Insulin In Rats Research Articles

Ameliorative property of Sesbania grandiflora on carbohydrate metabolic enzymes in the liver and kidney of streptozotocin-induced diabetic rats.

In diabetic condition, endogenous glucose synthesis will be elevated due to defect in the action of vital enzymes involved in carbohydrate metabolism, which is the main cause for hyperglycemia. The current study was designed to explore the anti-hyperglycemic efficacy of Sesbania grandiflora flower (SGF) extract by evaluating the concentration of C-peptide, insulin, glucose, glycosylated hemoglobin (HbA1C), hemoglobin (Hb), glycogen and carbohydrate metabolic enzymes activities in diabetic rats. The study found to lower the level of glucose, HbA1C and simultaneously ameliorated concentrations of C-peptide, insulin, hemoglobin (Hb), glycogen and carbohydrate metabolic enzymes activities in SGF treated (250 mg/kg body weight for 45 days) diabetic rats. Moreover, SGF administered diabetic rats showed diminished consumption of food and water at the same time improved body weight. The results obtained from the present study were compared with glibenclamide treated (600 µg/kg body weight) diabetic rats. SGF were supplemented to normal rats to rule out toxic effect of SGF, to explore any significant alteration in the above parameters. Hence, the results depict that SGF modulated the carbohydrate metabolic enzymes activities through ameliorating the secretion of insulin and diminishing the level of glucose concentration in STZ-induced diabetic rats by its bioactive compounds.

Antihyperlipidemic effect of tyrosol, a phenolic compound in streptozotocin-induced diabetic rats

We investigated the antihyperlipidemic effects of tyrosol in streptozotocin (STZ)-induced diabetic rats. Rats were injected intraperitoneally with STZ (40 mg/kg), and these established experimental rats were treated with tyrosol (20 mg/kg) and glibenclamide (600µg/kg) for 45 days. The observed results revealed that tyrosol treatment significantly reduced plasma glucose, plasma, and liver total cholesterol, triglycerides, free fatty acids, phospholipids, plasma low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, atherogenic index, and significantly increased plasma insulin and high-density lipoprotein cholesterol in STZ-induced diabetic rats. The activity of 3-hydroxy 3-methylglutaryl coenzyme A reductase significantly reduced in the liver, whereas the activities of lipoprotein lipase and lecithin cholesterol acyltransferase were significantly increased in the plasma of tyrosol treated STZ-induced diabetic rats. Histological examination showed that tyrosol treatment remarkably reduced lipid accumulation in the liver of STZ-induced diabetic rats. The present study revealed that tyrosol exhibits potent antihyperlipidemic effects in STZ-induced diabetic rats.

Maintenance Impact of Large Dose of Vitamin C on Proinflammatory Cytokines, Insulin, and Electrocardiographic Parameters of Wistar Rats Following Partial Pancreatectomy.

We investigated serum tumor necrosis factor alpha (TNF-α) and interleukins (IL-6 and IL-8) in rats undergoing pancreatic wound healing after partial pancreatectomy. In addition, we studied the effects of partial pancreatectomy on the insulin and the electrocardiography (ECG). We proposed that vitamin C (VitC) could have maintenance impact on TNF-α, IL-6, IL-8, insulin, and ECG parameters of pancreatic wound healing of Wistar rats that had partial pancreatectomy surgery, if administered in large dose. Thirty-five male adult Wistar rats (180-250 g) were randomized into 7 groups, with 5 rats in each group. Group 1 was control. Groups 2, 3, and 4 (phase 1) received oral 1,000 mg/kg VitC, while groups 5, 6, and 7 (phase 2) received only water and feed ad libitum postoperatively for 14 days. One-quarter (¼) pancreatectomy was performed in groups 2 and 5, half (½) pancreatectomy was performed in groups 3 and 6, and three-quarter (¾) pancreatectomy was performed in groups 4 and 7. Significant (P < 0.5) decrease in IL-6 was observed in phase 1 when compared with the control. Significant increase in IL-6 was observed when compared with control. Significant increase in IL-8 was observed in phase 1 (groups 2 and 3) and phase 2 when compared with the control. Significant decrease in TNF-α was observed in phase 1 when compared with the control. Significant decrease in TNF-α was observed in phase 2 (groups 6 and 7) when compared with the control. Insulin level decreased and increased insignificantly in phase 2 and phase 1, respectively, when compared with the control. Although atrial fibrillation was recorded in phase 2 (group 7), normal ECG was seen in the control and phase 1 (group 2). Large dose vitC may be helpful in the reduction of proinflammatory cytokines as well as elevation of insulin and normalization of ECG in rats that had undergone partial pancreatectomy.

The Effects of Grape Seed Extract on Insulin, Adiponectin and Resistin Levels in Diabetic Rats

In this study, the effects of grape seed extract on insulin, adiponectin and resistin levels in diabetic rats were aimed to investigate. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats were used. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats randomly divided into four groups of eight each: control group (C), group with diabetes (DM), grape seed extract group (GSE), diabetes mellitus and grape seed extract group (DM+GSE). The diabetic group 45 mg/kg single dose of streptozotocin was administered intraperitoneally, and the grape seed extract groups (DM+GSE and GSE) grape seed extract was given orally every day with an intragastric tube for 20 days (0.6 ml/rat). Blood samples were taken from the hearts of rat‘s end of the experiment. The sera obtained were used for insulin, adiponectin and resistin analysis measured via ELISA with commercial kits. The insulin and adiponectin levels of the rats in the diabetes + grape seed extract group were found to be higher than those in the diabetes group. There was no significant change in the resistin level differences between the groups. As a result; It is possible to say that grape seed extract has an increasing effect on insulin level and adiponectin levels of diabetic rats are decreased due to diabetes and it has no significant effect on resistin levels. Detected by the positive effects of grape seed extracts, it may be useful to use for the regulating of the diabetes in human.

Putative Anti-Immobility Action of Acute Insulin Is Attributable to an Increase in Locomotor Activity in Healthy Wistar Rats

Background/Aims: Anti-immobility actions of insulin in diabetic rats that are subjected to the forced swim test (FST) have been reported. In this test, low doses of antidepressants exert actions after long-term treatment, without affecting locomotor activity in healthy rats. Few studies have compared acute and chronic actions of insulin with antidepressants in healthy rats. Methods: We hypothesized that if insulin exerts a true anti-immobility action, then its effects must be comparable to fluoxetine in both a 1-day treatment regimen and a 21-day treatment regimen in healthy, gonadally intact female Wistar rats. Results: The results showed that low levels of glycemia were produced by all treatments, including fluoxetine, and glycemia was lower in proestrus-estrus than in diestrus-metestrus. None of the treatments or regimens produced actions on indicators of anxiety in the elevated plus maze. Insulin in the 1-day regimen increased the number of crossings and rearings in the open field test and caused a low cumulative immobility time in the FST. These actions disappeared in the 21-day regimen. Compared with the other treatments, fluoxetine treatment alone or combined with insulin produced a longer latency to the first period of immobility and a shorter immobility time in the chronic regimen in the FST, without affecting locomotor activity, and more pronounced actions were observed in proestrus-estrus (i.e., a true anti-immobility effect). Conclusion: These results indicate that insulin does not produce a true antidepressant action in healthy rats. The purported antidepressant effects that were observed were instead attributable to an increase in locomotor activity only in the 1-day regimen.

Dietary polyvinyl alcohol and alginate nanofibers ameliorate hyperglycemia by reducing insulin and glucose-metabolizing enzyme levels in rats with streptozotocin-induced diabetes.

Background and Aim:Dietary management and antidiabetic drugs are used as therapies for diabetic patients worldwide. Alginate nanofibers were developed as a digestible food product that provides sufficient calories. This study aimed to evaluate the effect of polyvinyl alcohol (PVA) and alginate nanofibers on weight gain, blood glucose levels, and insulin and other serum parameters in diabetic rats.Materials and Methods:A total of 24 male Wistar rats were divided into six groups: (C−) Control group, (C+) diabetic rats, (T1) diabetic rats + fasting treatment for 12 h, (T2) diabetic rats + nanofibers ad libitum, (T3) diabetic rats + metformin + nanofibers ad libitum, and (T4) diabetic rats + metformin. All groups were treated for 21 days. Weight gain was evaluated by comparing initial and final weights. Blood glucose levels were evaluated weekly. Serum parameters were also evaluated at the end of the study. All variables were analyzed statistically using analysis of variance followed by Tukey’s post hoc test (p<0.05).Results:The T2, T3, and T4 groups showed a significant increase in weight compared to that of the C+ and T1 groups. The T3 group had the lowest blood glucose level of all groups at the end of the study. In the serum evaluation, the T2 and T3 groups showed a significant decrease compared to the C+ group for the following variables: Alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyltransferase (GGT), creatinine, tumor necrosis factor-alpha (TNF-α), and interleukin-1β (IL-1β). In contrast, the T2 group showed a significant decrease compared to the T3 group for aspartate aminotransferase and insulin levels.Conclusion:PVA and alginate nanofibers can modulate obesity, reduce blood glucose levels, and reduce serum levels of insulin, ALT, ALP, GGT, creatinine, TNF-α, and IL-1β in diabetic rats.

Effect of early postnatal exposure to 17α-ethinylestradiol on female rat reproductive physiology

17α-Ethinylestradiol (EE2) is a synthetic compound used as an oral contraceptive in birth control pills. EE2 is a major contaminant in aquatic environments. It is considered an endocrine-disrupting chemical due to its oestrogenic activity. This study investigates the effect of repeated exposure to EE2 at a dose of 1 µg/kg·(body weight)/day on reproductive function and metabolism. Female Sprague–Dawley rats were subcutaneously injected with EE2 at a dose of 1 µg/kg·(body weight)/day from postnatal day 1 until postnatal day 5, which corresponds to a highly sensitive period of neuroendocrine system organisation. The anogenital distance, body weight and blood levels of insulin and glucose of EE2-treated female rats were not different from those of control rats. These results indicate that growth and glucose metabolism were unaffected by treatment. In contrast, vaginal opening occurred significantly earlier in EE2-treated females than in control females. In adulthood, these females present an alteration of their oestrous cyclicity, characterised by persistent vaginal oestrus. Moreover, treated females showed altered uterine and ovarian morphology associated with lower circulating levels of oestradiol and progesterone. These results indicate precocious onset of puberty and dysfunction of the reproductive organs. Therefore, early postnatal exposure to EE2 at a dose of 1 µg/kg·bw/d has delayed adverse effects on reproductive function.

Effects of date seeds administration on anxiety and depressive symptoms in streptozotocin-induced diabetic rats: biochemical and behavioral evidences.

Several epidemiological data indicate that chronic hyperglycemia is associated with behavioral changes such as anxiety and depressive symptoms. Date seeds, one of the most potent products with potential antioxidant activities and possess many benefits against hyperglycemia and its complication. The aim of the current study was to explore the potential effect of date seeds extract on biochemical and behavioral changes (anxiety and depression) in streptozotocin (STZ)-diabetic rats. Rats were divided into four groups as follows: normal control, diabetic control, diabetic treated with the lyophilized aqueous extract of the date seed (2,000mg/kg) (LAE-DS) and diabetics treated with insulin (4UI/day). Experimental diabetes was induced by a single intraperitoneal injection of STZ (60mg/kg). After 24days treatment period, anxiety and depressive behaviors were evaluated using four behavioral tests. After sacrifice, blood samples were collected to evaluate lipid parameters. In addition, rat organs (kidney, liver and brain) were dissected out in order to estimate lipid peroxidation levels as oxidative stress marker. Oral administration of the lyophilized aqueous extract of date seeds and insulin injection for 30days significantly decreased blood glucose levels in STZ-diabetic rats and protected them against undesirable changes in lipid parameters, including cholesterol, triglycerides, LDL cholesterol, VLDL cholesterol and atherosclerosis index. Compared to untreated diabetic rat, a significant decrease in lipid peroxidation levels in kidney, liver and brain (Hippocampus and prefrontal cortex) were observed after treatment with insulin or LAE-DS in diabetic rats. Furthermore, insulin and LAE-DS administration prevented anxiety-related behaviors in STZ-diabetic rats. Therefore, it would be possible to combine this extract with insulin and use it as an antioxidant supplement for type 1 diabetic patients.

Humanin Alleviates Insulin Resistance in Polycystic Ovary Syndrome: A Human and Rat Model-Based Study.

Polycystic ovary syndrome (PCOS), the most common endocrine disorder in women of reproductive age, is characterized by hyperandrogenism and insulin resistance (IR); however, the pathogenesis of local ovarian IR in PCOS remains largely unclear. Humanin, a mitochondria-derived peptide, has been reported to be associated with IR. Our previous study confirmed that humanin is expressed in multiple cell types in the ovary and is present in follicular fluid. However, it remains unknown whether humanin participates in the pathogenesis of local ovarian IR or whether humanin supplementation can improve IR in PCOS patients. In this study, we compared humanin concentrations in follicular fluid from PCOS patients with and without IR. We further investigated the effect of humanin analogue (HNG) supplementation on IR in a rat model of dehydroepiandrosterone-induced PCOS. Humanin concentrations in the follicular fluid were found to be significantly lower in PCOS patients with IR than in those without IR. HNG supplementation attenuated both the increases in the levels of fasting plasma glucose and fasting insulin in rats with PCOS and the decreases in phosphorylation of IRS1, PI3K, AKT, and GLUT4 proteins in the granulosa cells of these rats. Combined supplementation with HNG and insulin significantly improved glucose consumption in normal and humanin-siRNA-transfected COV434 cells. In conclusion, downregulated humanin in the ovaries may be involved in the pathogenesis of IR in PCOS, and exogenous supplementation with HNG improved local ovarian IR through modulation of the IRS1/PI3K/Akt signaling pathway in a rat model. This finding supports the potential future use of HNG as a therapeutic drug for PCOS.

α-Mangosteen from Garcinia Mangostana Linn and its Effect in Blood Insulin and Sugar Levels in Hyperglycemic Rat

Introduction: Hyperglycemic triggers various complications, some organs that damage can occur due to the oxidation by oxidant of proteins both structural and non-structural, such as eyes, kidneys, heart, nerves and blood vessels. α-mangosteen as antioxidant can suppress cell damage so that blood sugar can be transported back into the cell and be found in Garcinia mangostana Linn. The purpose of this study was to explains the effect of α-mangosteen in increasing blood insulin and reducing blood sugar levels in hyperglycemic rat. Method: This study uses 9 rat treatment groups. Group I as control, group II as negative control (hyperglycemic), group III as positive control (hyperglycemia + Glybenclamide), group IV-VI treated with extract mangosteen in ethanol rind (EEMR) and group VII-IX treated with isolate α-mangosteen rind (IAMR). After 28 days peripheral blood was taken and measurements the insulin and glucose blood serum level. Results: Mean insulin control is 19.08µU/mL, after administration of STZ-NA is 6.18µU/mL, after administration with Glibenclamide is 11.58µU/m, after administration with EEMR is 18.69µU/mL. and after administration with IAMR is 17.81µU/mL. Mean glycemic control is 99.90mg/dl, after administration of STZ-NA is 259.90 mg/dl, after administration with Glibenclamide is 123.39 mg/dl, after administration with EEMR is 108.87mg/dl. and after administration with IAMR is 92.52 mg/dl. Showed an increase in insulin serum levels after administration with extract mangosteen and isolate α-mangosteen. Anova Test, showed there was a very significant difference between control and treatment in insulin levels (p=0.001). Conclusion: α-mangosteen can increasing blood insulin and reduce blood sugar levels in hyperglycemic rat.

Cerebral Insulin Bolus Revokes the Changes in Hepatic Lipid Metabolism Induced by Chronic Central Leptin Infusion.

Central actions of leptin and insulin on hepatic lipid metabolism can be opposing and the mechanism underlying this phenomenon remains unclear. Both hormones can modulate the central somatostatinergic system that has an inhibitory effect on growth hormone (GH) expression, which plays an important role in hepatic metabolism. Using a model of chronic central leptin infusion, we evaluated whether an increase in central leptin bioavailability modifies the serum lipid pattern through changes in hepatic lipid metabolism in male rats in response to an increase in central insulin and the possible involvement of the GH axis in these effects. We found a rise in serum GH in leptin plus insulin-treated rats, due to an increase in pituitary GH mRNA levels associated with lower hypothalamic somatostatin and pituitary somatostatin receptor-2 mRNA levels. An augment in hepatic lipolysis and a reduction in serum levels of non-esterified fatty acids (NEFA) and triglycerides were found in leptin-treated rats. These rats experienced a rise in lipogenic-related factors and normalization of serum levels of NEFA and triglycerides after insulin treatment. These results suggest that an increase in insulin in leptin-treated rats can act on the hepatic lipid metabolism through activation of the GH axis.

Superiority of Purple Okra (Abelmoschusesculentus) to Green Okra in Insulin Resistanceand Pancreatic β Cell Improvement in DiabeticRats.

Antidiabetic medicinal plants are increasingly used in the treatment of diabetes as they are generally assumed to pro-duce minimal side effects. Okra is a quercetin-containing plant which can induce pancreas regeneration and has antidiabetic effect. There has been a lot of research that demonstrate that purple okra contains more quercetin than green okra. To demonstrate the advantages of purple okra over green okra on the diabetic markers improvement in diabetic rats. Fifteen male 2-month-old Wistar rats were injected intraperitoneally with 65 mg streptozotocin and 110 mg niacinamide. Their blood glucose levels were measured three days after the injection. The induction of diabetes was deemed successful if the glucose level of the rats got higher than 250 mg/dL, and then such rats were considered diabetic. The diabetic rats were divided into three groups: an acarbose group, a purple okra powder group, and a green okra powder group. The latter two were given, respectively, purple and green okra powder for 28 days. Blood serum was taken to examine the fasting blood glucose, insulin, HOMA-B and GLUT-4 levels. Pancreas was examined histologically for damage using hematoxylin eosin staining. Fasting blood glucose, insulin, HOMA-B, and GLUT-4 levels of diabetic rats that received purple okra powder (p<0.05) were better than those of the rats that received green okra powder. The least damage (p<0.05) to pancreatic beta cells was found in the purple okra powder group. Purple okra is superior to green okra in terms of improving the diabetic markers of rats.

Regulatory effects of hawthorn polyphenols on hyperglycemic, inflammatory, insulin resistance responses, and alleviation of aortic injury in type 2 diabetic rats

Hawthorn polyphenol extract (HPE) is beneficial for patients with type 2 diabetes (T2D). However, the mechanism underlying its beneficial effects remains unclear. We investigated the inhibitory effects and mechanisms of HPE on insulin resistance, inflammation, and aortic injury in T2D rats, using metformin (MF) as a positive control. High-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) was used to determine the primary polyphenols in HPE. Hematoxylin & Eosin (H&E) staining was used to evaluate pathological conditions of the skeletal muscle, liver, and aorta vessels in each group. The levels of serum and intestinal tissue oxidative stress, tumor necrosis factor α (TNF-α), and inflammatory interleukin-6 (IL-6) were also assessed. Western blotting was used to evaluate protein expression levels in the associated molecular pathway. Volatile organic compounds (VOCs) from colon contents were determined using headspace-gas chromatography-ion mobility chromatography. Our results showed that supplementation with 300 mg HPE/kg body weight over four weeks significantly improved total cholesterol (TC), total triglyceride (TG), insulin, and lipopolysaccharide (LPS) levels in diabetic rats (p < 0.01). The lesions of skeletal muscle, liver, and aorta in diabetic rats were significantly improved. HPE supplementation also significantly downregulated the inflammatory factors (IL-6, TNF-α, and MCP-1) in the liver of diabetic rats via the SIRT1/AMPK/NF-κB signaling pathway. Furthermore, HPE significantly reduced insulin resistance in T2D rats by upregulating the phosphorylation of glucose absorption protein (GLUT4) and insulin resistance-associated proteins, p-IRS1, p-AKT, and p-PI3K, in the rat liver (p < 0.01). The findings show that HPE could also alleviate aortic injury by activating SIRT1 and regulating the NF-κB and Wnt2/β-catenin signaling pathways. Overall, the results of this study suggest that both HPE and MF have similar inhibitory effects on T2D in rats and that HPE could be used as a functional food component in the adjuvant treatment of T2D.

Peripheral metabolic effects of ozone exposure in healthy and diabetic rats on normal or high-cholesterol diet

Epidemiological studies show that individuals with underlying diabetes and diet-associated ailments are more susceptible than healthy individuals to adverse health effects of air pollution. Exposure to air pollutants can induce metabolic stress and increase cardiometabolic disease risk. Using male Wistar and Wistar-derived Goto-Kakizaki (GK) rats, which exhibit a non-obese type-2 diabetes phenotype, we investigated whether two key metabolic stressors, type-2 diabetes and a high-cholesterol atherogenic diet, exacerbate ozone-induced metabolic effects. Rats were fed a normal control diet (ND) or high-cholesterol diet (HCD) for 12 weeks and then exposed to filtered air or 1.0-ppm ozone (6 h/day) for 1 or 2 days. Metabolic responses were analyzed at the end of each day and after an 18-h recovery period following the 2-day exposure. In GK rats, baseline hyperglycemia and glucose intolerance were exacerbated by HCD vs. ND and by ozone vs. air. HCD also resulted in higher insulin in ozone-exposed GK rats and circulating lipase, aspartate transaminase, and alanine transaminase in all groups (Wistar>GK). Histopathological effects induced by HCD in the liver, which included macrovesicular vacuolation and hepatocellular necrosis, were more severe in Wistar vs. GK rats. Liver gene expression in Wistar and GK rats fed ND showed numerous strain differences, including evidence of increased lipid metabolizing activity and ozone-induced alterations in glucose and lipid transporters, specifically in GK rats. Collectively, these findings indicate that peripheral metabolic alterations induced by diabetes and high-cholesterol diet can enhance susceptibility to the metabolic effects of inhaled pollutants.

The Effect of High Intensity Intermittent Training (HIIT) on GLP-1R Expression in Pancreas Tissue and Serum Insulin of Male Wistar Rats with Type 2 Diabetes Mellitus

Objective: Insulin secretion and insulin resistance are associated with incidence and severity of type 2 diabetes mellitus (T2DM). This study aimed to assess the effect of high intensity intermittent training (HIIT) on GLP-1R expression in pancreas tissue and serum insulin of T2DM rats. Materials and Methods: In this quasi-experimental study, T2DM induced by intraperitoneal injection of streptozotocin-Nicotinamide in 16 male wistar rats aged 10 weeks (220 – 20 g). The rats were divided into control (no-training, n= 8) and HIIT (5 times/ week/12-week, n= 8) groups. GLP-1R expression in pancreas tissue, fasting glucose and serum insulin were measured 48 h after lasted exercise of exercise and control groups. Independent and paired T-test was used to compare variables between 2 groups. Results: HIIT resulted in significant increase in GLP-1R expression compared to control subjects (P-value: 0.023). Fasting glucose decreased (P-value&lt; 0.0001) and serum insulin increased (P-value&lt; 0.0001) in response to HIIT when compared to control subjects. Conclusion: HIIT can improve insulin secretion in male rats with T2DM and this improvement can be attributed to increased GLP-1R expression in pancreas tissue in response to training protocol.

A protein sandwich enables real-time in vivo biomarker measurement

Biosensors allow rapid, accurate, continuous measurement of glucose and insulin in live rats.

Berberine elevates mitochondrial membrane potential and decreases reactive oxygen species by inhibiting the Rho/ROCK pathway in rats with diabetic encephalopathy.

ObjectivesDiabetic encephalopathy (DE) is a serious complication of diabetes mainly occurring in the elderly patients. Berberine (BBR) is an isoquinoline alkaloids extracted from Coptis chinensis that is applied in the treatment of diabetes clinically. This study explored the possible mechanism of BBR in relieving DE.MethodsWistar rats were injected with streptozotocin and fed a high fat diet to establish the model of DE. The model rats were treated with BBR. The body weight, blood glucose and insulin of rats were measured, and Morris water maze test was conducted to evaluate the learning and memory abilities. The pathological conditions of cortical tissues were detected. The cortical mitochondria membrane potential (MMP) and reactive oxygen species (ROS) were monitored. The expressions of Rho/ROCK pathway-related genes of rat cortex were detected. The changes of MMP and ROS were detected after the treatment of Rho/ROCK pathway activator.ResultsThe body weight of model rats changed little, and levels of blood glucose and insulin were increased. The spatial learning and memory abilities were impaired, with disordered cortical neurons, and obvious neurons apoptosis and glia proliferation. BBR alleviated cognitive dysfunction and pathological damage in rats with DE. BBR enhanced cortical MMP and suppressed ROS. BBR treatment inhibited the Rho/ROCK pathway. Activation of the Rho/ROCK pathway reversed the effects of BBR on MMP and ROS.ConclusionBBR elevated MMP and reduced ROS in rats with DE by inhibiting the Rho/ROCK pathway. This study may offer novel insights for the management of DE.

Investigating the effect of fructose on glucose, insulin, adiponectin, leptin and triglyceride tolerance in adult laboratory rats

Investigating the effect of fructose on glucose, insulin, adiponectin, leptin and triglyceride tolerance in adult laboratory rats

Relation between Heat Stress and Some Hormones in Alloxan Induced Diabetic Rats

The current study carried out to evaluate the levels of corticosterone, epinephrine, insulin and thyroxine accompanied induced diabetes mellitus in rats. Therefore, ninty six rats were divided into two main groups: control group which exposed to normal temperature (21±2) °C and second group which exposed to high temperature (40±2)°C each main group subdivided into three subgroups exposed to temperature for one, two and four hours respectively, also each subgroup composed of healthy rats and diabetic rats equally. At the end of experiment blood samples were collected for determination of corticosterone epinephrine, insulin and thyroxine. Results revealed significant increase in corticosterone and epinephrine and insulin in heat stressed group compared with control group while there was significant decrease in thyroxine in healthy and diabetic rats in heat stressed group compared with control group.Results showed significant increase in corticosterone in diabetic rats compared with healthy rats in both groups ,while the significant increase in epinephrine and insulin in diabetic rats was only in heat stressed group.Results reported significant decrease in corticosterone and thyroxine according to the increase in heat exposure duration,while epinephrine and insulin increased according to the duration. These findings indicated that heat stress may be increase harmful effects in alloxan induced diabetic rats.

Antidiabetic activity of cobalt–quercetin complex: A new potential candidate for diabetes treatment

The development of effective, available, safe, and less costly antidiabetic synthetic preparations based on natural products is of quite interest. To our knowledge, the flavonoid-based cobalt–quercetin complex was not tested before for its antidiabetic activities in vivo. Thus, our aim was to investigate the cobalt-quercetin complex’s (CQC) antidiabetic activities compared to the reference drug insulin in diabetic rats. Diabetes was prompted in male rats via one injection of streptozotocin (STZ, 50 mg/kg). Daily, diabetic animals were treated with either a dose of CQC or insulin for 15 days. Water and food intakes, bodyweight changes, total antioxidant capacity (TAC), hemoglobin A1C (HbA1C%), nitric oxide (NO), aspartate aminotransferase (AST) and alanine aminotransferase (ALT), albumin, lipid portrait, malondialdehyde (MDA), fasting blood glucose (FBG), superoxide dismutase (SOD), uric acid, and creatinine levels were assessed. Pancreas and liver tissues were histopathologically examined. STZ-induced insulin-dependent diabetes mellitus (IDDM) developed significant elevations in FBG, HbA1C, AST, ALT, NO, creatinine, urea, uric acid, and MDA levels, along with significant reductions in albumin, TAC, and SOD levels besides marked lipid profile disturbances and tissue histopathological changes. CQC treatment effectively reversed all the studied diabetes-induced changes via its strong antioxidant properties. Antidiabetic effects of CQC and insulin were comparable. Additional validation studies on the considerable CQC antidiabetic effects are needed.