Piggeries Research Articles

Relationship between growing pig’s housing conditions, behaviours, lesions and health issues under Italian farming system

Low space availability, high temperatures and a barren environment may contribute to the occurrence of abnormal behaviours and lesions in intensive pig farming. The present study evaluated the housing conditions (HCs), that influence behavioural measures (BMs), and lesion and health measures (LHMs) in growing pigs reared in an Italian farming system. Data collection was carried out on two groups of pigs in each farm (Farm A and Farm B), tail docked (DT) and tail undocked (UT). The HCs measured were dry and wet bulb temperature, light, humidity, air quality, average body weight, pen level of cleanliness, space, and feeder front allowance. Light intensity was negatively associated with positive behaviour (coefficient: −0.01; p < 0.001), while the length of the feeder front was positively associated with the proportion of pigs exploring the pen (coefficient: 65.18; p = 0.04). Tail lesion score index (LSI) increased with an increased frequency of negative behaviour (coefficient: 8.05; p < 0.01), increased light intensity (coefficient: 0.29; p < 0.001) and increased proportion of CO2 (coefficient: 498.31; p < 0.001), while it decreased with the average body weight of the pen (coefficient: −4.04; p < 0.001) and the space allowance (coefficient: −198.93; p < 0.001). Finally, UT pigs showed a greater (p < 0.001) tail LSI than DT pigs (126.8 ± 5.71 and 78.5 ± 9.11, respectively for UT and DT pigs). The present study improved the understanding of the effects of HCs, including tail docking, on welfare parameters of growing pigs. HIGHLIGHTS Housing and management conditions might play a prominent role on pig health and welfare parameters during growing period. Social and exploring attitude behaviours affects both ears and tail lesions. Regardless of tail docking, tail injuries were positively associated with negative behaviour, light intensity, and CO2, while they were negatively associated with the average body weight of the pen and the space allowance.

First molecular detection of porcine circovirus type 4 (PCV4) in Malaysia.

Porcine circovirus type 4 (PCV4) is the newest member in the porcine circovirus family, first reported in 2020. To date, the presence of PCV4 has only been reported in China, South Korea and most recently in Thailand. Detection of PCV4 have been reported in various production stages of pigs from piglets, finishers to sows; associated with a myriad of clinical manifestations including porcine dermatitis and nephropathy syndrome (PDNS), postweaning multisystemic wasting syndrome (PMWS), respiratory, enteric and neurological diseases. While successful virus isolation and culture has yet to be reported, pathogenicity of PCV4 has been demonstrated through infectious clone studies. The objective of this study is to investigate the presence of PCV4 in Malaysian porcine population to update the epidemiology of porcine circoviruses in Malaysia. A total of 49 samples from commercial intensive pig farms, abattoir and wild boar population were subjected to conventional polymerase chain reaction assay to detect PCV4 capsid (cap) genome. Resulting cap nucleotide sequences were analyzed for maximum likelihood phylogeny relationship. Results revealed that PCV4 is present in Peninsular Malaysia at a molecular prevalence of 4.08% (2 / 49 samples). Both PCV4 positive samples originated from clinically healthy finishers. Malaysian PCV4 strains were classified as genotype PCV4b, and were found to be phylogenetically distinct from the China, South Korea and Thailand strains. With this latest update of the novel PCV4 in Malaysia, it is clear that more attention needs to be given to the investigation of novel porcine circoviruses (PCV) and management of PCV diseases.

A deep learning-based detection method for pig body temperature using infrared thermography

Body temperature is one of the important indicators that reflect the health of pigs. The traditional rectal temperature measurement is inconvenient and time-consuming, and also easy to cause stress responses to pigs. Infrared Thermography (ITG) was supposed to have potentials to realize non-invasive and rapid temperature detection for pigs in intensive pig farming. In this paper, an automatic temperature detection method base on ITG was developed. First, temperatures on six regions on pig body surface (i.e., forehead (FH), eyes, nose, ear root (ET), back and anus) were measured by ITG for the region of interest (ROI) selection; Then, an improved model of YOLOv5s-BiFPN was developed for automatic ROI detection and temperature extraction. A dataset with 2797 images that collected from sixteen pigs for 30 days was used for model development. It was shown that temperatures on FH and ET were supposed to be the ROI for ITG temperature detection and because of their strong correlations with the rectal temperature among the six parts on pig body surface. Also, the maximum temperature (MaxT) on FH and ET could reflect pig’s body temperature variations the best. The proposed model of YOLOv5s-BiFPN achieved optimal performances (e.g., the mAP was 96.36%, outperformance was 20 MB, target detection speed was up to 100 frame/s), and the mAPs were increased by 4.85%, 4.38%, 1.60%, 1.56%, 31.52%, and 22.42% compared with models of CenterNet, Faster R-CNN, YOLOv4, YOLOv5s, Nanodet and YOLOv5n, respectively. Therefore, it is a feasible way for pig body temperature automatic detection and facilitates early staged disease warning and environmental control.

First report of porcine respirovirus 1 in Brazil

Porcine respirovirus 1 (PRV1), currently referred to as Respirovirus suis, was first described in deceased pigs at a Hong Kong slaughterhouse. Since then, PRV1 strains have been detected in pig herds in American, European, and Asian countries. Considering that Brazil is the fourth-largest global producer and exporter of pork, we aimed to detect the PRV1 RNA in biological samples collected from intensive pig farming in the midwestern region of Brazil. Oropharyngeal and rectal swabs were collected from pigs of different ages at an intensive commercial pig operation. These samples were tested using reverse transcription semi-nested polymerase chain reaction. In this study, the frequency of identification of PRV1 RNA in feces was found to be 2% (1/50), whereas the detection rate of PRV1 in the respiratory mucosa was approximately 1% (1/90). Therefore, a low rate of PRV1 detection was observed only in weaned pigs aged 33–50 days. Sequence analyses revealed that the two Brazilian PRV1 strains were closely related to previously reported strains mainly from Asian countries such as Vietnam, China, and South Korea. These strains clustered with PRV1 sequences classified into the European lineage 1. This is the first report of PRV1 in a commercial pig herd in Brazil. To accurately determine the frequency of detection of PRV1 among pigs in intensive commercial pig farms in Brazil, additional prospective and retrospective studies should be conducted. These studies should aim to detect PRV1 in pig herds with diverse respiratory disease statuses.

Performance investigation of a solar/biomass based multi-generation system in pig farm

Intensive pig farm usually needs a large amount of energy, renewable energy supply system contributes to reducing the reliance of fossil fuel. In this study, load prediction of piggery considering different breeding characteristics and climate condition are carried out. An innovative solar/biomass hybrid multi-generation system combined with biomass combustor, ice thermal energy storage, absorption chiller, evacuated flat plate collector, photovoltaic panel and thermal energy storage is proposed. The operation strategy of following cooling demand is designed for proposed system, which considers the feature of high cooling load of piggery. The influences of key parameters on thermodynamic, economy and environment performances are investigated, thermodynamic operation performances of multi-generation system on different typical days are analyzed. Moreover, non-dominated sorted genetic algorithm is used for tri-objective optimization to obtain the optimal operating parameters and performances. Under the optimal condition, exergy and energy efficiency of integrated system are 19.05% and 53.59%, respectively. Compared with separation system, CO2 emission reduction ratio and dynamic investment payback period of multi-generation system are 70.39% and 5.12 years, respectively. In summary, this study offers a novel way for multiple energy coupling poly-generation system in intensive pig farm so as to obtain optimal performance in operation and scheduling.

Quitting livestock farming: transfarmation pathways and factors of change from post-livestock farmers’ accounts

Transitioning away from livestock farming would limit the carbon footprint of humanity and reduce the pressure on water, land and biodiversity. It would also improve human health, as animal farming increases the risks of pandemics and bacterial resistance. All of these risks and opportunities make a compelling case for a transition towards plant-based diets. In case of a large-scale transition, hundreds of thousands of farmers would have to quit animal farming and switch to other activities. Such transition is potentially happening in developed countries, where industrial operations are located, consumption per capita is the highest and alternatives to animal products are increasingly available. However, there is considerable resistance from farmers to this transition. There is thus a need to better understand potential transition pathways to support smooth transitions. To do so, 27 stories of farm transitioning out of livestock farming – so called transfarmation – were collected. Most of these cases are located in Switzerland and the US. These accounts were published on the websites of organizations that support farmers transitioning out of livestock production or by farmers themselves. In this qualitative study, I coded these accounts to identify patterns in the drivers, behaviour, and decision-making of farmers explaining their transition. Two main patterns were identified: (1) transfarmations from intensive poultry or pig farms towards a mushroom or market gardening farm, driven by economic interests and (2) transfarmations driven by compassion to animals, mostly leading to a farmed animal sanctuary or market gardening farm. Support organizations for transfarmation seem to be particularly beneficial for the second type of transition. I conclude this paper with research perspectives on the topic of transfarmation, especially on the role of gender and the potential of transfarmation for the green care economy.

Carbon and water footprint analysis of pig farm buildings in Northeast China using building-information-modeling enabled assessment

Environmental impact evaluation of buildings is critical for further analysis and optimization of pig farms for sustainable pork production. This study is the first attempt to quantify the carbon and water footprints of a standard intensive pig farm building using building information modeling (BIM) and operation simulation model. The model was constructed with carbon emission and water consumption coefficients, and a database was built. The results showed that the operational stage of pig farm accounted for most of the carbon footprint (49.3–84.9 %) and water footprint (65.5–92.5 %). Building materials production ranked second in carbon (12.0–42.5 %) and water footprints (4.4–24.9 %), and pig farm maintenance ranked third in carbon (1.7–5.7 %) and water footprints (0.7–3.6 %). Notably, the mining and production stages of building materials contributed the largest carbon and water footprints of pig farm construction. Masonry materials have a significant impact on the overall carbon and water footprints of the pig farm. Pig farm using aerated concrete could reduce 41.1 % of the total carbon footprint and 58.9 % of the total water footprint compared to that using coal gangue sintered brick and autoclaved fly ash brick. This study presented a BIM-enabled method for carbon and water footprint analysis of pig farms and illustrated how the model can be used to facilitate the low carbon design of agricultural buildings.

Dietary Berberine and Ellagic Acid Supplementation Improve Growth Performance and Intestinal Damage by Regulating the Structural Function of Gut Microbiota and SCFAs in Weaned Piglets

Early weaning is an effective method for improving the utilization rate of sows in intensive pig farms. However, weaning stress induces diarrhea and intestinal damage in piglets. Berberine (BBR) is known for its anti-diarrhea properties and ellagic acid (EA) is known for its antioxidant properties, however, whether their combination improves diarrhea and intestinal damage in piglets has not been studied, and the mechanism remains unclear. To explore the combined effects in this experiment, a total of 63 weaned piglets (Landrace × Yorkshire) were divided into three groups at 21 days. Piglets in the Ctrl group were treated with a basal diet and 2 mL saline orally, while those in the BE group were treated with a basal diet supplemented with 10 mg/kg (BW) BBR, 10 mg/kg (BW) EA, and 2 mL saline orally. Piglets in the FBE group were treated with a basal diet and 2 mL fecal microbiota suspension from the BE group orally, respectively, for 14 days. Compared with the Ctrl group, dietary supplementation with BE improved growth performance by increasing the average daily gain and average daily food intake and reducing the fecal score in weaned piglets. Dietary supplementation with BE also improved intestinal morphology and cell apoptosis by increasing the ratio of villus height to crypt depth and decreasing the average optical density of apoptotic cells; meanwhile, improvements also involved attenuating oxidative stress and intestinal barrier dysfunction by increasing the total antioxidant capacity, glutathione, and catalase, and upregulating the mRNA expressions of Occludin, Claudin-1, and ZO-1. Interestingly, the oral administration of a fecal microbiota suspension to piglets fed BE had similar effects to those of the BE group. According to 16S rDNA sequencing analysis, dietary supplementation with BE altered the composition of the microbiota, including firmicutes, bacteroidetes, lactobacillus, phascolarctobacterium, and parabacteroides, and increased the metabolites of propionate and butyrate. In addition, Spearman analysis revealed that improvements in growth performance and intestinal damage were significantly correlated with differential bacteria and short-chain fatty acids (SCFAs). In brief, dietary supplementation with BE improved the growth performance and intestinal damage by altering the gut microbiota composition and SCFAs in weaned piglets.

Genome-wide association study reveals candidate genes for pollution excreta traits in pigs.

Excreta traits comprise a very important characteristic in breeding that have been neglected for a long time. With the growth of intensive pig farming, plenty of environment problems have been raised, and people have begun to pay attention to pig excreta behaviors from genetics and breeding perspectives. However, the genetic architecture of excreta traits remains unclear. To investigate the genetic architecture of excreta traits in pigs, eight excreta traits and feed conversion ratio (FCR) were analyzed in this study. We performed genome-wide association studies (GWASs) on 213 Yorkshire pigs and estimated genetic parameters for a total number of 290 pigs, comprising 213 Yorkshire, 52 Landrace and 25 Duroc. After analysis, eight and 22 genome-wide significant SNPs were detected for FCR and the eight excreta traits in single-trait GWASs separately, and 18 were detected in a multi-trait meta-analysis for excreta traits, six of which were detected in both the single-trait and the multi-trait GWAS. Eighty, 182 and 133 genes were detected within 1 Mb of the genome-wide significant SNPs for FCR, excreta traits and multi-trait meta-analysis, respectively. Five candidate genes (BCKDC, DBT, ANKRD7, SHPRH and HCRT) with biochemical and physiological effects relevant to feed efficiency and excreta traits might be interesting markers for future breeding. Meanwhile, functional enrichment analysis indicates that most of the significant pathways are associated with the glutathione catabolic process, DNA topological change and replication fork protection complex. This study reveals the architecture of excreta traits in commercial pigs and offers an opportunity for decreasing the pollution from excreta using genomic selection in pigs.

L'animal et l'homme: De l'exploitation à la sauvegarde ed. by Anne-Marie Flambard-Héricher and François Blary

Reviewed by: L'animal et l'homme: De l'exploitation à la sauvegarde ed. by Anne-Marie Flambard-Héricher and François Blary Violette Pouillard (bio) L'animal et l'homme: De l'exploitation à la sauvegarde Edited by Anne-Marie Flambard-Héricher and François Blary. Paris: Éditions du Comité des travaux historiques et scientifiques, 2021. Pp. 358. This edited collection, which gathers papers around the theme of the exploitation and protection of both domestic and wild animals, is one of several collections of the proceedings of the 141st French National Congress of Historical and Scientific Societies (Comité des travaux historiques et scientifiques) devoted to the theme "Animal and Man." In line with the Congress's multidisciplinary approach, this volume brings together contributions in history, archaeology, archaeozoology, sociology, anthropology, and geography, as well as fields including architecture, information and communication sciences, ethics, and (ethno)pharmacology. The associated diversity of heuristic and methodological issues is reinforced by the broad spectrum of themes, cultural areas, and periods investigated. Thus, if the publication of the proceedings of the annual Congress reflects the current state of the art and research debates, as stated in its aims, this edited collection cannot escape the observation that research in human-animal studies is marked by a deep fragmentation. The exploitation-protection dialectic forms a salutary binding theme; however, the effect is diminished by the absence of introductory remarks for each part of the volume. Finally, though the field of animal history, and more broadly of human-animal studies, has been marked by a profound renewal of research questions for more than a decade, notably around the integration of animals as actors into academic narratives and the forms this integration may take, the book evokes these historiographical debates only marginally. Although the book cannot reflect the current state of the art, it indirectly raises questions about the place and future of the field of animal history and human-animal studies: Is the human-animal issue sufficient to define or institutionally establish a field of research that would be interdisciplinary by nature? How can animal history dialogue with other research fields, and can the discipline exempt itself from addressing historiographical issues that have [End Page 582] influenced adjacent fields such as environmental history (e.g., concerning the social impact of wildlife conservation policies) or the history of science (e.g., the revaluation of empirical knowledge) without running the risk of provincialism or isolation? Finally, could the prominence of controversies regarding the status and condition of animals in (French) academic and public discourse blur the boundaries between academic production and societal debates on the "animal issue," rather than enrich academic production and enhance its specificity and (societal) interest? The defense of the "indispensable nature of animal experimentation" (p. 291) by Jean Costentin and Hubert Vaudry, who write as practitioners and not as researchers in ethics, particularly highlights this issue. Despite the heterogeneous character of the volume, the reader will find in it many interesting contributions, impossible to detail in full here. An important set of contributions focuses on the choices and constraints associated with the use of animals and animal products which, as demonstrated in several articles on medieval (Sarah Claire) and early modern (Opaline Robin and Benoît Clavel) cases, can only be understood in relation to the changing economic, social, and environmental specificities of the time, the study of which requires a localized and fine-tuned approach. Other contributions, in particular that of Mathieu Béghin on intensive pig farming in Amiens during the Middle Ages, reframe the intensification of livestock farming in the long-term and shed light on local to regional remapping of farming geographies resulting from increases in productivity. Catherine Chadefaud and Philippe Madeline both draw attention to the interest of approaching such socio-spatial redistribution through the gestures, techniques, and structures of animal husbandry. In the third part of the book, on the dialectic between protection and destruction, moving from Nicolas Baron's contribution on rabies in cattle, which sheds light on the protective role of vaccines, to Christiane Demeulenaere-Douyère's article on the antivivisection and antivaccination activities of the activist Marie Huot (1846–1930) brings to light the tensions between...

Systematically analysing the acceptability of pig farming systems with different animal welfare levels when considering intra-sustainability trade-offs: Are citizens willing to compromise?

In recent years, intensive pig husbandry has been subject to increasing public criticism, including a clear demand for more animal-friendly housing systems in many countries. However, such systems are associated with trade-offs at the expense of other sustainability domains, which challenges implementation and makes prioritization necessary. Overall, research is scarce that systematically analyses citizens' evaluation of different pig housing systems and associated trade-offs. Given the ongoing transformation process of future livestock systems that meet social demands, it is crucial to include public attitudes. We therefore assessed how citizens evaluate different pig housing systems and whether they are willing to compromise animal welfare in trade-off situations. We conducted an online survey with 1,038 German citizens using quota and split sampling in a picture-based survey design. Participants were asked to evaluate several housing systems with different animal welfare levels and associated trade-offs based on an either positive ('free-range' in split 1) or negative ('indoor housing with fully slatted floors' in split 2) reference system. Initial acceptability was highest for the 'free-range' system, followed by 'indoor housing with straw bedding and outdoor access', 'indoor housing with straw bedding', and 'indoor housing with fully slatted floors', with only the latter being clearly not acceptable for many. Overall acceptability was higher with a positive rather than a negative reference system. When confronted with several trade-off situations, participants became uncertain and temporarily adjusted their evaluations. Thereby participants were most likely to trade off housing conditions against animal or human health rather than against climate protection or a lower product price. Nevertheless, a final evaluation demonstrated that participants did not fundamentally change their initial attitudes. Our findings provide evidence that citizens' desire for good housing conditions is relatively stable, but they are willing to compromise at the expense of animal welfare up to a moderate level.

Pig face recognition based on improved YOLOv4 lightweight neural network

With the vigorous development of intelligence agriculture, the progress of automated large-scale and intensive pig farming has accelerated significantly. As a biological feature, the pig face has important research significance for precise breeding of pigs and traceability of health. In the management of live pigs, many managers adopt traditional methods, including color marking and RFID identification, but there will be problems such as off-label, mixed-label and waste of manpower. This work proposes a non-invasive way to study the identification of multiple individuals in pigs. The model was to first replace the original backbone network of YOLOv4 with MobileNet-v3, a popular lightweight network. Then depth-wise separable convolution was adopted in YOLOv4′s feature extraction network SPP and PANet to further reduce network parameters. Moreover, CBAM attention mechanism formed by the concatenation of CAM and SAM was added to PANet to ensure the network accuracy while reducing the model weight. The introduction of multi-attention mechanism selectively strengthened key areas of pig face and filtered out weak correlation features, so as to improve the overall model effect. Finally, an improved MobileNetv3-YOLOv4-PACNet (M-YOLOv4-C) network model was proposed to identify individual sows. The mAP were 98.15 %, the detection speed FPS were 106.3frames/s, and the model parameter size was only 44.74 MB, which can be well implanted into the small-volume pig house management sensors and applied to the pig management system in a lightweight, fast and accurate manner. This model will provide model support for subsequent pig behavior recognition and posture analysis.

Isolation and identification of two novel pseudorabies viruses with natural recombination or TK gene deletion in China

Pseudorabies virus (PRV), the causative agent of Aujeszky's disease, has gained increased attention in China in recent years due to outbreaks of emergent pseudorabies. However, there is limited information about the evolution and pathogenicity of emergent PRV field strains in China. In this study, two PRV field strains were isolated from an intensive pig farm with suspected PRV infection. These were named the GXLB-2015 and GXGG-2016 strains and their growth characteristics together with their genome sequences and pathogenicity were determined. Nucleotide homology and phylogenetic analysis revealed the GXLB-2015 stain was relatively close to the foreign PRV isolated strains with respect to the whole genome sequence. However, it formed an independent branch between the foreign PRV isolates and the previous PRV variants isolated in China. Further recombination and genetic evolution analysis showed that the GXLB-2015 strain was a natural recombinant between the Bartha strain and PRV variants. The GXGG-2016 strain was highly homologous with the Chinese classical strains, but it has a natural deletion of 69 aa in the thymidine kinase (TK) gene. Pathogenicity analysis showed that, the GXLB-2015 strain had the strongest pathogenicity to mice with an LD50 of 103.5, while the GXGG-2016 strain with the TK gene deletion was not pathogenic to mice. Taken together, our data provide direct evidence for the genomic recombination and natural TK gene deletion of PRVs, which may provide a reference for a better understanding of PRV evolution in China and contribute to the clinical control of PRV infection in pig farms.

A Serological Investigation of Porcine Reproductive and Respiratory Syndrome and Three Coronaviruses in the Campania Region, Southern Italy.

Porcine coronaviruses and reproductive and respiratory syndrome (PRRS) are responsible for severe outbreaks that cause huge economic losses worldwide. In Italy, three coronaviruses have been reported historically: porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV). Although repeated outbreaks have been described, especially in northern Italy, where intensive pig farming is common, there is a worrying lack of information on the spread of these pathogens in Europe. In this work, we determined the seroprevalence of three porcine coronaviruses and PRRSV in the Campania region, southern Italy. A total of 443 samples were tested for the presence of antibodies against porcine coronaviruses and PRRSV using four different commercial ELISAs. Our results indicated that PEDV is the most prevalent among porcine coronaviruses, followed by TGEV, and finally PRCV. PRRSV appeared to be the most prevalent virus (16.7%). For coronaviruses, seroprevalence was higher in pigs raised in intensive farming systems. In terms of distribution, TGEV is more widespread in the province of Avellino, while PEDV and PRRSV are more prevalent in the province of Naples, emphasizing the epidemic nature of both infections. Interestingly, TGEV-positive animals are more common among growers, while seropositivity for PEDV and PRRSV was higher in adults. Our research provides new insights into the spread of swine coronaviruses and PRRSV in southern Italy, as well as a warning about the need for viral surveillance.

Metagenomics analysis reveals potential pathways and drivers of piglet gut phage-mediated transfer of ARGs

The growing prevalence of antibiotic-resistant pathogens has led to a better understanding of the underlying processes that lead to this expansion. Intensive pig farms are considered one of the hotspots for antibiotic resistance gene (ARG) transmission. Phages, as important mobile carriers of ARGs, are widespread in the animal intestine. However, our understanding of phage-associated ARGs in the pig intestine and their underlying drivers is limited. Here, metagenomic sequencing and analysis of viral DNA and total DNA of different intestinal (ileum, cecum and feces) contents in healthy piglets and piglets with diarrhea were separately conducted. We found that phages in piglet ceca are the main repository for ARGs and mobile genetic element (MGE) genes. Phage-associated MGEs are important factors affecting the maintenance and transfer of ARGs. Interestingly, the colocalization of ARGs and MGE genes in piglet gut phages does not appear to be randomly selected but rather related to a specific phage host (Streptococcus). In addition, in the feces of piglets with diarrhea, the abundance of phages carrying ARGs and MGE genes was significantly increased, as was the diversity of polyvalent phages (phages with broad host ranges), which would facilitate the transfection and wider distribution of ARGs in the bacterial community. Moreover, the predicted host spectrum of polyvalent phages in diarrheal feces tended to be potential enteropathogenic genera, which greatly increased the risk of enteropathogens acquiring ARGs. Notably, we also found ARG-homologous genes in the sequences of piglet intestinal mimiviruses, suggesting that the piglet intestinal mimiviruses are a potential repository of ARGs. In conclusion, this study greatly expands our knowledge of the piglet gut microbiome, revealing the underlying mechanisms of maintenance and dissemination of piglet gut ARGs and providing a reference for the prevention and control of ARG pollution in animal husbandry.

Genomic Insights into the Mobilome and Resistome of Sentinel Microorganisms Originating from Farms of Two Different Swine Production Systems.

Antimicrobial resistance (AMR) is a threat to public health due to long-term antimicrobial use (AMU), which promotes the bacterial acquisition of antimicrobial resistance determinants (ARDs). Within food-producing animals, organic and extensive Iberian swine production is based on sustainable and eco-friendly management systems, providing an excellent opportunity to evaluate how sustained differences in AMU impact the development and spread of AMR. Here, through a whole-genome sequencing approach, we provide an in-depth characterization of the resistome and mobilome and their interaction in 466 sentinel bacteria, namely, Escherichia coli, Enterococcus spp., Campylobacter coli, and Staphylococcus spp., recovered from 37 intensive and organic-extensive pig farms. Both ARDs and mobile genetic elements (MGEs) were primarily taxon-associated, with higher similarities among bacteria which were closely phylogenetically related. E. coli exhibited the most diverse resistome and mobilome, with 85.4% mobilizable ARDs, 50.3% of which were plasmid-associated. Staphylococcus spp. exhibited a broad repertoire of ARDs and MGEs, with 52.3% of its resistome being mobilizable. Although Enterococcus spp. carried the highest number of ARDs per isolate and its plasmidome was similar in size to that of E. coli, 43.7% of its resistome was mobilizable. A narrow spectrum of ARDs constituted the C. coli resistome, with point mutations as its main AMR driver. A constrained AMU, as observed in organic-extensive herds, determined a reduction in the quantitative composition of the resistome and the complexity of the resistome-mobilome interaction. These results demonstrate taxon-associated AMR-MGE interactions and evidence that responsible AMU can contribute to reducing AMR pressure in the food chain. IMPORTANCE This study provides the first integral genomic characterization of the resistome and mobilome of sentinel microorganisms for antimicrobial resistance (AMR) surveillance from two different swine production systems. Relevant differences were observed among taxa in the resistomes and mobilomes they harbored, revealing their distinctive risk in AMR dissemination and spread. Thus, Escherichia coli and, to a lesser extent, Staphylococcus spp. constituted the main reservoirs of mobilizable antimicrobial resistance genes, which were predominantly plasmid-associated; in contrast to Campylobacter coli, whose resistome was mainly determined by point mutations. The reduced complexity of mobilome-resistome interaction in Enterococcus spp. suggested its limited role in AMR dissemination from swine farms. The significant differences in antimicrobial use among the studied farms allowed us to assess the suitability of whole-genome sequencing as a rapid and efficient technique for the assessment of mid- to long-term on-farm interventions for the reduction of antimicrobial use and the evaluation of AMR status.

Cystoisospora suis infection in suckling piglets in Brazil: Prevalence and associated factors.

Piglet coccidiosis is a parasitic disease caused by the protozoan Cystoisospora suis, which is regarded as the most prevalent gastrointestinal parasite in intensive pig farms. Despite the availability of highly effective chemo-metaphylaxis (toltrazuril), coccidiosis is still prevalent in European and other countries. We conducted a cross-sectional study on swine farms in Brazil, to assess the prevalence of Cystoisospora suis in fecal samples and determined the associated factors. In total, 666 litters from 50 farms were sampled twice within one week between samplings (mean age at sampling: 10.75 and 17.7days). Of 666 litters, 225 (33.8%) were positive at least once, and the expected within farm prevalence of C. suis oocysts was 32.9% (25.4-41.3%; 95% confidence interval). Oocysts were more prevalent in fecal samples collected from farms with diarrhea (odds ratio=6.75). The room temperature was also positively associated with oocyst detection; a one-degree increase in room temperature increased the chance of a litter being positive by 23.2%. Up-to-date, this is the most comprehensive technical evidence of factors associated with C. suis infection in Brazilian industrial piglet farms.

Serum investigation of antibodies against porcine circovirus 4 Rep and Cap protein in Jiangxi Province, China.

In 2019, a novel porcine circovirus 4 (PCV4) was first identified in Hunan Province, China. The circular PCV4 DNA was detected in both diseased and healthy pigs. Recently, PCV4 prevalence surveys have been analyzed in many provinces in both China and South Korea with low positive rates. However, no serological data has been conducted to investigate the prevalence of PCV4 in pigs from Jiangxi Province. To address this issue, an indirect anti-PCV4 antibody enzyme-linked immunosorbent assay (ELISA) based on Cap and Rep protein as a coating antigen was established and applied to study the serum epidemiology of PCV4 in Jiangxi Province. Purified PCV4-His-tagged Cap and Rep were used as the coating antigen to develop an ELISA detection kit. There was no cross-reaction of the Cap/Rep-based ELISA with antisera against PCV2, TGEV and PRRSV, indicating a high specificity of this ELISA assay. The intra-assay coefficient variations (CVs) of Cap-based were 1.239%-9.796%, Rep-based 1.288%-5.011%, and inter-assay CVs of 1.167%-4.694% and 1.621%-8.979%, respectively, indicating a good repeatability. Finally, a total number of 507 serum samples were collected from Jiangxi Province to test for antibody prevalence of PCV4, and 17 (3.35%) and 36 (7.10%) of the samples were Cap and Rep antibody positive, respectively. In summary, our established ELISA kit could be used to detect PCV4 antibodies in serum with good repeatability and high specificity. In addition, field samples detection results showed that the antibody of PCV4 was poorly distributed in intensive pig farms in Jiangxi Province, China.

Farming Intensification in Northern Ireland – A State-Corporate Environmental Harm?

Food production may involve serious harms that lie beyond traditional definitions of crime and are not statutorily proscribed. One example of a criminologically under-researched source of harms is intensive farming. Taking a case study of rising intensive pig farming in Northern Ireland, this paper innovatively applies the state-corporate crime framework to analyse the catalysts for environmental and social harm in the country, expanding the knowledge of complex relationships between political and economic actors from a green criminological perspective and further advancing the agenda of ‘greening’ of state-corporate crime (Bradshaw, 2014). It concludes that a state-corporate symbiosis supports and reinforces a market-oriented, profit-driven model of farming that prioritises efficiency and ultimately leads to ‘lawful but awful’ intensification.

Ammoniacal nitrogen recovery from pig slurry using a novel hydrophobic/hydrophilic selective membrane

The implementation of the circular economy paradigm in intensive pig farming requires technologies able to recover ammoniacal nitrogen from pig slurries. This research explores the feasibility of a novel hydrophobic/hydrophilic non-porous membrane to recover ammoniacal nitrogen from pig slurry at ambient temperature and using a H2SO4 solution as trapping agent. The influence of (i) the pH of the feed solution, (ii) the volume ratio between feed and trapping solution, and (iii) the trapping solution concentration on nitrogen recovery and flux were evaluated using a synthetic solution and pig slurry. The best performance was achieved when the pH of the feed solution was controlled at 9.0, where average fluxes of 145 and 116 g N/(m2·day) were achieved for the synthetic solution and pig slurry after 24 h, respectively. Decreasing the feed-to-trapping volume ratio improved the recovery efficiency after 24 h from 62% to 74% for the synthetic solution and from 32% to 46% for pig slurry. However, renewing the H2SO4 concentration of the trapping solution only led to minor improvements despite the higher reagent consumption. The diffusion coefficients of NH3 and NH4+ through the membrane at pH 9.0 were (7.3 ± 0.2)·10–11 and (2.1 ± 0.1)·10–11 m2/s for the synthetic solution and (2.7 ± 0.1)·10–11 and (1.0 ± 0.1)·10–11 m2/s for the pig slurry, respectively. The capacity of ions to diffuse through the membrane is a distinctive feature of this membrane and allowed recovering 33% of potassium and 21% of phosphate in pig slurry after 24 h.