Event Abstract Back to Event Effect of clock genes deletion on the structure of the Retina Ouafa Ait-Hmyed1, 2*, Guy Normand1, Mohamed Bennis2 and David Hicks1 1 CNRS UPR 3212, INCI, Department of Neurobiological Rhythms, France 2 Laboratory of Pharmacology, Neurobiology and behaviour, UFR Biology and Health, Department of Biology, Faculty of Sciences, Morocco Purpose: Many retinal activities are regulated by an endogenous circadian clock. We thus examined the retinal phenotype of clock gene knockout mice to search for possible modifications.Methods: In one series, eyes were obtained from adult wild type (Bl/6), per1 –/–, per2 –/–, and per1–/–/per2–/– mice, and fixed for sectioning. Eyes were processed for conventional histology, or frozen sections for immunohistochemistry using a battery of cell markers. A second series of each mouse strain were adapted to a standard 12h light/12 h dark cycle, one group was exposed to intense white light, one group was killed 1h after lights on (zeitgeber time 1, ZT1). Some retinas were taken for western blotting. Results: Measures of cell density or layer thickness were similar between all strains. Qualitative images of rod (rhodopsin, transducin), cone (MW opsin, cone arrestin) and other retinal neuronal markers were also similar between all samples. Immunoblotting of rod and cone proteins showed no differences. Exposure to intense light did not induce photoreceptor damage in any strain. Finally, quantification of phagosomes showed equal numbers in all strains. Conclusions: Deletion of either per1 or per2 or both genes does not lead to any obvious loss in a given cell type, or affect retinal physiopathology. Conference: 3rd Mediterranean Conference of Neuroscience , Alexandria, Egypt, 13 Dec - 16 Dec, 2009. Presentation Type: Poster Presentation Topic: Imaging & Neuroendocrinology Citation: Ait-Hmyed O, Normand G, Bennis M and Hicks D (2009). Effect of clock genes deletion on the structure of the Retina. Front. Neurosci. Conference Abstract: 3rd Mediterranean Conference of Neuroscience . doi: 10.3389/conf.neuro.01.2009.16.166 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Nov 2009; Published Online: 26 Nov 2009. * Correspondence: Ouafa Ait-Hmyed, CNRS UPR 3212, INCI, Department of Neurobiological Rhythms, Strasbourg, France, rebaiouafa@yahoo.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ouafa Ait-Hmyed Guy Normand Mohamed Bennis David Hicks Google Ouafa Ait-Hmyed Guy Normand Mohamed Bennis David Hicks Google Scholar Ouafa Ait-Hmyed Guy Normand Mohamed Bennis David Hicks PubMed Ouafa Ait-Hmyed Guy Normand Mohamed Bennis David Hicks Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.