Souring in oil production is a serious concern due to the environment, operational and occupational health risks involved. Various methods, including the addition of biocides and nitrate, have been used to control the activity of microorganisms, sulfate-reducing bacteria (SRB) in particular, which cause most of the souring problems. Conventional methods for treated souring are expensive and have side effects. The simulation of the action of molybdate ions in treating souring using different injection strategies is the object of this study. UTCHEM software (University of Texas), which was originally developed to simulate advanced oil recovery, was used in this work. The simulations were carried out in four different scenarios. The simulated reservoir model used for scenarios 1, 2, and 3 was built with characteristics similar to the Central Block of the Buracica Oil Field located in the Recôncavo Basin (Brazil). For the simulations for scenario 4, the 5-spot reservoir model was used with different mesh sizes. It was found that the origin and composition of the injection water influences the development and activity of the sulfate-reducing bacteria and that the reinjection of produced water intensifies acidification after an average residence time of the liquid phase in the reservoir. It was also found that the addition of molybdate at the beginning of secondary recovery is an effective way to prevent acidification of oil reservoirs. The intermittent molybdate injection strategies can have similar efficiency in preventing acidification of the reservoirs as the continuous molybdate injection strategy. Efficiency in preventing reservoir acidification is closely related to the distance between the injection and production wells.