Integrated Constructed Wetland Systems Research Articles

Organic media-based two-stage traditional and electrode-integrated tidal flow wetlands to treat landfill leachate: Influence of aeration strategy and plants

Landfill leachate treatment employing normal and electrode-integrated constructed wetlands is difficult due to the presence of significant amounts of organic compounds, which frequently impede the progression of microbial-based aerobic pollutant removal pathways. As a result, this study examines the effect of supplementary air availability via intermittent and continuous aeration strategies in improving organic, nutrient, and coliform removals of the unplanted, planted (normal and electrode-integrated) two-stage tidal flow constructed wetlands designed to treat landfill leachate. The constructed wetlands were filled with coal and biochar media and planted with Canna indica. Mean chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP), and coliform removal percentages of the externally aerated two-stage unplanted, only planted, planted-microbial fuel cell integrated constructed wetland systems ranged between 96 and 99%, 82 and 93%, 91 and 98%, 86 and 96%, respectively, throughout the experimental campaign. External aeration inhibited the development of a dominant anaerobic environment within the media of the wetland systems and improved overall pollutant removal. The electrode-integrated planted tidal flow wetlands produced better effluent quality than the unplanted or only planted tidal flow systems without electrode assistance. The first stages of the three wetland systems achieved an additional 5–7% COD, 7–12% TN, and 15–22% coliform removal during the continuous aeration period compared to the corresponding performance of the intermittent aeration phase. The pollutant removal performance of the second-stage wetlands decreased during the continuous aeration phase. The media composition supported electrochemically active and inactive microbial-based pollutant removal routes and the chemical adsorption of pollutants. Nitrogen and phosphorus accumulation percentage in plant tissues was low, i.e., 0.4–2.2% and 0.04–0.8%, respectively. During the continuous aeration period, the electrode-integrated tidal flow constructed wetlands achieved higher power density production, i.e., between 859 and 1432 mW (mW)/meter3(m3). This study demonstrates that external aeration might improve pollutant removal performance of the normal, electrodes integrated tidal flow-based constructed wetlands when employed for high organic-strength wastewater treatment such as landfill leachate.

Treating performance of a commercial-scale constructed wetland system for aquaculture effluents from intensive inland Micropterus salmoides farm

In intensive inland fish farming, discharge of untreated effluents adversely affects adjacent water bodies and causes water pollution. Thus, it is highly necessary to treat the effluents from inland fish farm. In this study, we built a commercial-scale integrated constructed wetland (CW) system with vertical subsurface flow, and monitored the purifying effect. During fish farming and discharge of effluents periods, the water samples were collected to detected the total nitrogen (TN), total phosphorus (TP), ammonia nitrogen (NH4+-N), nitrate nitrogen (NO3--N), nitrite nitrogen (NO2--N) and chemical oxygen demand (CODMn). Results showed that the system was stable and significantly improved water quality from fish pond. During the fish farming period, the removal efficiency for TN, TP, NH4+-N, NO3--N, NO2--N, and COD was 24.93–43.72%, 61.92–72.18%, 56.29–68.63%, 56.66–64.81%, 56.42–64.19% and 28.37–42.79%, respectively. Similarly, these parameters were also markedly decreased by the integrated CW system during sewage discharge period, and the average total removal rate for TN, TP, NH4+-N, NO3--N, NO2--N, and COD was 50.24%, 64.48%, 61.36%, 62.65%, 56.16% and 37.32%, respectively. It was worth noting that three key parameters for effluents detection TN, TP and COD values were below the threshold values of water quality of Class II in freshwater sewage discharge standard of China (SCT9101-2007). In conclusion, this study evidently demonstrated that application of CW system was an environmental sustainable sewage treatment strategy in intensive inland fish farming.

Critically important antimicrobial resistant Enterobacteriaceae in Irish farm effluent and their removal in integrated constructed wetlands

This study investigated the ability of Integrated Constructed Wetlands (ICWs) to remove critically important antimicrobial resistant organisms (AROs) from farm wastewater. Influent samples from the untreated farm waste and effluent samples taken at the end of the ICW system were collected monthly from four ICWs, serving four different farm types (suckler, dairy, dairy & poultry and pig). Using selective media to screen for the presence of carbapenemase resistant organisms, plasmid mediated and AmpC β-Lactamase producing organisms (ESBL/pAmpC) and fluoroquinolone resistant organisms, a total of 82 AROs were obtained with the majority being E. coli (n = 79). Statistically significant were the differences on the number of AROs isolated from influent (higher) compared to effluent, as well as a seasonal effect, with less AROs recovered during winter in comparison to other seasons (P < 0.05). On the other hand, there was no significant differences in the recovery of AROs on different farms. The majority of isolates from each of the farms (99%) were multi drug resistant, with 65% resistant to seven or more antimicrobials. A high incidence of tetracycline, trimethoprim/sulfamethoxazole, and ampicillin resistance was common to the isolates from all four farms but there were differences in ESBL levels with 63% of the isolates recovered from Farm 4 (piggery) being ESBLs compared to 18%, 36% and 4.5% recovered from Farms 1 (suckler), 2 (dairy) and 3 (dairy & poultry), respectively. No carbapenemase producing organisms were isolated. Our results showed that ICWs are effective in removing critically important AROs from farm wastewater on all four farm types.

Enhanced nitrogen removal by the integrated constructed wetlands with artificial aeration

Abstract The removal of organic pollutants, ammonia, and phosphorus from seriously polluted river water has been extensively explored using various traditional integrated constructed wetland systems. However, the removal efficiency of nitrogen was extremely low due to the limited oxygen concentration of these traditional systems. Herein, a novel pilot-scale integrated constructed wetland system (CWs), which consisted mainly of a biotic contacting oxidization pre-treatment (BCO), an artificial aeration vertical sub-surface flow CWs, and a horizontal subsurface flow CWs were applied to enhance the removal of total nitrogen (TN) under high hydraulic loading through artificial aeration. Results showed that, with the application of the novel CW systems, a total of 64.97% removal of TN was achieved with the average inflow of 11.39 mg/L and hydraulic load of 1.67 m3 ⋅ m−2 ⋅ d−1. Further analysis showed that the contributions of BCO, AVFCWs and HF CWs to TN removal were 13.86%, 15.97% and 35.36%, respectively. Besides the TN removal, the CW systems can also obtained about 75% of ammonia removal, within which artificial aeration vertical subsurface flow CWs unit occupied about 60% of contribution. Flowing by the application of this systems, the concentrations of ammonia and nitrate in the outflow decreased to 2.66 and 2.01 mg/L, respectively. The study indicated that the integrated constructed wetland system applied can be an efficiently potential technique for the removal of COD, NH4-N and TP from some rural wastewater.

Removal of steroid hormones and biocides from rural wastewater by an integrated constructed wetland

Steroid hormones and biocides are regarded as emerging contaminants in rural wastewater in China, owing to their widespread occurrence and adverse effects on both aquatic organisms and humans. Constructed wetlands (CWs) are an alternative technology for cost-effective and efficient decentralized rural sewage treatment. In this study, an integrated constructed wetland (ICW) system was built and used to treat a typical rural wastewater mixture composed of domestic sewage and livestock wastewater from a small village. As expected, five steroid hormones (ADD, AED, 19-NTD, T, and P) and four biocides (DEET, TCS, CBD, and MP) were detected in the influent in concentrations ranging from 30.5 ± 1.25 ng/L to 105 ± 5.14 ng/L and from 63.4 ± 2.85 ng/L to 515 ± 19.7 ng/L, respectively. The ICW system effectively removed the detected steroid hormones (97.4 ± 0.09%) and biocides (92.4 ± 0.54%). Based on the measured concentrations, the total pollution loadings of the detected steroid hormones and biocides in the influent were calculated to be 2330 ± 26.5 μg/day and 5710 ± 196 μg/day, which decreased to 60.8 ± 1.44 μg/day and 433 ± 25.6 μg/day in the final effluent. The risk quotients for these steroid hormones and biocides in the effluent from the ICW system were lower than those from reported wastewater treatment plants, indicating that CWs are a promising technology for removing contaminants including steroid hormones and biocides in rural wastewater, although additional efforts are required to optimize and improve the design of CWs before the steroid hormones and biocides present in the effluent can be safely and directly discharged into the environment.

Reactive carbon and nitrogen concentrations and dynamics in groundwater beneath an earthen-lined integrated constructed wetland

Using earthen liners at the base of an integrated constructed wetland (ICW) maintains a hydraulic continuum between the ICW and the underlying groundwater body. This may enable migration of reactive carbon (dissolved organic and inorganic carbon (DOC, DIC)) and reactive nitrogen (Nr) across this liner to groundwater along this continuum to groundwater. Therefore, in terms of a holistic assessment of ICWs, quantification of these losses is important and indeed from an environmental perspective comparing such losses to other ICW dis-connected areas within the same groundwater body is critical. The main objective of the present study on an ICW site in SE Ireland was to compare reactive forms of C and N in groundwater (three piezometer nests) representing the continuum end point of ICW Cell 2 and 3 with that of groundwater dis-connected to the ICW system. For this purpose two control planes consisting of shallow and deep groundwater piezometer nests were installed and monitored at 1 and 4 m depths, respectively underneath the earthen liners at 0.2 m adjacent to the ICW cells. Fieldwork was conducted between Nov 2013 and Nov 2014 and results showed that groundwater beneath the ICW liner had a highly reduced signal with C and N concentrations being significantly higher to those of the disconnected up-gradient groundwater. The DOC, DIC and dissolved CO2 and CH4 concentrations in groundwater beneath the ICW cells were significantly higher than the up-gradient groundwater. All the reactive forms of C were higher in Cell 2 than in Cell 3. With respect to depth, all forms of reactive C were higher in deeper groundwater than the shallow equivalents except for DOC. Equally, Nr concentrations (NH4+, DON, NO3−-N and N2O) were higher in groundwater beneath the ICW cells than the up-gradient groundwater. They were higher in Cell 2 than in Cell 3 and higher in shallow rather than deep groundwater. The fate of the reactive C and N is unknown. Typically DOC delivery from groundwater to surface water drives many biogeochemical processes as an electron donor to microbes or can be converted to CO2 and CH4 emissions and the dissolved CO2 and CH4 can be indirectly exchanged to the atmosphere. The results suggest that nutrient delivery from the ICW cells to the underlying groundwater needs to be evaluated and the design of earthen-lined ICWs should consider this nutrient loss continuum across the liner.

The biofilteration ability of oysters (Crassostrea gigas) to reduce Aeromonas salmonicida in salmon culture.

Pathogen contamination in the environment is inevitable with the rapid development of intensive aquaculture. Therefore, alternative ecofriendly biological strategies to control pathogenic bacteria are required. However, our aim was to investigate the ability of oysters (Crassostrea gigas) to filter the important opportunistic pathogen, Aeromonas salmonicida (strain C4), using a green fluorescent protein tag (GFP) in the Atlantic salmon (Salmo salar) farming wastewater. Hence, A. salmonicida removal efficiency and ingestion rate were detected in two different oyster stages (larvae and adults). To evaluate the practical performance of oysters as A. salmonicida biofilter, adult oysters were applied to an integrated constructed wetlands system (ICWS) and their long-term C4-GFP removal efficiency was recorded for 60days. Overall, our results clearly indicated that oysters had substantial A. salmonicida removal ability via their ingestion process when observed under a fluorescent microscope. Approximately 88-95% of C4-GFP was removed by oyster larvae at an ingestion rate of 6.4×103-6.2×105CFU/h·ind, while 79-92% of C4-GFP was removed by adult oysters at an ingestion rate of 2.1×104-3.1×106CFU/h·ind. Furthermore, 57.9±17.2% of C4-GFP removal efficiency was achieved when oysters were applied to ICWS. We, therefore, concluded that using oysters as a biofilter represents an effective alternative for removing A. salmonicida from aquaculture wastewater. However, the fate of oysters after ingesting the pathogenic bacteria, acting as a potential reservoir or vector for pathogens, is still debatable. This research provides the basis for the application of oysters as a biofilter to remove pathogens from aquaculture wastewater in industrialized production.

Correlating microbial community with physicochemical indices and structures of a full-scale integrated constructed wetland system

Microorganisms play a key role in removal of pollutants in constructed wetlands (CWs). The aim of this study was to investigate the composition and diversity of microbes in a full-scale integrated constructed wetland system and examine how microbial assemblages were shaped by the structures and physicochemical properties of the sediments. The microbial assemblages were determined using 16S rRNA high-throughput sequencing. Results showed that the microbial phenotypes were more diverse in the system than in single CWs. The genera of Zoogloea, Comamonas, Thiobacillus, Nitrosospira, Denitratisoma, Azonexus, and Azospira showed relatively high abundances, which contributed to the removal of organic matter and nitrogen. The interactions among the three CWs in series acted a key role in the increase of phylogenetic diversity and high percentage of shared operational taxonomic units. In the system, some core microbes always existed even with the changing environment. Redox potential and NH4-N were the important factors affecting the overall microbial community patterns. Total organic carbon had a relatively high impact on some denitrifiers. The results from this study should be useful to better understand the microbial mechanism of wastewater treatment in integrated constructed wetland systems.

Phosphorus retention and mass balance in an integrated constructed wetland treating domestic wastewater

This study quantified the contributions of different P removal pathways in an integrated constructed wetland (ICW) treating domestic wastewater. Findings over the study period (February 2008 to March 2012) showed average P retention rates of 31 ± 2 mg/m2/day for molybdate reactive phosphate (MRP) and 40 ± 3 mg/m2/day for total P. Near complete P removal was achieved during the first 2 years of operation. Thereafter, effluent concentrations increased slightly. According to the mass balance estimation, assimilation by plants accounted for approximately 16% of the total P retained, while sediment storage contributed nearly 60%. Sediment storage was the major P removal pathway in the ICW. Thus, high effluent concentrations recorded during high effluent flow volumes was due to remobilisation of P from the sediment. Management of ICW systems may therefore require implementing sediment removal schemes. The combination of plants with high biomass production can be beneficial for improving ICW performance.

Adaptive neuro-fuzzy inference system for real-time monitoring of integrated-constructed wetlands.

Monitoring large-scale treatment wetlands is costly and time-consuming, but required by regulators. Some analytical results are available only after 5 days or even longer. Thus, adaptive neuro-fuzzy inference system (ANFIS) models were developed to predict the effluent concentrations of 5-day biochemical oxygen demand (BOD5) and NH4-N from a full-scale integrated constructed wetland (ICW) treating domestic wastewater. The ANFIS models were developed and validated with a 4-year data set from the ICW system. Cost-effective, quicker and easier to measure variables were selected as the possible predictors based on their goodness of correlation with the outputs. A self-organizing neural network was applied to extract the most relevant input variables from all the possible input variables. Fuzzy subtractive clustering was used to identify the architecture of the ANFIS models and to optimize fuzzy rules, overall, improving the network performance. According to the findings, ANFIS could predict the effluent quality variation quite strongly. Effluent BOD5 and NH4-N concentrations were predicted relatively accurately by other effluent water quality parameters, which can be measured within a few hours. The simulated effluent BOD5 and NH4-N concentrations well fitted the measured concentrations, which was also supported by relatively low mean squared error. Thus, ANFIS can be useful for real-time monitoring and control of ICW systems.

Nitrogen transformations and mass balance in an integrated constructed wetland treating domestic wastewater.

Nitrogen (N) transformations and removal in integrated constructed wetlands (ICWs) are often high, but the contributions of various pathways, including nitrification/denitrification, assimilation by plants and sediment storage, remain unclear. This study quantified the contributions of different N removal pathways in a typical multi-celled ICW system treating domestic wastewater. Findings showed near complete average total N retention of circa 95% at 102.3 g m⁻² yr⁻¹ during the 4-year period of operation. Variations in total N and NH4-N removal rates were associated with effluent flow volume rates and seasons. According to the mass balance estimation, assimilation by plants and sediment/soil storage accounted for approximately 23% and 20%, respectively, of the total N load removal. These were the major N removal route besides microbial transformations. Thus, the combination of plants with high biomass production offer valuable opportunities for improving ICW performance. The retrieval and use of sequestered N in the ICW sediment/soils require coherent management and provide innovative and valuable opportunities.

Assessment of long-term phosphorus retention in an integrated constructed wetland treating domestic wastewater.

Due to the nature of the phosphorus (P) removal mechanisms associated with constructed wetlands, the sustainability of P treatment is usually of high interest. As a result, a 4-year dataset from a typical multi-celled integrated constructed wetland (ICW) located at Glaslough in Co. Monaghan, Ireland was evaluated to determine the effects of long-term P loadings and hydrological inputs on P treatment. The ICW was intensively monitored year-round from February 2008 through March 2012 for total P and molybdate reactive phosphate (MRP). Domestic wastewater was loaded at 16.4 ± 0.96 g m(2) year(-1) for total P and 11.2 ± 0.74 g m(2) year(-1) for MRP. Average mass reductions over the monitoring period were 91.4 and 90.1%, respectively. The area-based kinetic coefficients (K(20)) of 11.8 for total P and 15.6 m year(-1) for MRP indicated a high area-specific retention rate. The ICW appeared to have a sustained capacity for P adsorption and retention, but the treatment was influenced mainly by external hydrological inputs and fluctuations in wastewater loadings. Linear regression analyses showed a reduction in mass retention of both total P and MRP with increased effluent flow volumes. Monthly mass reductions exceeded 90% when the effluent flow volumes were less than 200 m(3) day(-1). When monthly effluent flow volumes exceeded 200 m(3) day(-1), nonetheless, mass reductions became highly variable. Designs and management of ICW systems should adopt measures to limit external hydrological loadings in order to maintain sufficient P treatment.

Groundwater Quality Impacts from a Full‐Scale Integrated Constructed Wetland

AbstractThe concept of integrated constructed wetlands (ICW) promotes in‐situ soils to construct and line wetland cells. The integrity of soil material, however, may provide a potential pathway for contaminants to flow into the underlying groundwater. This study assessed the extent of groundwater quality deterioration due to the establishment of a full‐scale ICW system treating domestic wastewater in Ireland. The ICW is located at Glaslough in Co. Monaghan, Ireland. It consists of two sedimentation ponds and a sequence of five shallow vegetated wetland cells. The ICW cells were lined with 500‐mm thick local subsoil material, which comprised a mixture of alluvium, organic soils, tills, and gravel. Groundwater samples and head data were collected from eight piezometers, which were installed around the ICW cells. The groundwater and wetland water samples were analysed for water quality parameters such as bulk organic matter, nutrients, and pathogens. Overall, the quality of groundwater underlying the ICW system recorded some contamination with bulk organic matter and some inorganic nutrients. Significantly higher contaminant concentrations were recorded in monitoring wells upgradient and near to the distal wetland cells than downgradient ones, which were near to the proximal cells. For the downgradient piezometers, concentrations seldomly exceeded the natural background levels. Detailed analyses through the application of chemometrics models indicated that the source of contamination was largely of geogenic origin. Findings suggest that ICW systems pose a minimal risk to the groundwater quality; the greatest risk was associated with the distal wetland cells.

Removal of antibiotics and antibiotic resistance genes in rural wastewater by an integrated constructed wetland

Integrated constructed wetlands (ICWs) are regarded as one of the most important removal technology for pollutants in rural domestic wastewaters. This study investigated the efficiency of an ICW consisting of a regulating pool, four surface and subsurface flow-constructed wetlands, and a stabilization unit for removing antibiotics and antibiotic resistance genes (ARGs) from rural domestic wastewaters. The results showed that antibiotics leucomycin, ofloxacin, lincomycin, and sulfamethazine, and ARGs sul1, sul2, tetM, and tetO were the predominant antibiotics and ARGs in the influent, respectively. The ICW system could significantly reduce most of the detected antibiotics and ARGs with their aqueous removal rates of 78 to 100 % and >99 %, respectively. Based on the measured concentrations, the total pollution loadings of antibiotics were 3,479 μg/day in the influent and 199 μg/day in the final effluent. Therefore, constructed wetlands could be a promising technology for rural wastewater in removing contaminants such as antibiotics and ARGs.

Integrated constructed wetlands: hotspots for freshwater coleopteran diversity in the landscape of Ireland

Constructed ponds are small water bodies that have an enormous biodiversity potential. The creation of this habitat can be seen as a valuable ecological enhancement tool, as ponds can host a great number of species. Integrated constructed wetlands (ICWs) are a type of surface-flow constructed wetland formed by interconnected ponds that integrate their water treatment capabilities with other functions such as biodiversity enhancement, carbon sequestration and landscape fit, by virtue of mimicking natural wetlands. ICWs have been shown to have the potential to enhance freshwater macroinvertebrate diversity in Ireland, especially Coleoptera diversity, in agricultural areas due to their unique design adaptability. A total of 67 water beetle taxa were found to inhabit the 15 studied ponds in the Annestown River catchment, Co. Waterford. Furthermore, when the taxa that have the potential to inhabit ICW ponds in Ireland were included a total of 82 water beetle taxa was obtained, which represents 26% of the known Irish aquatic lentic coleopteran fauna and includes several species of conservation interest. If the potential of these small water bodies to support water beetle diversity and that of other aquatic macroinvertebrates is to be maximised, particular attention should be paid to enhancing the range of mesohabitats within the ICW system.

Statistical Modeling of Contaminants Removal in Mature Integrated Constructed Wetland Sediments

Multiple regression models, principal component analysis, redundancy analysis, and the self-organizing map (SOM) model were applied to assess the effects of physico-chemical parameters on the treatment performance of contaminated wetland sediments in replicate integrated constructed wetland (ICW) mesocosms treating either farmyard runoff or domestic wastewater. Dissolved oxygen concentration and conductivity were correlated with the reduction of ammonia–nitrogen (NH4–N) and/or molybdate reactive phosphorus (MRP), and the redox potential and conductivity were related to chemical oxygen demand (COD) removal. SOM was selected as the prediction model to provide numerical estimations for the performance of ICW mesocosms. The model was validated, indicating that NH4–N, MRP, and COD removal can be predicted by input variables that are quick and cost-effective to measure. The SOM model is an appropriate method for monitoring the performance of mature ICWs as a source of contaminants such as nitrogen and phosphorus. Although no reduction in the overall performance was observed for ICW Sites 7 and 11, this laboratory-scale study provides valuable warning signs regarding the loss of contaminant removal for ICW systems that have been in operation for a relatively long time.

Characterising infiltration and contaminant migration beneath earthen-lined integrated constructed wetlands

The concept of integrated constructed wetlands (ICW) uses in situ soils to construct and line their cells. The integrity of soil materials, however, may provide a potential pathway for contaminants to flow into the subsoil. In this study, the rates of infiltration and contaminants loading occurring beneath a full-scale ICW treating domestic wastewater were evaluated over an 18-month period. The ICW is located at Glaslough in Co. Monaghan, Ireland. It consists of two sludge cells and a sequence of five shallow vegetated wetland cells. The ICW cells were lined with 500mm thick local subsoil materials. Infiltration water was collected from zero-tension pan lysimeters, which were placed within the soil-liners of the first three ICW cells and analysed for water quality parameters such as chemical oxygen demand, ammonia-nitrogen, nitrate-nitrogen, and molybdate reactive phosphate. Infiltration rates were 4.3×10−9, 3.7×10−9 and 1.0×10−8ms−1 from the first three ICW cells. The variation among locations was small. The apparent hydraulic conductivity of the soil liner, calculated with empirical water budgets, ranged between 3.5×10−9 and 9.8×10−9ms−1across the cells. In the first two cells of the ICW, less than 0.5% of the influent contaminant loading to the cells was lost through infiltration to subsoil. Overall, the amount of infiltration and contaminant loading occurring beneath the ICW cells increased from the proximal cells to the distal one. Higher contaminant loading was recorded in the third cell, which received partially treated wastewater. This implies that each ICW cell has the potential to impact differently on the underlying groundwater and that the potential for groundwater contamination from ICW systems was minimal in the first ICW cells, when compared to the last one.

A review of the potential of surface flow constructed wetlands to enhance macroinvertebrate diversity in agricultural landscapes with particular reference to Integrated Constructed Wetlands (ICWs)

Integrated Constructed Wetlands (ICWs) constitute an alternative way of cleansing water in agricultural areas to conventional water treatment plants. These pond systems integrate their water treatment capabilities with other functions such as biodiversity enhancement, carbon sequestration and landscape fit, by virtue of mimicking natural wetlands. ICWs have shown to have potential to enhance macroinvertebrate diversity in agricultural areas due to their unique design flexibility. We suggest that ICWs characterised by adequate shore sloping and presence of complex habitat mosaics, as well as other design considerations, would maximise the potential of these systems to enhance macroinvertebrate diversity. In addition, it would be highly beneficial to increase the total ICW area and to increase the total number of ponds in a system accordingly from a minimum of four to at least five. This article also highlights the need not only to develop policies that focus on the creation of ICWs but also to foster a number of management practices that rely on the active involvement of farmers. The inclusion of additional ponds in selected ICW systems could be the means to inform local communities as to the importance, attractiveness and conservation value of ponds.

Removal of Salmonella and indicator micro-organisms in integrated constructed wetlands treating agricultural wastewater

The purpose of this study was to investigate the removal of pathogenic and indicator micro-organisms in integrated constructed wetland (ICW) systems treating agricultural wastewater. Nine ICW's treating piggery (3) or dairy (6) wastewaters were sampled and indicator micro-organisms were enumerated in the influent as well as the effluent from the first, mid- and final cells. The presence/absence of Salmonella was also determined and any Salmonella isolates recovered were characterized. Mean counts of coliform, E. coli and Enterococcus across all nine ICW systems were lower in the final effluent than in the effluent from cell 1 (P < 0.001). An antibiotic susceptible isolate of Salmonella Dublin, a bovine-adapted serotype, was isolated from the influent to one dairy ICW but was not detected in any of the ICW cells. An antibiotic sensitive Salmonella Dublin isolate with the same molecular fingerprint was also recovered from the cell 1 effluent of another dairy ICW but was absent from the influent and the mid-cell and final effluents. Salmonella Typhimurium DT104b was detected in the liquid fraction of anaerobically digested pig manure as well as in the effluent from the first cell and mid-cell of an ICW treating this material, but was absent in the final effluent. Molecular fingerprinting by pulsed field gel electrophoresis demonstrated that the recovered isolates were highly related. However, they had different antimicrobial resistance profiles, with some highly resistant isolates recovered. In conclusion, counts of indicator micro-organisms were reduced significantly within ICW, with E. coli and Enterococcus non-detectable in the final effluent. Moreover, Salmonella, when present in the influent, appears to have been removed.

Meso-scale systems used for the examination of different integrated constructed wetland operations

Meso-scale constructed wetlands have not been commonly used for the examination of interactions and operations within differently designed wetland systems. Sixteen meso-scale constructed wetland systems (4 operations with 4 replicates each) were therefore evaluated between November 2008 and June 2010. These systems were used to examine key operations identified in the literature including hydraulic loading rates, nutrient loading rates and nutrient recycling modes. The wetlands were managed in the following modes: normal, recycling, high nutrient loading and high flow rate. The designs were such that the influents could be varied at set loading rates and concentrations. The ammonia removal rates for the meso-scale integrated constructed wetland (ICW) systems were similar to other full-scale ICW systems that have been in operation in the south-east of Ireland for the past decade. These comparable results highlight the potential cost-effectiveness of these meso-scale experimental designs for the examination of novel operations for future ICW developments.