Plant-parasitic nematodes (PPNs) pose a significant threat to global agriculture, leading to substantial yield losses. Managing nematode populations remains a challenging option due to financial, technical and environmental constraints. In Ontario, Canada, the presence of Xiphinema nematodes, a virus vector species, is of particular concern. This study reports the discovery of X. diversicaudatum in a peach field in Southern Ontario, Canada. The description includes morphological and molecular characterization using the 18S and 28S rDNA sequences. Morphological and molecular characterization of Ontario’s population matched those from Europe. While X. diversicaudatum was presumed eradicated from Canada, the present finding showed that the nematode could have spread beyond greenhouses. The study highlights the importance of intensive soil sampling for early nematode detection, even as strict reporting requirements may deter such efforts. We report for the first time the presence of X. diversicaudatum on peaches in Canada and in North America.