Interaction Of L-cysteine Research Articles

Electrochemical and Spectroscopic Investigations of L-Glutathione with Bismuth Pharmaceuticals

Introduction The electrochemical and spectroscopic investigation of bismuth(III) compounds with L-cysteine has been carried out in this laboratory since our initial publication.1 Cyclic voltammetry has shown that the interaction of L-cysteine with bismuth nitrate, bismuth salicylate, and bismuth citrate increases the solubility of these compounds by complexation with L-cysteine, thereby increasing the reduction current for the bismuth complexes formed. The appearance of a UV-VIS spectroscopic band at 340 nm indicates the formation of a Bi-S bond, the sulfur being supplied by the L-cysteine. These investigations have been conducted at pH 1.00 and 3.00 to simulate conditions in the human stomach and at pH 7.40 MOPS [(3-N-morpholino)propanesulfonic acid] to simulate general physiological conditions, in particular the intestinal tract. In this presentation, continuing studies of L-glutathione, a tripeptide incorporating L-cysteine, under these conditions shall be discussed. This work is applicable to the important field of bismuth pharmaceuticals as a means of understanding the interaction of these compounds under various conditions in the human digestion system.2 Results and Discussion Initial studies of L-glutathione with bismuth nitrate, bismuth salicylate, and bismuth citrate have been carried out under conditions similar to those of our initial work.1 It has been found that the general characteristics of the cyclic voltammograms for L-glutathione (GSH) are similar to those for L-cysteine; however, the slower rate of complexation of GSH with these bismuth compounds is evident in the changes in voltammetric features in successive sweeps. In the case of GSH addition to bismuth citrate in pH 7.4 MOPS buffer, for instance, the first sweep shows reduction of the bismuth:GSH complex at -0.71 V vs Ag/AgCl at a gold electrode, whereas a reduction process at -0.60 V is observed on the second sweep. Similar results are obtained at glassy carbon. The bismuth:GHS complex is destroyed during the reduction process as a bismuth film forms on the electrode surface. The following sweep, then, is indicative of the complexation environment in solution at that point. The bismuth ion, restored in the stripping step, apparently does not have time in the few seconds after the stripping process to fully form a complex with GSH. The observed reduction potential around -0.60 V is due to the reduction of another bismuth complex, perhaps with citrate. Further investigations are in progress to more fully describe this situation.References G. T. Cheek and D. Peña, J. Electrochem. Soc., 167, 155522 (2020)H. Li, R. Wang, and H. Sun, Acc. Chem. Res., 52, 216 (2019).

(Digital Presentation) Electrochemical and UV-VIS Spectroscopic Studies of Bismuth(III) Interactions with L-Cysteine

IntroductionIn an ongoing investigation of the interaction of bismuth(III) compounds with L-cysteiene1, recent work has involved more complete characterization of these interactions at lower pH levels in various aqueous media. Pharmaceutical medications2 such as bismuth subsalicylate (BSS) and bismuth citrate (BC) have now been investigated at pH 1.00 and 3.00 by both cyclic voltammetric and UV-VIS methods. Electrochemical methods have been used to evaluate the interaction of L-cysteine with bismuth compounds by observing shifts in the potential for bismuth reduction, and UV-VIS methods have allowed the observation of an absorbance peak at approximately 340 nm due to the bismuth(III) ion interaction with the L-cysteine thiol group.Results and DiscussionRecent work has involved investigation of bismuth(III) nitrate (BN), BSS, and BC with L-cysteine at pH 1.00 and 3.00 in both HNO3 and HCl solutions. In general, complexation of BN is not quite as favorable at lower pH levels as in pH 7.00 MOPS (3-N-morpholino)propanesulfonic acid)3 buffer solution.1 This conclusion was based on the need for higher mole ratios of L-cysteine to BN to achieve the final state of the voltammograms. The UV-VIS behavior was also not as well-defined as in our previous work1, with higher molar ratios of L-cysteine needed to produce the 340 nm absorbance peak. These results support the competition of protons with bismuth(III) for the L-cysteine ligand, there being much higher proton levels at pH 1.00 and pH 3.00 than at pH 7.00. The behaviors of BC and BSS at pH 1.00 and pH 3.00 are in many ways similar to that of BN. Finally, some preliminary work on glutathione (cysteine-containing tripeptide) interactions with bismuth compounds is also planned for presentation.References T. Cheek and D. Peña, J. Electrochem. Soc., 167, 155522 (2020)Li, R. Wang, and H. Sun, Acc. Chem. Res., 52, 216 (2019).M. H. Ferreira, I. S. S. Pinto, E. V. Soares, and H. M. V. M. Soares, RSC Advances, 5(39), 30989 (2015).

Electrochemical and Spectroscopic Investigations of Bismuth Pharmaceuticals

IntroductionThe use of bismuth compounds as pharmaceutical agents for gastric maladies has a long history.1 Many techniques have been used to characterize the mode of operation of these compounds; however, electrochemical methods have not been fully utilized in this regard. The sulfhydryl side chain of the amino acid L-cysteine and related compounds can form complexes with bismuth(III) ions,1 so that the effect of L-cysteine added to solutions of bismuth(III) compounds is expected to greatly affect the electrochemical behavior of this system. Previous work in this laboratory has involved electrochemical investigation of both L-cysteine in various media2, and the interaction of bismuth(III) compounds with L-cysteine.3 The present work involves the interaction of L-cysteine with bismuth-containing pharmaceuticals.Results and DiscussionBismuth(III) nitrate has been chosen for the initial studies, followed by work with bismuth(III) citrate and bismuth(III) salicylate, both of which are used in commonly available pharmaceuticals. Our studies have been carried out in 0.100 M HCl and 0.100 M HNO3 in order to simulate the pH level of gastric contents. The electrochemical techniques cyclic voltammetry and square wave voltammetry were used in this work, and some comparisons between the results are intended for presentation. Voltammetric scans have been carried out at both glassy carbon and boron-doped diamond electrodes in order to avoid the reduction of protons, which would occur at platinum and gold electrodes. Although the solubility of Bi(NO3)3 in 0.10 M HNO3 is rather low, initial studies revealed that the addition of L-cysteine to the solution greatly increased the solubility of bismuth(III) nitrate, as seen by an increase in the bismuth(III) reduction current as well as the subsequent stripping current of the bismuth deposit.Bismuth(III) salicylate was found to be soluble at the 1 mM level in 0.100 HNO3, and L-cysteine additions produced potential shifts in the voltammetric processes for the bismuth(III) system. These results support the complexation of bismuth(III) salicylate by L-cysteine, even under these very acidic conditions. This work has now been extended to bismuth(III) citrate in 0.10 M HCl, with overall similar results. Studies in pH 7.4 MOPS buffer have also been carried out to simulate pH levels in other parts of the human body. The electrochemical behavior of bismuth(III) nitrate in pH 7.4 MOPS (3-(N-morpholino)propanesulfonic acid)4 buffer was investigated in this regard. Whereas bismuth(III) nitrate (nominal 1.0 mM) was essentially insoluble in this medium, additions of L-cysteine produced voltammetric currents typical of the 1 mM concentration level. As at pH 1.00, initial reduction of bismuth(III) was followed by bismuth stripping peaks at more positive potentials. This behavior supports the complexation of bismuth(III) ion by L-cysteine. Further evidence of this complexation was found by UV-VIS studies of bismuth(III) nitrate in pH 7.4 MOPS buffer with incremental additions of L-cysteine. An absorbance band at 325 nm appeared as L-cysteine was added, the absorbance of which remained constant after the 1:2 Bi3+: L-cysteine point. These results indicate formation of Bi(Cys)2 under these conditions. As a whole, the present studies indicate the ability of bismuth(III) compounds to interact with L-cysteine at pH 1.0 and pH 7.4.References Li, R. Wang, and H. Sun, Acc. Chem. Res., 52, 216 (2019).T. Cheek and M. A. Worosz, ECS Trans., 164(12), D707 (2016).T. Cheek and D. Pena, J. Electrochem. Soc., 167, 155522 (2020).M. H. Ferreira, I. S. S. Pinto, E. V. Soares, and H. M. V. M Soares, RSC Advances, 5(39), 30989 (2015).

Electrochemical Investigations of L-Cysteine Interactions with Bismuth Ions

The interaction of L-cysteine with bismuth compounds bismuth(III) salicylate, bismuth(III) citrate, and bismuth(III) nitrate, was studied at pH 1.0 (0.100 M HNO3 and 0.100 M HCl) and pH 7.4 MOPS buffer by cyclic voltammetry at glassy carbon and boron-doped diamond electrodes. pH 1.0, at which bismuth (III) exists as the simple Bi3+ ion, was chosen to approximate the acid strength of stomach contents. pH 7.4, at which bismuth(III) exists as BiO, was used for its similarity to general physiological conditions. The amino acid L-cysteine was chosen because its sulfhydryl group undergoes intense interaction with many metal cations, serving as a model for cysteine-containing proteins in the digestive system. It was determined that Bi(III) and L-cysteine (Cys) form soluble complexes at both pH 1.0 and pH 7.4. UV–vis spectroscopic investigations support interaction of Bi(III) and L-cysteine to form a 1:2 Bi(III): Cys complex in pH 7.4 MOPS buffer. L-cysteine addition to solutions of the pharmaceutical bismuth(III) salicylate was found to alter the voltammetric behavior of the salicylate complex. These results, especially at pH 1.0, are relevant to understanding the interaction of various cysteine-containing proteins in the human digestive system with bismuth pharmaceuticals and may help guide future explorations of bismuth formulations.

Electrochemical Investigations of L-Cysteine Interactions with Bismuth Ions

The interaction of L-cysteine with various bismuth compounds, namely, bismuth(III) salicylate, bismuth(III) citrate, and bismuth(III) nitrate, was studied at pH 1.0 (0.100 M HNO3 and 0.100 M HCl) and pH 7.4 MOPS buffer by cyclic voltammetry at glassy carbon and boron-doped diamond electrodes. Using details of the voltammetric behavior, it was determined that Bi3+ and L-cysteine (Cys) form a soluble complex at both pH 1.0 and pH 7.4. In addition, the reduction of the bismuth-cysteine complex to bismuth metal results in subsequent bismuth stripping. UV-VIS spectroscopy revealed that Bi3+ and L-Cysteine form a 1:2 Bi3+: Cys complex in pH 7.4 MOPS buffer.

A sensitive electrochemical sensor for Pb2+ ions based on ZnO nanofibers functionalized by L-cysteine

Environmental pollution caused by heavy metals is a world concern, mainly because it can be accumulated in the human body, causing serious health problems. Thus, an accurate method to determine the concentration of heavy metals, lead (II) ions for instance, for water quality control is of great significance to the protection of the environment and human health. Herein, we reported the synthesis of a zinc oxide nanofibers/L-cysteine (ZnO_L-cys) nanocomposite and its use as electrodes for the electrochemical sensing of Pb2+ ions. First, ZnO nanofibers were prepared by electrospinning method followed by annealing. Then, L-cysteine was anchored into the surface of ZnO nanofibers for producing the work electrode. Square wave anodic stripping voltammetry (SWASV) was applied for the detection of Pb2+. The ZnO_L-cys modified glass carbon electrode (GCE/ZnO_L-cys) showed considerably higher response in relation to the non-functionalized ZnO nanofibers. Several optimization parameters were studied and under optimized conditions the GCE/ZnO_L-cys electrode displayed excellent sensitivity, selectivity, and stability. The limit of detection (LOD) was 0.397 μg·L−1 in the linear range of 10–140 μg·L−1. Also, the sensor was successfully applied for the Pb2+ ion in real samples, with recoveries of 94% and 95%. The success of electrochemical performance is a result of the high porosity of nanofibers combined with Lewis acid-base interaction of L-cysteine, making it great material for Pb2+ detection in real sample.

Calorimetric and Spectroscopic Studies to Monitor the Interaction of L-Cysteine Functionalized Selenium Nanoparticles with Calf Thymus DNA

The focus of the study is to understand the interaction between calf thymus DNA and L-Cysteine capped Selenium nanoparticles (cys-SeNPs) using spectroscopic and calorimetric techniques. The size of the nanoparticles was confirmed using transmission electron microscope (TEM). Isothermal titration calorimerty (ITC) provided the binding energy, the number of binding sites and the thermodynamic parameters. UV-Vis absorption and fluorescence quenching studies established the number of binding sites and binding constant. Stabilization of the ct-DNA in the presence of cys-SeNPs was determined from thermal melting studies. It is confirmed by the spectrophotometric titration and calorimetric studies that, selenium nanoparticles can form a complex with the double–helical DNA. The binding constant of cys-SeNPs with the DNA from fluorescence study was found to be 2.953 × 106 M−1 and is consistent with the value obtained from ITC experiment. The binding energies along with the binding sites symbolize the crucial role of van der Waals forces and hydrogen bonds in the binding of cys-SeNPs with ct-DNA.

One-Step Synthesis of CuO–Cu2O Heterojunction by Flame Spray Pyrolysis for Cathodic Photoelectrochemical Sensing of l-Cysteine

CuO-Cu2O heterojunction was synthesized via a one-step flame spray pyrolysis (FSP) process and employed as photoactive material in construction of a photoelectrochemical (PEC) sensing device. The surface analysis showed that CuO-Cu2O nanocomposites in the size less than 10 nm were formed and uniformly distributed on the electrode surface. Under visible light irradiation, the CuO-Cu2O-coated electrode exhibited admirable cathodic photocurrent response, owing to the favorable property of the CuO-Cu2O heterojunction such as strong absorption in the visible region and effective separation of photogenerated electron-hole pairs. On the basis of the interaction of l-cysteine (l-Cys) with Cu-containing compounds via the formation of Cu-S bond, the CuO-Cu2O was proposed as a PEC sensor for l-Cys detection. A declined photocurrent response of CuO-Cu2O to addition of l-Cys was observed. Influence factors including CuO-Cu2O concentration, coating amount of CuO-Cu2O, and applied bias potential on the PEC response toward l-Cys were optimized. Under optimum conditions, the photocurrent of the proposed sensor was linearly declined with increasing the concentration of l-Cys from 0.2 to 10 μM, with a detection limit (3S/N) of 0.05 μM. Moreover, this PEC sensor displayed high selectivity, reproducibility, and stability. The potential applicability of the proposed PEC sensor was assessed in human urine samples.

Interaction of l-cysteine functionalized CdSe quantum dots with metallic cations and selective binding of cobalt in water probed by fluorescence

Water-soluble CdSe quantum dots (QDs) capped with l-cysteine (Cys-CdSe) were synthesized in aqueous medium and analyzed by X-ray diffraction, electronic microscopy, absorption spectroscopy and time-resolved fluorescence spectroscopy. We have measured the average diameter of Cys-CdSe QDs, 4.15nm, the true molecular mass, 1.43×105gmol−1 and the molar extinction coefficient, ε480=3×105cm−1M−1 at maximum of band edge (480nm). The number of grafted l-cysteine chains per individual QD was measured to be ∼100. The interaction of these functionalized Cys-CdSe QDs at a concentration of 0.2μM with seventeen different metal ions were evaluated by fluorescence. Only the interaction with Co2+ ions resulted in fluorescence quenching in the range 0.5–20μM when the true concentration of QDs is 0.2μM, with a saturation behavior at Co2+ concentration of ∼20μM, in agreement with 100 grafted l-cysteines per QD. The quenching mechanism involves both static and dynamic fluorescence quenching processes. A model of interaction is derived for the selective binding of Co2+ to Cys-CdSe QDs, involving the carboxyl functions of l-cysteine. The comparison with other QD-systems shows the need for a systematic analysis of the parameters influencing the QD-ions interaction and fluorescence emission, especially their true concentration, in order to understand the fundamental mechanisms at the origin of the specificity for metal binding to a particular QD.

The light modulation of the interaction of l-cysteine with porphyrins coated ZnO nanorods

The photoenhancement properties of metalloporphyrins coated ZnO nanostructures have been demonstrated for both gas and liquid sensing. In gas sensing the light favours the electron transfer from the adsorbed analyte and the porphyrin, while in solution the increased catalytic effects of metalloporphyrins should also be considered.In this paper we report the sensing properties of copper and manganese porphyrins coated ZnO nanorods with respect to a thiolated biomolecule such as the l-cysteine. A one-pot hydrothermal growth method has been used to prepare the functionalized nanostructures onto an ITO substrate. The ensemble ITO–ZnO–porphyrin has been used as the working electrode in a three-electrodes amperometric setup. I/V curves have been measured at different concentrations of l-cysteine and interfering compounds. The I/V curves have been analyzed by principal component analysis, and the first principal component has been used as the sensor feature.The results show that illumination enhances the sensitivity to l-cysteine and the selectivity towards a set of non-thiolated aminoacids and organic acids.

Solvent-Mediated Eco-Friendly Synthesis and Characterization of Monodispersed Bimetallic Ag/Pd Nanocomposites for Sensing and Raman Scattering Applications

The solvent-mediated eco-friendly monodispersed Ag/Pd bimetallic nanocomposites (BNCs) having thick core and thin shell have been prepared through novel green chemical solvent reduction method. Reducing solvent, dimethyl formamide (DMF) is employed for the controlled green synthesis. Characterization of the synthesized Ag/Pd BNCs has been done by x-ray diffraction (XRD) studies, high-resolution scanning electron microscopy (HR-SEM), energy-dispersive X-ray analysis (EDX), and high-resolution transmission electron microscopy (HR-TEM) with selected area electron diffraction (SAED) pattern. The nature of the interaction of L-cysteine with Ag/Pd BNCs has been studied by using surface plasmon spectroscopy, Fourier transform-infrared spectroscopy (FT-IR), cyclic voltammetry (CV), and theoretical methods.

Electrochemical studies on the interaction of l-cysteine with metallic copper in sulfuric acid

Amino acids have been widely used as green corrosion inhibitors for an array of metals. Considering its importance in corrosion chemistry, studies were undertaken with the objective to discovering the inhibitory effect of a sulfur-containing amino acid, l-cysteine, on copper in different concentrations of sulfuric acid (0.5, 1.0, and 1.5 M) at different temperatures. Techniques like the weight loss method, electrochemical impedance spectroscopy, potentiodynamic polarization (Tafel), and adsorption studies were employed. Results revealed that l-cysteine do offer an attractive inhibition efficiency. However, with an increase in the concentration of the inhibitor, corrosion rates decreased irrespective of the temperature gradients. This is due to surface adsorption of the inhibitor molecules on the metal which has contributed to a decreased double-layer capacitance and increased polarization resistance. With the increase in the concentration of the medium, the corrosion rate was also enhanced and this is due the liberation of a high quantum of H+ ions. Based on the results of Tafel polarization studies, it is evident that the amino acid, l-cysteine, could act as a mixed type inhibitor. The importance of l-cysteine in the corrosion of copper metal has been highlighted in this paper.

Photoelectron spectroscopic study on the interaction of l-cysteine with the silver-based dental alloy

The electronic structure of the silver-based dental alloy MC12 and its constituent metals and their interaction with l-cysteine have been investigated by ultraviolet photoelectron spectroscopy (UPS) using synchrotron radiation. The UPS spectra of l-cysteine on polycrystalline Ag, Cu, Pd, and Au substrates have been measured to understand the interaction of l-cysteine with the dental alloy surface. It was found that the electronic states of MC12 originate dominantly from Cu 3d states and Pd 4d states around the top of the valence bands, while the 4–7-eV electronic structure of MC12 originates from the Ag 4 d5/2 and Ag 4 d3/2 states. For l-cysteine, it was found that a new peak at 2 eV is observed for the thin films on Ag, Cu, and Au, while the structure around 2 eV on MC12 is similar to that on Pd. The shift of the 5-eV peak is observed for the thin films on Cu, Pd, and Au, but not on Ag and MC12. These results indicate that the interaction of l-cysteine with MC12 may be dominantly due to the Pd-S, Cu-S, and Ag-O bonds, while the contribution of the Ag-S bond is small.

Inhibiting the color formation by gradient temperature‐elevating Maillard reaction of soybean peptide‐xylose system based on interaction of l‐cysteine and Amadori compounds

Light color and savory flavor enhancer are attractive for consumers and food producers. The effect of addition time of l-cysteine on inhibiting color formation was investigated in soybean peptide-xylose system, and the possible pathway was explored. Once dicarbonyl compounds were formed during the Maillard reaction, the addition of l-cysteine had no color-inhibiting effect; if l-cysteine was added immediately after the Amadori compound was formed, the extraordinary color-inhibiting effect was observed. Therefore, an improved way to inhibit color formation was proposed on the basis of the interaction of l-cysteine and Amadori compounds by controlling the addition time of l-cysteine through gradient temperature-elevating Maillard reaction. The system was heated at 80 °C for 60 min to form Amadori compounds, followed by the addition of L-cysteine, and the temperature was raised to 120 °C and held for 110 min. Compared with traditional products, the lightest color product was found desirable by GC/MS analysis and sensory evaluation. The novel method proposed can be a guide for the industrial preparation of light-colored products.

Interaction of l-cysteine with naked gold nanoparticles supported on HOPG: a high resolution XPS investigation

We report the results of a synchrotron-based high-resolution XPS study of the interaction of L-cysteine (Cys) with well-characterized colloidal gold nanoparticles (NPs, typical size 3-4 nm), which were pre-deposited on highly oriented pyrolytic graphite and then brought into contact with the aqueous solution of Cys by drop-casting. By comparison with data previously obtained for Cys deposition on flat Au substrates (single crystals and high quality films), we demonstrate the formation of a strong Cys/NP thiolate bond. The analysis of the line shape and adsorbate-induced Au 4f core level shift, backed by simulations of the NP structure, reveals the interaction of Cys with low-coordinated Au atoms belonging to the NP edge and corners. The analysis of the N 1s core-level indicates that neutral molecules are the most abundant species. The small facet size limits the formation of extended networks of zwitterionic molecules, typical of single crystal surfaces. This study provides a spectroscopic insight into the intense poisoning effect caused by a limited amount of Cys on Au catalysts described in previous reports.

Thermochemical characteristics of the interaction of L-cysteine with glycerol, ethylene glycol, and 1,2-propylene glycol in aqueous solutions

Integral enthalpies of dissolution ΔsolHm of L-cysteine in mixtures of water with glycerol, ethylene glycol, and 1,2-propylene glycol at concentrations of organic solvents of up to 0.37 mole fraction were measured by calorimetry of dissolution. The standard values of the dissolution enthalpies (ΔsolH°) and the transfer enthalpies (ΔtrH°) of amino acid from water to the mixed solvent were calculated. It was shown that the calculated enthalpic coefficients of the pair interactions of L-cysteine with cosolvent molecules have positive values. The obtained data are interpreted from the viewpoint of the prevalence of different types of interactions in the solutions and influence of the cosolvents nature on the thermochemical characteristics of amino acid dissolution.

Solid-State NMR Study of Cysteine on Gold Nanoparticles

Solid-state NMR spectroscopy is used to characterize the interaction of l-cysteine with gold nanoparticles. The experiments show that there are two types of cysteine in the gold−cysteine complex, with nearly equal populations. We postulate that cysteine forms a two-layer boundary around the gold nanoparticles. The first layer is made of cysteine molecules forming a thiolate bond with the gold surface and having its charged amino and carboxyl groups oriented away from the gold surface. The second layer has its amino and carboxyl groups oriented toward the first layer and its sulfur group oriented away from the gold particles.

Interaction of L-cysteine with selenious and selenic acids: A study by the density functional theory method

Using the density functional theory method at the B3LYP/6-31G(d,p) theory level, the formation of hydrogen bonded complexes of L-cysteine with selenious and selenic acids is studied. In both cases, complexes formed through the carboxyl group of cysteine mostly arise, their enthalpy of formation being of -19kcal/mol to -21 kcal/mol and the free energy of -6kcal/mol to -9kcal/mol. The primary act of interaction in the system of hydroxyl-containing selenium compound — α-aminoacid, including the mutual orientation of reactant molecules and the formation of intermolecular hydrogen bonds is likely to a serve as prerequisite for the thiol group to be able to participate in the next stages (including deeper chemical transformations) of biologically significant reactions.

Kinetic and Mechanistic Study of the Interaction of L-cysteine and N-acetylcysteine with cis-[Pt(en)(H2O)2](ClO4)2 in Aqueous Medium

Kinetic and Mechanistic Study of the Interaction of L-cysteine and N-acetylcysteine with cis-[Pt(en)(H2O)2](ClO4)2 in Aqueous Medium

Cysteine functionalized copper organosol: synthesis, characterization and catalyticapplication

We herein report a facile one-pot synthesis, stabilization, redispersion and Cu–S interactionof L-cysteine and dodecanethiol (DDT) protected copper organosol in toluene fromprecursor copper stearate using sodium borohydride in toluene under a nitrogenatmosphere. Surface modification of the synthesized copper organosol with an amino acidL-cysteine and an alkanethiol (dodecanethiol, DDT) is accomplished by a thiolate bondbetween the used ligands and nanoparticle surface. The cysteine molecule binds thecopper surface via a thiolate and amine linkage but not through electrostaticinteraction with the carboxylate group due to the solvent polarity and dielectricmedium. Fourier transform infrared (FTIR) analysis was performed to confirm thesurface functionalization of the amino acid and DDT to the copper surface. Copperorganosol has been characterized by optical spectroscopy (UV/vis), transmissionelectron microscopy (TEM), x-ray photoelectron spectroscopy (XPS) and x-raydiffraction (XRD). The as-synthesized particles are spherical in shape and exhibit aMie scattering profile with an absorption maxima in the visible range. Coppernanoparticles capped by cysteine and/or DDT in non-aqueous media are foundto represent an interesting catalytic approach for the synthesis of octylphenylether.