Insulin-like Growth Factor-1 Gene Polymorphism Research Articles

Polymorphism analysis of the Insulin-like Growth Factor-1 (IGF-1) gene encoding growth in F5 Golden Kamper chicken (Gallus gallus domesticus)

Golden Kamper Chicken is a chicken cross between pelung and layer chickens with good meat and egg productivity. Insulin-like growth factor-1 (IGF-1) gene is one factor affecting the speed of chicken growth. This research aims to study the character of F5 Golden Kamper chickens and analyze the IGF-1 gene to determine its effect on chicken growth. The research will be done by hybridizing the F4 Golden Kamper hen and rooster, raising brood stock and DOC, egg collection, growth data collection, blood collection, DNA isolation, DNA amplification, electrophoresis, and sequencing. The observed parameters are DOC weight for the first seven weeks and polymorphism of the IGF-1 gene. Data was analyzed using Microsoft Excel, Genestudio, MEGA11, and IBM SPSS 25 software. Sequencing data were processed using the Genestudio application, alignment with MEGA11, and genotype-haplotype associations using Pearson correlation and Linear Regression F test. The results showed that the molecular marker polymorphism of the IGF-1 gene was found at 12 mutation points. IGF-1 gene polymorphism did not correlate with the weight of F5 Golden Kamper chickens.

Association of rs5742612 Polymorphism in the Promoter Region of IGF1 Gene with Nonalcoholic Fatty Liver Disease: A Case-Control Study.

Nonalcoholic fatty liver disease (NAFLD) is an emerging global chronic liver disease encompassing a wide spectrum of disorders ranging from simple steatosis to nonalcoholic steatohepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma. Considering the strong association between NAFLD and insulin resistance, and the vital role of insulin-like growth factor 1 (IGF1) in IR, we hypothesized that IGF1 gene polymorphism might be associated with NAFLD. A total of 302 subjects, including 149 patients with biopsy-proven NAFLD and 153 controls, were enrolled in this case-control study. All the subjects were genotyped for the rs5742612 polymorphism of the IGF1 gene using the polymerase chain reaction-restriction fragment length polymorphism method. The distribution of IGF1 rs5742612 genotypes and alleles differed significantly between the cases with NAFLD and controls. The IGF1 rs5742612 CC genotype compared with the TT genotype or the TT+TC genotype occurred more frequently in the cases than the controls and the differences remained significant after adjustment for confounding factors such as age and body mass index (P = .011, OR = 2.71, 95%CI = 1.16-5.85; and P = .032, OR = 2.29, 95% CI = 1.10-5.24, respectively). For the first time, this study uncovered that the IGF1 rs5742612 CC genotype compared with the TT genotype or the TT+TC genotype had a 2.71-fold or 2.29-fold increased risk for NAFLD, respectively.

Association of the Combined Effects between Insulin-Like Growth Factor-1 Gene Polymorphisms and Negative Life Events with Major Depressive Disorder among Chinese population in the Context of Oxidative Stress

Background Oxidative stress may be increased in a number of psychiatric disorders, including major depressive disorder (MDD). MDD has been shown to be related to insulin-like growth factor-1 (IGF-1) as well as to negative life events; exploring the interaction of IGF-1 polymorphisms and negative life events on the risk of MDD is needed. The aim of this study was to analyze the single and combined effects of IGF-1 polymorphisms (rs972936 and rs978458) and negative life events with MDD among Chinese population. Methods 420 MDD patients (according to DSM-V) and 420 age- and gender-matched control subjects were recruited in a case-control study. Negative life events were assessed using standard rating scales. IGF-1 rs972936 and rs978458 were identified by sequencing. The chi-square (χ2) tests were performed to explore the association of negative life events and IGF-1 polymorphisms with MDD. Results Our results found that the negative life events were associated with the risk of MDD (P < 0.001; OR = 3.28, 95% CI: 2.19-4.85). The genotypes of IGF-1 were associated with the risk of MDD (P < 0.001); carrying the IGF-1 rs972936 C allele (OR = 1.53, 95% CI: 1.26-1.85) and rs978458 T allele (OR = 1.92, 95% CI: 1.58-2.34) had a higher risk of MDD. The combined effects between IGF-1 rs978458 and negative life events were associated with the risk of MDD (P < 0.05; OR = 2.94, 95% CI: 1.23-7.03), but IGF-1 rs972936 was not associated (P > 0.05). Conclusions Based on the oxidative stress hypothesis, we confirm that carrying IGF-1 rs972936 C allele and rs978458 T allele have a higher risk of MDD and the combined effects between IGF-1 rs978458 and negative life events were associated with the risk of MDD among Chinese population.

Association of insulin-like growth factor 1 (IGF1) gene polymorphism with the reproductive performance of three dual-purpose chicken breeds.

The study was designed to investigate the association of Insulin-like growth factor 1 (IGF1) gene polymorphism with the reproductive performance of FUNAAB-Alpha, Sasso, and Kuroiler dual-purpose chicken breeds. To achieve this, a total of 250 healthy hens were selected at 12 wk of age and were intensively managed in cages for 52 wk. Blood sample was taken from each chicken at the 34th week and genomic DNA was extracted using Qiagentm DNA extraction kit, PCR was used to amplify the DNA fragments, and the PCR products were electrophoresed. Amplicons obtained were digested with restriction enzyme hinf1, and were further electrophoresed on 1.5% agarose gel. Data obtained were analyzed using the General linear model of SAS (2002) version 9.0 to determine the effect of IGF1 gene polymorphism and the distribution of alleles within the breeds. Results show polymorphism of the IGF1 gene and the restriction analysis indicated two alleles; A 58% and C 42% with the identification of genotypes AA, AC, and CC, and genotypic frequency of 22%, 43%, and 35%, respectively. Significant associations were observed between the polymorphism of the IGF1 gene, age of the bird at first lay, and weight of the hen at first lay. Chickens with haplotype CC came earlier into lay compared to those with the other two haplotypes (AA and AC). Therefore, the study suggests that haplotype CC could be used as a genetic marker to select for an improved laying performance in chickens.

IGF-1 Gene Polymorphism and its Association with Cashmere Fiber Trait in Changthangi Goats

ABSTRACT The Cashmere (Pashmina) fiber attracts great demand worldwide for its fineness. To identify and determine the role of Insulin-like Growth Factor-1 (IGF-1) gene polymorphism on Pashmina fiber traits (quality and quantity), exon IV, and part of intron IV of the IGF-1 gene of Changthangi goats were analyzed. 103 Changthangi goats of either sex were divided into two groups, the high producers and low producers of Pashmina. Upon restriction digestion with HaeIII, three genotypes were identified, i.e., A1A1 (0.253), A2A2 (0.320), and A1A2 (0.427). These genotypes did not vary significantly (p > .05) from Hardy–Weinberg Equilibrium. DNA sequence analysis revealed G > C transversion in intron-IV of IGF-1 gene at the HaeIII restriction site. The SNP effect of two alleles on mean Cashmere fiber diameter of down fibers (MFD) was statistically significant (p < .05) and the average finest fiber diameter was 13.46 µ for genotype A2A2. The mean genetic variability parameters for the gene analyzed were heterozygosity value (0.4977), PIC Value (0.3738), Effective allele no (1.9908),and Shannon index I (0.6908). The significant association of IGF-1 gene polymorphism and fiber finesse can be exploited in breeding programmes to improve the production and quality of Pashmina fiber in goat populations.

Maternal and environmental risk for faltered growth in the first 5 years for Tanjungsari children in West Java, Indonesia.

Low birth weight leads to growth faltering, attributable inter alia to malnutrition and maternal health and literacy. Risk for growth faltering in rural children under five is studied. The Risk Approach Strategy in Tanjungsari, West Java has been analysed for all pregnancies during 1988-1989 and 4,698 singleton infants born between 1 January 1988 and 31 April 1990. Weight and body length/height measurements were repeated over 60 months, and plotted against WHO standards. Weight-for-age and height-forage z-scores were calculated using 2006 WHO growth as reference. The correlation between shortness (so-called stunting) and its presumptive risk factors was determined. A subset underwent DNA analysis for insulin-like growth factor-1 (IGF-1), and insulin receptor substrate-1 (IRS-1) polymorphism. Weight and body length/height follow-ups were followed-up for 3795 infants; 14.2% of the cohort had low birth weight (<2500 g) (LBW) and 85.8% normal birth weight (NBW). LBW infants showed a similar velocity but tended to catch up more slowly (GEE; p<0.001). Relative to WHO references, the differential for stature increased with age, largely offset by reduced weight-for-age so that weight-for-height tracked close to the WHO reference; this contrasts with more divergence internationally. Birth length and weight, along with potable water access were correlated with stunting for children under 2 years. Neither the observed IGF-1, IRS-1 or combined gene polymorphisms were associated with LBW. The prediction by factors operative during pregnancy for early life stature, with some adaptation for LBW infants, endures to 60 months.

Association of IGF-I gene polymorphism with diabetic nephropathy in Egyptians with type 2 diabetes

BackgroundGenetic-based studies are being carried out worldwide to identify the susceptibility genes for diabetic nephropathy (DN), which represents a major health problem in all diabetics.AimTo study the association between genotypes of insulin-like growth factor-1 (IGF-1) and DN among Egyptian patients with type 2 diabetes and if there is any possible relation between these polymorphisms and different variables.Patients and methodsA total of 26 diabetics without nephropathy (group 1) and 26 with nephropathy (group 2), with an average age of 52.7±6.1 years, and 25 age-matched and sex-matched healthy participants (control group) were included. Two tagging single nucleotide polymorphisms were assessed in IGF-1 gene: rs6214 and rs10860860. Genotypic distribution was tested for Hardy–Weinberg equilibrium. The genotype was evaluated using the χ2 tests. Fasting blood glucose, glycated hemoglobin, uric acid, lipid profile, serum creatinine, and urine albumin–creatinine ratio were assessed.ResultsThe distribution of IGF-1 gene polymorphisms reflects a significant association with DN, where the frequency of variant genotype GG in polymorphism rs6214 was found to be significantly higher in diabetics with nephropathy than other groups [odds ratio (OR)=20.57; 95% confidence interval, 2.25–74; P=0.001). Moreover, the frequency of variant AA in polymorphism rs10860860 was also found to be significantly higher in diabetics with nephropathy (OR=7.37; 95% confidence interval, 1.87–30.07; P=0.001). However, GA and AT alleles were found to be associated with ORs less than 1 in diabetics with nephropathy when compared with other groups (P=0.002 and 0.007, respectively), which means that it could be significantly protective against DN. where they found to be significantly higher in diabetics with genotype GG than those with genotype GA (P=0.13, 0.11 respectively).ConclusionThe variants of IGF-1 rs6214 and rs10860860 could entail a risk of DN in Egyptians with type 2 diabetes mellitus. It means that the type of IGF-1 gene polymorphism is responsible for the susceptibility of DN more than its serum level.

Association between the GHR, GHRHR, and IGF1 gene polymorphisms and milk yield and quality traits in Sarda sheep

The growth hormone receptor (GHR), the growth hormone releasing hormone receptor (GHRHR), and the insulin-like growth factor 1 (IGF1) genes are known to modulate growth, reproduction, and lactation traits in livestock. The aim of the current work was to investigate if the variation of the sheep GHR, GHRHR, and IGF1 genes is associated with milk yield and quality traits. Three hundred eighty dairy Sarda sheep were genotyped for 36 single nucleotide polymorphisms (SNP) mapping to these 3 loci, and records for milk yield and daily fat and protein yield, as well as for fat, protein, casein, lactose, and milk urea contents, pH, somatic cell score, logarithmic bacterial count, and milk energy were obtained. The linkage disequilibrium analysis was performed only for GHR, as both GHRHR and IGF1 had only 1 polymorphic SNP. Haplotype analysis revealed the existence of 7 haplotype blocks in GHR. Two haplotype blocks, including part of the intron 1 and the upstream region, were clearly separated from the remaining 5 blocks by SNP rs412986330, which may be a recombination hotspot. The latter 5 blocks were contiguous, spanning from intron 2 to exon 10. Statistical analysis revealed that the GHR polymorphism is significantly associated with milk traits for daily fat and protein yield and fat, milk urea, and lactose content. Moreover, variation in IGF1 was associated with milk protein and casein content. Data generated in this research provide new insights into the allelic effects of the ovine GHRHR, GHR, and IGF1 genes on milk production and quality traits, information that may be useful in gene-assisted selection programs.

Rs1520220 and Rs2195239 Polymorphisms of IGF-1 Gene Associated with Histopathological Grades in IgA Nephropathy in Northwestern Chinese Han Population

Background/Aims: Insulin-like growth factor-1 (IGF-1) plays important roles in cellular proliferation, differentiation, and growth. Previous studies showed that single-nucleotide polymorphisms (SNPs) of IGF-1 are associated with various diseases. This case-control study aimed to examine the relationship between IGF-1 polymorphisms and IgA nephropathy (IgAN) risk in a Chinese Han population. Methods: We recruited 351 IgAN patients and 310 healthy controls from Northwestern China. Sequenom MassARRAY was utilized to examine the genotypes of two common IGF-1 SNPs (rs1520220 and rs2195239). Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated by the Chi square test to evaluate the associations between IGF-1 and IgAN. Results: Our study demonstrated that IGF-1 gene rs1520220 and rs2195239 polymorphisms did not confer susceptibility to IgAN. We found no correlation between gender, blood pressure, proteinuria, eGFR, and IgAN in both SNPs. However, the rs1520220 and rs2195239 variants were correlated with M1 and E1 in patients with IgAN (M0/M1: CC vs. CG+GG: OR = 1.62, P = 0.04; E0/E1: CC vs. CG+GG: OR = 1.95, P = 0.004; GG vs. GC+CC: OR = 1.90, P = 0.004, respectively). Conclusion: These results indicate that IGF-1 gene polymorphisms play crucial roles in the histopathological progression of IgAN in the Chinese Han population.

Genetic Polymorphisms of Insulin-Like Growth Factor 1 Are Associated with Osteosarcoma Risk and Prognosis.

BackgroundInsulin-like growth factor 1 (IGF-1) gene plays an important role in bone and soft tumors. IGF-1 gene polymorphisms have been revealed to be correlated with the carcinogenesis and progression of solid malignancies. We therefore hypothesized that IGF-1 genetic polymorphisms might be associated with the risks and outcomes of osteosarcomas in Chinese individuals.Material/MethodsThis study included 173 conventional osteosarcoma individuals and 175 tumor-free controls. Five single nucleotide polymorphisms (SNPs) of IGF-1 (rs6214, rs6218, rs35767, rs5742612, and rs5742714) were genotyped. DNA was extracted from peripheral blood and analyzed for SNP genotyping using PCR.ResultsWe found that rs6218 had a predictive role for the susceptibility and progression of osteosarcoma. The presence of TC and CC genotypes of rs6218 indicated higher risk of osteosarcoma. In addition, rs6218 TC and CC genotypes were discovered to be associated with later stage and elevated risk of osteosarcoma metastasis.ConclusionsIGF-1 polymorphisms are potential prognostic predictors of osteosarcoma susceptibility and outcomes.

Association between Insulin Like Growth Factor-1 (IGF-1) gene polymorphism and carcass traits in improved Nigerian indigenous chickens

The insulin-like growth factor-1 (IGF1) is a key regulator of muscle development and metabolism in birds and other vertebrate. Our objective was to determine the association between IGF1 gene polymorphism and carcass traits in FUNAAB Alpha chicken. Genomic DNA was extracted from the blood of 50 normal feathered birds. At 10 weeks, the birds were slaughtered for carcass traits. Specific primers for chicken IGF1 were used for amplification of a 622 base segment. The amplified gene products were digested with Hinf1 restriction enzyme and the digested fragments were genotyped. Allele frequencies were 52% and 48% for A and B, respectively. Genotype frequencies were 27%, 50% and 23% for AA, AB and BB genotypes, respectively. All carcass traits values and the IGF1 gene polymorphism observed were subjected to analysis of variance and the mean were separated using Duncan Multiple Range Test. The results showed that the occurrence of the polymorphism did not affect all the carcass traits but AB genotypes had the highest carcass traits values than the AA and BB genotypes. The conclusion of this study demonstrated that IGF-1 gene, to some extent, could be a candidate gene that affects carcass traits in Improved Nigerian indigenous chicken.Keywords: IGF-1, PCR-RFLP, indigenous chicken

Insulin-like growth factor 1 gene polymorphism in women with breast cancer

Breast cancer is a disease of unknown etiology; however, the major risk factors are genetic alterations. Studies have demonstrated an association between insulin-like growth factor 1 (IGF-1) gene polymorphism and cell proliferation and reduced apoptosis, in addition to its role in breast cancer growth and aggressiveness. Two polymorphic variants of the IGF-1 gene are highlighted in association with breast cancer, rs6220 and rs7136446, although controversy exists as to this relationship. The current study included 137 women (68 breast cancer cases and 69 controls without breast cancer) who had 3ml of peripheral blood drawn for the study of genomic DNA extracted from leukocytes using the genotyping technique by real-time polymerase chain reaction. The CC genotype (rs7136446) was present in 4 women (5.9%) from the case group and in 2 (3.0%) women from the control group (p=0.67), while the GG genotype (rs6220) occurred in 8 (11.5%) women from the case group and in 5 (7.2%) women from the control group (p=0.75). No statistically significant difference was observed between the CC genotype of variant rs7136446 in premenopausal case and control women (p=0.31), thus as there was also no significant difference between case and control postmenopausal women (p=1.00). Concerning the GG genotype of rs6220, it occurred in 6 (14.2%) premenopausal case and 4 (8%) control women (p=0.71) and no difference was found in postmenopausal women (p=1.00). In the current study, IGF-1 gene polymorphism of SNP variants rs6220 and rs7136446 had no statistically significant association with breast cancer, both in premenopausal and postmenopausal women.

Insulin-like Growth Factor 1 gene polymorphism and breast cancer risk.

Insulin-like Growth Factor-1 (IGF-1) gene polymorphism has been associated with an increased risk for breast cancer. IGF-1 is a key regulator of proliferation, cell differentiation and apoptosis. It has important mitogenic and anti-apoptotic activities in normal cells and in breast cancer cells, acting synergistically with estrogen to increase neoplastic cell proliferation. This review aims to present the recent finds of IGF-1 gene polymorphism and its relationship with the risk of breast cancer through following the polymorphic dinucleotide repeat cytosine-adenine (CA) and single nucleotide polymorphisms (SNPs) by searching in the PubMed database publications focused studies published from 2010 to 2015 related to IGF-1 gene polymorphism and breast cancer risk. A growing number of studies support an association between IGF-1 gene polymorphism and breast cancer risk with conflicting results, nevertheless elucidation of the patterns of IGF-1 gene expression may permit characterization of women at high-risk for breast cancer, as well as the development of strategies for early diagnosis and efficient treatment against the disease.

Association of Insulin-Like Growth Factor-1 Gene Polymorphisms with Different Types of Myopia in Egyptian Patients.

To investigate the effect of the insulin-like growth factor-1 (IGF-1) gene's rs6214 and rs5742632 polymorphisms on IGF-1 expression levels and their association with different types of myopia in Egyptian patients. A case-control format was used that included 272 patients with myopia and 136 controls. The IGF-1 gene rs6214 and rs5742632 polymorphisms were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses. IGF-1 levels were measured by an enzyme-linked immunosorbent assay. In patients with high-grade myopia, the frequencies of the IGF-1 rs6214 GA and AA genotypes, and the A allele were significantly increased compared to the control group: 41.9% vs. 33.8%, 17.7% vs. 8.9%, and 38.9% vs. 25.7%, respectively. Subjects with the GA and AA genotypes and carriers of A allele were significantly more likely to have high-grade myopia: odds ratios (OR) = 1.75, 95% confidence interval (CI) = 1.03-2.9, and p = 0.03; OR = 2.8, 95% CI = 1.3-6.0, and p = 0.003; and OR = 1.8, 95% CI = 1.25-2.61, and p = 0.001, respectively. A nonsignificant association of the IGF-1 gene rs5742632 polymorphism with the two myopia groups was also observed. The IGF-1 levels were significantly increased in patients with high-grade myopia and simple myopia compared to the control group (p < 0.05). In addition, our results showed a nonsignificant association of the IGF-1 (rs6214-rs5742632) haplotype with either simple myopia or high-grade myopia. We found a significant association of the IGF-1 gene rs6214 polymorphism in Egyptian patients with simple myopia and high-grade myopia. IGF-1 levels were significantly increased in relation to the IGF-1 rs6214 genotypes, while a nonsignificant association was found between IGF-1 level and the IGF-1 (rs5742632) genotypes.

Association of Insulin-Like Growth Factor-1 Gene Polymorphism at 279 Position of the 5’UTR Region with Body Weight Traits in Broiler Chicken

Association of Insulin-Like Growth Factor-1 Gene Polymorphism at 279 Position of the 5’UTR Region with Body Weight Traits in Broiler Chicken

Association of insulin-like growth factor-1 gene polymorphism with psoriasis

Association of insulin-like growth factor-1 gene polymorphism with psoriasis

A Novel Single Nucleotide Polymorphism in Exon 4 of Insulin-Like Growth Factor-1 Associated with Production Traits in Bali Cattle

Insulin-like growth factor-1 (IGF-1) is one of the gene candidates that can be used in selection strategy by using DNA markers (marker assisted selection). Gene candidate strategy is a molecular biology techniques to identify quantitative trait loci directly, with the assumption that genetic variation associated to quantitative trait variation. This study was designed to identify any new mutations in exon 4 that can cause the IGF-1 gene polymorphism and then affect the production traits on Bali cattle. Single nucleotide polymorphism ( SNP) discovery was conducted by using the direct sequencing technique. Genetic variation of the genes candidate was identified by using PCR-RFLP technique. The results of this study indicate the presence of a new SNP in exon 4 of IGF-1 gene caused by the T/C transition, which can be identified using Rsa 1 restriction enzyme. Genotypic polymorphism of IGF-1/ Rsa 1 has a significant influence on birth weight, weaning weight and average daily gain of Bali cattle. CC genotype had a birth weight rate, weaning weight and average daily gain of: 15.64±1.83; 83.15±9.00, and 0.439±0.07 respectively, higher than the TT and CT genotype. IGF-1/ Rsa 1 can be used as a genetic marker for selection of birth weight, weaning weight, and daily body weight gain.

No Effect of the IGF-1 Gene rs35767 and rs17032362 Polymorphisms in the Etiology of Idiopathic Short Stature

Idiopathic short stature (ISS) refers to pathophysiologically wide and heterogeneous range of disorders, which are considered to involve defects in growth hormone (GH) insulin like growth factor-1 (IGF-1) axis. This study was designed to evaluate GH- IGF-1 axis and investigate IGF-1 gene polymorphisms in ISS.108 patients with a mean age of 11.7±3.6 years constituted the study group, while 108 age and gender matched children with normal stature constituted the control group. Serum IGF-1 and insulin-like growth factor binding protein-3 (IGFBP-3) levels and two polymorphisms in IGF-1 gene (rs35767, rs17032362) were investigated.While mean IGF-1 SDS value was lower in study group (p=0.002), no difference was detected between mean IGFBP-3 SDS values. The IGF-1 gene rs35767 polymorphism genotype distribution did not exhibit a statistical difference between study (7.1% wild type, 29.6% heterozygous, 63.3% homozygous) and control groups (3.8% wild type, 39.6% heterozygous, 56.6% homozygous). IGF-1 gene rs17032362 polymorphism genotype distribution was not significantly different either between study (94.8% wild type, 5.2% heterozygous, 0% homozygous) and control groups (97.2% wild type, 2.8% heterozygous, 0% homozygous). Comparing the cases with wild type, homozygous and heterozygous carriers for both polymorphisms with respect to height, weight, BMI, IGF-1 and IGFBP-3 SDS values, no significant difference was detected.IGF-1 SDS levels of patients with ISS were significantly lower compared to control group. There was no difference between IGFBP-3 SDS levels. No effect of IGF-1 gene rs35767 and rs17032362 polymorphisms on stature, IGF-1 and IGFBP-3 levels could be demonstrated.

Insulin-like growth factor-1 gene polymorphism in rheumatoid arthritis patients

Objectives: Insulin-like growth factor-1 (IGF-1) regulates several biological functions, and low plasma levels of IGF-1 are known to contribute towards the pathogenesis of rheumatoid arthritis (RA). In view of the biological significance of IGF-1, we investigated the association of RA with the polymorphism of a 192-bp allele which is cytosine–adenosine repeat located 1 kb upstream from the IGF-1 gene transcription site and is known to regulate serum IGF-1 levels.Methods: Blood samples were collected from 52 healthy controls (HC) and 68 RA patients to measure the levels of IGF-1 and to isolate genomic DNA. Polymorphism of the IGF-1 gene was examined using polymerase chain reaction (PCR). Disease severity, duration, and activity were recorded for all RA patients.Results: We observed that 97% of all the subjects who participated in this study showed the presence of a 192-bp allele of the IGF-1 gene. All healthy controls exhibited the presence of 192-bp wild-type allele. All non-carriers of the 192-bp allele were Arabs and had RA. Gender correlated significantly with allele frequencies as 14% of the male and only 2% of the female RA patients were non-carriers of 192-bp allele. Plasma IGF-1 levels were significantly lower (p < 0.01) in RA patients compared to HC, and all RA patients who were non-carriers of the 192-bp allele had a significantly high disease activity score. No correlation was found between the duration of RA and the presence or absence of this allele.Conclusions: This study suggests a possible association of the IGF-1 gene polymorphism with developing RA, particularly in males as non-carriers of the 192-bp allele.

Abstract 878: Comorbidities in African American and Hispanic/Latina Breast Cancer Patients are associated with IGF-1 and IGFBP3-3 Gene Polymorphisms

Abstract Increase in comorbidities such as diabetes and hypertension in cancer patients lead to poor disease free survival and overall survival. It is probable that risk factors associated with these comorbidities and cancer itself could be linked to alterations in gene polymorphisms associated with hormones and/or growth factors. In this study, we have investigated if (1) comorbidities were associated with our African American and Hispanic breast cancer subjects; and (2) if there was any association between Insulin-Like Growth Factor 1 (IGF-1), and IGF Binding Protein 3 (IGFBP3) gene polymorphisms with breast cancer and comorbidities. We studied 514 subjects (175 African Americans and 339 Hispanic/Latinas), which included 208 cases and 306 controls (no evidence of breast cancer) subjects. DNA was extracted from blood samples and IGF-1 and IGFBP-3 genotyping was performed by PCR-GeneScan and PCR-RFLP methods, respectively. Our results demonstrated that comorbidity is significantly correlated with breast cancer (OR = 2.12; 95% CI = 1.40-3.21; P-value &amp;lt;0.01); especially the comorbidity of hypertension (OR= 2.27; 95% CI = 1.47-3.49; P-value &amp;lt;0.01). Furthermore, there was a significant association between non-19/non-19 IGF-1 (CA)n polymorphisms and breast cancer (OR= 4.80; 95% CI = 1.87, 12.36; P-value &amp;lt;0.01). This study also found a significant association between the non-19/non-19 IGF-1 (CA)n genotype, breast cancer, and comorbidity (OR= 4.65; 95% CI = 1.57, 13.82; P-value &amp;lt;0.01). In summary, our study demonstrated a significant association of IGF-1 polymorphisms and breast cancer with a further link to comorbid diseases. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 878.