Intercropped Maize Research Articles

SYSTEM PRODUCTIVITY AND ECONOMICS OF INTERCROPPING Maize WITH SUGARCANE

To investigate the effects of intercropping of maize with sugarcane on cane development, outturn, and overall economic return, field experiments were carried out during the cropping year 2021–22 in two different locations: Bangladesh Sugarcrop Research Institute (BSRI), Ishwardi, Pabna and the Regional Sugarcrop Research Station (RSRS), Thakurgaon, Bangladesh. There were nine treatments namely T1 : Single row sugarcane only (SS), T2 : SS+ Maize (During sett placement), T3 : SS+ Maize (7 days after sett placement), T4 : SS+ Maize (14 days after sett placement), T5 : SS+ Maize (21 days after sett placement), T6 : SS+ Maize (28 days after sett placement), T7 : SS+ Maize (35 days after sett placement), T8 : SS+ Maize (42 days after sett placement), T9 : Maize (M) only. Sugarcane variety “Isd 39” and maize variety “Super 92” were used in this experiment as experimental materials. Results indicated that T7 treatment was superior in terms of germination (56.56% at BSRI and 57.33% at Thakurgaon). The treatments T1 (174.33×103 ha-1 and 184.00×103 ha-1) and T7 (162.67×103 ha-1 and 168.00×103 ha-1) consistently exhibited superior tiller production and millable cane yield at both the locations. However, T1 generally resulted in higher cane yield, while T7 demonstrated higher SEY (108.25 t ha-1 at BSRI and 114.79 t ha-1 at Thakurgaon), LER (1.56 at BSRI and 1.52 at Thakurgaon), ATER (1.20 at BSRI and 1.16 at Thakurgaon), gross monetary value (487133 BDT ha-1 at BSRI and 516533 BDTha-1at Thakurgaon), and monetary advantage index (175178 at BSRI and 177542 at Thakurgaon) compared to other treatments. Treatment T2 also showed competitive SEY (105.52 t ha-1 at BSRI and 108.69 t ha-1 at Thakurgaon), LER (1.64 at BSRI and 1.54 at Thakurgaon), ATER (1.13 at BSRI and 1.07 at Thakurgaon), gross monetary value (474867 BDT ha-1at BSRI and 489100 BDT ha-1at Thakurgaon), and monetary advantage index (184942 at BSRI and 173134 at Thakurgaon), indicating its effectiveness in enhancing sugarcane production. Therefore, it maize planted 35 days after sugarcane sett placement (T7 ) or during sugarcane sett placement (T2 ), profitable intercropping system.

Intercropping of Maize and Cowpea for Enhancing Productivity, Profitability and Land use Efficiency

A field experiment was conducted at agriculture farm, ICAR-Central Institute for Research on Goats (CIRG), Makhdoom, Mathura, Uttar Pradesh during kharif season of 2020. The treatments consist of sole maize, sole cowpea; and intercropping of maize + cowpea in 1 : 1, 2 : 1, 1 : 2, 2 : 2, 3 : 1, 1 : 3 and 3 : 3 row ratios. The results revealed that maximum total green fodder yield, maize and cowpea equivalent yield, Land Equivalent Ratio (LER), Monitory Advantage Index (MAI), net returns and Benefit: Cost ratio were recorded with 2 : 1 row ratio of maize + cowpea intercropping combination followed by 3 : 1 row ratio. The highest value of nitrogen and potassium content both in fodder maize and cowpea were recorded with 1 : 3 row ratios of maize + cowpea intercropping combination. Further, the highest phosphorus content in fodder maize was recorded with 3 : 1 row ratio of maize + cowpea intercropping combination whereas in case of cowpea the highest phosphorus content was recorded with 1 : 3 row ratio of maize + cowpea intercropping combination. Thus, it was found that two rows maize + one row of cowpea (2 : 1) intercropping combination perform best in terms of yield, land use efficiency and profitability of fodder maize and cowpea. Bangladesh J. Bot. 53(2): 235-242, 2024 (June)

Intercropping in maize reduces fall armyworm Spodoptera frugiperda (J. E. Smith) infestation, supports natural enemies, and enhances yield

Fall armyworm (FAW) is a multi-generational invasive insect pest of maize. Field experiments were conducted in diverse ecologies at Hyderabad, Telangana (Location 1); Dholi, Bihar (Location 2); Kolhapur, Maharashtra (Location 3) during the rainy season from July-November, 2023 to assess the impact of maize intercropping with legumes and leafy vegetables on FAW damage, abundance of beneficial insects, weed dynamics, and yield. In the present study, location-specific intercrops were selected for the experimentation. In location 1, maize intercropped with cowpea followed by groundnut reduced the level of FAW infestation compared to sole maize. The increased natural enemy population (coccinellids, spiders, earwigs); weed suppression, and higher yields were observed in maize when intercropped with cowpea followed by groundnut and red amaranthus. In location 2, maize intercropped with cowpea followed by green gram and black gram reduced the FAW damage and higher natural enemy population. Maximum yield was obtained in maize intercropped with black gram followed by green gram and cowpea. However, concerning weed suppression and cost-benefit ratio, green gram followed by black gram was found to be superior. Minimum FAW infestation, abundant natural enemy population, weed suppression, and improved economic returns were observed in maize when intercropped with cowpea followed by groundnut and fenugreek in location 3. Each intercrop had its advantages concerning pests, weed suppression, an abundance of natural enemy populations, and higher yields. Therefore, it is suggested that the selection of plants as intercrops with maize should be based on the location-specific importance of particular aspects to attain multifunctional benefits. The present results revealed that crop diversification with location-specific intercrops can reduce significant yield losses caused by FAW and promote higher yields in maize.

Border row effects improved the spatial distributions of maize and peanut roots in an intercropping system, associated with improved yield.

Border row effects impact the ecosystem functions of intercropping systems, with high direct interactions between neighboring row crops in light, water, and nutrients. However, previous studies have mostly focused on aboveground, whereas the effects of intercropping on the spatial distribution of the root system are poorly understood. Field experiments and planting box experiments were combined to explore the yield, dry matter accumulation, and spatial distribution of root morphological indexes, such as root length density (RLD), root surface area density (RSAD), specific root length (SRL), and root diameter (RD), of maize and peanut and interspecific interactions at different soil depths in an intercropping system. In the field experiments, the yield of intercropped maize significantly increased by 33.45%; however, the yield of intercropped peanut significantly decreased by 13.40%. The land equivalent ratio (LER) of the maize-peanut intercropping system was greater than 1, and the advantage of intercropping was significant. Maize was highly competitive (A = 0.94, CR=1.54), and the yield advantage is mainly attributed to maize. Intercropped maize had higher RLD, RSAD, and SRL than sole maize, and intercropped peanut had lower RLD, RSAD, and SRL than sole peanut. In the interspecific interaction zone, the increase in RLD, RSAD, SRL, and RD of intercropped maize was greater than that of intercropped peanut, and maize showed greater root morphological plasticity than peanut. A random forest model determined that RSAD significantly impacted yield at 15-60 cm, while SRL had a significant impact at 30-60 cm. Structural equation modeling revealed that root morphology indicators had a greater effect on yield at 30-45 cm, with interactions between indicators being more pronounced at this depth. These results show that border-row effects mediate the plasticity of root morphology, which could enhance resource use and increase productivity. Therefore, selecting optimal intercropping species and developing sustainable intercropping production systems is of great significance.

Maize and legume intercropping enhanced crop growth and soil carbon and nutrient cycling through regulating soil enzyme activities

Maize intercropped with leguminous green manure (LGM) has been proven as a sustainable plantation practice for enhancing crop yield and soil fertility. However, a comprehensive understanding of how intercropped legumes coordinate the above- and below-ground performance during maize growth under low to high N application remains elusive. This study aimed to investigate the effects of biological C and N input on soil fertility and maize growth by regulating soil enzyme activities in maize/LGM intercropping systems. A three-year field trial was conducted in northwestern China, where maize was intercropped with two LGMs, namely common vetch and pea, under no N (N0, 0 kg ha−1) and conventional N (N330, 330 kg ha−1) applications. The grain yield of intercropped maize ranged from 10.54 to 11.08 t ha−1, representing a significant increase of 6.6–12.1 % compared to monoculture maize in the N0 treatment. Nitrogen application substantially increased maize grain yield by 39.2–52.0 % across cropping systems relative to the N0 treatments, while minor differences were observed in maize yield between cropping systems in the N330 treatments. Compared with monoculture maize, intercropped with LGMs increased C input by 16.7–79.2 % at maize V9 stage and 81.1–140.3 % at the R6 stage. The biological N fixation of intercropped LGMs was 62.7–89.5 kg ha−1 and 8.0–12.8 kg ha−1 in the N0 and N330 treatments, respectively. Biological C and N input greatly facilitated soil enzyme activities and the associated nutrient cycling, consequently improving soil fertility. Soil organic matter and total N in the intercropping systems significantly increased by 4.4–14.3 % relative to monoculture across maize growth stages, irrespective of N levels. Furthermore, increased soil fertility was closely associated with nutrient stoichiometry, which in turn facilitated maize nutrient uptake and shoot recovery growth in the intercropping systems. Overall, these findings highlight that increased biological C and N input to belowground enhances soil C and nutrient cycling by regulating the involved enzyme activities, which in turn improves maize nutrient uptake and growth, forming a coordinated loop of C and nutrient flow in the maize and legume intercropping systems. Maize intercropped with LGMs is a sustainable practice that improves soil fertility and promotes maize growth by augmenting biological C and N input.

Effect of Intercropping Spinach and Radish on Growth and Yield of Maize (Zea mays)

Background: Intercropping increases crop yield per unit area by intensifying the use of land. Cultivation of maize with leafy vegetables provides better use of land and other environmental resources, which results in higher economic yield. The current investigation aimed to study the effect of maize-spinach and maize-radish intercropping on growth and yield of maize under rainfed condition of Namsai district of Arunachal Pradesh. Methods: The field experiment was conducted during 2021 in randomized block design consisting of 3 treatments i.e. T1- Sole Maize, T2- Maize + spinach and T3- Maize + radish with four replications. Observations on growth and yield parameters and yield of maize were recorded on harvesting of the crop. The results were analysed using standard statistical procedures of ANOVA. Result: In maize + spinach and maize + radish intercropping, plant height (176.3 cm), numbers of leaves (15.49) and leaf area (5407 cm2 plant-1) were significantly greater in sole maize than the intercropping system. On the other hand, the yield parameters as well as yield of maize was higher in maize + radish intercropping as compared to maize + spinach and sole maize system. The grain yield of maize under maize + radish intercropping was 3066.25 kg ha-1. The B:C (1.92) was significantly higher in intercrops than the sole maize crop. From this study, it can be concluded that the intercrops are agronomically and economically viable than sole cropping. The intercropping of maize with radish would be profitable due to higher yield of maize as well as B:C (2 rows of maize and 2 rows of soybean) under farmer’s field condition of Namsai district of Arunachal Pradesh.

Maize//Soybean Intercropping Improves Yield Stability and Sustainability in Red Soil under Different Phosphate Application Rates in Southwest China

Studying the effects of maize and soybean intercropping for improving the maize yield and sustaining stability of the maize yield under different phosphate (P) application rates in red soil is crucial for promoting maize productivity, improving soil fertility and optimizing P nutrient management in southwest China. The objective of this study was to evaluate the dynamic changes in maize yield, yield stability and soil fertility under monoculture and intercropping maize with different P application rates. A six-year field experiment was conducted from 2017 to 2022 to investigate the effects of maize intercropping with soybean on the yield stability and sustainability of maize according to the changes in the maize yield, biomass, partial land equivalent ratio of yield (pLERY), actual yield loss index (AYL), contribution rate of soil capacity and fertilizer (SCR, SFCR) over time, as well as the differences in the coefficient of variation (CV) and sustainable yield index (SYI) at four P application rates (0 kg P2O5 ha−1, P0; 60 kg P2O5 ha−1, P1; 90 kg P2O5 ha−1, P2; and 120 kg P2O5 ha−1, P3) based on the two-factor randomized block design. The linear-platform model was utilized to simulate the relationship between the grain yield, the SYI and the amount of P fertilizer under different P application rates. The maize yield in intercropping was significantly superior to the maize yield in monoculture throughout the entire six-year experiment. For all planting years, the yield and biomass of the intercropping were higher than those of the matched monoculture average by 56.0% and 56.1%, respectively. Intercropping had an advantage of pLERY and AYL for maize. Otherwise, intercropping reduced the CV by 30.8% and 39.1% and increased the SYI by 39.4% and 23.0% in P0 and P3 compared with the matched monoculture, respectively. For all planting years, the average SFCR in intercropping treatment was higher than that in monoculture treatment. The linear-plateau model fitted showed that intercropping increased the yield and SYI by 19.8% and 40.7% on the platform and reduced the P application rate by 37.8% and 11.9% at the inflection point, respectively. These results demonstrate that maize and soybean intercropping could achieve a higher yield, a higher yield stability and an SYI with a lower P input than monoculture. Maize and soybean intercropping could be a sustainable practice for promoting the maize productivity and the yield sustainability in the red soil of southwest China.

Plant spatial configurations and their influences on phenological traits of cereal and legume crops under maize‐based intercropping systems

AbstractIntroductionIntercropping systems have a great potential for crop diversification thus increasing smallholder systems' resilience to climate change while improving soil health. However, optimal benefits associated with intercropping systems are rarely realised because of the interspecific competition for growth resources among the intercropped species.MethodologySix trials were established in the high and low rainfall agroecological zones of Babati district in Tanzania to assess how promising cropping systems with different plant spatial configurations would influence the phenological development of intercropped maize, bean and pigeonpea. Cropping systems under study included a sole maize system rotated with a pigeonpea‐bean intercrop dubbed Doubled‐up legume (DUL), maize‐pigeonpea system both with and without de‐topping, an innovation comprising double maize rows alternated with pigeonpea and beans (Mbili‐Mbili), maize‐pigeonpea system with two maize seeds sown within a 50 cm intra‐row space, a vertical‐architecture Meru H513‐pigeonpea system and a farmer practice.ResultsBranch formation was significantly higher in DUL than in maize‐based systems (p ≤ 0.05). Seasonal weather had upto 30% influence on pigeonpea flowering, with DUL having highest (p ≤ 0.05) flower production. The rate of pigeonpea branch and flower production in Mbili‐Mbili was stable across seasons relative to other maize‐pigeonpea systems. Doubled‐up legume and farmer practice had pigeonpea litter yield of between 1 and 2 t ha−1 which was at least 0.5 t ha−1 higher than in maize‐based systems (p ≤ 0.05). During the period preceding early maize reproductive stages, Mbili‐Mbili increased light interception by 30% and 63% compared to maize‐based systems and DUL, respectively. Maize toppings had higher (94%) P content than stover biomass that remained until harvest.ConclusionOverall, maize‐legume systems had higher intercropping efficacy than sole maize system, both in interception use efficiency, soil mulch cover, among other soil health benefits. Mbili‐Mbili and DUL also had increased phenological benefits on intercropped legumes however, the latter was prone to seasonal weather variability.

Maize/Soybean Intercropping with Straw Return Increases Crop Yield by Influencing the Biological Characteristics of Soil.

With mounting demand for high-quality agricultural products and the relentless exploitation of arable land resources, finding sustainable ways to safely cultivate food crops is becoming ever more important. Here, we investigated the effects of the integrated cropping technique "straw return + intercropping" on the soil aggregates as well as the microbial biomass carbon (MBC) content, enzyme activities and microbial diversity in soils of maize and soybean crops. Our results show that in comparison to straw removal and monoculture, straw return and intercropping increase the rhizosphere's MBC content (59.10%) of soil, along with urease (47.82%), sucrase (57.14%), catalase (16.14%) and acid phosphatase (40.66%) activities as well as the microbial diversity under maize and soybean. Under the same straw treatment, the yield of maize when intercropped surpassed that when grown in monoculture, with the land equivalent ratio of the intercropping treatment under straw return being highest. Overall, the intercropping of maize and soybean is beneficial for the healthy development of sustainable agriculture in the black soil region of northeast China, especially when combined with straw return to fields.

Maize/soybean intercropping increases nutrient uptake, crop yield and modifies soil physio-chemical characteristics and enzymatic activities in the subtropical humid region based in Southwest China

Intercropping, a widely adopted agricultural practice worldwide, aims to increase crop yield, enhance plant nutrient uptake, and optimize the utilization of natural resources, contributing to sustainable farming practices on a global scale. However, the underlying changes in soil physio-chemical characteristics and enzymatic activities, which contribute to crop yield and nutrient uptake in the intercropping systems are largely unknown. Consequently, a two-year (2021–2022) field experiment was conducted on the maize/soybean intercropping practices with/without nitrogen (N) fertilization (i.e., N0; 0 N kg ha−1 and N1; 225 N kg ha−1 for maize and 100 N kg ha−1 for soybean ) to know whether such cropping system can improve the nutrients uptake and crop yields, soil physio-chemical characteristics, and soil enzymes, which ultimately results in enhanced crop yield. The results revealed that maize intercropping treatments (i.e., N0MI and N1MI) had higher crop yield, biomass dry matter, and 1000-grain weight of maize than mono-cropping treatments (i.e., N0MM, and N1MM). Nonetheless, these parameters were optimized in N1MI treatments in both years. For instance, N1MI produced the maximum grain yield (10,105 and 11,705 kg ha−1), biomass dry matter (13,893 and 14,093 kg ha−1), and 1000-grain weight (420 and 449 g) of maize in the year 2021 and 2022, respectively. Conversely, soybean intercropping treatments (i.e., N0SI and N1SI) reduced such yield parameters for soybean. Also, the land equivalent ratio (LER) and land equivalent ratio for N fertilization (LERN) values were always greater than 1, showing the intercropping system’s benefits in terms of yield and improved resource usage. Moreover, maize intercropping treatments (i.e., N0MI and N1MI) and soybean intercropping treatments (i.e., N0SI and N1SI) significantly (p < 0.05) enhanced the nutrient uptake (i.e., N, P, K, Ca, Fe, and Zn) of maize and soybean, however, these nutrients uptakes were more prominent in N1MI and N1SI treatments of maize and soybean, respectively in both years (2021 and 2022) compared with their mono-cropping treatments. Similarly, maize-soybean intercropping treatments (i.e., N0MSI and N1MSI) significantly (p < 0.05) improved the soil-based N, P, K, NH4, NO3, and soil organic matter, but, reduced the soil pH. Such maize-soybean intercropping treatments also improved the soil enzymatic activities such as protease (PT), sucrose (SC), acid phosphatase (AP), urease (UE), and catalase (CT) activities. This indicates that maize-soybean intercropping could potentially contribute to higher and better crop yield, enhanced plant nutrient uptake, improved soil nutrient pool, physio-chemical characteristics, and related soil enzymatic activities. Thus, preferring intercropping to mono-cropping could be a preferable choice for ecologically viable agricultural development.

Effects of Maize/Peanut Intercropping and Nitrogen Fertilizer Application on Soil Fungal Community Structure

Maize/peanut intercropping may improve soil health through reducing nitrogen (N) fertilization. However, the effects of maize/peanut intercropping combined with reduced N fertilization on the soil fungal community structure have not been well reported. Using a long-term localized micro-zone experiment, we investigated the combined effects of intercropping and N fertilizer application on soil fungal community diversity and composition. Three cropping patterns (maize/peanut intercropping, maize monoculture, and peanut monoculture) and three N application levels (0 kg·hm−2, 150 kg·hm−2, and 300 kg·hm−2) were assessed. The results showed that the total numbers of fungal species and unique species (operational taxonomic units, OTUs) in both maize and peanut soils tended to first increase and then decrease with increasing N application. Compared with monoculture, the numbers of total OTUs and unique OTUs in intercropped maize soil decreased by 4.14% and 12.79%, respectively, but the total numbers of OTUs and unique OTUs in peanut soil increased by 1.08% and 3.78%, respectively. With increasing N application, the soil fungal Ace and Chao indices of maize soil first increased and then decreased, while the fungal Shannon, Ace, and Chao indices of peanut soil decreased. Compared with the monoculture system, intercropping significantly reduced the maize soil fungal Ace and Chao indices but increased the peanut soil fungal Shannon, Ace, and Chao indices. Nitrogen application and intercropping significantly altered the fungal community structure of maize soil, while N application had no significant effect on the fungal community structure of peanut soil, though intercropping significantly changed the fungal community structure of peanut soil. At the phylum level, Ascomycota, Basidiomycota, Mortierellomycota, unclassified_k_Fungi, and Chytridiomycota were the dominant taxa. Redundancy analysis (RDA) showed that soil nitrate (NO3−) content was the main environmental factor shaping the soil fungal community. In conclusion, excessive N fertilization (300 kg·hm−2) can reduce soil fungal community diversity; maize/peanut intercropping reversed the negative effect of N application on fungal community of peanut soil, but not that of maize soil. Soil NO3− content is the primary environmental driver of soil fungal communities.

Effects of intercropping and regulated deficit irrigation on the yield, water and land resource utilization, and economic benefits of forage maize in arid region of Northwest China

Intercropping has been widely recognized to have great advantages in terms of increasing yield, controlling pests and diseases, and saving land, particularly in developing countries. Regulated deficit irrigation reduces water consumption and improves water productivity (WP). However, it is unclear whether the combination of intercropping and deficit irrigation could improve crop yield and WP simultaneously. In this experiment, three planting modes, including forage maize (Zea mays L.) monoculture (M), lablab bean (Lablab purpureus L.) monoculture (L), and maize-lablab bean intercropping (ML) were used. Six irrigation modes were set for each planting mode, including severe water deficit (W1), late water deficit (W2), alternate water deficit (W3), late moderate water deficit (W4), early moderate water deficit (W5), and full irrigation (W6). Results showed that compared with M, the ML treatment significantly increased the fresh forage yield (9.8%–17.0%), hay yield (9.5%–13.1%), crude protein yield (22.9%–25.9%), and WP (7.8%–8.7%). The W5 treatment achieved similar fresh forage yield, hay yield, and crude protein yield as that of the W6 treatment but reduced irrigation water by 25% and increased the WP (21.9%–24.8%). Intercropping achieved a high-water equivalence ratio (WER;1.52–1.81) and land equivalence ratio (LER;1.56–1.84), indicating its advantages over monocultures. The W6 treatment had the lowest WER and LER, suggesting that excessive irrigation can reduce the efficiency of utilizing land and water resource in maze-based forage production. Among all treatments, ML–W5 achieved the highest net income and output to input ratio. Overall, intercropping of forage maize and lablab bean with moderate deficit irrigation at an early stage could be used as a high-yield and efficient forage production system in the arid areas of northwest China.

Influence of Pteris vittata-maize intercropping on plant agronomic parameters and soil arsenic remediation

To achieve “production while remediation” in arsenic (As) -contaminated farmlands, a field experiment was conducted to investigate the effects of five Pteris vittata L. (PV) - maize intercropping modes on the growth, nutrient, and As accumulation characteristics of PV and maize. The intercropping increased the As content of PV by 2.9%–132.0% and decreased the As content in maize shoots by 15.5%–37.0%. Total As accumulation in above-ground plant parts reached 202.03–941.97 g hm−2. Intercropping also improved nitrogen and phosphorus content in maize kernels by 27.6%–124.7% and 15.9%–31.5%, respectively. Additionally, intercropping increased maize kernel 100-grain weight by 10.0%–16.6% and resulted in a 1.1%–24.1% increase in maize yield compared to sole cultivation. The intercropping transformed soil As from iron-bound to calcium-bound and aluminum-bound forms. Analysis of soil microbial diversity showed that the intercropping decreases the abundance of Chloroflexi and increases the abundance of Proteobacteria. Among the five modes, the intercropping mode with 4 rows of maize and 4 rows of PV showed the highest remediation efficiency and mechanized operation. These findings contribute to a theoretical framework and technical support for the simultaneous soil pollution remediation and productive farming practices.

Effect of intercropping maize and sunn hemp at different times and stand densities on soil properties and crop yield under in-field rainwater harvesting (IRWH) tillage in semi-arid South Africa

Abstract Background Evidence suggests that manipulating intercropping timing and stand density within intercropping systems could enhance crop yields. However, our current understanding of the effects of intercropping a cover crop on soil chemical properties and moisture still needs to be improved. This study investigates the effects of intercropping sunn hemp with maize at different timings and stand densities on selected soil properties and crop yield. Materials and methods A split-plot experiment was conducted under the in-field rainwater harvesting (IRWH) tillage. The trial had three intercropping times (simultaneously with maize planting, at V15 maize growth stage, and R1 maize growth stage) as the main plot factors and three stand densities (16, 32, and 48 plants m−2) as the subplot factors, with three replicates for both the 2019/20 and 2020/21 seasons. Changes in soil properties were assessed within the uppermost layer (0-30 cm). Soil moisture content was continuously monitored throughout the growing season and specific soil chemical properties were analyzed at harvest. Results The results showed that the interaction of sunn hemp intercropping period and stand densities did not significantly influence most of the measured soil properties. The early planting of sunn hemp had significantly 32.4% higher soil organic matter (SOM) than the last planting date at low stand density. After two growing seasons SOM, nitrogen, potassium, and manganese were significantly enhanced by 39.7%, 19.0%, 21% and 60.6% respectively. However, during the same period calcium, sodium and iron were significantly reduced by 13.4%, 46.1% and 78.0% respectively. The management of sunn hemp crop had significant effect on maize grain yield across the two seasons. The maize yields in the medium and high stand densities in the first season were significantly 15.3% and 34.3% higher than in the second season, respectively. Conclusion Due to the intercropping treatments, the retention of sunn hemp residues with varying quantities and qualities may have influenced the soil nutrient dynamics in the short-term. Significant changes in soil chemical properties and yield may need more time, and future research should be conducted out in agricultural regions with different soil mineral matrices.

Nutrient management for enhancing productivity of pigeonpea (Cajanus cajan) based intercropping system under rainfed condition

A field experiment was conducted during the rainy (kharif) season of 2011–12 and 2012–13 at Tirhut College of Agriculture, Dholi, Bihar, to study the effect of nutrient management for enhancing productivity of pigeonpea [Cajanus cajan (L.) Millsp.]-based intercropping systems. Pigeonpea + turmeric (Curcuma longa L.) intercropping system, recorded significantly higher pigeonpea yield (2.00 t/ha), pigeonpea-equivalent yield (4.79 t/ha), fruiting efficiency (18.8%), water-use efficiency (6.1 kg grain/ha/mm), production efficiency (18.0 kg/ha/day) and net re turns ( 106.32 ×10/ha) than pigeonpea + maize (Zea mays L.) intercropping and sole pigeonpea. However, pigeonpea + turmeric recorded lower benefit: cost (B: C) ratio than sole pigeonpea. Significantly higher B: C ratio was recorded under pigeonpea + sesame (Sesamum indicum L.) (2.32) and pigeonpea + urdbean [Vigna mungo (L.) Hepper] (2.31) intercropping systems than pigeonpea + maize, pigeonpea + turmeric and sole pigeonpea. Pigeonpea + maize recorded significantly lower NPK uptake by pigeonpea than sole pigeonpea and the other in tercropping systems. Pigeonpea + mungbean [Vigna radiata (L.) R. Wilczek] and pigeonpea + urdbean recorded significantly higher number and dry weight of nodules /plant than pigeonpea + maize. Application of 125% recom mended dose of fertilizer (RDF) recorded significantly higher number of branches and pods/plant, pigeonpea equivalent yield (3.39 t/ha), land-equivalent ratio (1.87), production efficiency (12.8 kg/ha/day), net returns ( 80.83 ×103/ha), benefit: cost ratio (2.17) and NPK uptake by pigeonpea than RDF and 75% RDF. Pigeonpea equivalent yield in pigeonpea + urdbean, pigeonpea + mungbean and pigeonpea + sesame intercropping sys tems, increased significantly with the increasing levels of fertilizer up to RDF, and further increase in fertilizer level did not show significant effect on pigeonpea-equivalent yield. However, it increased significantly up to 125% RDF in pigeonpea + maize and pigeonpea + turmeric intercropping systems. Pigeonpea + urdbean and pigeonpea + mungbean increased organic carbon, available N, P, K content of the soil than the other intercropping systems and initial soil value.

Shifting focus from external to in situ organic resources – The redesign of four tropical long-term experiments

Long-term experiments (LTEs) are critical for evaluating strategies that can maintain or increase crop yields, soil fertility and soil organic carbon (SOC), and help adapt to climate change. Yet, scientific knowledge is advancing and research questions are evolving. Therefore, it is important to review the objectives of LTEs over time. A change in their design may be necessary to keep the experimental treatments scientifically interesting, innovative, and relevant in the context of evolving agricultural challenges. Here, we describe the process of redesigning four LTEs in Kenya. These LTEs are unique in that they represent four different pedoclimatic conditions but with identical experimental treatments across sites. Initially, they focused on investigating how to maintain or increase SOC and maize yields over time by applying a combination of mineral nitrogen (N) and external organic resources. Specifically, the experimental treatments consisted of maize monoculture with different rates (1.2 and 4 t C ha−1 yr−1) and qualities of organic resources, either with or without mineral N fertilizer input. After about 20 years, it became clear that SOC was lost in most treatments. Therefore, continuing with the current experimental design was not an option. Taking advantage of the fact that the different former treatments led to different levels of soil degradation, we redesigned the LTEs to study the effectiveness of regenerative cropping strategies in rebuilding SOC and increasing crop yields starting from the different levels of soil degradation. The focus shifted from external to in situ organic inputs by increasing the root biomass of the cultivated crops. The newly established cropping system treatments are maize-legume rotation, maize-legume intercropping (double row configuration) and relay intercropping of maize with forage grass. A key finding from the previous phase of the experiments, namely, that external organic inputs with low C:N ratios are most efficient in building SOC, has been incorporated into the redesign. The relative contribution of external versus in situ organic resources is tested by splitting the cropping system treatments into those receiving either farmyard manure or green manure in the form of Tithonia diversifolia prunings and those receiving no external inputs. Split-plot treatments with and without mineral N were retained. The overall objective of studying mechanisms of tropical soil fertility maintenance and, more specifically, SOC formation, remained unchanged. However, the redesign aligned the LTEs with the current state of knowledge and pressing research questions, specifically focusing on the relative effectiveness of in-situ versus external organic inputs in SOC formation.

Understanding the interactions of genotype with environment and management (G×E×M) to maize productivity in Conservation Agriculture systems of Malawi.

Climatic variability and soil fertility decline present a fundamental challenge for smallholder farmers to determine the optimum management practices in the production of maize. Optimizing genotype (G) and management (M) of maize under different environmental conditions (E) and their interactions are essential for enhancing maize productivity in the smallholder sector of Malawi where maize is the main staple food. Here, we evaluated over seven seasons, the performance of four commercial maize genotypes [including hybrids and one open pollinated variety (OPV)] managed under different Conservation Agriculture (CA) and conventional practices (CP) across on-farm communities of central and southern Malawi. Our results revealed significant G×E and E×M interactions and showed that hybrids such as DKC 80-53 and PAN 53 outyielded the other hybrid and the OPV in most of the environments while the OPV ZM523 had greater yields in environments with above-average rainfall and shorter in-season dry spells. These environments received a maximum of 1250 mm to 1500 mm of rainfall and yet the long-term averages were 855 mm and 1248 mm, respectively. Despite yielding lower, the OPV ZM523 also exhibited higher yield stability across environments compared to the hybrid MH 30, possibly due to its resilience to drought, heat stress, and low soil fertility conditions which are often prevalent in the target communities. Conservation Agriculture-based practices outyielded CP across the genotypes and environments. However, amongst the CA-based systems, intercropping of maize with pigeonpea [Cajanus cajan (L.) Millsp] and cowpea (Vigna unguiculata Walp.) performed less than monocropping maize and then rotating it with a legume probably due to competition for moisture between the main and the companion crops in the intercrop. The key findings of this study suggest the need to optimize varietal and management options for particular environments to maximize maize productivity in Malawi. This means that smallholder farmers in Malawi should adopt hybrids and CA-based systems for enhanced yields but could also consider OPVs where the climate is highly variable. Further rigorous analysis that includes more abiotic stress factors is recommended for a better understanding of yield response.

Enhancing intercropping sustainability: Manipulating soybean rhizosphere microbiome through cropping patterns

Understanding the responses of soybean rhizosphere and functional microbiomes in intercropping scenarios holds promise for optimizing nitrogen utilization in legume-based intercropping systems. This study investigated three cropping layouts under film mulching: sole soybean (S), soybean–maize intercropping in one row (IS), and soybean–maize intercropping in two rows (IIS), each subjected to two nitrogen levels: 110 kg N ha−1 (N110) and 180 kg N ha−1 (N180). Our findings reveal that cropping patterns alter bacterial and nifh communities, with approximately 5 % of soybean rhizosphere bacterial amplicon sequence variants (ASVs) and 42 % of rhizosphere nifh ASVs exhibiting altered abundances (termed sensitive ASVs). Root traits and soil properties shape these communities, with root traits exerting greater influence. Sensitive ASVs drive microbial co-occurrence networks and deterministic processes, predicting 85 % of yield variance and 78 % of partial factor productivity of nitrogen, respectively. These alterations impact bacterial and nifh diversity, complexity, stability, and deterministic processes in legume-based intercropping systems, enhancing performance in terms of yield, nitrogen utilization efficiency, land equivalent ratio, root nodule count, and nodule dry weight under IIS patterns with N110 compared to other treatments. Our findings underscore the importance of field management practices in shaping rhizosphere-sensitive ASVs, thereby altering microbial functions and ultimately impacting the productivity of legume-based intercropping systems. This mechanistic understanding of soybean rhizosphere microbial responses to intercropping patterns offers insights for sustainable intercropping enhancements through microbial manipulation.

Grain yield, water-land productivity and economic profit responses to row configuration in maize-soybean strip intercropping systems under drip fertigation in arid northwest China

Intercropping has great potential for alleviating arable land competition, improving land output and promoting sustainable agricultural development. However, the applicability of maize-soybean strip intercropping under drip fertigation in arid northwest China remains unclear, especially under various row configurations. A two-season (2022 and 2023) field experiment was performed in the Hexi Region of northwest China to investigate the responses of plant growth, yield performance, water-land productivity and economic profit of drip-fertigated maize-soybean strip intercropping systems to eight row configurations. The results showed that intercropping significantly reduced aboveground biomass accumulation of maize and soybean by 18.77% and 47.81% on average compared to monocropping, respectively. Intercropping significantly decreased the 100-grain weight, ear length and ear width of maize, and reduced the 100-grain weight and pod number of soybean, resulting in reduced grain yields of intercropped maize and soybean (by 13.08% and 48.73%, respectively), but two rows of maize alternating with four rows of soybean (M2S4), three rows of maize alternating with four rows of soybean (M3S4), four rows of maize in wide and narrow rows alternating with four rows of soybean (M4S4-MN), and four rows of maize in wide and narrow rows alternating with six rows of soybean (M4S6-MN) produced greater population grain yield compared to monocropping. Among all intercropping systems, the largest water-land productivity and economic profit occurred in M2S4 (1.61 in 2022 and 1.42 in 2023 for land equivalent ratio; 29.23 kg ha−1 mm−1 in 2022 and 28.22 kg ha−1 mm−1 in 2023 for water productivity; 23,965 CNY ha−1 in 2022 and 23,059 CNY ha−1 in 2023 for economic profit), followed by M4S4-MN (1.53 in 2022 and 1.36 in 2023 for land equivalent ratio; 27.11 kg ha−1 mm−1 in 2022 and 26.58 kg ha−1 mm−1 in 2023 for water productivity; 22,327 CNY ha−1 in 2022 and 22,224 CNY ha−1 in 2023 for economic profit). The M2S4 is thus the optimal row configuration for drip-fertigated maize-soybean strip intercropping systems in terms of grain yield, economic profit and land productivity, while the M4S4-MN is recommended by further considering the efficiency of mechanized sowing and harvesting.

Intercropping improves maize yield and nitrogen uptake by regulating nitrogen transformation and functional microbial abundance in rhizosphere soil

Intercropping-driven changes in nitrogen (N)-acquiring microbial genomes and functional expression regulate soil N availability and plant N uptake. However, present data seem to be limited to a specific community, obscuring the viewpoint of entire N-acquiring microbiomes and functions. Taking maize intercropped with legumes (peanut and soybean) and non-legumes (gingelly and sweet potato) as models, we studied the effects of intercropping on N transformations and N-acquiring microbiomes in rhizosphere soil across four maize growth stages. Meanwhile, we compiled promising strategies such as random forest analysis and structural equation model for the exploitation of the associations between microbe-driven N dynamics and soil-plant N trade-offs and maize productivity. Compared with monoculture, maize intercropping significantly increased the denitrification rate of rhizosphere soils across four maize growth stages, net N mineralization in the elongation and flowering stages, and the nitrification rate in the seedling and mature stages. The abundance of most N-acquiring microbial populations was influenced significantly by intercropping patterns and maize growth stages. Soil available N components (NH4+-N, NO3−-N, and dissolved organic N content) showed a highly direct effect on plant N uptake, which mainly mediated by N transformations (denitrification rate) and N-acquiring populations (amoB, nirK3, and hzsB genes). Overall, the adaptation of N-acquiring microbiomes to changing rhizosphere micro-environments caused by intercropping patterns and maize development could promote soil N transformations and dynamics to meet demand of maize for N nutrient. This would offer another unique perspective to manage the benefits of the highly N-effective and production-effective intercropping ecosystems.