Nursery material intended for establishing intensive apple orchards should be characterised by a dominant and straight leader with an appropriate number of shoots that develop at the right height and are regularly spaced along the leader. The use of well-branched trees can lead to fruiting in the first year after planting. However, many apple varieties have difficulty forming lateral shoots due to strong apical dominance. The aim of the study was to assess the effectiveness of treatments stimulating the branching of maiden apple trees of the ‘Gloster’ variety. The research was carried out in 2017–2019 at a private nursery farm located in eastern Poland. The studied trees were subjected to a mechanical branching stimulation treatment, which consisted of pinching off 4–5 of the youngest leaves located below the growth cone, and chemical branching stimulation treatments, which consisted of applying growth regulator mixtures in the form of an aqueous solution, i.e., BA+GA3 and BA+GA4+7. The conducted studies showed that the type of branching-stimulating treatment had a significant effect on the height and trunk diameter of the maiden trees, the number of lateral shoots, the average length of one shoot and the sum of the lengths of all sylleptic shoots. The maiden trees treated with BA+GA3 were characterised by the best quality among the analysed combinations. Maiden apple trees treated with BA+GA3 were the tallest (2017—167.7 cm; 2018—175.3 cm; 2019—164.4 cm), produced the largest number of shoots (2017—6.5 pcs; 2018—6.8 pcs; 2019—6.3 pcs) and had the largest sum of lateral shoot lengths (2017—148.0 cm; 2018—155.4 cm; 2019—140.6 cm) among the evaluated combinations. The number of treatments and the concentration of applied growth regulators had a significant effect on the structure of the crown of the maiden apple trees of the ‘Gloster’ cultivar.