Interconnected Loop Research Articles

Efficient Ethernet Ring Mesh Network Design

Ethernet ring protection (ERP) is an emerging solution for carrier-grade optical Ethernet networking developed as an ITU-T recommendation. The most recent development of the ERP technique provides multi-ring protection in a mesh packet transport network. The ERP configuration and capacity planning in a ring mesh topology can be optimized in the selection of the interconnected ring hierarchy and logical loop prevention block positions. This paper reports the first investigation to understand the designing principles of protected Ethernet ring mesh networks and proposes an optimized design scheme for cost-effective and reliable ERP networking.

Selection of Steam Drum Level Control Method for Multiple Drum Interacting Loops Pressure Tube-Type BWR

Three element Steam Drum (SD) Level Controller has been conventionally used for most of the boilers, Nuclear power plant steam generator & Boiling Water Reactor (BWRs). Based on the process dynamic studies it was found that this scheme does not work properly for an interacting, interconnected multiple loop boiling water system i.e., Advanced Heavy Water Reactor (AHWR). It is a pressure tube type light water cooled heavy water moderated Boiling Water Reactor (BWR). It has 4-inter-connected parallel loops with 113 × 4 = 452 boiling channels in the Main Heat Transport (MHT) system. These multiple (four) interconnected loops influences the Steam Drum (SD) level control adversely. Such a behavior has not been reported in the open literature. The open loop response is stable, non-oscillatory and non-diverging for a step change in the feed flow rates. Also it is not possible to maintain a steady level in all the SDs even without any external disturbance/perturbation with 4 conventional 3-element individual SD level controllers. To overcome these interactions it is proposed to interconnect all the four steam drums in the liquid & vapor regions respectively. This makes the 4 SDs behave like a single entity. The influence of the interconnect configuration & the level controller are studied in detail to find a robust solution. The response obtained for unsymmetrical core power, symmetrical power maneuvering and reactor trip transients shows that the SD levels do not diverge and quickly settle very near to the set points assigned with SD interconnect schemes.

The Function of the CLE Peptides in Plant Development and Plant-Microbe Interactions.

The CLAVATA3 (CLV3)/ENDOSPERM SURROUNDING REGION (ESR) (CLE) peptides consist of 12 or 13 amino acids, including hydroxylated proline residues that may or may not contain sugar modifications, and function in a non-cell-autonomous fashion. The CLE gene was first reported in Zea mays (maize) as an endosperm-specific gene, ESR, in 1997 (Opsahl-Ferstad et al., 1997). CLE genes encode secreted peptides that function in the extracellular space as intercellular signaling molecules and bind to cellular surface receptor-like proteins to transmit a signal. CLE peptides regulate various physiological and developmental processes and its signaling pathway are conserved in diverse land plants. Recent CLE functional studies have pointed to their significance in regulating meristematic activity in plant meristems, through the CLE-receptor kinase-WOX signaling node. CLV3 and CLE40 are responsible for maintenance of shoot apical meristem (SAM) and root apical meristem (RAM) function, regulating homeodomain transcription factors, WUSCHEL (WUS) and WUSCHEL-related homeobox 5 (WOX5), respectively. CLE and WOX form an interconnected and self-correcting feedback loop to provide robustness to stem cell homeostasis. CLE peptides are required for certain plant-microbe interactions, such as those that occur during legume symbiosis and phytopathogenic nematode infection. Understanding the molecular properties of CLE peptides may provide insight into plant cell-cell communication, and therefore also into plant-microbe interactions.

WUSCHEL mediates stem cell homeostasis by regulating stem cell number and patterns of cell division and differentiation of stem cell progenitors

Plant stem cell populations, unlike their animal counterparts, do not use cell migration and oriented cell divisions to maintain their size, and therefore require a precise coordination between self-renewing divisions of stem cells, and rates of cell division and differentiation among stem cell progenitors. Shoot apical meristems (SAMs) of higher plants harbor a set of stem cells within the central zone (CZ) that divide infrequently. Stem cell daughters that are displaced towards the surrounding peripheral zone (PZ) divide at a faster rate and enter into differentiation at specific locations to form leaves or flowers. The relative ratios of cells in the CZ and the PZ are maintained, despite a constant displacement of cells from the CZ into the PZ, and subsequent allocation of cells within the PZ to form organ primordia. The mechanisms that mediate this homeostatic balance are not well understood. A homeodomain transcription factor WUSCHEL, expressed in the rib meristem (RM), located beneath the CZ, has been shown to provide nonautonomous cues for stem cell specification. By employing transient spatial manipulation and live imaging, we show that an elevated level of WUS not only induces expansion of the CZ, but also results in increased cell division rates in cells of the PZ; conversely, decreases in WUS level lead to a smaller CZ and are associated with a reduction in cell division rate. Moreover, low levels of WUS lead to enlarged organ primordia, by elevating the responsiveness of the PZ cells to the plant hormone auxin. This reveals a function of WUS in mediating the balance between differentiating and non-differentiating cells of the PZ. Regulation of stem cell numbers, growth and differentiation patterns by a single transcription factor forms a interconnected and self-correcting feedback loop to provide robustness to stem cell homeostasis in a dynamic cellular environment.

WUSCHEL mediates stem cell homeostasis by regulating stem cell number and patterns of cell division and differentiation of stem cell progenitors

Plant stem cell populations, unlike their animal counterparts, do not use cell migration and oriented cell divisions to maintain their size, and therefore require a precise coordination between self-renewing divisions of stem cells, and rates of cell division and differentiation among stem cell progenitors. Shoot apical meristems (SAMs) of higher plants harbor a set of stem cells within the central zone (CZ) that divide infrequently. Stem cell daughters that are displaced towards the surrounding peripheral zone (PZ) divide at a faster rate and enter into differentiation at specific locations to form leaves or flowers. The relative ratios of cells in the CZ and the PZ are maintained, despite a constant displacement of cells from the CZ into the PZ, and subsequent allocation of cells within the PZ to form organ primordia. The mechanisms that mediate this homeostatic balance are not well understood. A homeodomain transcription factor WUSCHEL, expressed in the rib meristem (RM), located beneath the CZ, has been shown to provide nonautonomous cues for stem cell specification. By employing transient spatial manipulation and live imaging, we show that an elevated level of WUS not only induces expansion of the CZ, but also results in increased cell division rates in cells of the PZ; conversely, decreases in WUS level lead to a smaller CZ and are associated with a reduction in cell division rate. Moreover, low levels of WUS lead to enlarged organ primordia, by elevating the responsiveness of the PZ cells to the plant hormone auxin. This reveals a function of WUS in mediating the balance between differentiating and non-differentiating cells of the PZ. Regulation of stem cell numbers, growth and differentiation patterns by a single transcription factor forms a interconnected and self-correcting feedback loop to provide robustness to stem cell homeostasis in a dynamic cellular environment.

Posttranslational Regulation ofNeurosporaCircadian Clock by CK1a-dependent Phosphorylation

Frequency (FRQ) and its transcriptional activator, the White Collar Complex (WCC), are essential components of interconnected feedback loops of the circadian clock of Neurospora. In a negative feedback loop, FRQ inhibits the WCC by recruiting casein kinase 1a (CK1a) and supporting its phosphorylation. In an interconnected positive loop, FRQ supports accumulation of high levels of WCC. Phosphorylation of clock proteins is crucial for the temporal and spatial coordination of these functions. We identified three isoforms of CK1a generated by alternative splicing that all interact with FRQ. Furthermore, we show that WC-2 is phosphorylated by CK1a in vitro and that WC-2 phosphorylation is inhibited in vivo by the CK1-specific inhibitor IC261. Finally, we demonstrate that CK1a activity regulates levels of WC-2.

Flux Analysis Uncovers Key Role of Functional Redundancy in Formaldehyde Metabolism

Genome-scale analysis of predicted metabolic pathways has revealed the common occurrence of apparent redundancy for specific functional units, or metabolic modules. In many cases, mutation analysis does not resolve function, and instead, direct experimental analysis of metabolic flux under changing conditions is necessary. In order to use genome sequences to build models of cellular function, it is important to define function for such apparently redundant systems. Here we describe direct flux measurements to determine the role of redundancy in three modules involved in formaldehyde assimilation and dissimilation in a bacterium growing on methanol. A combination of deuterium and 14C labeling was used to measure the flux through each of the branches of metabolism for growth on methanol during transitions into and out of methylotrophy. The cells were found to differentially partition formaldehyde among the three modules depending on the flux of methanol into the cell. A dynamic mathematical model demonstrated that the kinetic constants of the enzymes involved are sufficient to account for this phenomenon. We demonstrate the role of redundancy in formaldehyde metabolism and have uncovered a new paradigm for coping with toxic, high-flux metabolic intermediates: a dynamic, interconnected metabolic loop.

Nitrification by immobilized cells in a micro‐ecological life support system using packed‐bed bioreactors: an engineering study

AbstractThe nitrifying component of a micro‐ecological life support system alternative (MELISSA) based on microorganisms and higher plants was studied. The MELISSA system consists of an interconnected loop of bioreactors to allow the recycling of the organic wastes generated in a closed environment. Conversion of ammonia into nitrates in such a system was improved by selection of microorganisms, immobilization techniques, reactor type and operation conditions. An axenic mixed culture of Nitrosomonas europaea and Nitrobacter winogradskyi, immobilized by surface attachment on polystyrene beads, was used for nitrification in packed‐bed reactors at both bench and pilot scale. Hydrodynamics, mass transfer and nitrification capacity of the reactors were analysed. Mixing and mass transfer rate were enhanced by recirculation of the liquid phase and aeration flow‐rate, achieving a liquid flow distribution close to a well‐mixed tank and without oxygen limitation for standard operational conditions of the nitrifying unit. Ammonium conversion ranged from 95 to 100% when the oxygen concentration was maintained above 80% of saturation. The maximum surface removal rates were measured as 1.91 gN‐NH4+ m−2 day−1 at pilot scale and 1.77 gN‐NH4+ m−2 day−1 at bench scale. Successful scale‐up of a packed‐bed bioreactor has been carried out. Good stability and reproducibility were observed for more than 400 days. Copyright © 2004 Society of Chemical Industry

Modelling the dynamic performance of a construction organization

The Asian financial crisis has adversely affected many of the domestic construction organizations in Malaysia, bringing some organizations to the brink of insolvency. It demonstrates that a construction organization's performance is sensitive to the prevailing economic conditions. This paper seeks to model the dynamic performance of a publicly listed construction organization in Malaysia. First, a diagrammatic model consisting of two interconnected causal loop diagrams is developed to represent the organization's capacity and its financial balance sheet. The diagrams provide a clearer understanding of the interactions between the country's construction market and the organization's financial, technical and managerial capabilities. Next, the causal loop diagrams are converted into a mathematical model using specialized software. Simulation results from the model are validated against historical time series data. Graphical plots of the simulation model and results from sensitivity analysis indicate that the model is capable of replicating the general behaviour of the organization.

Application of Large Phase-Shifting Transformer on an Interconnected System Loop

A GROUP of 132-kv and 66-kv transmission systems form a loop about 300 miles in circumference and connect many important generating stations and a number of load points in the western part of Pennsylvania and the eastern portion of Ohio.

Application of large phase-shifting transformer on an interconnected system loop

A GROUP of 132-kv and 66-kv transmission systems form a loop about 300 miles in circumference and connect many important generating stations and a number of load points in the western part of Pennsylvania and the eastern portion of Ohio.

Carrier Relaying and Rapid Reclosing at 110 Kv

This paper describes an application of carrier-current relaying combined with rapid reclosure of circuit breakers by means of which the continuity of service to a large industrial load supplied by a long single-circuit line was improved. A 110-kv line 120 miles long, forming part of an interconnected loop, has been equipped in this way to clear faults and restore service in approximately one second. The effect of the connected motor load on circuit interruption is also discussed.