Intermediate Length Research Articles

Continuous phenotypic modulation explains male horn allometry in three dung beetle species

Many dung beetle species show male horn polyphenism, the ability of males to develop into distinct phenotypes without intermediate forms as a response to the larval growth environment. While males with long (majors) and rudimentary (minor) horn have been widely reported in literature, little is known about the existence of individuals with intermediate horn length. Here we investigate the occurrence of intermediates in natural populations of three dung beetle species (Onthophagus furcatus, Copris lunaris and C. hispanus). We analysed the body size-horn length relationship using linear, exponential, and sigmoidal models with different error structures. We inferred the number of individuals in the minor, intermediate, and major groups by combining changepoint analysis and simulation from fitted allometric models. The sigmoidal equation was a better descriptor of the body size-horn length relationship than linear or exponential equations in all the three studied species. Our results indicated that the number of intermediates equals or exceeds the number of minor and major males. This work provides evidence that, at least in the studied species, males with intermediate horn length exist in natural populations. For similar cases we therefore suggest that continuous phenotypic modulation rather than discrete polyphenism can explain variation in male horn allometry.

Small-Scale Mechanical Testing

This article reviews recent developments in small-scale mechanical property testing with some emphasis on intermediate (meso) length scales in complex microstructures and coated systems. The introduction summarizes size effects discovered from a century ago up to the recent explosion in micropillar testing that established many length scale effects in yielding and fracture. The bulk of the article deals with plasticity and fracture in polyphasic and microstructurally graded systems, including biomaterials, composites, and thermal protection systems, highlighting the use of in situ methods where mechanical tests are coupled to synchrotron X-ray scattering, electron backscattering, radiation damage, and digital image correlation.

The Clinical and Polynucleotide Repeat Expansion Analysis of ATXN2, NOP56, AR and C9orf72 in Patients With ALS From Mainland China.

BackgroundRepeat expansions, including those in C9orf72 and ATXN2, have been implicated in amyotrophic lateral sclerosis (ALS). However, there have been few studies on the association of AR and NOP56 repeat expansion with ALS, especially in China. Accordingly, we aimed to evaluate the frequency of C9orf72 and ATXN2 repeat mutations and investigate whether NOP56 and AR repeat expansion are risk factors for ALS.MethodsIn this study, 736 ALS patients and several hundred healthy controls were recruited. Polymerase chain reaction (PCR) and repeat-primed PCR (RP-PCR) were performed to determine the repeat lengths in C9orf72, ATXN2, AR, and NOP56.ResultsGGGGCC repeats in C9orf72 were observed in six ALS patients (0.8%, 6/736) but not in any of the controls (0/365). The patients with pathogenic GGGGCC repeats showed shorter median survival times than those with a normal genotype (p = 0.006). Regarding ATXN2 CAG repeats, we identified that intermediate repeat lengths (29–34 copies) were associated with ALS (p = 0.033), and there was no difference in clinical characteristics between the groups with and without intermediate repeats (p > 0.05). Meanwhile, we observed that there was no association between the repeat size in AR and NOP56 and ALS (p > 0.05).ConclusionsOur results demonstrated that pathogenetic repeats in C9orf72 are rare in China, while intermediate CAG repeats in ATXN2 are more frequent but have no effect on disease phenotypes; the repeat size in AR and NOP56 may not be a risk factor for ALS.

Monoclinic symmetry at the nanoscale in lead-free ferroelectric BaZrxTi1−xO3 ceramics

Local structural symmetries play a key role in the functionalities of ferroelectric materials and are often found different from average symmetry. Here, we study the real space nanoscale structure in Pb-free BaZr$_{x}$Ti$_{1-x}$O$_{3}$ (x $\leq$ 0.10) by pair distribution function measurements, complemented by transmission electron microscopy and x-ray diffraction. Our observations show existence of the rhombohedrally distorted unit cells; however, at intermediate length scales, at least up to 5 nm, there exist nano-scale correlated regions of monoclinic symmetry. This is complemented by the observation of curved frustrated nanodomains. Further, the average structure is found to have coexisting monoclinic and rhombohedral symmetries. Our observation of a two-phase ferroelectric state is in contrast to interferroelectric instabilities of conventional polymorphic phase boundaries reported for doped BaTiO$_{3}$.

Oblique Packing and Tunable Excitonic Coupling in DNA‐Templated Squaraine Rotaxane Dimer Aggregates

AbstractWhen molecules are aggregated such that their excited states form delocalized excitons, their spatial arrangement, or packing, can be coarsely controlled by templating and finely controlled by chemical substitution; however, challenges remain in controlling their packing on intermediate length scales. Here, we use an approach based on mechanically interlocked molecules to promote an elusive oblique packing arrangement in a series of three squaraine rotaxane dimers. We template the squaraine rotaxane dimers using DNA and observe two excitonically split bands of near‐equal intensity in their absorption spectra – a distinct signature of oblique packing, validated by theoretical modeling of the experimental results. Additional fine control of packing is demonstrated by fluorinating the macrocycle of the rotaxane, which promotes denser packing and stronger excitonic interactions.

Non-thermal argon plasma jets of various lengths for selective reactive oxygen and nitrogen species production

Plasma jets simultaneously create a wide range of reactive oxygen species (ROS) and reactive nitrogen species (RNS), making selective production challenging. In this study, an attempt was made to address this problem by adjusting the downstream length of the plasma jet to regulate reactive species production in an AC-driven argon plasma jet. Jets with short downstream lengths are dominated by RNS, whereas longer ones are dominated by ROS. The energy impact collision between energetic electrons and diffused ambient air is primarily responsible for the generation of RNS in argon plasma. Short plasma jets have a higher electron temperature in the plasma effluent, allowing for better excitation and ionization of N2 molecules. In contrast, in longer jets, electrons transfer most of their energy to neutrals and moisture, even before the diffusion of ambient air. Thus, the production of OH radicals and H2O2 is dominant in longer plasma jets. Further, the ratio of NO2- to H2O2 in plasma-activated water decreased from 1.8 to 0.07, as the downstream length was increased from 1 cm to 10 cm. RNS-enriched short plasma jets are appropriate for immune cell activation, jets of intermediate length are suitable for gold nanoparticle formation because of the synergistic action of ROS and electron energy, and ROS-dominated longer plasma jets are excellent for methylene blue degradation. These findings are applicable to the design of plasma devices in which the generation of ROS and RNS in practical quantities is required to realize plasma technology for a wide range of applications.

Kinetics of dehydrogenation of n-octane on a promoted Pt-catalyst

In contrast to short as well as long chain paraffin dehydrogenation, the highly desirable reaction of intermediate chain length paraffins (C6 - C9) to the corresponding linear monoolefins receives only little attention in the literature. Consequently, the dehydrogenation of the model substance n-octane on promoted platinum catalysts was studied thoroughly. In the first instance, the effect of the addition of different promotors (Sn, K, Ce) on the catalytic performance with emphasis on activity and octene selectivity was tested by screening of 16 differently promoted Pt-catalysts. The optimized and non-deactivated catalyst was then used to investigate the kinetics of n-octane dehydrogenation including the unwanted consecutive formation of n-octadienes and C8-aromatics. The analysis revealed that the desired n-octenes are rapidly converted to n-octadienes, i.e. the thermodynamic equilibrium of this reversible reaction is quickly established. To a small extent, the n-octadienes are further converted to C8-aromatics. A kinetic model was developed, which also considers the inhibiting effect of n-octenes on the rate of n-octane conversion. The model accurately predicts the formation of mono- and diolefins as well as of aromatics in the initial reaction phase in the absence of deactivation.

Improving constraints on the reionization parameters using 21-cm bispectrum

Radio interferometric experiments aim to constrain the reionization model parameters by measuring the 21-cm signal statistics, primarily the power spectrum. However the Epoch of Reionization (EoR) 21-cm signal is highly non-Gaussian, and this non-Gaussianity encodes important information about this era. The bispectrum is the lowest order statistic able to capture this inherent non-Gaussianity. Here we are the first to demonstrate that bispectra for large and intermediate length scales and for all unique k-triangle shapes provide tighter constraints on the EoR parameters compared to the power spectrum or the bispectra for a limited number of shapes of k-triangles. We use the Bayesian inference technique to constrain EoR parameters. We have also developed an Artificial Neural Network (ANN) based emulator for the EoR 21-cm power spectrum and bispectrum which we use to remarkably speed up our parameter inference pipeline. Here we have considered the sample variance and the system noise uncertainties corresponding to 1000 hrs of SKA-Low observations for estimating errors in the signal statistics. We find that using all unique k-triangle bispectra improves the constraints on parameters by a factor of 2 – 4 (depending on the stage of reionization) over the constraints that are obtained using power spectrum alone.

Intermediate length scale of water jets under gravity: An experimental result

Intermediate length scale of water jets under gravity: An experimental result

Leg Dominance Does Not Influence Maximal Force, Force Steadiness, or Motor Unit Discharge Characteristics.

The aim of our study was to compare maximal force, force steadiness, and discharge characteristics of motor units in tibialis anterior during contractions with the dorsiflexors of the dominant and nondominant legs at low-to-moderate target forces and three ankle angles. Twenty young adults performed maximal and submaximal isometric contractions (5%, 10%, 20%, 40%, and 60% of maximal voluntary contraction (MVC)) with the dorsiflexors of the dominant and nondominant legs at three ankle angles (75°, short length; 90°, intermediate length; 105°, long length). High-density EMG signals from the tibialis anterior muscle of each leg were recorded. Maximal force (average dominant, 182.9 ± 64.5 N; nondominant, 179.0 ± 58.8 N) and the fluctuations in force, quantified as absolute (SD) and normalized amplitudes (coefficient of variation (CoV)), were similar between the two legs across the three ankle angles (average CoV for dominant, 1.5% ± 1.0%; nondominant, 1.7% ± 1.3%). The CoV for force for both legs decreased from 5% to 20% MVC force, and then it plateaued at 40% and 60% MVC force. EMG amplitude, mean discharge rate of motor units, discharge variability (interspike interval), and the variability in neural drive (filtered cumulative spike train) were similar between the two legs across the submaximal contractions. MVC force and force steadiness were similar across ankle angles and target forces between the dominant and nondominant legs. The attributes that underlie the self-reported identification of a dominant leg were not associated with the force capacity or the control of force for the dorsiflexor muscles, at least during isometric contractions.

CD36-Binding Amphiphilic Nanoparticles for Attenuation of Alpha Synuclein-Induced Microglial Activation.

Neuroinflammation is one of the hallmarks contributing to Parkinson's Disease (PD) pathology, where microglial activation occurs as one of the earliest events, triggered by extracellular alpha synuclein (aSYN) binding to the CD36 receptor. Here, CD36-binding nanoparticles (NPs) containing synthetic tartaric acid-based amphiphilic polymers (AMs) were rationally designed to inhibit this aSYN-CD36 binding. In silico docking revealed that four AMs with varying alkyl side chain lengths presented differential levels of CD36 binding affinity and that an optimal alkyl chain length would promote the strongest inhibitory activity towards aSYN-CD36 interactions. In vitro competitive binding assays indicated that the inhibitory activity of AM-based NPs plateaued at intermediate side chain lengths of 12- and 18-carbons, supporting the in silico docking predictions. These 12- and 18-carbon length AM NPs also had significantly stronger effects on reducing aSYN internalization and inhibiting the production of the proinflammatory molecules TNF-α and nitric oxide from aSYN-challenged microglia. All four NPs modulated the gene expression of aSYN-challenged microglia, downregulating the expression of the proinflammatory genes TNF, IL-6, and IL-1β, and upregulating the expression of the anti-inflammatory genes TGF-β and Arg1. Overall, this work represents a novel polymeric nanotechnology platform that can be used to modulate aSYN-induced microglial activation in PD.

Nighttime Sleep Quality and Daytime Sleepiness Predicts Suicide Risk in Adults Admitted to an Inpatient Psychiatric Hospital

ABSTRACT As sleep problems have been identified as an important, yet understudied, predictor of suicide risk, the present study analyzed the relationship between daytime sleepiness and nighttime sleep disturbance in a high-risk population of adults admitted to an inpatient psychiatric hospital. Objectives were to (1) examine the time course of subjective daytime sleepiness, nighttime sleep disturbance, and suicide risk throughout inpatient psychiatric treatment, (2) examine pre- to post-treatment changes in sleep disturbance with treatment as usual in an inpatient psychiatric setting, and (3) investigate whether daytime sleepiness and nighttime sleep disturbance predicted suicide risk above and beyond anxiety and depression. Participants were 500 consecutively admitted adults admitted to an intermediate length of stay (4–6 weeks) inpatient psychiatric hospital (47% female; 18–87 years of age). Measures of sleep, suicide risk, depression, and anxiety were completed at admission, weeks 1 through 4, and at discharge. Latent growth curve modeling (LGM) and hierarchal linear modeling (HLM) were conducted. The LGM analysis demonstrated that daytime sleepiness, nighttime sleep disturbance, and suicide risk all improved throughout inpatient treatment. Further, HLM showed that daytime sleepiness predicted suicide risk above and beyond symptoms of anxiety, depression, major sleep medications, and prior suicidal ideation and attempts, while nighttime sleep disturbance predicted suicide risk above and beyond symptoms of anxiety, major sleep medications, and prior suicidal ideation and attempts. Findings indicate the need to reevaluate safety protocols that may impact sleep, particularly that may increase daytime sleepiness, and to develop evidence-based sleep interventions for individuals admitted to inpatient psychiatric hospitals.

Comparison of Short, Intermediate, and Long Cephalomedullary Nail Length Outcomes in Elderly Intertrochanteric Femur Fractures.

Introduction:Cephalomedullary nail (CMN) length for intertrochanteric femur fractures without subtrochanteric extension has been an ongoing debate. The authors hypothesize that increasing nail length would result in increasing surgical time, greater incidence of acute kidney injury (AKI), postoperative anemia, and blood loss requiring transfusion due to increased intramedullary reaming and pressurization of the canal with nail insertion.Methods:A retrospective chart review of patients aged 65 years or older who underwent CMN for low-energy intertrochanteric femur fractures from 2010 to 2018 was undertaken. Patient demographic data, comorbidities, case duration, postoperative hospital length of stay (LOS), and laboratory data, including serum creatinine, hemoglobin, and hematocrit, were collected for analysis. The following outcome measures were compared: postoperative pneumonia, cardiac complications, sepsis, reintubation/intensive care unit stay, pulmonary embolism, stroke, postoperative AKI, 30-day hospital readmission, 30-day return to operating room, 30-day mortality, 1-year mortality, postoperative anemia (hemoglobin <7 g/dL), and blood transfusion.Results:A total of 247 patients were analyzed (short = 48, intermediate = 39, and long = 160). No notable difference was observed in postoperative pneumonia, cardiac complications, sepsis, reintubation/intensive care unit stay, pulmonary embolism, stroke, mean total hospital LOS, mean postoperative hospital LOS, rate of postoperative AKI, 30-day readmission, 30-day return to operating room, 30-day mortality, or 1-year mortality. Patients receiving long nails had significantly higher rates of postoperative anemia (P = 0.0491), blood transfusion (P = 0.0126), and mean procedure length (P = 0.0044) compared with the two other groups.Discussion:Patients receiving long nails had markedly higher rates of postoperative anemia and blood loss requiring blood transfusion with markedly longer mean procedure length than patients receiving short and intermediate CMNs. Long nails did not result in an increase in other complications evaluated.

Selective stabilization of the intermediate inactivated Na+ channel by the new-generation anticonvulsant rufinamide

Na+ channels undergo multiple inactivated states with different kinetics, which set the refractory period of neuronal discharges, but isolating the intermediate inactivated state has been challenging. Most classical Na+channel-inhibiting anticonvulsants bind to the fast inactivated state to reduce Na+currents and cellular excitability. These anticonvulsants have the slow binding kinetics and thus necessitate long depolarization for drug action, a “use-dependent” effect sparing most normal activities. Rufinamide is a new anticonvulsant targeting Na+channels, and has a therapeutic effect on Lennox-Gastaut syndrome (LGS) which is refractory to classicalNa+channel inhibitors. The efficacy on LGS, whose epileptiform discharges largely involve short depolarization or bursts, is primarily due to the very fast binding kinetics of rufinamide. Could the very fast kinetics of rufinamide lead to indiscriminate inhibition of neuronal activities ? Onhippocampal neurons from male and female mice, wefound that rufinamide most effectively shifts the Na+channel inactivation curve if the inactivating pulse is 1 s, rather than 0.1 or 18 s, in duration. Rufinamide also shows a maximal slowing effect on the recovery kinetics from the inactivation driven by modest depolarization (e.g. −60 mV) of intermediate length (e.g. 50–300 ms). Consistently, rufinamide selectively inhibits the burst discharges at 50–300 ms on a plateau of ∼−60 mV. This is mechanistically ascribable to selective binding of rufinamide to an intermediate inactivated state withan apparent dissociation constantof ∼40 μM. Being the first molecule embodying the evasive transitional gating state, rufinamide could have a unique anti-seizure profile with a novel form of use-dependent action.

Dark sound: Collective modes of the axionic dark matter condensate

We discuss the axion dark matter (DM) condensate and the consequences the interactions of dark matter would have on the spectrum of collective modes. We find that DM self-interactions change the spectrum of excitations from a quadratic to a linearlike dispersion with velocity ${v}_{s}$ which is set by the interactions, but dominated by gravity. For typical DM densities and interactions we find ${v}_{s}\ensuremath{\sim}{10}^{\ensuremath{-}12}c$. This soundlike mode corresponds to DM density oscillations just like in any other Bose liquid, hence we call it dark sound (DS). The DS mode is well defined and describes stable density oscillations at intermediate length scales $k\ensuremath{\ge}{k}_{\mathrm{min}}\ensuremath{\sim}{10}^{4}\text{ }\text{ }{\mathrm{lyr}}^{\ensuremath{-}1}$. In the extreme long-wavelength limit gravity dominates and leads to Jeans instability of the sound mode at the scale of clump formation $k\ensuremath{\le}{k}_{\mathrm{min}}$. We also discuss the possible observable consequences of the DS, including quantized DS modes inside clumps, their characteristic energy, and noise features that might facilitate the observation of DM.

Hypertension in pregnancy and adverse outcomes among low-risk nulliparous women expectantly managed at or after 39weeks: a secondary analysis of a randomised controlled trial.

To evaluate whether hypertensive disorders of pregnancy (HDP) among low-risk nulliparous women expectantly managed at or after 39weeks of gestation are associated with adverse outcomes. Secondary analysis of a randomised trial. Multicentre, USA. Individuals in the expectantly managed group who delivered on or after 39weeks. Multivariable analysis to estimate adjusted relative risks (aRR) for binomial outcomes, adjusted odds ratios (aOR) for multinomial outcomes and 95% CI. Composite adverse maternal outcome including placental abruption, pulmonary oedema, postpartum haemorrhage, postpartum infection, venous thromboembolism or intensive care unit admission. Secondary outcomes included a composite of perinatal death or severe neonatal complications, mode of delivery, small and large for gestational age and neonatal intermediate or intensive unit length of stay. Of the 3044 women randomised to expectant management in the original trial, 2718 (89.3%) were eligible for this analysis, of whom 373 (13.7%) developed HDP. Compared with participants who remained normotensive, those who developed HDP were more likely to experience the maternal composite (12% versus 6%, aRR 1.84, 95% CI 1.33-2.54) and caesarean delivery (29% versus 23%, aOR 1.32, 95% CI 1.01-1.71). Differences between the two groups were not significantly different for the adverse perinatal composite (7% versus 5%, aRR 1.38, 95% CI 0.92-2.07) or for other secondary outcomes. Almost 14% of low-risk nulliparous individuals expectantly managed at 39weeks developed HDP, and were more likely to experience adverse maternal outcomes compared with those who did not develop HDP. Almost 14% of low-risk nulliparous individuals expectantly managed at 39 weeks developed hypertensive disorders of pregnancy, and were more likely to experience adverse maternal outcomes compared with those who did not develop hypertensive disorders.

Functional Outcomes Following Operative Management of Ankle Fractures: Intermediate Length Follow-Up

Category:Ankle; Ankle Arthritis; TraumaIntroduction/Purpose:Ankle fractures are among the most commonly surgically treated orthopaedic injuries, with debate regarding their long-term outcomes and the clinical benefit of contemporary fixation strategies. The purpose of this study was to retrospectively evaluate the functional outcomes of patients following surgical management of ankle fractures, and to determine what, if any, limitations and effects their injury has on their daily lives. The authors of this study hypothesized that despite patients having some degree of measurable impairments, these did not cause meaningful disruption in their everyday lives.Methods:We retrospectively reviewed a total of 259 patients who underwent surgical management of their ankle fractures between 2004 and 2012. Two separate patient reported outcome measures, the Foot Function Index (FFI) and Short Musculoskeletal Function Assessment (SMFA) were obtained at a minimum of 5 years following time of surgery. Multivariate linear regression analysis was performed to compare FFI and SMFA against several patient and injury specific variables.Results:A total of 259 patients with ankle fractures were included in the study population. 7 of the demographic and injury- related variables were found to have a statistically significant relationship to both the FFI and SMFA scores. The multivariate linear regression analysis using these 7 variables in addition to both sub-group components of the SMFA to predict the total FFI score yielded an output adjusted-r2 = 0.78. The SMFA dysfunction sub-category was found to correlate with FFI (standardized beta 0.85, p<0.001), but the SMFA bothersome sub-category did not (p=0.866). Ankle pain limiting function and Medicaid insurance status were also predictive of higher FFI scores.Conclusion:Despite patients, at times, experiencing persistent issues with pain and mild activity limitations following surgical management of ankle fractures, these did not lead to measured burdens in daily activities. At a moderate-length follow-up, patients overall do well from a functional standpoint after sustaining ankle fractures treated surgically.

A model on an absolute scale for the small-angle X-ray scattering from bovine casein micelles.

Casein micelles extracted from milk are 100-400 nm-sized particles, made up of proteins and calcium phosphates, with the latter as colloidal calcium phosphate particles (CCPs) in a size range of 2-4 nm embedded in a protein network. The hierarchical structures give rise to a variation of scattering intensity over many orders of magnitude, which can be measured by small-angle X-ray scattering and static light scattering. Expressions for the scattering intensity of a general simple model for composite particles with polydispersities of overall size and subparticles are derived, and some approximations are checked by generating scattering data for systems generated by Monte Carlo simulations. Based on the simpler models, a new model has been developed for casein micelles, where the scattering is expressed on an absolute scale and where the concentrations of, respectively, protein and CCPs are used as constraints, providing a consistent model. The CCPs are modelled as oblate ellipsoids and the protein as star structures. Correlations between the substructures of CCPs and protein structures are taken into account in terms of partial structure factors. The overall structure as well as some heterogeneities at intermediate length scale are modelled as polydisperse spheres. The model fits the data very well on all length scales and demonstrates that both the scattering from CCPs and protein is important. Thus, the model provides a detailed description of the casein structure, which is consistent with the information available in the literature.

Connecting Packing Efficiency of Binary Hard Sphere Systems to Their Intermediate Range Structure.

Using computed x-ray tomography we determine the three dimensional (3D) structure of binary hard sphere mixtures as a function of composition and size ratio of the particles q. Using a recently introduced four-point correlation function we reveal that this 3D structure has on intermediate and large length scales a surprisingly regular order, the symmetry of which depends on q. The related structural correlation length has a minimum at the composition at which the packing fraction is highest. At this composition also the number of different local particle arrangements has a maximum, indicating that efficient packing of particles is associated with a structure that is locally maximally disordered.

Aroyloxycinnamates with wide mesophase temperature ranges

ABSTRACT A new series of eight asymmetric homologues was designed and synthesised by the reaction of aroyloxybenzaldehydes with Wittig salt of methyl bromoacetate, where the length of the alkoxy chain was varied. All the compounds were found thermally stable up to 220°C. Differential scanning calorimetry, polarising optical microscopy and X-ray diffraction were used to study the mesomorphic properties, where a mesophase range of up to 80°C was obtained. The two shorter homologues with C3–C4 were nematic, the intermediate lengths were nematic and smectic A (SmA) with a progressive increase of the SmA domains and the longest homologue with C12 was purely SmA. The translational order parameter of the SmA mesophases showed that the molecular order inside the smectic layers increases with molecular length, associated with the higher stabilisation and predominance of the SmA phase. All compounds dispersed in solution absorb light in the ultraviolet region.