Abstract
The insulin-like growth factor-1 (IGF1) is a key regulator of muscle development and metabolism in birds and other vertebrate. Our objective was to determine the association between IGF1 gene polymorphism and carcass traits in FUNAAB Alpha chicken. Genomic DNA was extracted from the blood of 50 normal feathered birds. At 10 weeks, the birds were slaughtered for carcass traits. Specific primers for chicken IGF1 were used for amplification of a 622 base segment. The amplified gene products were digested with Hinf1 restriction enzyme and the digested fragments were genotyped. Allele frequencies were 52% and 48% for A and B, respectively. Genotype frequencies were 27%, 50% and 23% for AA, AB and BB genotypes, respectively. All carcass traits values and the IGF1 gene polymorphism observed were subjected to analysis of variance and the mean were separated using Duncan Multiple Range Test. The results showed that the occurrence of the polymorphism did not affect all the carcass traits but AB genotypes had the highest carcass traits values than the AA and BB genotypes. The conclusion of this study demonstrated that IGF-1 gene, to some extent, could be a candidate gene that affects carcass traits in Improved Nigerian indigenous chicken.Keywords: IGF-1, PCR-RFLP, indigenous chicken
Highlights
The major objective of any animal breeder is to select superior animals for breeding purpose (Hossein and Mohsen, 2011)
The analysis of variance of effects of IGF-1 gene polymorphism on carcass traits showed that it had no significant (P>0.05) effect on the live body weight before slaughter (1000.63±97.02 g), the bled weight (964.86±93.89 g), the plucked weight (904.87±87.87), the eviscerated weight (722.93±73.75 g), the thigh weight (102.30±11.04), the breast weight (154.70±17.60) and the back weight (145.96±16.12) for genotype AB, higher mean values were observed in these and every other traits considered but the effects were not significant (Table 1)
Polymorphism of IGF-1 shows that it may be a potential candidate gene associated with growth, body composition and carcass traits of chicken (Zhou et al, 2005)
Summary
The major objective of any animal breeder is to select superior animals for breeding purpose (Hossein and Mohsen, 2011). This is because variation exists among animals including poultry. Nigeria indigenous chickens are known to be dual-purpose bird that is used both for meat and egg production in the rural and peri-urban area of the country. They are found in large numbers distributed across different agro ecological categories under a traditional family scavenging management system (Sonaiya and Olori, 1990). The Nigeria indigenous chicken has been said to be small bodied, slow growing, poor feed converters and poor meat animals (Nwosu and Asuquo, 1985)
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