Intercropped Silages Research Articles

Produção integrada de silagem de Urochloabrizantha e silagem de milho ou sorgo: avaliação agronômica, perdas de fermentação e estabilidade aeróbia da silagem

ABSTRACT: The present study evaluated germination, production, and morphological composition of Urochloabrizantha intercropped with corn and sorghum; and silage fermentation losses and aerobic stability of intercrop silage using microbial inoculant. Twenty experimental parcels (5.0 × 3.6 m) were used in a blocked randomized design to evaluate four treatments obtained from a 2 × 2 factorial arrangements: I) crop material (corn vs. sorghum) and II) Brachiaria (U. brizantha) establishment (present vs. absent). Corn- and sorghum-brachiaria integrated systems showed similar brachiaria germination, forage yield, and morphological composition. There was no crop and brachiaria interaction effect on the variables related to corn and sorghum plants and the total productivity. Brachiaria decreased the stem diameter and increased the population of maize and sorghum plants. However, it did not affect systems productivity. Microbial inoculation did not affect corn silage effluent losses and reduced sorghum silage effluent losses. In corn silage, brachiaria did not affect gas losses, while in sorghum silage, brachiaria increased the gas losses. Total losses were higher in sorghum silage than in corn silage, which resulted in a lower DM recovery. The treatments did not affect the pH of the silage after aerobic exposure. However, brachiaria increased silage temperature evaluated at 32 and 40 hours after aerobic exposure. Thus, corn or sorghum consortium has similar brachiaria morphological composition and productivity. Moreover, in intercropped silage, brachiaria increases effluent losses and reduces silage aerobic stability.

Influence of Lactobacillus plantarum inoculation on the silage quality of intercropped Lablab purpureus and sweet sorghum grown in saline-alkaline region.

Ensiling legume with cereal is an effective method to ensure the energy rich-feed, but no information is available on the microbial fermentation mechanism of intercropped Lablab purpureus (Lablab) and sweet sorghum in the saline-alkaline region. Therefore, the present study investigated the silage quality and microbial community of intercropped Lablab and sweet sorghum silages grown in the saline-alkaline region with or without inoculation of Lactobacillus plantarum (LP). The experimental treatments were prepared according to the Lablab and sweet sorghum planting patterns: Lablab and sweet sorghum sowing seed ratios were 1:1 (L), 5:1 (M), and 9:1 (H). After harvesting, each mixture was treated with LP or sterilized water (CK), followed by 60 days of fermentation. Results showed that both LP inoculation and intercropping significantly raised the lactic acid (LA) content and decreased the pH value, acetic acid (AA), and ammonia-N in intercropped silages. The LP addition and intercropping also improved the relative feed value by reducing structural carbohydrates. Moreover, LP silages had a greater relative abundance of Lactobacillus than CK silages, and its relative abundance increased with an increased seed-sowing ratio of Lablab in intercropping. LP was the prevalent species in LP silages compared to CK silages, and its relative abundance also increased with an increased seed-sowing ratio of Lablab in intercropping. The genus Lactobacillus was negatively correlated with ammonia-N (R = -0.6, p = 0.02) and AA (R = -0.7, p < 0.01) and positively correlated with LA (R = 0.7, p < 0.01) and crude protein (R = 0.6, p = 0.04). Overall, the intercropped seeding ratios of Lablab and sweet sorghum of ≥ 5:1 with LP inoculation resulted in better fermentation quality and preservation of nutritional components providing theoretical support and guidance for future intercropped protein-rich silage production in the saline-alkaline region.

Legume Integration Augments the Forage Productivity and Quality in Maize-Based System in the Loess Plateau Region

The changing climate, inadequate water supply, insufficient agricultural inputs, decreasing in agricultural arable land areas under forage crops of Northwestern Loess Plateau region, expanding livestock population, increasing demands for meat and milk production, and food and feed security concerns all insist on a necessary requirement in forage quality production. Cereal–legume mixed-cropping is a biological approach to enhancing herbage yield and quality of upgraded animal feed (forage and silage). However, little information exists about the appropriate mixing seeding ratios and its impacts on yield and quality. Therefore, this study was conducted to examine the forage yield and nutritional quality of maize (Zea mays L.) and common bean (Phaseolus vulgaris L.) in mono-and mixed-cropping approaches at the seeding proportions of 100:0, 75:25, 50:50, 45:55, 25:75, and 0:100 in 2019 and 2020 in Northwestern Loess Plateau region. A randomized complete block design with four replicates was used in this experiment. The results indicated that forage quality was significantly affected by the mixture ratios. The land equivalent ratio (LER) of all mixed-cropping treatments greater than 1.0, in which maize–common bean at the 50:50 seeding ratio achieved higher LER (1.46) than that of other treatments, showing that mixed-cropping combination systems are better users of land resources. Laboratory forage quality analysis and Pearson correlation analysis showed that the relative feed value had highly positive correlation with total digestible nutrients and relative forage quality in mixed-cropping treatments. Our results showed that fresh forage yield and dry matter yield were higher in monocropped maize forage than in other intercropped forages, whereas crude protein yield was lower compared with other mixed cropping forages. After 60 days of ensiling, the highest organic acid profile and ammonia-nitrogen were observed in M25:CB75 silage compared with other silages. The highest ensilability of fermentation coefficient was also found in M50:CB50 compared with other intercropped silages. Regarding forage preservation, silage showed higher contents of crude protein, relative feed value and lower crude fiber, water-soluble carbohydrate neutral detergent fiber, and acid detergent fiber contents than forage. This study determined that the ratios of maize–common bean 25:75 and 50:50 were the most desirable mixture ratios among mixed-cropped forage and silage based on chemical composition and quality analysis for livestock feeding.

Maize-soybean intercrop silage yield and quality with different nitrogen levels and plant population

This study hypothesizes that maize-soybean intercrop with lower maize plant population and nitrogen levels can allow better soybean development and yield, which may offset lower maize biomass and silage crude protein yield. Experiment was carried out in a randomized complete block design arranged in a 2 x 5 factorial scheme, with three replications. Treatments consisted of two different maize-soybean plant stand (40,000 and 60,000 plants ha-1) and five nitrogen fertilization levels (0, 50, 100, 150, and 200 kg ha-1). As a result, soybean biomass yield increased at the lower maize plant stand, although, maize and total (maize + soybean) biomass yield were higher at the greater maize plant stand. Thus, individual maize plants and total dry matter yield increased as nitrogen levels were increased. However, there was no effect of the studied factors on the silage crude protein yield per area, indicating a great potential of soybean to offset biomass yield reduction trough silage quality improvement. Moreover, since there was no difference on total silage crude protein yield per hectare, it is suggested that the adoption of maize-soybean intercrop with lower maize plant stand (40 thousand maize plants ha-1) and with lower nitrogen values is a more environmentally friendly approach to increase farmland sustainability while decreasing environmental and productivity costs. Intermediate levels may be evaluated in future studies

Evaluation of Nutritive Values through Comparison of Forage Yield and Silage Quality of Mono-Cropped and Intercropped Maize-Soybean Harvested at Two Maturity Stages

Maize and soybean intercropping is a cereal-legume intercropping pattern that not only increases grain yield but also improves the nutritional value of silage. Experiments were conducted in the summer season to compare the yield and nutritional composition of the forage and silage quality of mono-cropped maize and intercropped maize-soybean harvested at two stages of maturity. The main treatments were one sole crop maize (SM) and four maize-soybean intercropping patterns (one-row maize to one-row soybean (1M1S), one-row maize to two-row soybean (1M2S), one-row maize to three-row soybean (1M3S), and two-row maize to one-row soybean (2M1S). The crops were harvested when the maize reached the milk (R3) and maturity (R6) stages. Results indicated a significant increase in the fresh biomass and dry-matter production of maize fodder alone compared with those of maize intercropped with soybean fodder. After 60 days of ensiling period, silage samples were analyzed for pH, organic acids, dry matter, crude protein (CP), ether extract, neutral detergent fiber (NDF), acid detergent fiber (ADF), and other mineral compositions. All intercropped silages showed higher CP values (1M1S, 12.1%; 1M2S, 12.2%; 1M3S, 12.4%; and 2M1S, 12.1%) than the SM silage (8.7%). Higher organic acids were produced in 1M3S than in the other silages. Correlation data showed that CP was highly correlated with lactic acid but negatively associated with crude fiber, nitrogen-free extract, and NDF. Thus, the intercropping of maize and soybean silage is recommended due its enhanced crop production, nutritional values in dairy animals, and prolific animal feedings and because it was scientifically evaluated as a feed stuff. This study indicated that 1M3S was the most preferable among intercropped silages in terms of nutrient composition.

Does intercropping maize with forage sorghum effect biomass yield, silage bromatological quality and economic viability?

Maize and forage sorghum crops have a relevant economic importance in Midwest Brazil and there is a need for more information for their intercropping as a strategy for the farmers to reduce inputs. The objective of this work is evaluate if forage sorghum and maize intercropping effects agronomic traits, the bromatological characteristics of silage from the consortium at different times of silo openings, and the economic viability of all crop systems. This experiment was carried on in the field with seven treatments, consisting of monoculture and intercropping of corn and sorghum hybrids. Monoculture of sorghum Agri 001 and maize Agri 320, as well as in maize Agri 320, Agri 340 and Agri 104 intercropped with sorghum Agri 001 had the highest biomass yield. Intercropped maize did not have lodging, reduction in height, nor decrease in the first ear height in relation to the intercropped plants. Maize hybrids intercropped with sorghum led to a better fermentation pattern of silages, reducing ammonia concentrations. However, due the higher contents of NDF and the lower TDN contents of sorghum, nutritional values of intercropped silages were generally smaller than when they were composed only with maize. It was observed that the monoculture of sorghum Agri 001 for silage showed a higher profit compared to the other treatments.

Faba bean-rapeseed silage as substitute for Italian ryegrass silage: effects on performance and milk quality of grazing dairy cows

Three trials were conducted to study the effect of including in the ration of dairy cows the intercrop faba bean-rapeseed or Italian ryegrass silages on feed intake, milk quality and enteric methane emissions. Ten lactating Holstein cows, randomly allocated in two groups, were used in each trial in a crossover design. Dry matter intake and milk yield were recorded daily for each data period. Milk was sampled for analyses three times per period. Enteric methane emission was estimated applying a model that includes bodyweight, dry matter intake, nutritional values of feeds and milk composition. Concentrate and grass intake did not differ between treatments, while total mixed ration intake was higher with Italian ryegrass silage. Milk yield and protein concentration also increased with the Italian ryegrass treatment. The milk urea concentration was higher with the faba bean and rapeseed diet. Fatty acid profiles were affected by feeding strategy. Thereby, t11 18:1, c9 18:1 and total unsaturated fatty acid were higher when the intercrop silage was fed. As consequence, atherogenicity index and thrombogenicity index were lower under that treatment. Estimated enteric methane emissions did not differ between treatments. In conclusion, faba bean-rapeseed intercrop can be an alternative to Italian ryegrass to feed dairy cows. The rations formulated with the intercrop silage have potential to improve the milk fatty acid content, and show healthier profiles for consumers. However, it would be useful to study the protein content of these crops to avoid possible excess urea in milk and to maintain sustainable milk yield and milk protein content.

Forage Yield and Silage Quality of Intercropped Maize+Soybean With Different Relative Maturity Cycle

The success of maize+soybean intercrop depends on the correct synchronism between species phenological stages at the silage point. Due to it, the experiment was carried out to evaluate maize+soybean intercrop forage yield and silage quality using crops with different maturity cycle combination. The experiment used a randomized complete block design with a 2 &amp;times; 3 factorial scheme. Treatments consisted of two maize hybrids (1: P1630YHR-early cycle and 2: middle cycle P30F53VYHR) and two soybean cultivars (P95R51-maturity cycle of 5.1; TMG7062-maturity cycle of 6.2) and one control represented by maize monocrop. Silage harvesting was performed when maize had reached 2/3 milk line stage. Intercropping soybean into maize did not affect its biomass yield. Both soybean cultivars present compatible cycles for ensiling together with maize hybrids, since they were in phenological stages from R5.3 to R7 by the time maize was at its optimum stage for ensiling. There was interaction between species for the soybean biomass yield. Maize hybrid P30F53 produced higher biomass yield than P1630 what also resulted in higher amount of total crude protein yield. Intercrop P1630-P95R51 produced 458 Kg ha-1 of crude protein more than maize monocrop. Maize+soybean intercropping system results in higher silage crude protein percentage and yield per area (Kg of CP ha-1).

Effects of Different Planting Pattern of Maize (&amp;lt;i&amp;gt;Zea mays&amp;lt;/i&amp;gt; L.) and Soybean (&amp;lt;i&amp;gt;Glycine max&amp;lt;/i&amp;gt; (L.) Merrill) Intercropping in Resource Consumption on Fodder Yield, and Silage Quality

An experiment was carried out at the field units of the north campus experimental areas in Northwest Agriculture and Forestry University, Yangling, Shaanxi Province, P. R. China. The experiment was conducted on summer season (June to September) to determine the effects of different planting patterns of maize and soybean intercropping in resource consumption on fodder yield and silage quality. The main treatments were one sole crop of maize (SM) and four maize-soybean intercropping patterns (1 row maize to 1 row soybean (1M1S), 1 row maize to 2 rows soybean (1M2S), 1 rows maize to 3 rows soybean (1M3S) and 2 rows maize to 1 row soybean (2M1S), respectively. The experiment was a randomized complete block design with three replications, and plot size of 12 m by 5 m. The crops were harvested when the maize reached at milk stage and soybean at R7 stage. The result indicated significant increase in fresh biomass and dry matter production of maize fodder alone as compared to maize intercropped with soybean fodder. It was correlated with a higher consumption of environmental resources, such as photosynthetically active radiation (PAR) and soil moisture by intercropping. After 45 days of ensiling period, silage samples were analyzed for pH, organic acids (Lactic, acetic, and butyric), dry matter (DM), crude protein (CP), ether extract (EE), neutral detergent fibre (NDF), acid detergent fibre (ADF), calcium (Ca), sodium (Na), phosphorus (P), magnesium (Mg), and potassium (K). It was concluded that in all intercropped silages, crude protein (CP) values were higher (1M1S, 12.1%; 1M2S, 12.2%; 1M3S, 12.4%; 2M1S, 12.1%) than the monocrop maize (SM, 8.7%) silage. Higher organic acids (p < 0.05) were produced in the 1M3S silages as compared to others silages. The study indicated that among all intercropped silages, the 1M3S (1 row maize to 3 rows soybean) was preferable according to nutrient composition than other intercropped silages.

Effect of Different Planting Structure of Maize and Soybean Intercropping on Fodder Production and Silage Quality

A study was conducted to examine the effects of different planting structure of maize (Zea maysL.) and soybean (Glycine max L.) intercropping on fodder production and silage quality.Maize was cultivated alone and intercropped with soybean as follows;1 row maize to 1 row soybean (1M1S),1 row maize to 2 rows soybean (1M2S) and 1 row maize to 3 rows soybean (1M3S).The experiment was laid out in randomized complete block design with four treatments and three replications.The crops were harvested when the maize reached at heading stage (at about 35% dry matter).The results indicated significant increase in fresh biomass and dry matter production of maize fodder alone as compared to maize intercropped with soybean fodder.However,no difference (p&gt;0.05) was observed in ether extract (EE ), ash (%)and acid detergent fibre (ADF) of nutrient composition of fodder among the four treatments.After 60 days of ensiling period,silage samples were analysed for pH,dry matter (DM),crude protein (CP),ether extract (EE),neutraldetergent fibre (NDF), acid detergent fibre (ADF) and water soluble carbohydrate (WSC).All intercropped silages had higher CP values (1M1S,12.1%;1M2S,12.1%;1M3S,12.7%) than the monocrop maize (SM,8.4%) silage.The NDF and ADF levels were higher for maize silage as compared to intercropped silages. The study showed that among all intercropped silages the IM3S (1 row maize to 3 rows soybean) was preferable according to nutrient composition than other intercropped silages.

Comparison of Yield, Nutritive Value, and In Vitro Digestibility of Monocrop and Intercropped Corn-Soybean Silages Cut at Two Maturity Stages

Limited information on nutrient composition and in vitro digestibility of corn-soybean intercropped silage is available. The objective of this study was to compare corn (Zea mays L.) or soybean (Glycine max L. Yesilsoy) monocrop silage with corn-soybean intercropped silages in term of yield, nutritive value, and in vitro digestibility. Intercropping was as follows: 1 row corn to 1 row soybean (1M1S), 1 row corn to 2 rows soybean (1M2S) and 2 rows corn to 1 row soybean (2M1S). The crops were harvested when the corn reached 3/5 or 1/4 milk line. The silage samples were analysed for pH, dry matter (DM), crude protein (CP), ether extract (EE), neutral (NDF) and acid detergent fibre (ADF), calcium, potassium, magnesium and phosphorus. Also, in vitro true (IVTD) and in vitro NDF (IVNFD) digestibilities were determined in the silages samples. The DM, EE, calculated non structural carbohydrate values were higher in silage harvested at 1/4 than 3/5 milk line. All intercropped silages had higher CP values (1M1S, 8.3%; 1M2S, 10.1%; 2M1S, 8.0%) than the monocrop corn (SM, 6.8%) silage. The NDF and ADF levels were higher for 1M1S, 2M1S and SM compared with 1M2S and monocrop soybean (SS) silage. In vitro true DM digestibility of all silages increased with maturity stage; it was higher for the 1M2S than other silages. It is concluded that corn-soybean intercropped silage has better nutrient composition and digestibility than SM or SS silage.

Replacing grass silage with pea-barley intercrop silage in the feeding of the dairy cow

The effect of replacing wilted grass silage (GS) with pea-barley intercrop silage (PBS) on feed intake, diet digestibility and milk production was studied with 8 multiparous Ayrshire-cows in a replicated 4 × 4 Latin square experiment. Proportion of PBS was 0 (PBS0), 33 (PBS33), 67 (PBS67) or 100 (PBS100) % of silage dry matter (DM). The DM content was 559 and 255 g kg-1 for GS and PBS. Crude protein content was 131 and 170 g kg-1 DM, respectively. Pea-barley silage was more extensively fermented than GS with total fermentation acid content of 120 vs. 12 g kg-1 DM. Silage was fed for ad libitum intake and supplemented with on the average 13 kg concentrate per day. Silage DM intake was 9.2 (PBS0), 9.7 (PBS33), 9.0 (PBS67) and 7.1 (PBS100) kg per day (Pquadr. &lt; 0.05). The energy corrected milk yield [30.3 (PBS0), 29.8 (PBS33), 30.3 (PBS67), 31.3 (PBS100) kg per day] was not significantly affected by the treatment. Milk protein concentration decreased linearly (P &lt; 0.05) in response to feeding PBS. It is concluded that PBS can replace up to two thirds of wilted, moderate quality GS in the feeding of dairy cows because in this experiment pure pea-barley silage reduced silage intake.

Ruminal degradation characteristics of corn and corn–sunflower intercropped silages prepared at two stages of maturity

The degradation characteristics of dry matter (DM), protein, neutral detergent fiber (NDF) and acid detergent fiber (ADF) of corn–sunflower intercrop silage (CSS) and monoculture corn silage (CS) prepared from whole plants, harvested at two stages of maturity, were compared using eight mature rumen-cannulated steers. The degradation characteristics were determined by incubating the silages in nylon bags for up to 72 h in the rumen of animals fed the respective silages. The degradation characteristics determined for the silages were the soluble fraction, the insoluble but degradable fractions, the rate of degradation of the degradable fractions of silage DM and protein, and the in situ disappearance of NDF and ADF after 0.5 and 72 h of incubation. The rate of particulate passage from the rumen was determined using chromium-mordanted NDF of the four silages. Values were used to estimate effectively degraded DM and protein. The rates of DM and protein degradation were highest for late-cut CSS (6.3 and 6.0% h−1, respectively) and the least for late-cut CS (2.5 and 0.8% h−1, respectively). Averaged across stages of maturity, more (P &lt; 0.05) DM and protein were effectively degraded with CSS (57.4 and 70.1%, respectively) than with CS (48.8 and 48.7%). Degradation of NDF in early-cut CSS was lower (P &lt; 0.05) than in CS after 72 h of incubation. ADF disappearance from all of the silages after 72 h of incubation was similar. Ruminal degradation of DM and protein in CSS was greater than in CS, which may affect efficiency of utilization of CSS. Key words: Degradation rate, effective degradability, corn silage, intercropped corn–sunflower, steers