Interaction Of Microplastics Research Articles

Polystyrene microplastics attenuated the impact of perfluorobutanoic acid on Chlorella sorokiniana: Hetero-aggregation, bioavailability, physiology, and transcriptomics.

Microplastics (MPs) and perfluorobutanoic acid (PFBA), emerging contaminants, are ubiquitous in the environment and toxic to organisms. The interaction of MPs with other contaminants can affect their toxicity. However, the impact of MPs on PFBA toxicity remains unknown. This research investigated the individual and combined impacts of polystyrene microplastics (PS) and PFBA to Chlorella sorokiniana based on cellular responses and transcriptome analyses. Results showed that both PS and PFBA induced excessive ROS generation, causing cell membrane damage and photosynthesis inhibition, ultimately inhibiting Chlorella sorokiniana growth. The highest inhibition was observed for small particle size PS, with a maximum inhibition rate of 15.98 ± 1.24 % (3PS group). Notably, the combined toxicity of PFBA and PS exhibited antagonism, which was attributed to PFBA adsorption and PS hetero-aggregation via massive EPS secretion, resulting in decreased PS and PFBA bioavailability. Transcriptomic analysis elucidated that EPS secretion was promoted owing to the up-regulation of carbohydrate, amino acid, and energy metabolism (carbon fixation, oxidative phosphorylation, and TCA cycle). Enhanced EPS secretion, ABC transport, and antioxidant response alleviated PS and PFBA toxicity. Our findings offer valuable data for the joint ecotoxicity of PFASs and MPs, contributing to environmental risk assessment of co-pollution.

Toxicity effects of microplastics individually and in combination with Aeromonas hydrophila on freshwater crayfish (Astacus leptodactylous).

Opportunistic pathogens, such as Aeromonas hydrophila, can cause damage to freshwater crayfish (Astacus leptodactylous) in some situations. In addition to direct damage to the body, microplastics (MPs) can also be responsible for transmitting pathogens to the animal. Accordingly, this research was prepared to investigate the effects of MP on the damage caused by A. hydrophila exposure in A. leptodactylous. Hepatic oxidative biomarkers, blood biochemical indices, and clinical signs were investigated in freshwater crayfish co-exposed to MPs (500 and 1000 mg kg-1) and A. hydrophila (5 and 10% of the median lethal dose (LD50)) for 30 days. In the hemolymph of infested crayfish with A. hydrophila, there were no significant changes in glutathione peroxidase activity or total antioxidant level. However, in some of the clinical parameters, exposure to MP alone had a significant effect. Cholesterol levels, glutathione peroxidase, catalase, and the activity of γ-glutamyltransferase reduced, conversely, glucose, malondialdehyde, and aspartate aminotransferase increased. After co-exposure to A. hydrophila and MPs, certain parameters elevated in the hemolymph of crayfish such as glucose, activity of alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase. However, total protein, cholesterol, γ-glutamyltransferase, glutathione peroxidase, catalase activity, and total antioxidants decreased. In contrast, elevation in malondialdehyde content and superoxide dismutase activity was observed in the hepatocytes of crayfish after co-exposure to A. hydrophila and MPs. To summarize, the investigation demonstrated that the interaction of MPs and A. hydrophila in crayfish has a synergistic effect on various factors.

Microplastics from face masks: Unraveling combined toxicity with environmental hazards and their impacts on food safety.

Microplastics (MPs) refer to tiny plastic particles, typically smaller than 5mm in size. Due to increased mask usage during COVID-19, improper disposal has led to masks entering the environment and releasing MPs into the surroundings. MPs can absorb environmental hazards and transfer them to humans and animals via the food chain, yet their impacts on food safety and human health are largely neglected. This review summarizes the release process of MPs from face masks, influencing factors, and impacts on food safety. Highlights are given to the prevalence of MPs and their combined toxicities with other environmental hazards. Control strategies are also explored. The release of MPs from face masks is affected by environmental factors like pH, UV light, temperature, ionic strength, and weathering. Due to the chemical active surface and large surface area, MPs can act as vectors for heavy metals, toxins, pesticides, antibiotics and antibiotic resistance genes, and foodborne pathogens through different mechanisms, such as electrostatic interaction, precipitation, and bioaccumulation. After being adsorbed by MPs, the toxicity of these environmental hazards, such as oxidative stress, cell apoptosis, and disruption of metabolic energy levels, can be magnified. However, there is a lack of comprehensive research on both the combined toxicities of MPs and environmental hazards, as well as their corresponding control strategies. Future research should prioritize understanding the interaction of MPs with other hazards in the food chain, their combined toxicity, and integrating MPs detection and degradation methods with other hazards.

Membrane technology as a strategy for microplastics removal from landfill leachate: a review

ABSTRACT This study offers a comprehensive review of global microplastic (MP) contamination in landfill leachate (LL) and examines remediation strategies using membrane technologies such as ultrafiltration (UF), nanofiltration (NF), reverse osmosis (RO), and membrane bioreactors (MBRs). Research investigations and full-scale applications of these technologies for treating LL demonstrate their efficacy as viable solutions for on-site leachate treatment, providing promise in mitigating LL toxicity and reducing the environmental and human health risks associated with MP pollution. While the size of MPs in LL may raise questions about the necessity of using NF and RO membranes for MP removal, these processes are commonly employed in many landfills to serve as barriers for MP retention. Despite the high efficacy of MBR systems in removing MPs, the accumulation of MPs in the biological sludge can adversely affect biological performance and membrane fouling, necessitating further exploration. In general, membrane technologies face challenges such as membrane fouling and the release of MPs. Therefore, further research is needed to address MP removal, understand membrane–MP interactions, explore cleaning strategies in LL treatment and their impact on MP release from membranes, and study the integrity of membranes after continuous exposure to LL under varied operating conditions.

Polystyrene influence on Pb bioavailability and rhizosphere toxicity: Challenges for ramie (Boehmeria nivea L.) in soil phytoremediation

Microplastics (MPs) and heavy metals often coexist in soil, however their interactions and effects on the soil–plant system remain largely unclear. In this study, ramie (Boehmeria nivea L.) was exposed to soil contaminated with lead (Pb) and polystyrene (PS) of different sizes, dosages, and surface–charged functional groups. This design aimed to simulate the effects of MPs on phytoremediation. The experimental results revealed that PS exacerbated the damaging effects of Pb on ramie. Compared to the effect of Pb alone, PS–COOH had a greater influence on root vigor, leading to a 15.6 % reduction in the active absorption ratio. Laser scanning confocal microscope showed PS entered the roots. Adsorption/desorption experiments demonstrated that PS had a weaker adsorption capacity for Pb than soil but a greater desorption rate than soil when simulating rhizosphere secretion. Moreover, PS reduced soil pH and increased the reducible state of Pb by 6–12 %. After 100 days of phytoremediation, Pb content in the soil with PS–5 μm was 150 μg g−1 less than that in the soil without PS. These results demonstrated that PS improved Pb bioavailability and enhanced the efficiency of Pb uptake by ramie. The redundancy analysis demonstrated that PS mitigated the toxicity of Pb to rhizosphere microorganisms, potentially via its effects on metal chemical fractions, dehydrogenase activity (S–DHA), cation exchange capacity (CEC), and soil organic matter (SOM). This study indicates that the presence of PS could potentially enhance the phytoremediation efficiency of ramie in Pb–contaminated land by influencing soil microenvironmental properties. This study provides insights into the complex interactions of MPs with soil–plant–microbial systems during metal remediation, thereby enhancing our understanding of their environmental impacts.

Interaction of Micro- and Nanoplastics with Enzymes: The Case of Carbonic Anhydrase.

Microplastics (MPs) and nanoplastics (NPs) have emerged as significant environmental pollutants with potential detrimental effects on ecosystems and human health. Several studies indicate their interaction with enzymes; this topic represents a multifaceted research field encompassing several areas of interest from the toxicological and ecotoxicological impact of MPs and NPs on humans and wildlife to the biodegradation of plastics by microbial enzymes. This review aims to provide a critical analysis of the state-of-the-art knowledge of the interaction of MPs and NPs on the enzyme carbonic anhydrase (CA), providing recent insights, analyzing the knowledge gaps in the field, and drawing future perspectives of the research and its application. CA is a widespread and crucial enzyme in various organisms; it is critical for various physiological processes in animals, plants, and bacteria. It catalyzes the reversible hydration of CO2, which is essential for respiration, acid-base balance, pH homeostasis, ion transport, calcification, and photosynthesis. Studies demonstrate that MPs and NPs can inhibit CA activity with mechanisms including adsorption to the enzyme surface and subsequent conformational changes. In vitro and in silico studies highlight the role of electrostatic and hydrophobic interactions in these processes. In vivo studies present mixed results, which are influenced by factors like particle type, size, concentration, and organism type. Moreover, the potentiality of the esterase activity of CA for plastic degradation is discussed. The complexity of the interaction between CA and MPs/NPs underscores the need for further research to fully understand the ecological and health impacts of MPs and NPs on CA activity and expression and glimpses of the potentiality and perspectives in this field.

Microplastics and arsenic speciation in edible bivalves from the coast of China: Distribution, bioavailability, and human health risk

Bivalves, such as oysters and mussels, are exposed to environmental pollutants, like microplastics (MPs) and arsenic (As). This study investigated co-existence and interaction of MPs and As (total As and As species) in two bivalve species from the Chinese coastline. Smaller MPs (20–100 μm) averaged 30.98 items/g, while larger MPs (100–500 μm) averaged 2.98 items/g. Oysters contained more MPs (57.97 items/g) in comparison to mussels (11.10 items/g). In Contrast, mussels had a higher As concentrations (8.36–23.65 mg/kg) than oysters (4.97–11.02 mg/kg). The size and composition of MPs influenced As uptake and speciation in bivalves, with inorganic arsenic (iAs) and methylated arsenic (MMA and DMA) correlating with larger-sized MPs. Polyethylene (PE) may interact with the formation of arsenobetaine (AsB) in oyster. This study provides valuable insights into the interaction of MPs and As in marine ecosystems and highlights their implications for food safety.

Traversing the prevalence of microplastics in soil-agro ecosystems: Origin, occurrence, and pollutants synergies

The ubiquity of plastics in modern life has made them a significant environmental concern and a marker of the Anthropocene era. The degradation of plastics results in the formation of microplastics (MPs), which measure 5 mm or less. The coexistence of MPs with other pollutants found in sludge, water treatment plant effluents, surface water, and groundwater, shapes the environmental landscape together. Despite extensive investigation, the long-term implications of MPs in soils remain uncertain, underscoring the importance of delving into their transportation and interactions with soil biota and other contaminants. The present article provides a comprehensive overview of MPs contamination in soil, encompassing its sources, prevalence, features, and interactions with soil flora and fauna, heavy metals, and organic compounds. The sources of MPs in soil agroecosystems are mulching, composting, littering, sewage sludge, irrigation water, and fertilizer application. The concentration of MPs reported in plastic mulch, littering, and sewage sludge is 503 ± 2760 items per kg−1, 4483 ± 2315 MPs/kg, and 11,100 ± 570 per/kg. The transport of MPs in soil agroecosystems is due to their horizontal and vertical migration including biotic and abiotic mobility. The article also highlighted the analytical process, which includes sampling planning, collection, purification, extraction, and identification techniques of MPs in soil agroecosystems. The mechanism in the interaction of MPs and organic pollutants includes surface adsorption or adhesion cation bridging, hydrogen bonding, charge transfer, ligand exchange, van der Waals interactions, and ion exchange.

Aging and adsorption behavior of PP-MPs for methylene blue and tetracycline in unitary and binary systems

The omnipresence of microplastics (MPs) around the world has attracted extensive attention in the past decade with more focuses on the interactions of standard MPs without additives in regular shapes and individual pollutant, whereas the actual MPs containing various additives in irregular shapes and complex pollutants are often co-occurrence in the environments. In this paper, the adsorption performance of disposable polypropylene (PP) cups-based MPs subjected to ultraviolet irradiation was investigated in unitary and binary water matrices. The surface characteristics were analyzed and the experimental data of adsorption were fitted by various kinetic and isotherm models, and the results indicated that more cracks and oxygen-containing functional groups with decreased hydrophobicity were produced with aging, and electrostatic attraction and hydrogen bonding dominated methylene blue (MB) and tetracycline (TC) capture in the individual system. Moreover, pseudo-second order kinetic model better described the adsorption processes. In the binary system, the co-existence of TC promoted MB uptake, while the presence of MB inhibited TC capture. In addition, TC adsorption was enhanced by Ca2+, maybe due to its complexation effect, while the presence of mono- and divalent inorganic salts inhibited MB capture. This research provides useful insights for the fate of PP-MPs and organic pollutants in the complex environments.

In vitro digestion of microplastics in human digestive system: Insights into particle morphological changes and chemical leaching

Despite the well-reported occurrences and established pathways for microplastics (MPs) ingestion by humans, the eventual fate of these particles in the human gastrointestinal system is poorly understood. The present study tries to gain a better understanding of the fate of four common food-borne MPs, i.e. Polystyrene (PS), Polypropylene (PP), Low-density Polyethylene (LDPE), and Nylon, in a simulated in vitro human digestive system. Firstly, the changes in the physicochemical properties of 20–210 μm sized MPs as well as the leaching of chemicals were monitored using fluorescence microscopy, FTIR, and LC-QTOF-MS. Thereafter, the mass loss and morphological alterations in 3–4 mm sized MPs were observed after removing the organic matter. The interaction of PS and PP MPs with duodenal and bile juices manifested in a corona formation. The increase in surface roughness in PP MPs aligned with MP-enzyme dehydrogenation reactions and the addition of NO groups. A few fragments ranging from 30 to 250 μm, with negligible mass loss, were released during the MP digestion process. In addition, the leaching of compounds, e.g. capsi-amide, butanamide, and other plasticizers and monomers was also observed from MPs during digestion, and which may have the potential to accumulate and get absorbed by the digestive organs, and to subsequently impart toxic effects.

Retention of ZnO nanoparticles onto polypropylene and polystyrene microplastics: Aging-associated interactions and the role of aqueous chemistry

Microplastics (MPs) are pervasive and undergo environmental aging processes, which alters potential interaction with the co-contaminants. Hence, to assess their contaminant-carrying capacity, mimicking the weathering characteristics of secondary MPs is crucial. To this end, the present study investigated the interaction of Zinc oxide (nZnO) nanoparticles with non-irradiated (NI) and UV-irradiated (UI) forms of the most abundant MPs, such as polypropylene (PP) and polystyrene (PS), in aqueous environments. SEM images revealed mechanical abrasions on the surfaces of NI-MPs and their subsequent photoaging caused the formation of close-ended and open-ended cracks in UI-PP and UI-PS, respectively. Batch-sorption experiments elucidated nZnO uptake kinetics by PP and PS MPs, suggesting a sorption-desorption pathway due to weaker and stronger sorption sites until equilibrium was achieved. UI-PP showed higher nZnO (∼3000 mg/kg) uptake compared to NI-PP, while UI-PS showed similar or slightly decreased nZnO (∼2000 mg/kg) uptake compared to NI-PS. FTIR spectra and zeta potential measurements revealed electrostatic interaction as the dominant interaction mechanism. Higher nZnO uptake by MPs was noted between pH 6.5 and 8.5, whereas it decreased beyond this range. Despite DOM, MPs always retained ∼874 mg/kg nZnO irrespective of MPs type and extent of aging. The experimental results in river water showed higher nZnO uptake on MPs compared to DI water, attributed to mutual effect of ionic competition, DOM, and MP hydrophobicity. In the case of humic acids, complex synthetic and natural water matrices, NI-MPs retained more nZnO than UI-MPs, suggesting that photoaged MPs sorb less nZnO under environmental conditions than non-photoaged MPs. These findings enhance our understanding on interaction of the MPs with co-contaminants in natural environments.

Size-selective attachment of polyvinyl chloride microplastics on iron oxides in aqueous environments

The interaction of microplastics (MPs) with mineral particles is a crucial process determining the fate of MPs in aquatic environments, but potential size-selective interaction of MPs with minerals is unknown. In this work, strong and multi-layered attachment of a mix of heterogeneously size of polyvinyl chloride (PVC) MPs was observed on all the four types of iron oxides, following an order of Fe3O4-i > Fe3O4-s > γ-Fe2O3 > Fe3O4-n. Electrostatic attraction dominated their strong attachment, while hydrogen bonding and halogen bonding also contributed to MPs-mineral interaction. Importantly, both attachment rates and capacities were more favorable for large-sized PVC MPs (> 1.0 μm) than small-sized MPs (0.6–1.0 μm), due to more available attachment sites and stronger electrostatic attraction. Large-sized MPs preferred to detach from iron oxides before small-sized MPs, confirming by configuration analysis. The attachment accelerated the sedimentation of PVC MPs, while a fraction (i.e., 26.7 %, PVC/iron oxide ratio at 0.2:1) of heteroaggregates floated at the air–water interface associated with bubbles. Natural organic matter decreased MPs-mineral interaction due to competitive adsorption, electrostatic repulsion, and steric hindrance, while the attachment was enhanced with increasing ionic strengths (0–200 mM). These findings contribute to a better understanding of the transport and fate of MPs in aquatic systems.

Interaction between Microplastics and Pathogens in Subsurface System: What We Know So Far

Microplastics (MPs) are abundant in soil and the subsurface environment. They can co-transport with pathogens or act as vectors for pathogens, potentially causing severe ecological harm. The interaction of MPs with pathogens is an important topic. To describe the origins and features of MPs in the subsurface environment, we evaluated relevant studies conducted in the laboratory and field groundwater habitats. We explore the interactions between pathogens and microplastics from three perspectives including the respective physicochemical properties of microplastics and pathogens, external environmental factors, and the binding between microplastics and pathogens. The effects of some interaction mechanisms and environmental factors on their co-transport are discussed. The key factors affecting their interaction are the particle size, specific surface area, shape and functional groups of MPs, the zeta potential and auxiliary metabolic genes of pathogens, and the hydrophobicity of both. Environmental factors indirectly affect MPs and the interaction and co-transport process of pathogens by changing their surface properties. These findings advance our knowledge of the ecological behavior of MPs–pathogens and the associated potential health hazards.

Microplastics enhance the adsorption capacity of zinc oxide nanoparticles: Interactive mechanisms and influence factors

Microplastics (MPs) are of particular concern due to their ubiquitous occurrence and propensity to interact and concentrate various waterborne contaminants from aqueous surroundings. Studies on the interaction and joint toxicity of MPs on engineered nanoparticles (ENPs) are exhaustive, but limited research on the effect of MPs on the properties of ENPs in multi-solute systems. Here, the effect of MPs on adsorption ability of ENPs to antibiotics was investigated for the first time. The results demonstrated that MPs enhanced the adsorption affinity of ENPs to antibiotics and MPs before and after aging showed different effects on ENPs. Aged polyamide prevented aggregation of ZnONPs by introducing negative charges, whereas virgin polyamide affected ZnONPs with the help of electrostatic attraction. FT-IR and XPS analyses were used to probe the physicochemical interactions between ENPs and MPs. The results showed no chemical interaction and electrostatic interaction was the dominant force between them. Furthermore, the adsorption rate of antibiotics positively correlated with pH and humic acid but exhibited a negative correlation with ionic strength. Our study highlights that ENPs are highly capable of accumulating and transporting antibiotics in the presence of MPs, which could result in a widespread distribution of antibiotics and an expansion of their environmental risks and toxic effects on biota. It also improves our understanding of the mutual interaction of various co-existing contaminants in aqueous environments.

The synergy of microplastics with the heavy metal zinc has resulted in reducing the toxic effects of zinc on lentil (Lens culinaris) seed germination and seedling growth

There is growing recognition of the impact of the rising presence of microplastics (MPs) on terrestrial plant growth and, in general, the terrestrial ecosystem. Simultaneously, there is growing heavy metal accumulation in agricultural lands at an astonishing rate owing to the overwhelming use of chemical fertilizers, herbicides, and weedicides. Thus, there is a need to investigate the synergetic effect of MPs along with heavy metals on the inducing combined toxicity. This study investigates effects at smaller exposure periods of a few hours using a novel optical imaging technique, Biospeckle Coherence Tomography. Biospeckle Optical Coherence Tomography (bOCT) is a novel optical imaging technique that we successfully demonstrated earlier in visualizing the internal activity of plants. Previous studies of authors using the bOCT technique have demonstrated its potential in the independent application of polyethylene microplastic (PEMPs) as well as zinc within 6 h after their treatments. The strong inhibitory effect of 100 mg L−1, Zn, and PEMPs alone on the germination of Lens culinaris could be visualized with bOCT. The current study demonstrated that against expectation, combined effects of Zn toxicity were reduced when combined with MPs. This is suggested due to the significant reduction of Zn uptake by the seedlings through the interaction of Zn and MPs in an aqueous solution. Mass-spectrometry results also indicate a reduced intake of Zn. Our findings suggest that PEMPs could be able to reduce the over-availability of Zn, thus mitigating the Zn toxicity on lentils.

Effect of microplastics on the adherence of coexisting background organic contaminants to natural organic matter in water

Microplastics (MPs) may interact with background organic substances (including natural organic matter and organic pollutants) after entering the aquatic environment and affect their original binding. Thus, the interaction of MPs with background organic substances (i.e., humic acid (HA), polychlorinated biphenyls (PCBs), and hydroxy PCBs) were elucidated. According to the results, PCB and hydroxy PCB displayed a strong propensity to adhere to HAs in the absence of MPs. However, the PCBs and hydroxy PCBs that were initially bound to HAs shifted from HAs to MPs in the presence of MPs. Further analysis demonstrated that this transfer was dominated by van der Waals interactions, with hydrogen bond interactions as an additional driving force. Upon the interaction, large MPs-HA-PCB/ hydroxy PCB aggregates with MPs as the core and HAs as the outermost layer were formed. Significant changes in the properties of background organic matter, including the distribution of PCB/hydroxy PCB around HA, diffusion coefficient, and hydrogen bond networks in the HA-PCB/ hydroxy PCB domains, occurred during the MP–HA–PCB/hydroxy PCB interaction. These results provide molecular-level evidence that the intrusion of MPs changes the binding preference of background organic pollutants and can lead to a redistribution of background organic pollutants.

Global hotspots and trends in interactions of microplastics and heavy metals: a bibliometric analysis and literature review.

Microplastics (MPs) are identified as emerging contaminants; however, their interactions with heavy metals in the environment have not been well elucidated. Here, the research progress, hotspots, and trends in the interactions of MPs and heavy metals were analyzed at a global scale using a bibliometric analysis combined with a literature review. We comprehensively searched the Web of Science Core Collection database from 2008 to July 5, 2022. A total of 552 articles published in 124 journals were selected, which came from 70 countries and 841 institutions. The most contributing journals, countries, institutions, and authors were identified. Visualization methods were used to identify high co-citation references and hot keywords in the 552 articles. Evolutionary and cluster analyses of hot keywords suggested several research hotspots in the co-contamination of MPs and heavy metals, including their toxicity and bioaccumulation, the adsorption and desorption behaviors, the environmental pollution and risk assessment, and their detection and characterization. Based on the current research status, several directions of priority are recommended to understand the interactions between MPs and heavy metals and their potential risks. This article can help recognize the current research status and future directions in this field.

Small polystyrene microplastics interfere with the breakdown of milk proteins during static in vitro simulated human gastric digestion

Human ingestion of microplastics (MPs) is common and inevitable due to the widespread contamination of food items, but implications on the gastric digestion of food proteins are still unknown. In this study, the interactions between pepsin and polystyrene (PS) MPs were evaluated by investigating enzyme activity and conformation in a simulated human gastric environment in the presence or absence of PS MPs. The impact on food digestion was also assessed by monitoring the kinetics of protein hydrolysis through static in vitro gastric digestion of cow's milk contaminated with PS. The binding of pepsin to PS showed that the surface chemistry of MPs dictates binding affinity. The key contributor to pepsin adsorption seems to be π−π interactions between the aromatic residues and the PS phenyl rings. During quick exposure (10 min) of pepsin to increasing concentrations (222, 2219, 22188 particles/mL) of 10 μm PS (PS10) and 100 μm PS (PS100), total enzymatic activities were not affected remarkably. However, upon prolonged exposure at 1 and 2 h, preferential binding of pepsin to the small, low zeta-potential PS caused structural changes in the protein which led to a significant reduction of its activity. Digestion of cow's milk mixed with PS10 resulted in transient accumulation of larger peptides (10–35 kDa) and reduced bioavailability of short peptides (2–9 kDa) in the gastric phase. This, however, was only observed at extremely high PS10 concentration (0.3 mg/mL or 5.46E+05 particles/mL). The digestion of milk peptides, bound preferentially over pepsin within the hard corona on the PS10 surface, was delayed up to 15 min in comparison to bulk protein digestion. Intact caseins, otherwise rapidly digested, remained bound to PS10 in the hard corona for up to 15 min. This work presents valuable insights regarding the interaction of MPs, food proteins, and pepsin, and their dynamics during gastric digestion.

Interfacial Interactions of Uranium and Arsenic with Microplastics: From Field Detection to Controlled Laboratory Tests.

We studied the co-occurrence of microplastics (MPs) and metals in field sites and further investigated their interfacial interaction in controlled laboratory conditions. First, we detected MPs in freshwater co-occurring with metals in rural and urban areas in New Mexico. Automated particle counting and fluorescence microscopy indicated that particles in field samples ranged from 7 to 149 particles/L. The urban location contained the highest count of confirmed MPs, including polyester, cellophane, and rayon, as indicated by Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy analyses. Metal analyses using inductively coupled plasma (ICP) revealed that bodies of water in a rural site affected by mining legacy contained up to 332.8 μg/L of U, while all bodies of water contained As concentrations below 11.4 μg/L. These field findings motivated experiments in laboratory conditions, reacting MPs with 0.02-0.2 mM of As or U solutions at acidic and neutral pH with poly(methyl-methacrylate), polyethylene, and polystyrene MPs. In these experiments, As did not interact with any of the MPs tested at pH 3 and pH 7, nor U with any MPs at pH 3. Experiments supplied with U and MPs at pH 7 indicated that MPs served as substrate surface for the adsorption and nucleation of U precipitates. Chemical speciation modeling and microscopy analyses (i.e., Transmission Electron Microscopy [TEM]) suggest that U precipitates resemble sodium-compreignacite and schoepite. These findings have relevant implications to further understanding the occurrence and interfacial interaction of MPs and metals in freshwater.

Combined effects of microplastics and cadmium on the soil-plant system: Phytotoxicity, Cd accumulation and microbial activity

Microplastics (MPs), an emerging pollutant of concern, widely cooccurred with heavy metals in soil, however, little is known about the combined effects of the interactions of MPs and cadmium (Cd) on the soil-plant system. In this study, the combined effects of several types of MPs and soil Cd contamination on Brassica juncea growth, Cd uptake, and soil microbial carbon metabolism were investigated in a 50-day pot experiment. Aged polyethylene (PE), aged polypropylene (PP), biodegradable polybutylene adipate terephthalate (PBAT) and polylactic acid (PLA) displayed moderate phytotoxicity, with reductions in leaf chlorophyll content and shoot biomass. Compared with the control treatment without MPs or B. juncea, B. juncea growth significantly increased the soil pH by 0.3 pH units, and the growth of B. juncea in the presence of biodegradable PBAT or PLA MPs increased the soil pH by an additional 0.4 or 0.6 pH units, respectively. The presence of PBAT or PLA MPs greatly reduced soil diethylenetriamine pentaacetic acid (DTPA)-extractable Cd concentrations and plant Cd accumulation. The Cd bioconcentration factor was higher in roots than shoots in all treatments except the treatment containing PBAT MPs. The average well color development (AWCD), an indicator of metabolic activity, was highest in the treatment with B. juncea alone and was reduced by both biodegradable and conventional MPs. The microbial utilization efficiency of esters and alcohols was enhanced in the treatment with PBAT MPs, whereas carboxylic acids were preferentially utilized in the treatment with PLA MPs. These findings indicate that co-exposure to MPs and Cd may alter soil microenvironmental characteristics such as soil pH, leading to changes in Cd bioavailability, plant growth and Cd accumulation, and the microbial community's capacity to metabolize carbon. These effects of MPs in soil warrant further exploration.