This research explores a novel method for synthesizing Cr-Cu-C ternary carbide using a sol-gel technique. The study targeted to produce a wide range of transition metal carbides within the Cr-Cu-C system by varying the elemental ratios. The primary goal was to observe how compositional changes affect the morphological, structural, and chemical properties of the resulting ternary compounds resembling the MAX phase. Another focus is to establish a practical synthesis route and evaluating the materials' suitability for different applications. The synthesis process involved combining metal nitrates and citric acid, followed by carbothermal reduction. Comprehensive characterization techniques, including FTIR, XRD, SEM, EDX, XPS, and Raman spectroscopy, were employed to thoroughly recognize the prepared materials. In XRD, the calculated lattice parameters indicated hexagonal structures for all the prepared ratios, confirming successful synthesis and providing insights into their morphological and chemical properties. This has also been supported with other characterization techniques, Additionally, the Cr-Cu-C materials demonstrated strong antifungal and antibacterial effects against Candida albicans, E. coli, and B. cereus, highlighting their potential for biomedical applications. Furthermore, their anti-cancer potential against HepG2 liver cancer cells was assessed, showing effectiveness and inhibition among the samples. These findings highlight the potential of the synthesized Cr-Cu-C materials in diverse biomedical applications, supported by their structural integrity, morphological features, and antimicrobial properties.