Various methods to evaluate the biologic reactions of the gingiva and the oral mucosa to dental materials are discussed. Examples and results are given of tests in subgingival cavity preparations, tests in buccal pouches, and tests in contact to the palatal mucosa for short, intermediate, and prolonged contact periods between the material and the mucosa. Proved testing appliances are described. The results, compared with those of toxicity screening tests, indicate the need for an alternate applied test.
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