At present, the heat loss of energy-efficient buildings through transparent partitions, i.e. curtain walls and windows, make more than 40 % of the total heat loss. Since most of area of these partitions (70–80%) are covered by the insulating glass units (IGU), it accounts for the largest part of the building’s heat losses. These losses increase with the lifetime of the building as the heat transfer coefficient of the IGU increases with decreasing argon gas concentration in a cavity of glass unit, due to the ageing of the surrounding seals. While the content of Ar gases decreases, a soft low-E coating of glass is oxidizing, decreasing insulation of the radiant heat flux which determines for about 50 % of the total heat flux through the IGU. In order to make targeted decisions on replacing of degraded IGUs their insulation properties have to be evaluated using reliable in-situ measurement method. In this paper, non-invasive adaptive ultrasonic technique for assessment of Ar gas content in the gas mixture of IGU was proposed. The technique is based on the sound velocity measurements and is combined with electromagnetic field phase measurement methods for precise estimation of the thickness of IGU at any spatial position. The technique was verified on the IGU samples with known Ar gas concentrations and compared with commercial systems and gas chromatography in range of 27–93% of Ar content. It was estimated, that technique is capable of measuring Ar concentration with accuracy of ± 3.15%, which exceeds the current industry standards.