Intermediate Divisions Research Articles

Dopamine transmission in the tail striatum: Regional variation and contribution of dopamine clearance mechanisms.

The striatum can be divided into four anatomically and functionally distinct domains: the dorsolateral, dorsomedial, ventral and the more recently identified caudolateral (tail) striatum. Dopamine transmission in these striatal domains underlies many important behaviours, yet little is known about this phenomenon in the tail striatum. Furthermore, the tail is divided anatomically into four divisions (dorsal, medial, intermediate and lateral) based on the profile of D1 and D2 dopamine receptor-expressing medium spiny neurons, something that is not seen elsewhere in the striatum. Considering this organisation, how dopamine transmission occurs in the tail striatum is of great interest. We recorded evoked dopamine release in the four tail divisions, with comparison to the dorsolateral striatum, using fast-scan cyclic voltammetry in rat brain slices. Contributions of clearance mechanisms were investigated using dopamine transporter knockout (DAT-KO) rats, pharmacological transporter inhibitors and dextran. Evoked dopamine release in all tail divisions was smaller in amplitude than in the dorsolateral striatum and, importantly, regional variation was observed: dorsolateral ≈ lateral > medial > dorsal ≈ intermediate. Release amplitudes in the lateral division were 300% of that in the intermediate division, which also exhibited uniquely slow peak dopamine clearance velocity. Dopamine clearance in the intermediate division was most dependent on DAT, and no alternative dopamine transporters investigated (organic cation transporter-3, norepinephrine transporter and serotonin transporter) contributed significantly to dopamine clearance in any tail division. Our findings confirm that the tail striatum is not only a distinct dopamine domain but also that each tail division has unique dopamine transmission characteristics. This supports that the divisions are not only anatomically but also functionally distinct. How this segregation relates to the overall function of the tail striatum, particularly the processing of multisensory information, is yet to be determined.

Comparison of monkey and human retrosplenial neurocytology.

Retrosplenial cortex (RSC) has unique problems for human neuroimaging studies as its divisions are small, at the lower end of functional scanner spatial resolution, and it is buried in the callosal sulcus. The present study sought to define the cytoarchitecture of RSC in human and monkey brains along its entire anteroposterior extent. The results show anterior extensions, a newly defined dichotomy of area 30, a new area p30, and an area p29v in monkey that differentiates into three divisions in human. Accordingly, anterior (a), intermediate (i), and posterior (p) divisions of areas 29l, 29m, 30l, and 30m were identified. Posterior area 29 has higher neuron packing in the granular layer than anterior and intermediate divisions of area 29. A newly detected dysgranular area p30 has larger neurons in layers II-IIIab than a30 and i30 and with substantially higher NFP expression in layer IIIab of posterior areas than areas a30 and i30. Medial area 30 has larger pyramids and higher NFP expression in all layers than area 30l. The new area p30 was seen between areas p29m and p30I in both species. Finally, a ventral area p29v is present in monkeys. This latter area appears to differentiate into three divisions in human with the most extensive granular layer adjacent to layer I in p29vm and p29vl. Functional imaging has identified pRSC as part of a cognitive map which is engaged in spatial navigation and localization of personally relevant objects.

System of redistribution of non-used resource work in non-rhythmic flow-lines

The article proposes to reduce the duration of non-rhythmic flow lines on technologically identical processes by using the resource of «flow-line non-critical» divisions on interdependent works. In non-rhythmic flow-line construction, there are processes with a reserve of non-used resources with adjacent works («flow-line non-rhythmic» works). As a result of the system study, it is proposed to characterize «flow-line non-critical» works as having an increased period of deployment or convolution of flow lines. The work volume is redistributed on technologically identical processes, for which interdependent divisions are determined. General new working deadlines are limited by the target function. Additional restrictions determine the limits of change in the term of work on intermediate divisions. Optimized works are performed in two variants for three and two shifts. This method is more effective in reducing construction and complements the optimization method by reducing the deployment period.

Intermediate Parts of Motion According to Ramon Llull: Some Remarks About His Medieval Background

Following Aristotle, Averroes rejects atomism and the infinite division of geometric lines. Thus, his arguments dealt with the continuity and contiguity of the non-atomic parts of motion. He vindicates the perceptual aspect of physical movement that shows itself like in-progress-path between two edge points A and B, in which there are middle parts where qualitative, local, or quantitative changes occur. Ramon Llull took the lines’ geometrical points as “motion parts.” Points are intermediate divisions that represent physical phenomena by the continuity of geometrical lines, surfaces, and figures. Also, he appeals to relational logic to spot the middle parts between A and B into the in-progress-path of motion. Those middle parts are signified by a dynamic vocabulary, called: correlative language. This contribution focuses on the conceptual environment of Llull’s assumptions, in which Averroes’ Latin readers explored the geometry and the vocabulary of motion intermediate parts.

The Tail of the Mouse Striatum Contains a Novel Large Type of GABAergic Neuron Incorporated in a Unique Disinhibitory Pathway That Relays Auditory Signals to Subcortical Nuclei.

The most caudal part of the striatum in rodents, the tail of the striatum (TS), has many features that distinguish it from the rostral striatum, such as its biased distributions of dopamine receptor subtypes, lack of striosomes and matrix compartmentalization, and involvement in sound-driven behaviors. However, information regarding the TS is still limited. We demonstrate in this article that the TS of the male mouse contains GABAergic neurons of a novel type that were detected immunohistochemically with the neurofilament marker SMI-32. Their somata were larger than cholinergic giant aspiny neurons, were located in a narrow space adjacent to the globus pallidus (GP), and extended long dendrites laterally toward the intermediate division (ID) of the trilaminar part of the TS, the region targeted by axons from the primary auditory cortex (A1). Although vesicular glutamate transporter 1-positive cortical axon terminals rarely contacted these TS large (TSL) neurons, glutamic acid decarboxylase-immunoreactive and enkephalin-immunoreactive boutons densely covered somata and dendrites of TSL neurons, forming symmetrical synapses. Analyses of GAD67-CrePR knock-in mice revealed that these axonal boutons originated from nearby medium spiny neurons (MSNs) in the ID. All MSNs examined in the ID in turn received inputs from the A1. Retrograde tracers injected into the rostral zona incerta and ventral medial nucleus of the thalamus labeled somata of TSL neurons. TSL neurons share many morphological features with GP neurons, but their strategically located dendrites receive inputs from closely located MSNs in the ID, suggesting faster responses than distant GP neurons to facilitate auditory-evoked, prompt disinhibition in their targets.SIGNIFICANCE STATEMENT This study describes a newly found population of neurons in the mouse striatum, the brain region responsible for appropriate behaviors. They are large GABAergic neurons located in the most caudal part of the striatum [tail of the striatum (TS)]. These TS large (TSL) neurons extended dendrites toward a particular region of the TS where axons from the primary auditory cortex (A1) terminated. These dendrites received direct synaptic inputs heavily from nearby GABAergic neurons of the striatum that in turn received inputs from the A1. TSL neurons sent axons to two subcortical regions outside basal ganglia, one of which is related to arousal. Specialized connectivity of TSL neurons suggests prompt disinhibitory actions on their targets to facilitate sound-evoked characteristic behaviors.

Neuronal and glial characterization in the rostrocaudal axis of the human anterior olfactory nucleus: Involvement in Parkinson's disease.

Hyposmia is one of the prodromal symptoms of Parkinson’s disease (PD) and a red flag in clinical diagnosis. Neuropathologically, this sign correlates with α-synuclein involvement in the anterior olfactory nucleus (AON). Neurodegeneration, microgliosis, and astrogliosis in AON are poorly studied, and bulbar AON is the focus of these studies with contradictory results. Additionally, male sex is a risk marker for developing PD, but sexual dimorphism of neural and glial populations in the AON has rarely been considered. The aim of this study was to analyze the density of NeuN, Iba-1, GFAP, and Lewy bodies (LBs), as well as the relationship of these cell type markers with pathology along the rostrocaudal axis of the AON (bulbar, retrobulbar, cortical anterior, and posterior divisions). Cavalieri, optical fractionator, and area fraction fractionator stereological approaches were used for the volume, cell populations and LBs densities, area fraction, and percentage of overlap. Iba-1 and α-syn intensities were measured using ImageJ. In non-PD (NPD) cases, the volume was lower in the AON at the extremes of the rostrocaudal axis than in the intermediate divisions. Cortical anterior AON volume decreased in PD compared with NPD cases. NeuN density decreased rostrocaudally in AON portions in NPD and PD cases. This occurred similarly in Iba-1 but only in PD samples. Iba-1 intensity significantly increased in bulbar AON between PD and NPD. No changes were found in astrocytes. Eight percent of NeuN, 0.1% of Iba-1, and 0.1% of GFAP areas overlapped with LBs area along the AON portions. The data indicate that bulbar AON, which is the most rostral portion in this axis, could play a major role in the pathology. This could be related to the larger area occupied by LBs in these divisions.

Topography of the common fibular nerve terminal division in human foetuses.

The progress of paediatric surgery and increasingly better diagnosis of foetal defects require detailed knowledge of human developmental anatomy. Precise knowledge of the anatomy of innervation of the lower extremities corresponds to this subject and is not only cognitive but also clinically important. The end of the common fibular nerve is superficially located in the area exposed to frequent injuries as well as in the area subject to possible surgical repair procedures. The analysis was carried out on 200 human foetuses aged from the 113th day to 222nd day of foetal life. The study material is a part of local foetal collection. The study incorporated the following methods: anthropological, preparational and image acquisition which was acquired with the use of high-resolution digital camera. Statistical analysis was carried out with the use of STATISTICA package. Based on the research results the new typology of the examined nerve was determined. The head of the fibula was the criterion: (i) high division - above the head of the fibula (1%); (ii) intermediate division - at the height of the head of the fibula (34%); (iii) low division - below the head of the fibula (65%). The mathematical analysis did not reveal statistically significant bilateral and gender differences. Moreover the additional branch was observed in 30% of foetuses, regardless of age class. This branch occurred in 50% of cases in both sides of the foetus. This nerve was defined as the accessory fibular nerve (nervous fibularis/peroneus accessorius). The created unique typology of the terminal division of common fibular nerve is an important supplement to the anatomical knowledge and at the same time, due to the peripheral and superficial location of the described structures, it has a relatively high clinical significance.

Microstructural properties within the amygdala and affiliated white matter tracts across adolescence

The amygdala is a heterogenous set of nuclei with widespread cortical connections that continues to develop postnatally with vital implications for emotional regulation. Using high-resolution anatomical and multi-shell diffusion MRI in conjunction with novel amygdala segmentation, cutting-edge tractography, and Neurite Orientation Dispersion and Density (NODDI) methods, the goal of the current study was to characterize age associations with microstructural properties of amygdala subnuclei and amygdala-related white matter connections across adolescence (N = 61, 26 males; ages of 8–22 years). We found age-related increases in the Neurite Density Index (NDI) in the lateral nucleus (LA), dorsal and intermediate divisions of the basolateral nucleus (BLDI), and ventral division of the basolateral nucleus and paralaminar nucleus (BLVPL). Additionally, there were age-related increases in the NDI of the anterior commissure, ventral amygdalofugal pathway, cingulum, and uncinate fasciculus, with the strongest age associations in the frontal and temporal regions of these white matter tracts. This is the first study to utilize NODDI to show neurite density of basolateral amygdala subnuclei to relate to age across adolescence. Moreover, age-related differences were also notable in white matter microstructural properties along the anterior commissure and ventral amydalofugal tracts, suggesting increased bilateral amygdalae to diencephalon structural connectivity. As these basolateral regions and the ventral amygdalofugal pathways have been involved in associative emotional conditioning, future research is needed to determine if age-related and/or individual differences in the development of these microstructural properties link to socio-emotional functioning and/or risk for psychopathology.

Three divisions of the mouse caudal striatum differ in the proportions of dopamine D1 and D2 receptor-expressing cells, distribution of dopaminergic axons, and composition of cholinergic and GABAergic interneurons

The greater part of the striatum is composed of striosomes and matrix compartments, but we recently demonstrated the presence of a region that has a distinct structural organization in the ventral half of the mouse caudal striatum (Miyamoto et al. in Brain Struct Funct 223:4275–4291, 2018). This region, termed the tri-laminar part based upon its differential immunoreactivities for substance P and enkephalin, consists of medial, intermediate, and lateral divisions. In this study, we quantitatively analyzed the distributions of both projection neurons and interneurons in each division using immunohistochemistry. Two types of projection neurons expressing either the dopamine D1 receptor (D1R) or D2 receptor (D2R) showed complementary distributions throughout the tri-laminar part, but the proportions significantly differed among the three divisions. The proportion of D1R-expressing neurons in the medial, intermediate, and lateral divisions was 88.6 ± 8.2% (651 cells from 3 mice), 14.7 ± 3.8% (1025 cells), and 49.3 ± 4.5% (873 cells), respectively. The intermediate division was further characterized by poor innervation of tyrosine hydroxylase immunoreactive axons. The numerical density of choline acetyltransferase immunoreactive neurons differed among the three divisions following the order from the medial to lateral divisions. In contrast, PV-positive somata were distributed throughout all three divisions at a constant density. Two types of GABAergic interneurons labeled for nitric oxide synthase and calretinin showed the highest cell density in the medial division. The present results characterize the three divisions of the mouse caudal striatum as distinct structures, which will facilitate studies of novel functional loops in the basal ganglia.

Translating Fear Circuitry: Amygdala Projections to Subgenual and Perigenual Anterior Cingulate in the Macaque.

Rodent fear-learning models posit that amygdala–infralimbic connections facilitate extinction while amygdala–prelimbic prefrontal connections mediate fear expression. Analogous amygdala–prefrontal circuitry between rodents and primates is not established. Using paired small volumes of neural tracers injected into the perigenual anterior cingulate cortex (pgACC; areas 24b and 32; a potential homologue to rodent prelimbic cortex) and subgenual anterior cingulate cortex (sgACC, areas 25 and 14c; a potential homologue to rodent infralimbic cortex) in a single hemisphere, we mapped amygdala projections to the pgACC and sgACC within single subjects. All injections resulted in dense retrograde labeling specifically within the intermediate division of the basal nucleus (Bi) and the magnocellular division of the accessory basal nucleus (ABmc). Areal analysis revealed a bias for connectivity with the sgACC, with the ABmc showing a greater bias than the Bi. Double fluorescence analysis revealed that sgACC and pgACC projections were intermingled within the Bi and ABmc, where a proportion were double labeled. We conclude that amygdala inputs to the ACC largely originate from the Bi and ABmc, preferentially connect to the sgACC, and that a subset collaterally project to both sgACC and pgACC. These findings advance our understanding of fear extinction and fear expression circuitry across species.

Brain Activation Patterns in Response to Conspecific and Heterospecific Social Acoustic Signals in Female Plainfin Midshipman Fish, Porichthys notatus

While the peripheral auditory system of fish has been well studied, less is known about how the fish’s brain and central auditory system process complex social acoustic signals. The plainfin midshipman fish, Porichthys notatus, has become a good species for investigating the neural basis of acoustic communication because the production and reception of acoustic signals is paramount for this species’ reproductive success. Nesting males produce long-duration advertisement calls that females detect and localize among the noise in the intertidal zone to successfully find mates and spawn. How female midshipman are able to discriminate male advertisement calls from environmental noise and other acoustic stimuli is unknown. Using the immediate early gene product cFos as a marker for neural activity, we quantified neural activation of the ascending auditory pathway in female midshipman exposed to conspecific advertisement calls, heterospecific white seabass calls, or ambient environment noise. We hypothesized that auditory hindbrain nuclei would be activated by general acoustic stimuli (ambient noise and other biotic acoustic stimuli) whereas auditory neurons in the midbrain and forebrain would be selectively activated by conspecific advertisement calls. We show that neural activation in two regions of the auditory hindbrain, i.e., the rostral intermediate division of the descending octaval nucleus and the ventral division of the secondary octaval nucleus, did not differ via cFos immunoreactive (cFos-ir) activity when exposed to different acoustic stimuli. In contrast, female midshipman exposed to conspecific advertisement calls showed greater cFos-ir in the nucleus centralis of the midbrain torus semicircularis compared to fish exposed only to ambient noise. No difference in cFos-ir was observed in the torus semicircularis of animals exposed to conspecific versus heterospecific calls. However, cFos-ir was greater in two forebrain structures that receive auditory input, i.e., the central posterior nucleus of the thalamus and the anterior tuberal hypothalamus, when exposed to conspecific calls versus either ambient noise or heterospecific calls. Our results suggest that higher-order neurons in the female midshipman midbrain torus semicircularis, thalamic central posterior nucleus, and hypothalamic anterior tuberal nucleus may be necessary for the discrimination of complex social acoustic signals. Furthermore, neurons in the central posterior and anterior tuberal nuclei are differentially activated by exposure to conspecific versus other acoustic stimuli.

Koreas Language Studies Grammar Effect Relationship Analysis of the Chosun Language Textbooks as a High School Grammar Language Curriculum in the Intermediate Division Multilateral Context -Centered on Sentence Unit Grammar Education Contents

이 논문에서는 조선어문 선택 교과로서의 교과서와 남북한 문법교과서와의 비교 분석을 통해 조선어 문법 교과서에 나타난 남북한의 국어문법의 영향 관계를 파악해 보고자 한다. 이를 위해 조선어 고등 문법 교과서와 남북한 문법 교과서를 연구 대상으로 삼아 문장 단위 문법 교육 내용을 중심으로 비교분석하였다. 그 결과, 조선어 고등 교과서에 나타난 문장 단위 교육 내용 특징으로 1) 구어중심, 2) 의미 중심, 3) 양태 중심, 4) 문장 단위 중심을 들 수 있었다. 그러나 조선어 문법 교과서에 나타난 남북한의 국어 문법의 영향 관계를 파악하기 위해서는 이전의 교육과정 변화에 따라 문법 교과서 기술이 어떠한 방식으로 이루어져 왔는지에 대한 사적 고찰이 뒷받침될 필요가 있다. 이와 관련한 논의가 활성화될 수 있기를 기대해 본다.

Optimising feed-in tariff design through efficient risk allocation

Abstract Many Feed-in Tariff designs exist. This paper provides a framework to determine the optimal design choice through an efficient allocation of market price risk. Feed-in Tariffs (FiTs) incentivise the deployment of renewable energy technologies by subsidising remuneration and transferring market price risk from investors, through policymakers, to a counterparty. This counterparty is often the electricity consumer. Using Stackelberg game theory, we contextualise the application of different FiT policy designs that efficiently divide market price risk between investors and consumers, conditional on risk preferences and market conditions. Explicit consideration of policymaker/consumer risk burden has not been incorporated in FiT analyses to date. We present a simulation-based modelling framework to carry this out. Through an Irish case study, we find that commonly employed flat-rate FiTs are only optimal when policymaker risk aversion is extremely low whilst constant premium policies are only optimal when investor risk aversion is extremely low. When both policymakers and investors are risk averse, an intermediate division of risk is optimal. We provide evidence to suggest that the contextual application of many FiT structures is suboptimal, assuming both investors and policymakers are at least moderately risk averse. Efficient risk allocation in FiT design choice will be of increasing policy importance as renewables deployment grows.

The role of cell location and spatial gradients in the evolutionary dynamics of colon and intestinal crypts.

BackgroundColon and intestinal crypts serve as an important model system for adult stem cell proliferation and differentiation. We develop a spatial stochastic model to study the rate of somatic evolution in a normal crypt, focusing on the production of two-hit mutants that inactivate a tumor suppressor gene. We investigate the effect of cell division pattern along the crypt on mutant production, assuming that the division rate of each cell depends on its location.ResultsWe find that higher probability of division at the bottom of the crypt, where the stem cells are located, leads to a higher rate of double-hit mutant production. The optimal case for delaying mutations occurs when most of the cell divisions happen at the top of the crypt. We further consider an optimization problem where the “evolutionary” penalty for double-hit mutant generation is complemented with a “functional” penalty that assures that fully differentiated cells at the top of the crypt cannot divide.ConclusionThe trade-off between the two types of objectives leads to the selection of an intermediate division pattern, where the cells in the middle of the crypt divide with the highest rate. This matches the pattern of cell divisions obtained experimentally in murine crypts.ReviewersThis article was reviewed by David Axelrod (nominated by an Editorial Board member, Marek Kimmel), Yang Kuang and Anna Marciniak-Czochra. For the full reviews, please go to the Reviewers’ comments section.Electronic supplementary materialThe online version of this article (doi:10.1186/s13062-016-0141-6) contains supplementary material, which is available to authorized users.

Messy but meaningful: exploring the transition to reform-based pedagogy with teachers of mathematics and coordinators in Ontario, Canada

The RE4MUL8 Project involved the creation of an online/mobile resource for Intermediate Division (Grade 7 and 8) teachers of mathematics. This resource showcases video documentaries of seven key mathematics topic lessons (fractions, integers, proportional reasoning, composite shapes and solids, solving equations, and, patterning and algebraic thinking), as delivered by seven teachers in Ontario, Canada who were nominated by their respective District School Boards as being, or becoming, highly effective practitioners in the area of reform-based mathematics education. As part of a qualitative case study research design, these teachers, often along with their math coordinators, were then interviewed following the lesson, and shared reflections on the lesson itself and, more generally, on their ongoing journey towards reform-based mathematics teaching. This paper reports on three major themes that emerged from these discussions, namely, problem-based learning, the reality and necessity of ‘messy time’ transition to reform-based pedagogy, and, balancing instructional planning and practices.

Hummocky cross-stratification-like structures and combined-flow ripples in the Punta Negra Formation (Lower-Middle Devonian, Argentine Precordillera): A turbiditic deep-water or storm-dominated prodelta inner-shelf system?

Turbidity-current and storm-induced deposits may exhibit similarities, in particularly when the latter is laid down by a combination of oscillatory and unidirectional flows. Recent progress in facies analysis helps to discriminate the sedimentary effects of oscillatory from unidirectional components of the flow. On the basis of detailed analysis of sedimentary facies, strata geometry, and palaeocurrent data, the present study reinterprets the Punta Negra Formation (PNF) (Lower-Middle Devonian, Argentine Precordillera), previously considered as a depositional system of deep-water, as a storm-dominated prodeltaic shelf depositional system.In the sandstone beds of the PNF, planar, low-angle and undulating laminations with weakly asymmetric hummocky and swaley bedforms, combined-flow ripples, accretionary hummocky cross-stratification-like (HCS-like), and anisotropic HCS-like suggest the action of oscillatory currents combined with unidirectional currents in forming the deposits. Different hypotheses on the origin of the oscillatory currents have been examined. The most convincing interpretation is that the oscillatory component of the velocity is attributed to storm-induced waves. The palaeocurrent data indicate offshore current directions, suggesting that the unidirectional flow was a gravity-induced bottom current. Inverse grading at the base and overlying normally graded divisions of the sandstone beds testify to waxing–waning behaviour of the depositional flows; interbedding of sedimentary structures (undulating laminations, low-angle and parallel laminations, and combined-flow ripples) in the lower and intermediate divisions of the beds indicate fluctuations of flow velocity. This organisation of the sedimentary structures permits association of the unidirectional component with hyperpycnal bottom currents. The terrestrial origin of the hyperpycnal flows is suggested by the abundance of terrestrial plant remains, the mineralogical and textural immaturity of the sandstone composition, and the relative scarcity of bioturbation, which was likely controlled by fresh-water input and a high rate of sedimentation. Storm-influenced, hyperpycnal flows generated subaqueous channelised forms at the mouth of the river deltas, which later filled with sand. At the distal end of the channelised forms, lobe-shaped sandstone beds were deposited, evolving distally into thin sandstone beds alternating with sandy mudstone.

Accounting Boot Camp

I explore the effectiveness of a program designed to improve student effort and performance in intermediate accounting and other upper division accounting courses. At a mid-sized public university in the Western US, many students enrolled in Intermediate Accounting I (their first upper division course) struggled with the rigor and academic demands of this course, resulting in failure rates of 30%-45%. Most retake the course and ultimately contribute to nationally competitive CPA pass rates. Additionally, in other upper division accounting courses, students new to the upper division underperformed their more experienced peers. In both cases, students new to upper division courses appeared to lack a commitment to, or awareness of, the effort needed for success in the accounting program. An accounting “Boot Camp” was implemented to prepare new accounting majors for the expectations of the program. I detail the implementation of the boot camp and review its effectiveness by comparing the performance of attendees with non-attendees. Controlling for other contributing factors, I find evidence that attendees took their studies more seriously and outperformed their non-attending peers in Intermediate Accounting I. Attendees in other upper division accounting courses no longer underperformed their more experienced peers. As an alternative to entrance exams or extensive review sessions, this expectations-oriented boot camp solution may help students transition from lower-division to upper-division courses in a variety of majors.

Vision-based judgment of tomato maturity under growth conditions

To determine the picking time of tomato and design the control strategy for the harvesting robot, the judgment of tomato maturity under natural conditions is required. Tomato samples were collected based on the fruit growth conditions which were divided into five different stages in this article: breakers, turning, pink, light-red, and red stages. The visible CCD camera VS-880HC was adopted to shoot visible images, while the near-infrared images at a wavelength of 810 nm were screened by MS- 3100 multi-spectral camera. The variations of samples, about color features, were analyzed. The tests indicated that with the changes in maturity, the hue-mean of tomato decreased and the red-green colordifference image mean increased. The standard deviations of the hue-mean and red-green image mean were the largest values for tomato in the pink stage, but the intensity mean of the near-infrared image for tomato in the pink stage had the lowest value. Hue-mean and red-green color-difference image mean can be used as a criterion for the judgment of tomato maturity, and the tests indicated that the redgreen mean method was more satisfactory than that of the hue-mean in the maturity recognition methods of tomato fruit with an accuracy of over 96%. The intermediate divisions of five different maturity stages, which were divided by red-green color-difference image mean, were 0, 23.5, 42.5 and 70. The judgment errors of the two methods are mainly caused by the recognition of tomatoes at the pink stage. Key words : Tomato, maturity, image, judgment.

Multiple forebrain systems converge on motor neurons innervating the thyroarytenoid muscle

The present study investigated the central connections of motor neurons innervating the thyroarytenoid laryngeal muscle that is active in swallowing, respiration and vocalization. In both intact and sympathectomized rats, the pseudorabies virus (PRV) was inoculated into the muscle. After initial infection of laryngomotor neurons in the ipsilateral loose division of the nucleus ambiguus (NA) by 3 days post-inoculation, PRV spread to the ipsilateral compact portion of the NA, the central and intermediate divisions of the nucleus tractus solitarii, the Botzinger complex, and the parvicellular reticular formation by 4 days. Infection was subsequently expanded to include the ipsilateral granular and dysgranular parietal insular cortex, the ipsilateral medial division of the central nucleus of the amygdala, the lateral, paraventricular, ventrolateral and medial preoptic nuclei of the hypothalamus (generally bilaterally), the lateral periaqueductal gray, the A7 and oral and caudal pontine nuclei. At the latest time points sampled post-inoculation (5 days), infected neurons were identified in the ipsilateral agranular insular cortex, the caudal parietal insular cortex, the anterior cingulate cortex, and the contralateral motor cortex. In the amygdala, infection had spread to the lateral central nucleus and the parvicellular portion of the basolateral nucleus. Hypothalamic infection was largely characterized by an increase in the number of infected cells in earlier infected regions though the posterior, dorsomedial, tuberomammillary and mammillary nuclei contained infected cells. Comparison with previous connectional data suggests PRV followed three interconnected systems originating in the forebrain; a bilateral system including the ventral anterior cingulate cortex, periaqueductal gray and ventral respiratory group; an ipsilateral system involving the parietal insular cortex, central nucleus of the amygdala and parvicellular reticular formation, and a minor contralateral system originating in motor cortex. Hypothalamic innervation involved several functionally specific nuclei. Overall, the data imply complex CNS control over the multi-functional thyroarytenoid muscle.

Finite depth and Jacobson–Bourbaki correspondence

We introduce a notion of depth three tower C ⊆ B ⊆ A with depth two ring extension A | B being the case B = C . If A = End B C and B | C is a Frobenius extension with A | B | C depth three, then A | C is depth two. If A , B and C correspond to a tower G > H > K via group algebras over a base ring F , the depth three condition is the condition that K has normal closure K G contained in H . For a depth three tower of rings, a pre-Galois theory for the ring End A C B and coring ( A ⊗ B A ) C involving Morita context bimodules and left coideal subrings is applied to specialize a Jacobson–Bourbaki correspondence theorem for augmented rings to depth two extensions with depth three intermediate division rings.