Intermuscular Fat Research Articles

Carcass Characteristics and Meat Quality of Conventionally and Organically Reared Suckling Dairy Goat Kids of the Payoya Breed

Abstract The viability of conventional goat farms, such as the native Payoya dairy goat, could be improved by switching to organic production, but product quality needs to be ensured. The present work assesses the carcass and meat quality of Payoya kids raised under conventional and organic grazing-based systems. Twenty-four kids (12 males, 12 females) were selected from each system (n = 48). The slaughter live weight (8.52 vs. 8.28 kg), cold carcass weight (4.44 vs. 4.29 kg) and farm dressing percentage (51.7 vs. 50%) of the conventionally raised kids were significantly higher than those of the organic kids. The shoulder (first category) (21.7 vs. 22.3%) and long leg (32 vs. 32.9%) percentages were lower in the conventional than in the organic kids. The percentage contribution of the intermuscular fat (10.70 vs. 8.11%) to the shoulder weight was greater in the conventional kids, while the percentages of muscle (59.7 vs. 57.2%) and bone (24.7 vs. 22.8%) were higher in the organic kids. For the chemical composition, there were only differences between the two sexes in the percentage of fat (6.64 and 7.99% on dry matter, for male and female, respectively). For rheological variables, only differences were found in the water holding capacity (% water expelled), the meat of the organic females had a higher value (17%) than that of the conventional females (14%). For the meat colour, the conventional male kids returned the highest values for C* and Hº (14.32 and 64.34, respectively). Farms following conventional grazing-based management could easily switch to organic production. Most of the meat and carcass quality variables studied were very similar across the systems.

MRI – MUSCLE IMAGING: P.297Which muscle segmentation approach to use for obtaining the highest discriminate power in natural history neuromuscular diseases using quantitative NMRI?

The degree of fatty infiltration, as measured using water-fat NMR imaging separation techniques (such as Dixon) is now a widely accepted muscle imaging biomarker and an often proposed outcome measure in many neuromuscular disease longitudinal studies. The question remains which is the specific muscle (group) to evaluate during the study period. We aimed to compare different segmentation approaches (global vs individual muscles) in a variety of neuromuscular disorders: immune-mediated necrotizing myopathy (IMNM), inclusion body myositis (IBM), GNE myopathy (GNEM), Duchenne muscular dystrophy (DMD), limb-girdle muscular dystrophy types 2B and 2I, Pompe disease (GSD2) and spinal muscular atrophy (SMA). We used the standardized response mean (SRM) of the fat fraction (FF) change after 1 year to assess the sensitivity of the different approaches. In 109 patients (21 IMNM, 21 IBM, 10 GNEM, 20 DMD, 12 LGMD2B, 7 LGMD2I, 13 GSD2, 5 SMA) two NMR scans were obtained with one year between them; on a clinical 3T Siemens system. A fat-water separation 3-point Dixon NMR sequence (TE1/TE2/TE3= 2.75/3.95/5.15 ms; TR = 10 ms) was performed in 5 slices at the level of the thigh as well as at the level of the leg. Regions of interest were drawn in individual muscles, muscle groups and in the whole segment. Global segmentation gave a satisfactory SRM (≥0.8) in thigh for IMNM, IBM, GNEM, LGMD2B and DMD. In leg, this was the case for IMNM, GNEM, LGMD2B and DMD. In some diseases, higher SRMs were found in individual muscles or muscle groups (including for LGMD2I and SMA). We can conclude that global segmentation is in some cases at least as sensitive as individual muscle or muscle group segmentation, implying that individual muscle segmentation is not always mandatory. A significant increase in FF does not automatically imply a high SRM, which means that the best muscle candidates to detect a treatment effect are not systematically the ones experiencing the most and severe and fastest disease progression. Generalization of the concept is premature. Additionally, the extent of intermuscular fat needs to be further investigated as changes in the nutritional status will likely bias the evaluation of muscular FF. Also other NMR outcome measures might be interesting to look into in such as water T2 and muscle cross-sectional area.

Bioinformatics analysis and transcriptional regulation of TORC1 gene through transcription factors NRF1 and Smad3 in bovine preadipocytes

Intramuscular fat is the an important factor that defines meat quality; however, enhancing its deposition without increasing the other three adipose depots (subcutaneous, visceral, and intermuscular fat) is a challenge for animal science and the meat industry. The TORC1 is a key regulator of adipogenesis and its regulation in bovine intramuscular preadipocytes has not been studied. The TORC1 is a member of the gene family that codes for a binding proteins which regulate transcription of cAMP which, is a key regulator of adipogenesis. In the present study, expression and sub-cellular localization of the TORC1 gene was analyzed in bovine preadipocytes. Bioinformatics tools were applied to characterize TORC1. To investigate the molecular mechanism of bovine TORC1 gene regulation, we cloned a 1008 bp of the 5’UTR regulatory region into a luciferase reporter vector. Different fragments were amplified using 5’UTR unidirectional deletion of the TORC1 promoter. Site directed mutation, dual luciferase reporter assay, RNAi interference and DNA-protein interaction (EMSA) were used to validate the regulatory roles of Smad3 and NRF1 in the regulation of TORC1 gene in bovine preadipocytes. The core promoter region of the TORC1 gene was identified at a location −410 to −155 bp upstream of transcription start site. Different vectors were constructed through serial deletion of the 5’UTR flanking region and in combination with site directed mutations and transcription interference through siRNA or shRNA, two transcription factors of NRF1 and Smad3 were found to be repressors in the promoter of the TORC1 gene. These findings were further confirmed through Electrophoretic Mobility Shift Assay (EMSA) within nuclear extracts of bovine adipocytes. The core promoter region of the TORC1 gene, spanning from −410 to −155 bp upstream of the transcription start site was specified in this study and this information will provide opportunity for the improvement of intramuscular fat in cattle.

Growth Performance and Meat Characteristics of the First Awassi-Rambouillet Callipyge Backcross.

Simple SummaryThe Awassi sheep breed is very resilient in the Mediterranean region but shows poor meat quality. The callipyge mutation enhances growth and meat characteristics, and as such we bred Awassi-Rambouillet Callipyge carrier rams with Awassi ewes and compared meat quality in the first backcross of Awassi-Rambouillet callipyge carriers (CRAW), non carrier Rambouillet Awassi (RAW), and pure Awassi (AW) ram lambs in a fattening trial for 94 days. CRAW lambs showed mostly similar growth potential and meat characteristics to RAW lambs. However, CRAW lambs showed higher growth potential as compared to AW lambs, and better meat characteristics in the shoulders and legs, but not in tenderness. Fat tail weight did not change in CRAW as compared to AW lambs. Therefore, the introgression of the callipyge mutation in Awassi lambs allows for improvement in growth and meat, without impacting the currently desirable traits of the fat tail. This research allows the improvement of meat production and return on investment in the local flocks of Awassi sheep.The Awassi breed is desirable due to its resilient traits, but shows poor lean meat content on the carcass; the callipyge mutation may enhance growth and meat characteristics. The first backcross of callipyge Awassi–Rambouillet (CRAW) lambs was generated by mating Awassi ewes with heterozygous F1 (50 % Awassi and 50 % Rambouillet) rams for the callipyge mutation. A fattening trial with carriers of CLPG (CRAW), non-carriers (RAW), and Awassi (AW) ram lambs was conducted for 94 days and growth and meat characteristic parameters were recorded. Final body weight, average daily gain (ADG), feed conversion ratio, and dry matter intake, but not initial body weight, were greater in CRAW and RAW lambs as compared to AW lambs; hot and cold carcass, dressing percentage, and shoulder, rack, and loin weights were greater in CRAW vs. AW but not RAW lambs. Leg cuts were heavier in CRAW vs. both RAW and AW lambs; intermuscular and subcutaneous fat percentages were greater in CRAW as compared to AW but not RAW lambs. Non-carcass components showed kidney fat, kidney, liver and testes weights higher in CRAW lambs as compared to AW lambs, but these parameters were similar to those of RAW lambs. There were no differences in fat tail weight. Further, CRAW lambs had higher shear force and water holding capacity than RAW and AW lambs, while cooking loss was the lowest in CRAW lambs. In conclusion, the callipyge mutation with 25% Rambouillet genes can provide efficient improvements in growth and meat characteristics, with the exception of tenderness in Awassi sheep.

High Inter-muscular Fat Is Associated with Poor Mobility Function in Older Adults, Only When Accompanied by Low Muscle Area (P01-026-19)

ObjectivesTo determine whether the associations between thigh inter-muscular-fat area, anthropometric characteristics and physical performance varies in old adults with low vs. high thigh muscle cross-sectional area. MethodsCross-sectional analysis of year 6 data from 1903 participants (52.2% women) of the Health ABC study aged 74–85y. Mid-thigh muscle composition (by CT), body composition (DXA) and physical performance were compared across quartiles of mid-thigh inter-muscular fat area. General linear model univariate analysis was used to study the associations between inter-muscular fat area and physical function according to low or high mid-thigh muscle cross-sectional area (using the 50th percentile). ResultsIn participants with low mid-thigh muscle area, there was a threshold effect in which high inter-muscular fat was related to poor mobility function after the 2nd quartile (>23.7 cm2 in men and >21.3 cm2 in women). While, in participants with high mid-thigh muscle area, high inter-muscular fat was not associated with poor mobility function. Inter-muscular fat had no significant association with isokinetic knee strength in participants with either low or high muscle area. However, across all inter-muscular fat conditions, participants with low muscle area had lower leg strength than those with high muscle area. ConclusionsThe negative effects of inter-muscular fat in aging are greatly influenced by the presence of concurrent low muscle mass. Funding SourcesNIA/NIH.

Growth performance and meat characteristics of the first filial Awassi-Rambouillet callipyge ram lambs.

Aim:This study was designed to introduce the callipyge (CLPG) and 50% of Rambouillet sheep genes to improve meat quality and quantity of Awassi (AW) sheep.Materials and Methods:The CLPG mutation was introduced into the AW sheep through frozen semen of homozygous Rambouillet rams for the CLPG mutation. Four ram lambs from the first-generation Rambouillet callipyge Awassi (F1-RCA) and five from pure AW were recruited for a fattening trial conducted in individual pens using standard ration, following which ram lambs were slaughtered for carcass and meat evaluation.Results:Final body weight, dry matter intake, average daily gain, and feed conversion ratio were significantly higher in F1-RCA than AW. Hot and cold carcass weights and the other carcass cuts’ weights, except for the fat tail, were heavier in F1-RCA than AW. There was no difference in dressing percentage between the two genetic groups (p>0.05). All non-carcass components’ weights, except spleen, kidney, and testis, were higher in F1-RCA. Total lean, total bone, and intermuscular fat weight were greater in F1-RCA, but bone-to-lean ratio was lower in F1-RCA when compared with AW (p<0.01). No differences (p>0.05) were observed in all meat quality parameters for muscle longissimus with the exception of pH, redness color, and tenderness that were lower (p<0.05) in F1-RCA than AW. F1-RCA lambs had larger longissimus muscle area (30.9 vs. 16.9 cm2) and less leg fat depth (11.1 vs. 17.4 mm).Conclusion:The implications of this research show the potential of 50% of Rambouillet genes and the CLPG mutation to improve growth and meat characteristics in AW-Rambouillet crosses and can be used further to develop a meat-type AW with improved productivity and muscle mass.

Effects of Protein Restriction on Performances and Meat Quality of Cinta Senese Pig Reared in an Organic System.

Simple SummaryFat contained in meat is an important contributor to sensory traits: it increases meat tenderness and flavor. In commercial pigs, increasing meat’s fat was obtained by feeding, until the fattening period, a diet slightly lower in protein respect to their requirements. Local pig breeds, such as Cinta Senese, are known as obese pigs because their great potential to deposit fat, which is mainly stored in backfat deposits. This study was aimed to assess if protein restriction in growing can further increase meat’s fat without alter overall body fatness of obese pigs. The normal feeding management and the restricted one were compared in two groups of Cinta Senese pigs. Results showed that protein restriction during the growing phase affected only few traits. The restricted animal was more able to use the protein in feeding, but few modifications were found in the chemical composition of meat, including the meat’s fat, that remained unchanged. So, obese genotype might be less responsive to this kind of feeding management.In lean genotypes, protein restriction during growing increases intramuscular fat content without affecting the overall carcass fatness. The present study aims to assess the feasibility of applying this feeding management on an obese pig, the Cinta Senese, since obese genotypes are characterized by great lipogenic potential often leading to excessively high backfat deposits. Twenty pigs of average weight 38 kg, were divided in two groups, the first group was fed a protein restricted diet (9% of crude protein), while the second one a normal diet (13.5% of crude protein). During finishing, both groups were fed the same diet (10% of crude protein). Average daily gain, protein conversion index, backfat thickness, carcass weight, and prime cuts were determined. A loin sample joint was dissected in intermuscular fat, bone, subcutaneous fat, longissimus lumborum, and psoas major. On longissimus lumborum, physical and chemical analysis was carried out. The fatty acid profile of longissimus lumborum and loin subcutaneous fat were determined. Data were analyzed by analysis of variance. Protein conversion index resulted lower in the restricted group, while backfat was slightly greater. Meat quality traits were not affected by feeding management. Slightly modifications in subcutaneous outer layer fatty acids profile were observed. The protein restriction during growing did not seem a suitable mode of feeding management for Cinta Senese pigs.

Lumbopelvic Muscle Changes Following Long-Duration Spaceflight.

Long-duration spaceflight has been shown to negatively affect the lumbopelvic muscles of crewmembers. Through analysis of computed tomography scans of crewmembers on 4- to 6-month missions equipped with the interim resistive exercise device, the structural deterioration of the psoas, quadratus lumborum, and paraspinal muscles was assessed. Computed tomography scans of 16 crewmembers were collected before and after long-duration spaceflight. The volume and attenuation of lumbar musculature at the L2 vertebral level were measured. Percent changes in the lumbopelvic muscle volume and attenuation (indicative of myosteatosis, or intermuscular fat infiltration) following spaceflight were calculated. Due to historical studies demonstrating only decreases in the muscles assessed, a one-sample t test was performed to determine if these decreases persist in more recent flight conditions. Crewmembers on interim resistive exercise device-equipped missions experienced an average 9.5% (2.0% SE) decrease in volume and 6.0% (1.5% SE) decrease in attenuation in the quadratus lumborum muscles and an average 5.3% (1.0% SE) decrease in volume and 5.3% (1.6% SE) decrease in attenuation in the paraspinal muscles. Crewmembers experienced no significant changes in psoas muscle volume or attenuation. No significant changes in intermuscular adipose tissue volume or attenuation were found in any muscles. Long-duration spaceflight was associated with preservation of psoas muscle volume and attenuation and significant decreases in quadratus lumborum and paraspinal muscle volume and attenuation.

Effects of dietary N-carbamylglutamate supplementation on growth performance, tissue development and blood parameters of yellow-feather broilers

The effects of N-carbamylglutamate (NCG) on the growth performance, tissue development, and blood parameters of broilers are unknown. In this study, 2 linked experiments were conducted to investigate the effects of 4 graded dietary levels and 3 dietary stages of NCG in a Chinese indigenous yellow-feather broiler breed during 2 growth phases: 1 to 18 d and 19 to 36 d. The dietary levels of NCG were 0.05%, 0.10%, 0.15%, and 0.20%, and dietary stages were designed to add NCG during the starter stage or grower stage or throughout the experimental period. At the age of 18 d, graded doses of NCG from 0.05 to 0.20% in the diet produced quadratic (P < 0.05) positive responses in body weight, width of intermuscular fat cingulum, liver weight, serum blood urea nitrogen, and serum low-density lipoprotein as well as linear (P < 0.05) positive responses in albumin serum concentration. The average feed per gain and mortality were unaffected by dietary NCG levels. Among 3 dietary treatments, only NCG dietary treatments throughout the experimental period improved the body weight and daily weight gain linearly (P < 0.05). The daily weight gain under the 3 dietary treatments used indicated that the most fitting dose is 0.1% NCG among the 4 dietary levels of NCG (P < 0.05). At this dose, muscle weight increased, whereas subcutaneous adipose as well as the serum contents of uric acid, triglyceride, and albumin decreased. Considering the growth performance and tissue development under the conditions used in this study, the best-fit model for NCG requirements of Chinese yellow-feather broilers was estimated from regression analysis to be 0.09 to 0.12% dietary NCG treatments during the grower stage. The modified blood parameters indicated that NCG dietary effects on broiler growth may be accompanied by modified homeostasis of arginine metabolism, lipid deposition, protein synthesis, and immune response.

Influence of metabolic syndrome on thigh intermuscular fat in older adults with knee osteoarthritis

Influence of metabolic syndrome on thigh intermuscular fat in older adults with knee osteoarthritis

Does radiographic knee OA status impact age-related reduction of thigh muscle specific strength - data from the osteoarthritis initiative

Does radiographic knee OA status impact age-related reduction of thigh muscle specific strength - data from the osteoarthritis initiative

Clinical validation of fully automated segmentation of thigh muscle and adipose tissue cross sectional areas using maching learning with a convolutional neural network

Clinical validation of fully automated segmentation of thigh muscle and adipose tissue cross sectional areas using maching learning with a convolutional neural network

Fatty infiltration in the thigh muscles in knee osteoarthritis: a systematic review and meta-analysis.

Knee osteoarthritis is a chronic degenerative joint disease, influenced by inflammatory, mechanical and metabolic processes. Current literature shows that thigh muscles of people with knee osteoarthritis can have increased infiltration of fat, both between and within the muscles (inter- and intramuscular fat). The fatty infiltration in the thigh in this population is correlated to systemic inflammation, poor physical function, and muscle impairment and leads to metabolic impairments and muscle disfunction. The objective of this study is to systematically review the literature comparing the amount of fatty infiltration between people with knee osteoarthritis and healthy controls. A literature search on the databases MEDLINE, Embase, CINAHL SPORTDiscuss, Web of Science and Scopus from insertion to December 2018, resulted in 1035 articles, from which 7 met inclusion/exclusion criteria and were included in the review. All included studies analyzed the difference in intermuscular fat and only one study analyzed intramuscular fat. A meta-analysis (random effects model) transforming data into standardized mean difference was performed for intermuscular fat (six studies). The meta-analysis showed a standardized mean difference of 0.39 (95% confidence interval from 0.25 to 0.53), showing that people with knee osteoarthritis have more intermuscular fat than healthy controls. The single study analyzing intramuscular fat shows that people with knee osteoarthritis have more intramuscular fat fraction than healthy controls. People with knee osteoarthritis have more fatty infiltration around the thigh than people with no knee osteoarthritis. That conclusion is stronger for intermuscular fat than intramuscular fat, based on the quality and number of studies analyzed.

Genetic markers associated with resistance to infectious diseases have no effects on production traits and haematological parameters in Italian Large White pigs

Infectious diseases have large economic impacts on the pig breeding industry worldwide. A few genetic markers associated to disease resistance have been recently identified and used in marker-assisted selection (MAS) in a few pig populations, as part of disease control programs. Neonatal Diarrhea (ND), Post-weaning Diarrhea (PWD), caused by enterotoxigenic E. coli, and Porcine Reproductive and Respiratory Syndrome, caused by PRRS virus (PRRSV), are diseases with high priority for the pig industry. Since interactions or antagonism between growth, innate immunity and disease resistance traits could exist, this work investigated if four disease resistance gene markers [mucin 4 (MUC4), rs338992994 associated with resistance to ND; fucosyltransferase 1 (FUT1), rs335979375 associated with resistance to PWD; and guanylate binding protein 1 (GBP1), rs340943904 and rs80800372 (also known as WUR10000125) associated with resistance to PRRS] could affect seven production traits (average daily gain, back fat thickness, lean meat cuts, feed gain ratio, ham weight, visible intermuscular fat and ham weight loss at first salting) and 15 haematological parameters in about 550 performance tested Italian Large White pigs. We also monitored allele frequencies of the same markers on Italian Large White boars sampled under the national selection breeding program over a 20 years-period. Moreover, we evaluated allele frequencies of these polymorphisms in four Italian local pig breeds (Apulo-Calabrese, Casertana, Cinta Senese and Nero Siciliano). The frequency of the resistance-associated alleles for the four polymorphisms was usually higher in all local pig breeds, indirectly supporting a higher rusticity of autochthonous breeds, compared to commercial populations. The two GBP1 polymorphisms were not in complete linkage disequilibrium in all breeds, except in Apulo-Calabrese. No significant allele frequency change for the investigated markers occurred over 20 years in the Italian Large White boar population in three of these markers. Only FUT1 showed a modest but significant change of allele frequencies over this period. Association analyses carried out in Italian Large White pigs for production traits, meat quality or haematological parameters under investigation showed no significant effect of any genotyped polymorphisms. Our results indicate that implementing MAS programs in Italian Large White pigs with polymorphisms associated with disease resistance have no direct effects on production traits. The selection program running for this heavy pig breed might not negatively impact disease resistance derived by the investigated major genes.

Analysis and preliminary validation of the molecular mechanism of fat deposition in fatty and lean pigs by high-throughput sequencing

Fat deposition in muscle includes intramuscular fat (IMF) and intermuscular fat. IMF content is an index of pork quality; however, because IMF content is difficult to measure in vivo in young animals, conventional breeding for IMF content is difficult to carry out. The mechanism and progression of animal fat deposition is not well understood, and there are currently no effective control methods. In this study, using Laiwu and large white pigs as the research subjects and RNA sequencing technology, we analyzed the genetic mechanism of animal fat deposition in pigs. Specifically, we analyzed the features of lncRNAs and their potential target genes. We obtained 464 million clean reads, from which 907 lncRNAs were identified. The cis and trans analysis identified target genes, including genes that were upregulated (286) and downregulated (621) in the fatty and lean pigs. ENSSSCG00000008692_ADD1, ENSSSCG00000023124_ADD1 and ENSSSCG00000005918_DGAT1 were validated as target genes of the lncRNAs and were shown to be closely related to fat deposition. These results provide a basis for studying the different metabolic lncRNA expression of IMF deposition. In addition, as the valuable model animal to study the mechanisms of obesity, pigs may represent a new avenue for studying human obesity.

Abstract MP58: Intermuscular Fat Density: Novel Risk Factor for Cardiometabolic Risk Factors in African-Ancestry Men

Background: While overall obesity remains a major risk factor for cardiometabolic diseases, the location of stored fat is also thought to play a role. In addition to fat storage in ectopic sites, the density of fat, a marker of fat quality, is emerging as a novel risk factor for cardiometabolic diseases. Previous studies have focused primarily on visceral (VAT) and abdominal subcutaneous (SAT) adipose tissue density and in predominantly white cohorts; however, studies in populations of African ancestry who are at higher risk of cardiometabolic diseases are lacking. Additionally, information is lacking on associations of density of other relevant ectopic fat depots, such as intermuscular adipose tissue (IMAT), with cardiometabolic risk factors. Methods: We investigated whether VAT, SAT, or thigh IMAT densities were associated with cardiometabolic measures in 648 black men aged 50-91 years (mean age 63.6 years, mean BMI 27.7 kg/m 2 ). Adipose tissue volume (cm 3 ) and density (Hounsfield units, HU) was measured in the abdomen (between L4 and L5) and mid-thigh from computed tomography scans. Cardiometabolic measures, including blood pressure, fasting serum glucose and insulin homeostasis, and serum lipids (N=476), were transformed as needed and used as outcomes in multiple linear regression models adjusting for age, lifestyle factors, medication use (antihypertensive, antidiabetic, or lipid-lowering), and corresponding fat depot volume. Results: After adjustment (Table), VAT, SAT, and IMAT densities were significantly associated with fasting insulin, insulin resistance, and lipids. Results remain similar after adjustment for BMI instead of fat volume and in a sensitivity analysis excluding those taking relevant medications. Conclusion: As reported in other studies, in our study among African ancestry individuals VAT and SAT were associated with several cardio-metabolic risk factors. Importantly, our findings suggest that thigh IMAT density may be a novel predictor of cardiometabolic risk in African ancestry men.

Influence of Sex on the Changes in Regional Fat and Skeletal Muscle Mass in Response to Exercise Training in Adolescents with Obesity.

We retrospectively examined the sex differences in the changes in (1) total fat, total and regional subcutaneous adipose tissue (SAT), visceral fat, and intermuscular fat and (2) total and regional skeletal muscle distribution in response to aerobic exercise (AE) or resistance exercise (RE) in adolescents with obesity. Twenty-eight boys and 27 girls with obesity (BMI ≥95th percentile, 12-18 years) were randomly assigned to 3-month interventions (180 minutes per week) of AE or RE. Changes in total and regional fat and skeletal muscle distribution were assessed by a whole-body magnetic resonance imaging. After controlling for corresponding baseline values, age, and race, changes in body weight, BMI, BMI z-score, and waist circumference were similar between exercise groups (p > 0.05) and sexes (p > 0.05). There were no sex or exercise group differences in the reductions in total fat, total SAT, visceral fat, or intermuscular fat. With AE, boys had greater (p < 0.05) reductions in abdominal SAT as compared with girls. With RE, boys had greater (p < 0.05) increases in total, upper body, and abdominal skeletal muscle as compared with girls. Independent of exercise modality, the improvement in VO2max was greater (p < 0.05) in boys than in girls. Independent of sex, the increase in muscular strength index was higher (p < 0.05) in the RE vs. AE group. With the exception of abdominal SAT, there were no sex or exercise treatment differences in the reductions in total and regional fat. In response to RE, the increases in total and regional skeletal muscle were significantly greater in boys than in girls.

Assessment of Subfascial Muscle/Water and Fat Accumulation in Lymphedema Patients Using Magnetic Resonance Imaging.

Background: In patients with arm or leg lymphedema, more fat has been found in the epifascial compartment of the edematous limb compared to the healthy limb. However, not much is known about subfascial fat accumulation in these patients. This study aims to investigate the intramuscular and intermuscular fat and muscle/water volume in lymphedema patients. The excess of intramuscular and intermuscular fat volume was also compared to the excess epifascial fat volume, the excess limb volume, and the duration of lymphedema. Methods and Results: Data from 13 patients (seven arm and six leg lymphedemas) were acquired using a 1.5 T magnetic resonance imaging (MRI) scanner before liposuction and at five time points (4 days, 4 weeks, 3 months, 6 months, and 1 year) after liposuction. From water-fat imaging, fat and muscle/water volumes within the intramuscular and intermuscular compartments were calculated. The relative excess volume was defined as (volume of edematous limb-volume of healthy limb)/volume of healthy limb. Elevated relative excess volumes of intramuscular and intermuscular fat were found at all time points. A decrease in the relative excess volume of muscle/water over time was found. This decrease was not correlated to the relative excess of epifascial fat volume, the relative excess of limb volume, or the duration of lymphedema. Conclusions: An excess fat volume was found in the intramuscular and intermuscular compartments in lymphedema patients. The results suggest that the subfascial compartment needs to be studied separately as no correlation between intramuscular/intermuscular fat accumulation and other measured parameters was found.

Anthropometry Versus Imaging for Prediction of Inflammation Among Hispanic Girls.

ObjectiveTo compare total and regional estimates of body composition, by direct and indirect techniques, for the optimal prediction of CRP among young (9–12 year old), Hispanic girls (N=232).MethodsStandard anthropometric techniques were used to measure height, weight, and waist circumference. Dual energy X-ray absorptiometry (DXA) and peripheral quantitative computed tomography (pQCT) assessed body composition. Fasting serum CRP was measured by the Beckman Coulter AU5812 Clinical Chemistry Analyzer. Associations between each total and regional body composition parameter and CRP were tested using linear regression (log-transformed, continuous CRP) and ordinal logistic regression (CRP < 1.0, ≥1.0–2.9, and ≥ 3.0 mg/L), controlling for maturation, dietary energy, physical activity, and medications.ResultsAll measures of total and regional body fat were positively associated with CRP (P<0.0001), except for intermuscular fat by pQCT. There were no clinically relevant differences in their association with CRP between anthropometric (BMI; waist circumference) and DXA-derived (total fat and regional fat: trunk, gynoid, android fat, leg) measures of fat.ConclusionsMeasurement of body habitus in Hispanic girls, by multiple commonly available means, predicts CRP equally well.

The effect of carcass weight on fatness and muscle and fat colour of male Ojinegra de Teruel light lambs

This study aimed to evaluate the effects of increases in lamb carcass weight in 1-kg steps from 8 to 11 kg on carcass fatness, muscle colour, subcutaneous and renal fat colour, tissue composition of the thoracic limb, and intramuscular fatty acid composition. Sixty-two carcasses from Ojinegra de Teruel male lambs fed concentrates ad libitum and barley straw were used. Both carcass scores and renal fat weight increased with carcass weight (P = 0.0001), but the percentage increase in renal fat weight was twice that of fat scores. Renal fat was prone to store carotenoids earlier than caudal fat, resulting in increased chroma. With increasing carcass weight, muscle colour became less light (decreased L* (P = 0.0001) and an increased in chroma scores (P = 0.001). Increments of 1 kg of carcass weight led to noticeable changes in the M. rectus abdominis colour, except at the increment from 10 kg to 11 kg. Slaughtering lambs at light weights was found to be advisable because renal fat is not a valuable part of the carcass. The lean percentage of the thoracic limb did not increase with carcass weight, as the increased muscle:bone ratio (P = 0.0001) was offset by an increased fat percentage (especially the intermuscular fat %). Changes with increasing carcass weight in the proportions of the main fatty acids in intramuscular fat were small. Moreover, intramuscular fat did not change in quantity or quality. However, when selling carcasses at heavier weights is preferred, achieving carcasses of 11 kg rather than 10 kg was found to be advisable because the deposition of fat in both was similar. Breeds that deposit fat earlier than the breed used in this study should be fed low-energy diets to improve carcass quality. This feeding strategy could also be considered if fat deposition differs between sexes.