Intermittent Disruptions Research Articles

Relationship between expression of laminin and pathological features in human colorectal carcinoma.

AIM:To study the expression and significance of laminin in human colorectal carcinoma.METHODS:Using the monoclonal antibody to laminin and streptavidin-peroxidase immunohistochemical method, the expression of laminin in 63 cases of human colorectal carcinoma tissues was determined.RESULTS:In normal marge intestinal mucosa adjacent to carcinoma, laminin was largely restricted to basement membrane in continuous linear pattern. In contrast, human colorectal carcinomas exhibited a progressive loss of an intact basement membrane that was correlated with decreasing differentiation degree.Well and moderately differentiated tumors exhibited a thin basement membrane with intermittent disruptions, and poorly differentiated tumors exhibited no areas of intact basement membrane. An association was found between lack of basement membrane laminin immunohistochemical staining in colorectal carcinoma and poorly differentiated tumor (P < 0.01).CONCLUSION:Immunohistochemical staining for laminin could provide a very useful indexfor the determination of the differentiation degree of colorectal carcinoma.

Creatine kinase (CK) isoenzyme activities in cardiac surgery

Creatine kinase (CK) isoenzymes were determined on 15 patients undergoing open heart surgery with bypass. The study was performed to examine the relationship between the hypothermia under which the surgery is conducted and the appearance of CK isoenzymes in the serum both during and after surgery. All patients showed dramatic rises in total CK activity commencing during surgery. Myocardial CK (CK-MB or CK-2) was seen in fourteen patients and brain CK (CK-BB or CK-1) was seen in ten patients. Peak activities of CK-BB did not coincide with peak activities of CK-MB. The serum elevations of CK-BB in these patients appear to arise from a mechanism different from that responsible for the elevations of CK-MB, and it is assumed that the former is due to intermittent disruptions in the perfusion and/or oxygenation of tissues rich in CK-BB. CK-MB elevations appear to be due directly to the surgical intervention. Hypothermia alone does not in itself appear to be solely responsible for elevations of CK-BB although it can not be completely excluded from playing some role in its production.