Tissue Integrity Research Articles

Native tissue repair of the female pelvic floor: A four-level surgical concept.

This review describes our experience with native tissue repair of the visceral pelvic fascia, the perineum, and anal sphincters in women. We propose that complex repair of the pelvic floor should consider vaginal support in all three anatomical Delancey's levels, together with more caudal structures-the external and internal anal sphincters. Original illustrations were created to facilitate the understanding of the complex anatomy of common multi-level defects. As the integrity of connective tissue adds to various aspects of the delicate function of the female pelvic floor, it is complete and as perfect as possible repair is a common goal of both gynecologists and colorectal specialists.

Fraction and Antibacterial Activity of Bitter Melons (Momordica charantia) in Koi Fish (Cypinus carpio) Infected with Aeromonas salmonicida Bacteria and its Effect on Gills Histopathology

Koi fish (Cyprinus carpio) is a type of freshwater ornamental fish with significant economic value, attracting widespread public interest due to its vibrant colors and agile movements. Therefore, this study introduces a novel approach by evaluating the antibacterial activity of bitter melon extract fraction (Momordica charantia) against A. salmonicida infection in koi fish and its impact on gill histopathology. The novelty of this study lies in the exploration of bitter melon extract fraction as a natural, eco-friendly antibacterial alternative to synthetic antibiotics in aquaculture. This research is crucial as it addresses the growing global concern over antibiotic resistance and the urgent need for sustainable, natural solutions in fish health management. Antibacterial tests were conducted to assess the ability of the bitter melon extract to inhibit bacterial growth, while histopathological analysis aimed to observe the specific changes in gill tissues of infected koi fish. The results revealed that the bitter melon extract fraction significantly reduced bacterial count and improved gill tissue integrity. Histopathological improvements were observed, including reduced hyperplasia, lamellar fusion, edema, and necrosis, indicating tissue recovery. This finding highlights the potential of bitter melon extract fraction as a natural antibacterial agent for controlling bacterial infections in fish, offering an alternative to chemical antibiotics. Further research is needed to determine the optimal dosage and to explore its antibacterial mechanism, ensuring its safe and effective application in aquaculture.

Laparoscopic repair of inguinal hernia using GORE SYNECOR biomesh

Inguinal hernia repair, historically performed through open surgery, has evolved to favour laparoscopic techniques due to reduced recovery time, minimized pain, and lower recurrence rates. The type of mesh used in repair plays a crucial role in surgical outcomes. Synthetic meshes are known for their durability and tensile strength, while biological meshes offer better biocompatibility and reduced risk of chronic inflammation. Recently, hybrid meshes such as GORE SYNECOR have emerged, integrating the strengths of both types. These composite meshes provide structural support while promoting tissue integration, yielding promising results in terms of low recurrence rates and improved patient-reported outcomes post-surgery. This report discusses an 81-year-old male with a left-sided indirect inguinal hernia managed through laparoscopic transabdominal preperitoneal (TAP) repair, utilizing GORE SYNECOR biomesh. His clinical presentation, surgical course, and recovery highlight the effectiveness of hybrid meshes for elderly patients, offering both durability and reduced complication risks. The use of a hybrid composite intraperitoneal device like the GORE SYNECOR mesh for inguinal and abdominal hernia repair demonstrates encouraging early outcomes. These include low recurrence rates, a strong safety profile, and notable enhancements in patients' health-related quality of life extending beyond one year.

The survival grip-how cell adhesion promotes tumor maintenance within the microenvironment.

Cell adhesion is warranted by proteins that are crucial for the maintenance of tissue integrity and homeostasis. Most of these proteins behave as receptors to link adhesion to the control of cell survival and their expression or regulation are often altered in cancers. B-cell malignancies do not evade this principle as they are sustained in relapsed niches by interacting with the microenvironment that includes cells and their secreted factors. Focusing on chronic lymphocytic leukemia and mantle cell lymphoma, this Review delves with the molecules involved in the dialog between the adhesion platforms and signaling pathways known to regulate both cell adhesion and survival. Current therapeutic strategies disrupt adhesive structures and compromise the microenvironment support to tumor cells, rendering them sensitive to immune recognition. The development of organ-on-chip and 3D culture systems, such as spheroids, have revealed the importance of mechanical cues in regulating signaling pathways to organize cell adhesion and survival. All these elements contribute to the elaboration of the crosstalk of lymphoma cells with the microenvironment and the education processes that allow the establishment of the supportive niche.

A IMPORTÂNCIA DA SUPLEMENTAÇÃO NUTRICIONAL PARA A PREVENÇÃO E RECUPERAÇÃO DE LESÕES EM ATLETAS: UMA REVISÃO BIBLIOGRÁFICA

Sports nutrition is an area dedicated to the study and application of nutritional principles with the aim of improving the physical performance of athletes, in addition to helping to prevent and recover from injuries. This work investigates how sports nutrition contributes to preventing and recovering from injuries, analyzing nutrients that maintain tissue integrity, reduce risk and accelerate healing. Material and methods: A survey of studies was conducted in the Scielo, Google Scholar, Pubmed, Lilacs databases, published between the years 2015 and 2024, capturing 10 articles that met all inclusion criteria. Results: Of the 10 studies analyzed, 8 demonstrated positive results when using a protein dosage between 1.6 and 2.5 g/kg. Regarding the use of supplements, 8 studies indicated beneficial effects, while 2 presented inconclusive results. Thus, only 8 studies showed improvements in injury recovery. Conclusions: The results of this research demonstrated that sports nutrition plays a fundamental role in preventing and recovering from sports injuries, in addition to a balanced and diversified diet providing the necessary substrates for carrying out physical activities.

Evaluation of artificial intelligence and optical image recognition techniques used in OneIso, an off-axis Winston-Lutz quality assurance phantom.

Single-isocenter multitarget (SIMT) stereotactic-radiosurgery (SRS) has recently emerged as a powerful treatment regimen for intracranial tumors. With high specificity, SIMT SRS allows for rapid, high-dose delivery while maintaining integrity of adjacent healthy tissues and minimizing neurocognitive damage to patients. Highly robust and accurate quality assurance (QA) tests are critical to minimize off-targets and damage to surrounding healthy tissues. We have developed a novel QA phantom, named OneIso, to accurately and precisely measure off-axis accuracy, via off-axis Winston-Lutz (OAWL), to assist SIMT SRS programs. In this study, a comparison of three different quantitative numerical methods were performed for isolating and measuring the position of ball-bearings (BBs) used in the OAWL measurement. The three methods evaluated were: 1) feature extraction technique combined with manual intervention 2) a proprietary software utilizing optical image recognition (OIR) techniques, and 3) a machine learning (ML) model employing convolutional neural networks (CNNs). These methods were used to analyze OAWL datasets gathered from a OneIso phantom deployed on a Varian TrueBeam. The precision of localizing positional BBs within the OneIso QA phantom, analysis speed, and robustness were compared across the methods. Significantly, the trained ML model utilizing CNNs was found to exhibit superior precision, analysis speed, and efficiency compared to the other two methods. These results highlight the benefit in shifting from manual and OIR methods to that of ML techniques. The incorporation of CNNs in automated QA analysis can achieve improved precision, allowing for more rapid and wider adoption of SIMT SRS for treating intracranial metastases while preserving integrity of surrounding healthy tissues.

Synergistic Enhancement of Antibacterial and Osteo-Immunomodulatory Activities of Titanium Implants via Dual-Responsive Multifunctional Surfaces.

Bone implant-associated infections and inflammations, primarily caused by bacteria colonization, frequently result in unsuccessful procedures and pose significant health risks to patients. To mitigate these challenges, the development of engineered implants with spatiotemporal regulation capabilities, designed to inhibit bacterial survival and modulate immune responses in the early stage, while promoting bone defect healing in the late stage is proposed. The implants are functionalized with ε-poly-l-lysine-phenylboronic acid (PP) via dynamic boronic ester bonds, which facilitate its release through a reactive oxygen species (ROS) and pH-responsive strategy, thereby establishing an antibacterial microenvironment on and around the implants. Additionally, the dynamic metal coordination interaction facilitates the loading and sustained release of Sr2+ under an acidic environment, providing immunomodulatory and osteogenic effects. The ROS/pH-responsive feature, coupled with the implant-bone tissue integration process, affords precise spatiotemporal regulation of the Ti-TA-Sr-PP implants. This strategy represents a promising approach for the preparation of advanced bone implants.

Arthroscopic ACL repair with suture tape augmentation: clinical, functional, and gait analysis outcomes at minimum 3-year follow-up.

Recent advancements in surgical techniques have led to renewed interest in ACL repair, particularly for acute, proximal tears. Suture tape augmentation (STA) has emerged as a promising technique to support ACL healing while preserving native tissue and potentially improving outcomes. This study aims to evaluate the outcomes of ACL repair with STA in patients with acute, proximal ACL tears. We retrospectively reviewed patients who underwent ACL repair with STA between June 2018 and October 2020. Inclusion criteria were acute (< 6weeks) ACL rupture, Sherman type I tears, and minimum follow-up of 3years. Exclusion criteria included mid-substance or distal ACL tears and previous knee surgeries. Clinical evaluation involved stability tests, PROMs, and gait analysis using Prokin WalkerView. Second-look arthroscopy and histological analysis were conducted on a subset of patients. Twelve consecutive patients (7 males and 5 females) with a mean age of 27.4 ± 7.9years and mean BMI of 23.3 ± 1.6kg/m2were included. Time to operation was 19.5 ± 8.7days. Mean follow-up duration was 53.2 ± 9.4months. One patient experienced a traumatic re-rupture. One had a contralateral ACL tear and one experienced hardware irritation. Clinical tests were negative for instability in all patients. Post-operative IKDC, Lysholm, and FJS-12 scores showed significant improvement (p < 0.001). All patients (100%) achieved minimal clinically important difference for all PROMs. Walking gait analysis revealed high symmetry indices for range of motion (93.50%) and step length (95.80%) with near symmetrical loading. Second-look arthroscopy showed intact repairs with healthy tissue morphology and integration. Histology revealed increased cellularity, high nuclear density, and preservation of vascular and neural components indicated by CD34 and S-100 markers. Arthroscopic ACL repair with STA provides favourable clinical, functional, and histological outcomes with low re-rupture rates when performed on acute, proximal ACL tears. This technique demonstrates fair-to-good PROMs, functional stability, and near-normal gait parameters.

Dynamic cell fate plasticity and tissue integration drive functional synovial joint regeneration.

Adult mammalian synovial joints have limited regenerative capacity, where injuries heal with mechanically inferior fibrotic tissues. Here we developed a unilateral whole-joint resection model in adult zebrafish to advance our understanding of how to stimulate regrowth of native synovial joint tissues. Using a combination of microCT, histological, live imaging, and single-cell RNA sequencing (scRNAseq) approaches after complete removal of all joint tissues, we find de novo regeneration of articular cartilage, ligament, and synovium into a functional joint. Clonal lineage tracing and scRNAseq implicate a multipotent, neural crest-derived population in the adult skeleton as a cell source for these regenerating tissues. Together, our findings reveal latent molecular and cellular programs within the adult skeleton that are deployed to regenerate a complex joint with lubricated articular cartilage.

A Comprehensive Study on the Renal Protective Effects of Polyherbal Extract Formulation and Their Impact on Kidney Function in Rats

Background: The nephroprotective effects of herbal extracts have increasingly gained attention due to the rise in kidney diseases and the limitations of conventional treatments. This study explores the renal protective properties of a polyherbal extract formulation composed of Ziziphus spina-christi, Trigonella foenum-graecum and Nigella sativa (ZTN) in Wistar albino rats. Methods: Three groups of adult male and female Wistar albino rats consisting of the control group that received only water, another group was administered with 100 mg/kg of the polyherbal (ZTN herbal) formulation, while the last group was administered with 250 mg/kg of the ZTN polyherbal extract formulation. The treatments administered for 14 days and thereafter Kidney weight, uric acid, Blood Urea Nitrogen (BUN) and creatinine levels were assessed to assess renal function. Histological analysis was conducted for tissue integrity evaluation. Result: The polyherbal extract formulation of ZTN induced a dose-dependent increase in kidney weight in both male and female rats. Uric acid levels decreased significantly in a dose-dependent manner, with the most substantial reduction at 250 mg/kg. BUN levels also showed a notable decrease, particularly at the higher dose, indicating enhanced renal function. Creatinine levels exhibited a significant dose-dependent reduction, with female rats showing higher baseline levels but greater overall reductions. Histological analysis revealed mild histological changes at 100 mg/mL and severe changes at 250 mg/mL. Histological changes include glomerular hypertrophy and tubular necrosis, more pronounced at higher doses. The polyherbal extract formulation of ZTN demonstrated significant nephroprotective effects, reducing uric acid, BUN and creatinine levels in a dose-dependent manner. Interestingly, an observation of gender-based differences occur in female rats showing higher baseline levels but greater reductions with treatment. These findings support the potential therapeutic use of polyherbal extracts in managing renal health, emphasizing the importance of dose regulation to avoid adverse effects.

Oculofacial plastic surgery in the cancer patient: A narrative review.

The authors performed a computerized search using PubMed, Embase, and Google Scholar. The search terms used were "chemotherapy AND surgery", "immunotherapy AND surgery", "radiotherapy AND surgery", "nutrition AND surgery", "(oculoplastic OR orbit OR eyelid OR lacrimal OR puncta) AND (chemotherapy OR immunotherapy OR radiotherapy)", "(facial OR facial plastic OR oculoplastic) AND (chemotherapy OR immunotherapy OR radiotherapy)", "(cancer OR malignancy) AND surgery", "(cancer OR malignancy) AND (surgery OR surgical) complications", "wound healing AND (cancer OR malignancy)", "infection AND (cancer OR malignancy)", "(bleeding OR blood loss) AND (cancer OR malignancy) AND surgery", "(chemotherapy OR immunotherapy OR radiotherapy) AND wound healing", "(chemotherapy OR immunotherapy OR radiotherapy) AND (bleeding OR blood loss)", "(chemotherapy OR immunotherapy OR radiotherapy) AND infection". A total of 89 articles, published from 1993 to 2023 in the English language or with English translations were included. Articles published earlier than 2000 were cited for foundational knowledge. References cited in the identified articles were also used to gather further data for the review. Patients who are being treated for cancer or are undergoing current treatment for cancer require special considerations. Systemic therapies and radiotherapy impact the physiology of patients and the integrity of tissue in ways that significantly impact surgical interventions. It is imperative for the oculofacial plastic surgeon to have a complete understanding on how a previous or current diagnosis of cancer can influence surgical outcomes.

Optimizing Osteotomy Techniques: Comparison of Conventional Rotary Instruments Versus Other Bone Cutting Devices.

Osteotomy procedures can be performed using conventional rotary instruments or piezoelectric surgery. Each technique has distinct advantages and disadvantages regarding surgical precision, patient outcomes, and recovery. To systematically review the literature comparing conventional rotary instruments and piezoelectric surgery in osteotomy procedures. PubMed, Cochrane Library, and Embase were systematically searched. Studies were included if they compared outcomes of conventional rotary instruments and piezoelectric surgery in osteotomy. Data on postoperative pain, swelling, surgical time, and other relevant outcomes were extracted. Thirteen studies met the inclusion criteria. Findings indicated no significant difference in postoperative swelling between the 2 techniques, whereas piezoelectric surgery was associated with reduced postoperative pain and improved tissue integration but required longer surgical times. Piezoelectric surgery shows potential benefits over conventional rotary instruments in specific contexts but requires longer operative times.

Are spinal cord and medulla samples from embalmed donors suitable for histological examination? A pilot study.

A general belief exists that tissue from anatomical donors, especially from the central nervous system (CNS), may not be suitable for histological investigation. This is based on the idea that fixation routinely used in embalming whilst optimal for enabling dissection, insufficiently preserves tissue architecture at the cellular level. However, anatomical donors represent a precious resource for microscopical investigation, provided that preservation is sufficient to enable recognition of structures at the histological level. This could considerably extend the biomedical knowledge currently gained from donations. In this study, we addressed this question and examined histologically, samples of medulla and spinal cord from donors from the Anatomical Gift Programme of RCSI in Dublin. Samples of medulla and spinal cord in the cervical, thoracic and lumbar regions were obtained with appropriate permissions from four different donors that had been previously embalmed for routine anatomical examination. The tissue obtained was processed for paraffin embedding and routine histology. Analysis revealed that several features were identifiable with good histological detail. A scoring system was applied to evaluate the level of histological preservation in the various samples by assessing the following parameters: tissue integrity, presence of leptomeninges along the entire section surface, distinction/adherence grey/white matter, identification of neurons, other cell nuclei, neuropil pattern, capillaries and other intraneural vessels. Lumbar spinal cord and to a lesser extent medulla and cervical spinal cord showed on average good quality of preservation. Conversely, thoracic spinal cord tended, in general, to be less well preserved. We can conclude that spinal cord and medulla samples from embalmed donors can be suitable for histological examination. These findings open new avenues of study of spinal cord and brain stem anatomy on cadaveric tissue from donors already existing in anatomy donation programmes. These results also pave the way for the potential development of new human models of study useful to spinal cord and brain stem researchers. Finally, this expands considerably the impact of the generosity of anatomical donors for the advancement of biomedical knowledge.

Tissue Engineering with Hyaluronic-Acid : Advances Application of Skin Regeneration Bone and Cartilage Repair

One method that has shown promise for healing damaged tissues is tissue engineering. The naturally occurring glycosaminoglycan hyaluronic acid (HA) has drawn interest because of its potential applications in the regeneration of skin, cartilage, and bone. The purpose of this study was to find out how well HA-based scaffolds work to encourage tissue repair and regeneration. We created and examined HA-based hydrogels, assessing their bioactivity, biocompatibility, and mechanical characteristics. Comparing HA-based scaffolds to controls, our findings showed increased tissue development, differentiation, and cell proliferation. HA-based constructions demonstrated noteworthy improvements in bone and cartilage regeneration in vivo, accompanied by notable increases in tissue integration and collagen deposition. These results demonstrate the potential of HA-based tissue engineering for the repair of bone, cartilage, and skin, providing a viable therapeutic approach for a range of dermatological and musculoskeletal applications.

FRESH 3D Bioprinting of Collagen Types I, II, and III.

Collagens play a vital role in the mechanical integrity of tissues as well as in physical and chemical signaling throughout the body. As such, collagens are widely used biomaterials in tissue engineering; however, most 3D fabrication methods use only collagen type I and are restricted to simple cast or molded geometries that are not representative of native tissue. Freeform reversible embedding of suspended hydrogel (FRESH) 3D bioprinting has emerged as a method to fabricate complex 3D scaffolds from collagen I but has yet to be leveraged for other collagen isoforms. Here, we developed collagen type II, collagen type III, and combination bioinks for FRESH 3D bioprinting of millimeter-sized scaffolds with micrometer scale features with fidelity comparable to scaffolds fabricated with the established collagen I bioink. At the microscale, single filament extrusions were similar across all collagen bioinks with a nominal diameter of ∼100 μm using a 34-gauge needle. Scaffolds as large as 10 × 10 × 2 mm were also fabricated and showed similar overall resolution and fidelity across collagen bioinks. Finally, cell adhesion and growth on the different collagen bioinks as either cast or FRESH 3D bioprinted scaffolds were compared and found to support similar growth behaviors. In total, our results expand the range of collagen isoform bioinks that can be 3D bioprinted and demonstrate that collagen types I, II, III, and combinations thereof can all be FRESH printed with high fidelity and comparable biological response. This serves to expand the toolkit for the fabrication of tailored collagen scaffolds that can better recapitulate the extracellular matrix properties of specific tissue types.

Alleviation of the degradation of cell wall pectin contributes to the maintenance of integrity of papaya (Carica papaya L.) fruit tissue during abnormal chilling injury behavior

Papaya (Carica papaya L.) is highly susceptible to chilling injury (CI), which primarily causes surface pitting, black scald, susceptibility to fungal infection, and the breakdown of tissue. It developed rapidly at 6 °C but was significantly suppressed at 1 °C, which indicated that the papaya tissue was more solid at a lower temperature. This rare phenomenon is considered to be abnormal CI behavior. The maintenance of fruit tissue integrity during abnormal CI behavior may be related to modifications of the cell wall pectin. The modifications of cell wall pectin were studied during storage at 1 and 6 °C. Storage at 1 °C alleviated the progression of CI and inhibited the degradation of pectin components and cell wall structure of papaya fruit. The increase in water-soluble pectin and the solubilization of cyclohexanediamine tetraacetic acid- (CDTA-) and sodium carbonate (Na2CO3)-soluble pectin was limited at 1 °C. Storage at this temperature limited the detachment of linear backbone chains and minimized the degradation of branching and reticular structure in the pectin molecules. Storage at this temperature also delayed the decrease in the size of pectin backbone and helped to prevent the loss of neutral sugars from the pectin side chains. Thus, alleviation of the degradation of cell wall pectin contributes to the structural integrity of the cell wall and thus helps the maintenance of the tissue integrity of papaya at 1 °C.

Dynamic conditioning of porcine kidney grafts with extracellular vesicles derived from urine progenitor cells: A proof-of-concept study.

: Among strategies to limit ischemia/reperfusion (IR) injuries in transplantation, cell therapy using stem cells to condition/repair transplanted organs appears promising. We hypothesized that using a cell therapy based on extracellular vesicles (EVs) derived from urine progenitor cells (UPCs) during hypothermic and normothermic machine perfusion can prevent IR-related kidney damage. We isolated and characterized porcine UPCs and their extracellular vesicles (EVs). Then these were used in an ex vivo porcine kidney preservation model. Kidneys were subjected to warm ischemia (32min) and then preserved by hypothermic machine perfusion (HMP) for 24h before 5h of normothermic machine perfusion (NMP). Three groups were performed (n=5-6): Group 1 (G1): HMP/vehicle + NMP/vehicle, Group 2 (G2): HMP/EVs + NMP/vehicle, Group 3 (G3): HMP/EVs + NMP/EVs. Porcine UPCs were successfully isolated from urine and fully characterized as well as their EVs which were found of expected size/phenotype. EVs injection during HMP alone, NMP alone, or both was feasible and safe and did not impact perfusion parameters. However, cell damage markers (LDH, ASAT) were decreased in G3 compared with G1, and G3 kidneys displayed a preserved tissue integrity with reduced tubular dilatation and inflammation notably. However, renal function indicators such as creatinine clearance measured for 5h of normothermic perfusion or NGAL perfusate's level were not modified by EVs injection. Regarding perfusate analysis, metabolomic analyses and cytokine quantification showed an immunomodulation signature in G3 compared with G1 and highlighted potential metabolic targets. In vitro, EVs as well as perfusates from G3 partially recovered endothelial cell metabolic activity after hypoxia. Finally, RNA-seq performed on kidney biopsies showed different profiles between G1 and G3 with regulation of potential IR targets of EVs therapy. We showed the feasibility/efficacy of UPC-EVs for hypothermic/normothermic kidney conditioning before transplantation, paving the way for combining machine perfusion with EVs-based cell therapy for organ conditioning. HIGHLIGHTS: ·UPCs from porcine urine can be used to generate a cell therapy product based on extracellular vesicles (pUPC-EVs). ·pUPC-EVs injection during HMP and NMP decreases cell damage markers and has an immunomodulatory effect. ·pUPC-EVs-treated kidneys have distinct biochemical, metabolic, and transcriptomic profiles highlighting targets of interest. ·Our results pave the way for combining machine perfusion with EV-based cell therapy for kidney conditioning.

The Management and Outcomes of Stoma Care with Impaired Integrity: A Case Study

Background: Colostomy globally affects not only physical but also psychological aspects in patients, and has been done for various reasons such as cancers of the colon and rectum. Tissue integrity disorders, irritations, and pain come up and require close service to care by nursing interventions and education for these conditions, especially in old patients. Purpose: The goal of this study was to evaluate the management and outcomes of stoma care in patients after colostomy surgery who were seen with tissue integrity disorders. Methods: The case-study approach was implemented into two male patients aged 76 and 80 years who were diagnosed with proximal rectal cancer and were given double-barreled colostomy treatment at Vila Alpina Hospital in São Paulo. Nursing intervention was on stoma care during cleaning and monitoring stoma characteristics as well as educating patients and their families on stoma care. Data were collected and analyzed over 3 days, while evaluation of outcome was done at tissue integrity and pain levels. Results: After the intervention of 3 days, partial improvement in tissue integrity was observed. The physical condition of both patients with normal tissue perfusion, absence of infection signs or bleeding, and partial developmental progress was seen in stoma wound healing. In addition, pain reduction was observed from a level of 4 to 2. However, healing was not fully achieved within the study period because of its short observation time. Conclusion: Nursing care considering observation, education, and therapeutic interventions will have improved outcomes of stoma and will also alleviate pain in colostomy patients. Further interventions of care and education to the patient will follow up these conditions for a long time.

ACCELERATION HEALING AND OSSEO-INTEGRATION PROCESSES OF CPTI IMPLANT BY COATING TEETH WITH COLLAGEN-POLYCAPROLACTONE FIBER SCAFFOLD

Objective: This study aimed to enhance osseointegration and healing by combining electrospinning techniques with collagen and polycaprolactone (PCL) as a coating for commercial pure titanium (CpTi) implants. Methods: In vitro experiments utilized glacial acetic acid as a solvent system to create collagen/PCL coatings with varying PCL concentrations (10%, 15%, 20% w/v). Morphological and physical characterizations were conducted using scanning electron microscopy (SEM), atomic force microscopy (AFM), and wettability tests. In vivo studies involved implanting collagen/PCL-coated CpTi cylinders into the femoral bone of New Zealand rabbits, followed by histological analysis at 2 and 6 weeks. Results: SEM revealed that scaffolds with higher PCL concentrations exhibited finer nanofiber structures (average diameter: 232 nm) and enhanced hydrophilicity and roughness. Histological analysis demonstrated significant osteogenic activity and basal bone formation, with well-formed bone plates observed at 6 weeks for implants coated with 20% collagen and 20% PCL. Novelty: The study highlights the potential of electrospun collagen/PCL coatings to create optimal surface properties for dental implants, achieving improved tissue integration and healing outcomes. This innovative approach demonstrates the versatility of electrospinning for fabricating fibrous scaffolds that can incorporate therapeutic agents, offering transformative implications for regenerative medicine and implantology.

Comparison of pathomorphologic properties of non-resorbable and partially resorbable implants for reconstructive surgery of pelvic organ prolapse and stress urinary incontinence: an animal model study

Introduction. It is well-known that the use of transvaginal mesh implants is not devoid of specific complications that meaningfully impair the quality of life of female patients. The volume of permanent material used has been shown to correlate closely with the risk of implant-associated complications. Thus, there is a need to develop a new generation of implants that retain the advantages of a permanent mesh implant but with minimal risk of implant-associated complications. Meanwhile, there are insufficient data on the organism's response to this type of synthetic implant.Objective. To compare the histological changes in the soft tissues of the rabbit anterior abdominal wall in the implantation zone of the new generation partially resorbable implant and non-absorbable polypropylene mesh implant on the animal model.Materials &amp; methods. We have previously developed a partially resorbable ligature delivery system for axial fixation of pelvic floor structures, performed technical analysis, and compared biomechanical and pathomorphological properties after implantation in an animal model experiment. The present study was conducted on 39 male rabbits of Chinchilla breed, randomised into three groups according to the type of implanted material: a partially resorbable ligature delivery system Axilen and Axilen Rapid [Lintex, LLC], and a non-resorbable polypropylene mesh implant Urosling. After the rabbits were removed from the experiment, the obtained biomaterial was subjected to histological study.Results. In the group with partially resorbable implants Axilen and Axilen Rapid, less inflammation and better tissue integration were observed compared to the polypropylene implant Urosling. This indicates a potential reduction in the risk of postoperative complications and an improvement in the quality of life for patients.Conclusion. The results of the experimental study confirm the advantages of using partially resorbable implants over traditional polypropylene meshes in pelvic floor reconstructive surgery. However, further research is needed to confirm the long-term safety and effectiveness of the new generation of implants.