 km, similar to shortening estimates in an adjacent thermo-kinematically modeled section in the San Gabán canyon 50 km to the southeast. Both the ZHe and ZFT ages in the Marcapata section (4–5 and 14 Ma) are noticeably younger than cooling ages from the San Gabán section (16 and 29 Ma). The Marcapata section's higher magnitude of exhumation is due to a repetition of basement thrusts that continues to elevate the Eastern Cordillera while active deformation occurs in the Subandean Zone. The youngest thermochronometric ages in all four systems are co-located with the overlapping basement thrust geometry. This basement geometry, kinematic sequence of deformation, and canyon incision co-conspire to produce the young cooling ages observed in the Eastern Cordillera.