Total Carotenoid Intake Research Articles

Skin Carotenoids Are Related to Cognitive Abilities among Toddlers

BackgroundCarotenoids are antioxidant pigments that deposit in human tissues (e.g., skin, macula, and brain) upon dietary consumption. The health implications of skin carotenoids, which reflect overall dietary carotenoid consumption, have not been adequately studied in younger populations. ObjectivesThis work aimed to examine links between skin carotenoids and cognitive, language, and motor skills among toddlers. A secondary aim explored relationships between skin carotenoids and neurophysiologic outcomes of sensory memory [i.e., mismatch negativity (MMN)] and the functional integrity of the visual pathway [i.e., visual evoked potentials (VEPs)]. MethodsToddlers aged 12–18 mo (n = 45) were included in a cross-sectional study. Skin carotenoids were measured with reflection spectroscopy via the Veggie Meter®. Cognitive, language, and motor skills were measured using the Bayley Scale of Infant and Toddler Development IV Screening Test (BSID-IV). MMN and VEPs were collected with an auditory oddball task and a pattern reversal task, respectively, using electroencephalography. Analyses adjusted for age, household income, highest level of parental education, and total carotenoid intake (mg/1000 kcal). ResultsRegression modeling revealed that skin carotenoids significantly related to cognition (β = 0.24; P = 0.04) and not to any other BSID-IV subsets. Neither MMN nor VEP outcomes significantly related to skin carotenoids. ConclusionsGreater skin carotenoids were selectively related to cognition, indicating that carotenoids may play a role in cognition in toddlers. Additional research is needed to understand links between skin carotenoids and specific domains of cognitive function and brain health in early life.

Association between dietary carotenoid intakes and the risk of asthma in children and adolescents: evidence from the National Health and Nutrition Examination Survey 2007-2016.

In recent years, the incidence of asthma in children has been increasing. As a chronic disease, in addition to drug treatment, dietary management is also important. However, studies of carotenoids and asthma have shown mixed results. This study aimed to evaluate whether the relationship between carotenoid intake and current asthma holds significant importance. We studied 9,118 children aged 6-16 years in the National Health and Nutrition Examination Survey (NHANES) of US from 2007 to 2016, and the relationship of dietary carotenoid and its subgroup with pediatric asthma. Current asthma was assessed by parent-reported, doctor-diagnosed, asthma using a standardized questionnaire. We used multivariate logistic regression to calculate the odds ratio (OR) for current asthma with a 95% confidence interval (CI). Total carotenoid intake was not associated with the risk of current asthma. Compared with the first quantile, the second quantile of β-cryptoxanthin intake was positively correlated with current asthma (Q2: 1.227; 95% CI: 1.025-1.470; P=0.03). The test of trend showed that, as the α-carotene intake increased, the risk of current asthma showed a decreasing trend, which was very close to the statistic confidence cutoff (Model I: P for trend =0.001; Model II: P for trend =0.003; Model III: P for trend =0.08). In subgroup analysis, family history of asthma interacted with carotenoid intake (P=0.005). The population without a family history of asthma, there were significant negative associations between carotenoid intakes and asthma (quartile 4: Model III: 0.720; 95% CI: 0.549-0.943; P=0.02). In this study, pediatric current asthma was not related to total carotenoids in our total participants. Total dietary carotenoid intake has a protective effect on children without a family history of asthma. Meanwhile, β-cryptoxanthin intake is positively correlated with asthma.

Association of Dietary Carotenoids Intakes with Obesity in Adults: NHANES 2007-2018.

Previous studies on the relationship between dietary carotenoids and obesity are few, and the results are inconsistent. Therefore, the purpose of this study was to evaluate the associations between total carotenoids, α-carotene, β-carotene, β-cryptoxanthin, lycopene, and lutein+zeaxanthin intakes and the risk of obesity in adults. This cross-sectional study was carried out based on the National Health and Nutrition Examination Survey (NHANES) 2007-2018. The two 24-h dietary recall interviews were used to collect dietary carotenoids data. Obesity was defined as body mass index (BMI) ≥30 kg/m2. Logistic regression models and restricted cubic spline were used to assess the relationships between carotenoids intakes and obesity. A total of 25,868 adults aged 20 and over were enrolled in the present study. In multivariate logistic regression model 2, the odds ratios (ORs) with 95% confidence intervals (CIs) of obesity were 0.82 (0.74-0.91), 0.80 (0.72-0.90), 0.77 (0.69-0.87), 0.85 (0.76-0.95), and 0.80 (0.73-0.89) for the highest versus the lowest of total carotenoids, α-carotene, β-carotene, β-cryptoxanthin, and lutein+zeaxanthin intakes, respectively. Dose-response analyses suggested that the carotenoids mentioned above were inversely related to obesity in a nonlinear manner. In multivariate-adjusted model 2, total carotenoids intake were associated with lower risk of Class 1, 2, and 3 obesity. This study indicated that intakes of total carotenoids, α-carotene, β-carotene, β-cryptoxanthin, and lutein+zeaxanthin were inversely associated with the risk of obesity.

Examination of the utility of skin carotenoid status in estimating dietary intakes of carotenoids and fruits and vegetables: A randomized, parallel-group, controlled feeding trial

ObjectiveOptical spectroscopy-measured skin carotenoid status (SCS) has been validated for estimating fruit and vegetable (F&V) intake; however, there is limited research addressing SCS kinetics in whole-diet interventions. The aim of this controlled feeding trial was to explore SCS's response to carotenoid intake changes via whole-diet intervention, evaluating its biomarker potential. MethodsEighty participants ages 20 to 49 y, without underlying diseases, were randomly allocated to the high-carotenoid group (HG; n = 40) or control group (CG; n = 40). The HG consumed a high-carotenoid diet (21 mg total carotenoids/2000 kcal), whereas the CG consumed a control diet (13.6 mg total carotenoids/2000 kcal) for 6 wk. Subsequently, skin and blood carotenoid concentrations were tracked without intervention for 4 wk. SCS was measured weekly via resonance Raman spectroscopy, and serum carotenoid concentrations were analyzed biweekly using high-performance liquid chromatography. Baseline carotenoid and F&V intakes were assessed via a 3-d diet record. The kinetics of SCS and serum carotenoid concentrations were analyzed using a weighted generalized estimating equation. Pearson's correlation analyses were used to examine baseline correlations between SCS and dietary carotenoid and F&V intakes, as well as serum carotenoid concentrations. ResultsDuring the intervention, the HG showed a faster and greater SCS increase than the CG (difference in slope per week = 8.87 AU, Pinteraction <0.001). Baseline SCS had positive correlations with total carotenoid intake (r = 0.45), total F&V intake (r = 0.49), and total serum carotenoid concentration (r = 0.79; P < 0.001 for all). ConclusionThese results suggest that SCS is a valid biomarker for monitoring changes in carotenoid intake through whole diet, which supports using SCS for assessing carotenoid-rich F&V intake.

Aging and antioxidants: the impact of dietary carotenoid intakes on soluble klotho levels in aged adults.

The association between dietary carotenoid intake and Soluble Klotho (S-Klotho) levels among the elderly population requires further evaluation. The purpose of this study is to evaluate the relationship between the dietary carotenoid intake and the S-Klotho plasma levels in older adults. Eligible participants aged 60 years or above were selected from the National Health and Nutrition Examination Surveys (NHANES) data, collected between 2007 and 2016. The consumption of carotenoids was determined through two 24-hour dietary recall assessments. Moreover, the S-Klotho levels in the serum were measured using an Enzyme-Linked Immuno-Sorbent Assay (ELISA). A total of 5,056 participants were included in the study having a median total carotenoid intake of 9775.25 μg (95% confidence interval (CI): 8971.30-10579.21) and a median S-Klotho concentration of 815.59 pg/mL (95% CI: 802.59-828.60). The multivariable regression analysis showed that a single standard deviation increase in total carotenoid intake was significantly associated with an 8.40 pg/mL increase in S-Klotho levels (95% CI: 0.48-16.31). When the carotenoids were divided into quartiles, participants in the third ((4963.5μg/day,11662.5μg/day]) and fourth quartiles ((11662.5μg/day,377178μg/day]) showed higher S-Klotho levels compared to those in the first quartile. Among carotenoid subtypes, increased intake of α-carotene, β-carotene, and lutein with zeaxanthin was associated with elevated S-Klotho levels. These observed associations between carotenoid subtypes and S-Klotho levels remained consistent across male participants, having a normal weight, and a moderate physical activity based on stratified analysis. The total carotenoid intake was positively related to plasma levels of S-Klotho in the elderly population, particularly for α-carotene, β-carotene, and lutein with zeaxanthin. However, further research is needed to confirm these findings and explore the underlying mechanisms behind this relationship.

Skin Carotenoids Measured by Reflection Spectroscopy Correlate With Dietary Carotenoid Intake in Racially and Ethnically Diverse US Toddlers From Houston, Texas

BackgroundMethods to objectively assess fruit and vegetable (FV) intake in young children are needed in order to support rigorous assessments of policies and interventions. Noninvasive skin carotenoid concentration measurements may provide a rapid assessment of toddler carotenoid and carotenoid-rich FV intake. ObjectiveThe aim of this study was to evaluate the relationship between pressure-mediated reflection spectroscopy-measured skin carotenoid score (SCS) with proxy-reported carotenoid and FV intake in racially and ethnically diverse US toddlers. DesignThis study was a secondary analysis of data obtained from a randomized controlled 10-week study of the effect of an interactive healthy playgroup intervention vs classroom parent education on the diet and physical activity of toddlers. This study collected skin carotenoid and 1-week dietary intake using a 31-item semi-quantitative food frequency questionnaire analyzed by the Nutrient Data System for Research nutrient database. The current study determined dietary predictors of toddler SCS. Participants/settingParticipants were racially and ethnically diverse US toddlers (12 to 36 months) and adult guardian dyads (n = 50) recruited from the community in Houston, TX from fall 2018 to spring 2019. Main outcome measuresAt baseline and 10 to 12 weeks after baseline, SCS was measured by means of pressure-mediated reflection spectroscopy (Veggie Meter, Longevity Link Corporation), guardians reported toddler diet using a food frequency questionnaire, and carotenoid intake was estimated from the food frequency questionnaire responses using a nutrient database. Statistical analysisThe relationship between toddler SCS and intake of total and individual carotenoid species and FV servings was tested using generalized linear mixed models, controlling for body mass index-for-age percentiles, group assignment, and age. ResultsSCS was positively and significantly predicted by estimated intakes of total carotenoids (P = .002), β-carotene (P ≤ .001), and lutein and zeaxanthin (P = .003). Reported intakes of α-carotene, β-cryptoxanthin, and lycopene were not predictors of SCS. SCS was predicted by estimated total FV intake (P = .047) and vegetable intake (P = .006), but not fruit intake (P = .580). ConclusionsThese results showed that reported dietary carotenoid intake is a significant predictor of SCS in an ethnically and racially diverse population of US toddlers. Toddler skin carotenoid measurement holds promise as a rapid, objective, noninvasive biomarker of dietary carotenoid intake.

Assessment of dietary carotenoid intake and food sources in the Brazilian population: Results from National Dietary Surveys 2008–2009 and 2017–2018

There is an inverse association between carotenoids intake and disease risk. However, the lack of population-level intakes difficult research on this topic. We aimed to assess the intake and major food sources of carotenoids in Brazil, as well to characterize the intake changes according to demographic characteristics. Data from two cross-sectional population-based surveys were analyzed in the study (National Dietary Survey 2008–2009 and 2017–2018). Carotenoid content of foods was identified using data from obtained by means of high-performance liquid chromatography. The median (25th-75th percentiles) of energy-adjusted total carotenoid intake in 2017–2018 was 12.2 (6.0–22.6) mg/ 1000 kcal/ d, representing an increase of 3.7 mg/d from 2008 to 2009. The carotenoids most consumed were β-carotene, lutein, and lycopene, which showed an increase in consumption in the period. The total carotenoid intake and the majority of classes were higher by females, whites, adults and elderly, and those living in urban areas (p < 0.0001). The main food sources of carotenoids intake presented some variations between 2008 and 2009 and 2017–2018, with greater contribution of salads, and decrease of pumpkin, kale, and orange contribution. Our results support further studies investigating temporal trends in carotenoids intake and disease risk, being useful for the establishment of adequate reference intake recommendations.

Bioactive Compounds Intake of the Brazilian Population According to Geographic Region.

Studies have been conducted in order to estimate bioactive compound consumption across populations, with substantial disparities according to the origin of the cohort examined. In this sense, Brazil is a continental country with marked differences in food plant availability across geographic regions. We aimed to estimate the bioactive compound intake according to Brazilian geographic region, as well as to determine the major contributors. Data were obtained from the National Dietary Survey 2017-2018, a cross-sectional population-based study including data on the individual food intake of 46,164 subjects aged ≥10 years. The consumption of polyphenols (total and classes) was significantly higher in the South compared with other regions (p = 0.0001). Total carotenoid intake was higher in the Midwest, followed by the Southeast (p = 0.0001). Tea was the main supplier of total polyphenol intake in the South, whereas coffee contributed the most to total polyphenol intake in other Brazilian regions. Açaí, caja juice, mango and corn were important suppliers of carotenoid intake in the North and Northeast. Bioactive compound intake presented variations according to Brazilian region, and individuals living in the South, Midwest and Southeast may experience higher bioactive-dense diets. We highlight the potential of many food plants for sustained explorations to the development of marketable products, possibly increasing the bioactive compound intake.

Validation of resonance Raman spectroscopy-measured skin carotenoid status as a biomarker for fruit and vegetable intake in Korean adults.

Blood carotenoid concentration measurement is considered the gold standard for fruit and vegetable (F&V) intake estimation; however, this method is invasive and expensive. Recently, skin carotenoid status (SCS) measured by optical sensors has been evaluated as a promising parameter for F&V intake estimation. In this cross-sectional study, we aimed to validate the utility of resonance Raman spectroscopy (RRS)-assessed SCS as a biomarker of F&V intake in Korean adults. We used data from 108 participants aged 20-69 years who completed SCS measurements, blood collection and 3-d dietary recordings. Serum carotenoid concentrations were quantified using HPLC, and dietary carotenoid and F&V intakes were estimated via 3-d dietary records using a carotenoid database for common Korean foods. The correlations of the SCS with serum carotenoid concentrations, dietary carotenoid intake and F&V intake were examined to assess SCS validity. SCS was positively correlated with total serum carotenoid concentration (r = 0·52, 95 % CI = 0·36, 0·64, P < 0·001), serum β-carotene concentration (r = 0·60, 95 % CI = 0·47, 0·71, P < 0·001), total carotenoid intake (r = 0·20, 95 % CI = 0·01, 0·37, P = 0·04), β-carotene intake (r = 0·30, 95 % CI = 0·11, 0·46, P = 0·002) and F&V intake (r = 0·40, 95 % CI = 0·23, 0·55, P < 0·001). These results suggest that SCS can be a valid biomarker of F&V intake in Korean adults.

Carotenoids: dietary sources, adequate and clinically effective doses

Natural food sources of carotenoids are colored vegetables and fruits, eggs, milk. Total carotenoid intake varies from 1 to 22 mg/day in European countries and from 5 to 16 mg/day in the USA. The average daily intake of lycopene in Europe ranges from 5 to 7 mg/day, in the USA – from 5.7 to 10.5 mg/day. Carotenoids are used in the production of fortified foods, biologically active food supplements, specialized foods for various population groups, as well as natural food colors added directly during the production of a food product or in feed for farm animals. Epidemiological studies have shown that dietary carotenoid intake is inversely associated with obesity, retinopathy, cataracts; cardiovascular disease (CVD), diabetes and some cancers. According to the domestic regulatory framework, the adequate level of daily intake of carotenoids is 15 mg, 5 mg of β-carotene, lycopene and lutein, the upper permissible level of consumption of carotenoids as part of dietary supplements and specialized food products is 30 mg (10 mg each). For zeaxanthin, these values are 1 and 3 mg/day. Daily doses of lycopene used in intervention studies were 15–90 mg, and β-carotene 15–50 mg. lutein – 4–20 mg; zeaxanthin – 1–20 mg; mezoxanthin 12 mg, astaxanthin – 12–16 mg. Duration of admission was 4–12 months (up to 48 months). Taking lycopene at a dose of 10–15 mg for several months led to a decrease in indicators of oxidative stress, markers of bone resorption, taking lutein at a dose of ≥ 10 mg improved visual and cognitive functions. The effective doses of carotenoids correspond to or exceed the permitted allowable level of their consumption as part of dietary supplements and specialized food products.

Biochemical Validation of a Self-Administered Carotenoid Intake Screener to Assess Carotenoid Intake in Nonobese Adults

BackgroundEpidemiological studies have demonstrated an association between carotenoid intake and health. However, an accurate measurement of carotenoid intake is challenging. FFQ is the most commonly used dietary assessment method and is typically composed of 100–200 items. However, the greater participant burden that accompanies a more detailed FFQ provides only a marginal gain in accuracy. Therefore, a brief validated carotenoid intake screener is needed. ObjectivesTo conduct secondary analysis evaluating the validity of a newly developed 44-item carotenoid intake screener from The Juice Study: Sensitivity of Skin Carotenoid Status to Detect Change in Intake (NCT03202043) against corresponding plasma carotenoid concentrations (primary) and skin carotenoids (secondary) in nonobese Midwestern American adults. MethodsHealthy adults (n = 83; 25 men and 58 women) aged 18–65 y (mean age, 32 ± 12 y) with a BMI (in kg/m2) of 18.5–29.9 (mean BMI, 25 ± 3) were recruited between 25 April 2018 and 28 March 2019. Participants completed the carotenoid intake screener weekly during the 8-wk parent study. Plasma carotenoid concentrations were assessed at weeks 0, 4, and 8 using HPLC. Skin carotenoids were assessed weekly using pressure-mediated reflection spectroscopy (RS). Correlation matrices from mixed models were used to determine the correlation between carotenoid intake and plasma and skin carotenoids over time. ResultsThe total carotenoid intake, as determined by the carotenoid intake screener, correlated with both the plasma total carotenoid concentration (r = 0.52; P < 0.0001) and the RS-assessed skin carotenoid concentration (r = 0.43; P < 0.0001). Correlations between reported intake and plasma concentrations of α-carotene (r = 0.40; P = 0.0002), cryptoxanthin (r = 0.28; P = 0.0113), and lycopene (r = 0.33; P = 0.0022) were also observed. ConclusionsThe results of this study demonstrate an acceptable relative validity of the carotenoid intake screener to assess total carotenoid intake in adults classified as those having a healthy body or those with overweight.

Noninvasive Reflection Spectroscopy Measurement of Skin Carotenoid Score in Infants Is Feasible and Reliable

Skin carotenoid measurement by reflection spectroscopy (RS) offers a noninvasive biomarker of carotenoid intake, but feasibility, reliability, and validity are not established in infants. In this study we aimed to determine the feasibility and reliability of 4-mo-old infant skin carotenoid score (SCS) measurement and its correlation with total carotenoid intake and plasma concentrations. SCSs were measured in a prospective, observational study with a modified, portable RS device at the index finger and heel of the foot in 4-mo-olds (n = 21). Infant plasma, human milk, and formula carotenoid concentrations were measured by HPLC-photodiode array, and carotenoid intake was estimated from 7-d food diaries corrected for actual milk carotenoid content. Mean SCS, time to acquire measurements, replicate intraclass correlations, and bivariate correlations between SCS, carotenoid intake, and plasma carotenoids were examined. Exploratory analyses of returning 6- (n = 12) and 8-mo-old (n = 9) infants were conducted. Mean ± SD finger and heel SCSs in 4-, 6-, and 8-mo-olds were 92 ± 57 and 92 ± 51; 109 ± 41 and 119 ± 44; and 161 ± 89 and 197 ± 128 units, respectively. Replicate SCS measurements were reliable, with high intraclass correlation (≥0.70) of within-subject visit measurements. Finger SCSs in 4-mo-olds were correlated with carotenoid intake (ρ = 0.48, P = 0.0033), and finger and heel SCS were correlated with total plasma carotenoid concentrations (ρ = 0.71, P < 0.0001 and ρ = 0.57, P = 0.0006, respectively). Eight-mo-olds' finger and heel SCSs were correlated with total carotenoid intake (ρ = 0.73, P < 0.001; ρ = 0.58, P = 0.0014, respectively), whereas SCSs in 6-mo-olds, in transition from exclusive milk to complementary feeding, did not correlate with plasma carotenoid or dietary carotenoids, despite correlation between plasma and dietary carotenoid intake (ρ = 0.86, P = 0.0137). Mixed models suggest plasma total carotenoid concentration, age, carotenoid intake, and age × carotenoid intake, but not measurement site, are determinants of infant SCS. Infant skin carotenoids are feasibly and reliably measured by RS and may provide a biomarker of carotenoid intake in 4-mo-olds. This trial was registered at clinicaltrials.gov as NCT03996395.

Adherence to the Mediterranean-style diet and high intake of total carotenoids reduces the odds of frailty over 11 years in older adults: Results from the Framingham Offspring Study

The benefit of a Mediterranean-style diet in reducing frailty is not well established in older Americans. We sought to determine associations of a Mediterranean-style dietary pattern and related antioxidants with frailty onset and worsening of the Fried phenotype in adults. This prospective study included 2384 nonfrail adults from the Framingham Offspring Study with a Mediterranean-style dietary pattern score (MSDPS) and data on antioxidant intakes (vitamin C, E, and total carotenoids) estimated from an FFQ at the index examination (1998-2001) and 1 prior examination (if available), as well as a frailty assessment at the index examination and at least 1 follow-up. Frailty onset was defined as ≥3 of 5 Fried frailty phenotype criteria at follow-up and the worsening of the Fried frailty phenotype was defined as an increased number of frailty criteria over follow-up (yes or no). Logistic regression with generalized estimating equations estimated ORs and 95% CIs, adjusting for confounders. Analyses were stratified by age (<60 and ≥60 years) for significant interactions. The mean ± SD age was 60 ± 9 years (range, 33-86 years) and 55% were female. In adjusted models, a 1-unit higher MSDPS reduced the odds of frailty by 3% (OR, 0.97; 95% CI: 0.96-0.99). Each 10-mg higher total carotenoid and vitamin E intake reduced the odds of frailty by 16% (OR, 0.84; 95% CI: 0.73-0.98) and 1% (OR, 0.99; 95% CI: 0.98-1.00), respectively. No association with vitamin C (P = 0.36) was observed. The associations among participants aged <60 years of age were stronger for each 1-unit higher MSDPS (OR, 0.93; 95% CI: 0.89-0.96) and total carotenoid intake (OR, 0.59; 95% CI: 0.41-0.82) than those observed in older individuals [ORs, 0.98 (95% CI: 0.97-1.00) and 0.92 (95% CI: 0.79-1.08), respectively]. Our findings suggest that adherence to a Mediterranean-style diet and higher total carotenoid intake are associated with frailty prevention over time, particularly in adults <60 years.

Association between dietary carotenoid intakes and the risk of asthma in adults: a cross-sectional study of NHANES, 2007–2012

ObjectiveTo investigate the association between dietary carotenoid intake and asthma using data from a nationally representative sample of US adults.DesignCross-section study.SettingThe National Health and Nutrition Examination Survey 2007–2012.ParticipantsA total of...

Dietary carotenoid intake and risk of developing preeclampsia: a hospital-based case–control study

BackgroundThe effect of carotenoids on the risk of preeclampsia (PE) is uncertain. We aimed to examine the associations between the intake of dietary carotenoids and related compounds by pregnant women in China, and the risk of their developing PE.MethodsFour hundred and forty PE cases and 440 age- (± 3 years), gestational age- (± 1 weeks) and gestational diabetes mellitus status- (yes/no) matched healthy controls were recruited from March 2016 to June 2019. Dietary intake of carotenoids was assessed using a 79-item validated food-frequency questionnaire. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using conditional logistic regression.ResultsAfter adjusting for potential confounders, we found that the intake of total carotenoids, β-carotene, β-cryptoxanthin, lycopene, and lutein and zeaxanthin (lut-zea) were negatively associated with the odds of developing PE. Compared with the lowest quartile intake, the multivariate-adjusted OR (95% CI) of the highest quartile intake was 0.29 (0.16–0.54, Ptrend < 0.001) for total carotenoids, 0.31 (0.16–0.58, Ptrend < 0.001) for β-carotene, 0.50 (0.27–0.90, Ptrend = 0.007) for β-cryptoxanthin, 0.55 (0.30–0.99, Ptrend = 0.04) for lycopene and 0.32 (0.17–0.61, Ptrend = 0.001) for lut-zea. However, no significant associations were observed between the risk of developing PE and α-carotene intake (OR = 0.75, 95% CI: 0.41–1.36, Ptrend = 0.28). Moreover, similar negative associations were found for every one-standard-deviation increase in the intake of total carotenoids, β-carotene, β-cryptoxanthin, lycopene and lut-zea.ConclusionThese results indicate that a high intake of total carotenoids, β-carotene, β-cryptoxanthin, lycopene and lut-zea may be associated with a low risk of developing PE.

Dietary carotenoid intake and dental fluorosis in relation to SOD2 (rs 11968525) polymorphisms in Guizhou, China.

Genetic and dietary factors are important contributors to the development of dental fluorosis (DF). This study investigated the association between DF and dietary carotenoids, and explored whether the association was modified by polymorphisms of the antioxidant enzyme superoxide dismutase 2 (SOD2 rs11968525) in Guizhou, China. A cross-sectional study with a total of 899 adults aged 18-75 years were enrolled in the study. Face-to-face interviews were conducted to assess dietary habits using a validated 75 item food frequency questionnaire (FFQ). Sociodemographic and lifestyle information, and blood and urine samples were also collected. Genotypes were evaluated using TaqMan single nucleotide polymorphism (SNP) Genotyping Assay. There were significant dose-dependent inverse associations of the prevalence of DF with intake of α-carotene, β-carotene, lutein/zeaxanthin, lycopene and total carotenoids (p-trend ranged from <0.001-0.004). The odds ratios (ORs) and 95% confidence intervals (CIs) of DF comparing the highest against lowest quartile were 0.56 (0.35, 0.92) for α-carotene, 0.53 (0.35, 0.81) for β-carotene, 0.44 (0.27, 0.74) for lycopene, 0.35 (0.21, 0.58) for lutein/zeaxanthin in combination and 0.42 (0.25, 0.69) for total carotenoids (all p-trend<0.005). Intake of β-cryptoxanthin was not found to be related to DF. The inverse association of DF with dietary intake of α-carotene and β-carotene was more evident in individuals with the AG+AA genotype (p-interaction<0.05). Higher dietary carotenoids were associated with a lower occurrence of DF, polymorphisms in SOD2 (rs 11968525) modified the associations between dietary intake of carotene and DF. These findings provide evidence for precision prevention of fluorosis.

Assessment of dietary carotenoid intake and biologic measurement of exposure in humans.

Carotenoids are a diverse family of phytochemicals with over 1000 different carotenoids present in nature. A human diet containing a variety of plant foods typically includes approximately 50 different carotenoids, although six (α-carotene, β-carotene, β-cryptoxanthin, lycopene, lutein, and zeaxanthin) comprise over 90% of total carotenoid intake. Most carotenoids do not meet the definition of a nutrient, but several can be cleaved to form vitamin A and are important contributors to vitamin A nutriture and prevention of vitamin A deficiency. Large epidemiologic studies suggest that diets rich in total or specific carotenoids are associated with a reduced risk of several diseases including various types of cancer, cardiovascular disease, cognitive disorders, and age-related macular degeneration. However, accurate measurement of dietary intake is challenging and current methods of dietary assessment, including food frequency questionnaires, diet records and 24-h recalls, have strengths and limitations regarding estimating carotenoid intake. Additionally, carotenoid bioavailability from the diet is influenced by many variables including food processing and cooking, meal composition, and individual characteristics of the host including age, digestive efficiency, nutritional status and genetic polymorphisms. Carotenoids are deposited in many human tissues and can be measured using a variety of techniques including high performance liquid chromatography (HPLC) and mass spectrometry (MS). Continued research is necessary to improve dietary intake assessment and establish biologically relevant dose-response relationships in the context of individual variability to advance our understanding of diet, disease risk, and health promotion.

Assessment of dietary intake of bioactive food compounds according to income level in the Brazilian population.

There is an inverse association between bioactive compounds intake and disease risk. The knowledge of its consumption according to socio-economic strata is important, which allows identification of potential intervention targets. Thus, we aimed to investigate bioactive compounds intake according to income level in Brazilian population. Data were obtained from the Brazilian Household Budget Survey, a cross-sectional survey which included data on individual food intake of 34,003 subjects aged 10 years and over collected using two 24-h dietary records. Polyphenol and carotenoid content of foods was identified using published databases. Total polyphenol and carotenoid intake were determined according to per capita income, as well as main food sources. Total polyphenols and flavonoids intake increased with income level, and subjects with lower income showed higher phenolic acids intake than individuals in highest income (p = 0.0001). Total carotenoids and classes intake (with exception to β-cryptoxanthin and zeaxanthin) were higher among subjects in highest income quartile, compared to the lowest quartile (p = 0.0001). Coffee was major source to total polyphenols and phenolic acids intake, and orange juice was main flavonoid provider in individuals from all income levels. In the upper income quartile, total carotenoid was supplied mainly by tomato and kale, and fruits had important contribution to carotenoid intake in the lowest income quartile. There is important influence of income level on diet quality regarding intake of foods with bioactive compounds, and individuals with lower income may experience lower quality diets due to less availability of foods with bioactive compounds.

Antioxidants Such as Flavonoids and Carotenoids in the Diet of Bogor, Indonesia Residents

Due to the strong antioxidant activity of flavonoids and carotenoids, daily consumption of these bioactive compounds has the potential for reducing the risk of many chronic and degenerative diseases caused by or contributed to by oxidative stress. Currently, the available research results related to the flavonoid and carotenoid intake in Asian countries are very limited, especially for Indonesian population. The present study was conducted in Bogor City and Bogor District, West Java, Indonesia. Food consumption data was obtained through the food frequency questionnaire (FFQ) method, involving 200 respondents aged 25–65 years old. Flavonoids and carotenoids contents of the consumed food items were determined by referring to the databases developed by the United States Department of Agriculture (USDA), scientific journals, and calculation based on the recipes recorded in the survey. The total flavonoid intake of Bogor adults was estimated as 149.5 mg/day, consisting of 49.4% isoflavones, 24.0% flavonols, 9.4% flavanones, 7.0% flavan-3-ols, 6.0% flavones, and 4.2% anthocyanidins, and was contributed to mainly by legumes (70.7%), vegetables (10.1%), and fruits (7.3%). At the same time, the estimated total carotenoid intake reached 7.6 mg/day, and was contributed to mainly by vegetables (53.9%), fruits (20.2%), and snacks (14.4%), with β-carotene consumed in the highest proportion (49.9%), followed by lycopene (19.9%), lutein and zeaxanthin (13.5%), α-carotene (6.9%), and β-cryptoxanthin (2.6%). The effects of different respondents’ characteristics, such as area of residence (city vs. district), gender (male vs. female), and age (25–40, 41–55, and 56–65 years old) on the flavonoid and carotenoid intake varied widely, due to the differences in the overall consumption patterns of the respective respondents’ groups.

Total carotenoid intake is associated with reduced loss of grip strength and gait speed over time in adults: The Framingham Offspring Study

Lower antioxidant serum concentrations have been linked to declines in lean mass and physical function in older adults. Yet population data on the effect of dietary antioxidants on loss of muscle strength and physical function are lacking. We sought to determine the association of antioxidant intake [vitamin C, vitamin E, and total and individual carotenoids (α-carotene, β-carotene, β-cryptoxanthin, lycopene, and lutein+zeaxanthin)] with annualized change in grip strength and gait speed in adults from the Framingham Offspring study. This prospective cohort study included participants with a valid FFQ at the index examination and up to 2 prior examinations and at ≥2 measures of primary outcomes: grip strength (n=2452) and/or gait speed (n=2422) measured over 3 subsequent examinations. Annualized change in grip strength (kg/y) and change in gait speed (m/s/y) over the follow-up period were used. Linear regression was used to calculate β coefficients and P values, adjusting for covariates. Mean ± SD age of participants was 61 ± 9 y (range: 33-88 y). Median intakes (IQR, mg/d) of vitamin C, vitamin E, and total carotenoid across available examinations were 209.2 (133.1-394.2), 27.1 (7.4-199.0), and 15.3 (10.4-21.3), respectively. The mean follow-up time was ∼12 ± 2 y (range: 4.5-15.4 y). In the sex-combined sample, higher intakes of total carotenoids, lycopene, and lutein+zeaxanthin were associated with increased annualized change in grip strength [β (SE) per 10-mg higher intake/d, range: 0.0316 (0.0146) to 0.1223 (0.0603) kg/y)]. All antioxidants except for vitamin C were associated with faster gait speed [β (SE) per 10-mg higher intake/d, range: 0.00008 (0.00004) to 0.0187 (0.0081) m/s/y]. Higher antioxidant intake was associated with increase in grip strength and faster gait speed in this cohort of adults. This finding highlights the need for a randomized controlled trial of dietary antioxidants and their effect on muscle strength and physical function.