Instrumental Methods Of Analysis Research Articles

Methods of extraction, separation and identification of cyclic peptides from flaxseed (Linum usitatissimum L.): A review

Oilseed flax (Linum usitatissimum L.) is a valuable crop characterized by a high content of fats, dietary fiber, protein and various biologically active substances, in particular cyclopeptides. Cyclic peptides are a group of cyclic hydrophobic peptides consisting of eight to ten amino acids with a molecular weight in the range of 950–2300 Da. Flax oil and seeds contain from 0.1 to 0.3% cyclopeptides, which can exhibit antioxidant, anti-inflammatory, immunosuppressive, antihypertensive and antitumor activity. The aim of this review was to systematize and summarize the available literature data on methods of extraction, separation and identification of cyclopeptides from flaxseed oil. It was found that the main methods for obtaining cyclopeptides are solid-liquid, liquid-liquid or solid-phase extraction. Commonly used solvents include methanol, hexane, ethyl acetate, dichloromethane, acetonitrile and deionized water. Preparative flash chromatography on silica gel or polymer adsorbents is used to purify and concentrate cyclopeptides, and high-performance liquid chromatography (HPLC) is used to obtain individual standards. The most commonly used stationary phases are non-polar modified sorbents — octadecyl (C18) and phenylhexyl functional groups. Identification is carried out using instrumental methods of analysis: IR spectroscopy, NMR, HPLC with a diode array detector (HPLC-PDA/DAD), high-resolution tandem mass spectrometry with electrospray ionization (ESI-HR-MS/MS). For the qualitative and quantitative determination of cyclopeptides, the HPLC with a diode array detector at a wavelength of 214 nm is sufficient. In turn, mass spectral methods, including tandem mass spectrometry, make it possible to confirm the qualitative composition and establish the amino acid sequence of cyclic peptides.

Overview of instrumental methods used in the field of food analysis

The emergence of new technologies for food production and current trends in the use of alternative sources of raw materials require improved approaches to the analysis of the chemical composition of food products. In the course of planning work to carry out analytical research, a number of challenges arise: identification of the research objects themselves; selection of research methods and tools; ensuring the representativeness and validity of the results obtained. When choosing and justifying research methods, it is necessary to rely on a large number of factors, which include both confirmation of the actual composition of food products, including the determination of unintentionally present substances, and the reliability of the data obtained, taking into account the selected instrumental methods. The purpose of this work is to generalize and systematize the essence and characteristics of the main methods of food analysis and evaluate existing approaches to the justification and application of instrumental methods of analysis in relation to new types of food products. This paper reviews the literature on instrumental research methods used to obtain values in the most representative international databases on the composition of food products (FAO/INFOODS Food Composition Databases, USDA NDL, Fineli, Frida), as well as databases of the chemical composition of food products in Russia, Japan and Australia. To search for descriptions and features of the use of analytical equipment and analytical methods, electronic library systems Web of Science, Scopus, Elibrary, ResearchGate, Google Scholar, Microsoft Academic, Science Direct were used. This review highlights the role of various research methods: photometric and electrophoretic, titrimetric, extraction, chromatographic, spectroscopic, immunoenzymatic, as well as those based on the polymerase chain reaction and the use of nuclear magnetic resonance, lateral flow and electropheresis.

Immunochromatographic food control tools: New developments and practical prospects

In the modern food production technologies, the tools and means of simple and rapid testing raw materials, intermediate products and the final ready-to-consume food products are in high demand. This monitoring allows determining the content of toxic and pathogenic contaminants and confirms the compliance of the objects being tested with the established regulatory requirements. Mobile tests tools and means (so called test systems) provide the opportunity of wide range monitoring without involving the specialized laboratories and highly qualified specialists. Thus, test systems for detection of toxic and pathogenic contaminants serve as the useful addition to confirming instrumental analytical methods. An actively developing approach for this field testing is the using of immunochromatographic test strips, in which strips all the necessary reagents are applied to the membrane components of the analytical system. Contact of the test strip with the sample being tested, initiates all further interactions and generates the recordable or visually assessable optical signal. The market of test systems based on immunochromatographic analysis is constantly growing, thus offering the permanently widening choice of solutions. However, in recent years there has been a real boom of new developments in immunochromatography field, thus offering various options for highly sensitive and information capacitive analytical systems. This study systematizes these developments and provides their comparative assessment in terms of prospects for their technological implementation and practical application in the coming years. The opportunities of designing the antibodies and alternative receptor molecules for controlling the affinity and the selectivity of recognition of the compounds being monitored are considered. The advantages and limitations of the new nanodispersed markers and non-optical methods for their registration in immunochromatography are discussed. The methods for quantitative assessment of the contaminants content via immunochromatography are characterized. The developed design options of the test systems for multiplex control — simultaneous detection of several compounds — are presented. Examples of integration of immunochromatographic tests with the systems of automatic registration, processing, transfer, storage and analysis of results of numerous tests are represented.

Occurrence of contamination and the reduction and transfer of Alternaria toxins in apples during processing

Alternaria spp. infect and accumulate various toxic secondary metabolites in apples, posing a threat to human and animal health. This study established instrumental analytical methods for six Alternaria toxins, systematically investigated the contamination levels of Alternaria toxins in apples and their reduction and transfer patterns during processing. Apples exhibited heightened disease susceptibility at 25°C and produced 6 Alternaria toxins in different degrees, while Alternaria toxins were not observed within 4cm of the symptomatic site. The transfer rate to pomace after juice extraction was higher than that in juice, with transfer rates ranging from 51.9 to 62.75% for pomace and from 21.44 to 35.79% for juice. Canning reduced Alternaria toxins in pulp, with the highest reduction rate of 93.3% for tenuazonic acid. A total of four Alternaria toxins were detected in the testing of market samples of apple products, and none of the assessed total daily exposure doses of toxins ingested by humans through apple products exceeded the daily intake level recommended by the EFSA. The evaluation results indicate that the risk of dietary exposure of humans is relatively low through apple products. These findings provide valuable data and theoretical support for ensuring food safety in apples.

Female BALB/c mice prefer the odor of mates producing fewer progeny

Mate choice is the very important part of sexual selection. It is known that free mate choice is to provide the most viable offspring are born. Researches on different animal species found that viability from introduction to sexual maturity is significantly higher in individuals born in crossbreeding in accordance with free behavioral mate choice, compared to that in crossbreeding against the mate choice. Making the choice, the female may rely on visual, vocal or olfactory signals of male. Most of experiments evaluating sexual choice allowing interactions with the animal, making it impossible to determine the specific contribution of each separate signal. Odor play a crucial role in intraspecific communication in rodents. Individuals are able to recognize sex, reproductive status, genotype, and diet and health condition conspecifics by odor. However, very few articles unite olfactory signals from the male to information about his paternal effects. In our research, we mated a male with two females. The number of live embryos, their weight and the weight of fetal placentas evaluated reproductive success of males. Naive females in olfactory tests then evaluated the volatile urine fraction of the males. Male urine samples were also analyzed using chromatography-mass spectrometry analysis. In result, the naive BALB/c females prefer males with low number of fetus in the litter compared to males with high number of fetus in the litter. Instrumental method of analysis approved the opportunity to differentiate between the groups of males. Other pregnancy parameters did not affect naive females’ preference for male urine samples.

Application of ZrO2 and Ni modified carbon nanotube composites as bifunctional water electrolysis catalysts

Application of ZrO2 and Ni modified carbon nanotube composites as bifunctional water electrolysis catalysts

Impact of ripening periods on the key volatile compounds of Cheddar cheese evaluated by sensory evaluation, instrumental analysis and chemometrics method

Impact of ripening periods on the key volatile compounds of Cheddar cheese evaluated by sensory evaluation, instrumental analysis and chemometrics method

Evaluating the detection of barbiturates in dried blood spots: A comparative analysis using gas chromatography-mass spectrometry, gas chromatography-tandem mass spectrometry, and liquid chromatography-tandem mass spectrometry with different extraction methods

Rapid and accurate characterization and quantitation of blood barbiturates and their combination drugs are very important for the clinical treatment of acute barbiturate poisoning. A comparison of dried blood spot (DBS) and traditional liquid-liquid extraction (LLE) in the pre-treatment stage, as well as a comparison of gas chromatography-mass spectrometry (GC-MS), gas chromatography-tandem mass spectrometry (GC-MS/MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) as instrumental analysis methods, revealed differences in the analysis results of barbiturates and their combination drugs under different conditions. Based on these findings, we introduce a DBS-GC-MS/MS method. The developed and validated method showed good selectivity, sensitivity (LOD: 0.1 μg mL-1, LOQ: 0.2 μg mL-1), linearity (R2>0.9992), trueness (<15 %, except for carbamazepine, at 29.4 %), and precision (<15 %). Recovery was also good for most target compounds, but significant matrix effects were evident. Compared with the LLE method, the DBS method has the benefits of easy sample collection, storage, and transport, as well as simple pre-treatment and reduced reagent and energy consumption. Compared to LC-MS/MS, GC-MS/MS requires no switching between positive and negative ion modes and uses the MRM detection mode, meaning that more information about the sample compounds can be obtained in less analysis time. Using actual sample analysis, we have demonstrated the advantages of the DBS-GC-MS/MS method for the qualitative and quantitative analysis of barbiturates and poisoning events due to combinations of these drugs. Comparison of the three instruments and the two treatment methods revealed their analysis characteristics. From the perspective of practical application, the broad practical value and advantages of DBS should be embraced in more applications, and future analytical laboratory development should continue to recognize GC-MS/MS as a useful supplement to LC-MS/MS.

Dissolution‐Recrystallization: A Novel Mechanism for Fluorochromic Detection of Th4+ Using Color‐Tunable Luminescent Metal–Organic Frameworks

AbstractThorium, a predominant actinide in the Earth's crust, presents significant environmental and health risks due to its radioactive nature. These risks are particularly pronounced during the mining and processing of monazite for rare earth elements (REEs), which contain substantial thorium concentrations. Current instrumental analysis methods for thorium, offer high accuracy but require laborious sample preparations and expensive instruments, making them unsuitable for on‐site analysis. Herein, we present a class of color‐tunable luminescent lanthanide‐based metal–organic frameworks (Ln‐MOFs) as fluorochromic sensors for Th4+ cations. Utilizing a heterobimetallic Eu3+/Tb3+ doping strategy, the luminescence colors of EuxTb1‐x‐BDC‐OH can be finely tuned from red, to orange, and to green. More intriguingly, the higher Lewis acidity of Th4+ facilitates the transformation of EuxTb1‐x‐BDC‐OH into a UiO‐type Th‐MOF via a dissolution‐recrystallization mechanism. This process results in a gradual reduction of characteristic Ln3+ emissions and the emergence of blue color ligand‐based fluorescence, thereby leading to selective fluorochromic responses with increasing Th4+ concentrations and enabling visible detection of Th4+ cations. Additionally, a custom‐built portable optoelectronic device is fabricated, which directly converts luminescence colors into red‐green‐blue (RGB) values. This device enables easy quantification of Th4+ concentrations without the need for complex instrumentation.

Dissolution-Recrystallization: A Novel Mechanism for Fluorochromic Detection of Th4+ Using Color-Tunable Luminescent Metal-Organic Frameworks.

Thorium, a predominant actinide in the Earth's crust, presents significant environmental and health risks due to its radioactive nature. These risks are particularly pronounced during the mining and processing of monazite for rare earth elements (REEs), which contain substantial thorium concentrations. Current instrumental analysis methods for thorium, offer high accuracy but require laborious sample preparations and expensive instruments, making them unsuitable for on-site analysis. Herein, we present a class of color-tunable luminescent lanthanide-based metal-organic frameworks (Ln-MOFs) as fluorochromic sensors for Th4+ cations. Utilizing a heterobimetallic Eu3+/Tb3+ doping strategy, the luminescence colors of EuxTb1-x-BDC-OH can be finely tuned from red, to orange, and to green. More intriguingly, the higher Lewis acidity of Th4+ facilitates the transformation of EuxTb1-x-BDC-OH into a UiO-type Th-MOF via a dissolution-recrystallization mechanism. This process results in a gradual reduction of characteristic Ln3+ emissions and the emergence of blue color ligand-based fluorescence, thereby leading to selective fluorochromic responses with increasing Th4+ concentrations and enabling visible detection of Th4+ cations. Additionally, a custom-built portable optoelectronic device is fabricated, which directly converts luminescence colors into red-green-blue (RGB) values. This device enables easy quantification of Th4+ concentrations without the need for complex instrumentation.

Detection methods for antibiotics in wastewater: a review.

Antibiotics are widely used as fungicides because of their antibacterial and bactericidal effects. However, it is necessary to control their dosage. If the amount of antbiotics is too much, it cannot be completely metabolized and absorbed, will pollute the environment, and have a great impact on human health. Many antibiotics usually left in factory or aquaculture wastewater pollute the environment, so it is vital to detect the content of antibiotics in wastewater. This article summarizes several common methods of antibiotic detection and pretreatment steps. The detection methods of antibiotics in wastewater mainly include immunoassay, instrumental analysis method, and sensor. Studies have shown that immunoassay can detect deficient concentrations of antibiotics, but it is affected by external factors leading to errors. The detection speed of the instrumental analysis method is fast, but the repeatability is poor, the price is high, and the operation is complicated. The sensor is a method that is currently increasingly studied, including electrochemical sensors, optical sensors, biosensors, photoelectrochemical sensors, and surface plasmon resonance sensors. It has the advantages of fast detection speed, high accuracy, and strong sensitivity. However, the reproducibility and stability of the sensor are poor. At present, there is no method that can comprehensively integrate the advantages. This paper aims to review the enrichment and detection methods of antibiotics in wastewater from 2020 to the present. It also aims to provide some ideas for future research directions in this field.

Generation of specific aptamers for constructing CoOOH nanozymes-based colorimetric aptasensor for detection of fleroxacin in milk

Generation of specific aptamers for constructing CoOOH nanozymes-based colorimetric aptasensor for detection of fleroxacin in milk

Methods for analysis and monitoring of heavy metals in the environment

This review surveys capabilities of modern instrumental analytical methods that provide low limits of detection for a wide range of elements. Some elements have their own toxic forms, so their identification is of particular importance. Researchers pay increased attention to the content of cadmium and lead due to their high immediate and delayed toxicity.It should be noted that those instrumental methods of analysis that are used to determine these important ecotoxicants are often used to determine other heavy metals. The following objects of analysis are considered – natural, river, sea waters; brines; bottom sediments; plants, including medicinal ones; precipitation; soil; as well as whole blood and blood serum; hair and animal fur; animal organs and tissues. Special attention is paid to eliminating matrix influences and reducing limits of detection using concentration procedures. Various types of extraction, sorption, precipitation, chemical transformation (hydride generation, etc.) are considered and applied to biological and environmental objects.The capabilities of modern instrumental methods of analysis for the determination of heavy metals and important ecotoxicants are considered and compared – single‐element (ETAAS, IVA, etc.) and multi‐element (ICP AES, ICP MS, XRF, etc.). The data is structured in the form of a table, which shows specific analysis methods, indicating the method of sample preparation and/or features of instrumental determination, a list of elements to be determined and the limits of detection.

Development and application of a comprehensive measurement equation for the direct comparator standardization method of Instrumental Neutron Activation Analysis

Development and application of a comprehensive measurement equation for the direct comparator standardization method of Instrumental Neutron Activation Analysis

Synthesis of phenyl esters 2-oxo-2H-1-benzopyran-3-carboxylic acids as promising antimicrobial agents

Corresponding chloroanhydrides were synthesized by the interaction of coumarin-3-carboxylic and 6-methoxycoumarin-3-carboxylic acids with an excess of thionyl chloride under heating. Due to the quantitative yields and the formation of volatile products within the reaction, chloroanhydrides were used without additional purification in acylation of substituted phenols for the synthesis of phenyl esters of 2-oxo-2H-1-benzopyran-3-carboxylic acids. The reaction was carried out by heating in anhydrous dioxane environment in the presence of pyridine as a catalyst. The structure of the obtained esters was proven using instrumental methods of analysis. The antimicrobial and antifungal activity of the synthesized compounds was studied in vitro by the method of two-fold serial dilutions in liquid and solid nutrient environments. Antimicrobial activity screening showed that the synthesized esters have a moderate activity against gram-negative microorganisms and C. albicans. It was found that the 4-methyl-2-chlorophenyl ester of coumarin-3-carboxylic acid had the greatest bacteriostatic activity against Ps. aeruginosa and Pr. vulgaris, 2-chlorophenyl ester of coumarin-3-carboxylic acid against Ps. aeruginosa and E. coli, coumarin-3-carboxylic acid 4-hydroxyphenyl ester against Pr. vulgaris, and coumarin-3-carboxylic acid 2-isopropyl-5-methylphenyl ester against E. coli at a concentration of 31.25 g/ml. 2-Chlorophenyl ester also showed a bactericidal effect against E. coli. The synthesized compounds also have an antifungal effect against the C. albicans. strain used in the experiment. Thus, 4-methyl-2-chlorophenyl ester, 4-fluorophenyl ester, 4-hydroxyphenyl ester, 2,3-dihydroxyphenyl ester, 2-isopropyl-5-methylphenyl ester of coumarin-3-carboxylic acid are active at a concentration of 31.25 g/ml.

SAFETY ASSESSMENT OF A RANGE OF PLANT METABOLIC TOVES – ADVANCED GEROPROTECTORS IN VIVO RESEARCH

Background. Ageing is associated with progressive impairment of physical functions and increased risk of age-related chronic diseases. Polyphenols such as mangiferin, quercetin and trans-cinnamic acid are also used for the treatment and prevention of chronic diseases. Toxicological research was important in biomedical research, as some high-dose extracted polyphenols could contribute to the development and progression of cancer and other diseases. This study is aimed at assessing toxicological safety of previously isolated phenolic compounds from plants of the Siberian Federal District. Materials and methods. Rodents have been chosen as the model organism. The study of mutagenic and antimutagenic properties was carried out using a micronuclear test of bone marrow cell. Cytotoxicity in vitro was assessed using the MTT test. Results. Polyphenols studied at doses of 50.0 and 100.0 mg/kg are not mutagenic. Mangiferri and quercetin at a dose of 50.0 mg/kg do not have anti-thagenic effects. In this case, trans-cinnamic acid in the same concentration has antimutagenic effect. Mangiferin in concentrates from 4.2 to 16.7 ug/ml has a cytotoxic effect. Trans-cinnamic acid is not cytotoxic (at 2.1-33.3 ug/ml). Quercetin has a cytotoxic effect in concentrations of 0.21 and 1.67 ug/ml, and at concentrations of 3.33 ug/ml increases the viability of splenocytes, presumably due to antioxidant properties. Conclusion. Safety or low toxicity is a decisive advantage of the continued use of polyphenols as advanced heroic flow agents. Further study of biologically active substances (mangiferin, quercetin and trans-cinnamic acid) has been found to be ce-woody. The work was carried out by the state task on the topic «Development of biologically active additives consisting of metabolites of plant objects in vitro to protect the population from premature aging» (project FZSR-2024-0008) using the equipment of Center of Collective Use «Instrumental methods of analysis in the field of applied biotechnology» on the basis of KemSU.

Association between gut microbiota and hepatocellular carcinoma and biliary tract cancer: A mendelian randomization study

BACKGROUND An increasing number of studies have begun to discuss the relationship between gut microbiota and diseases, yet there is currently a lack of corresponding articles describing the association between gut microbiota and hepatocellular carcinoma (HCC) and biliary tract cancer (BTC). This study aims to explore the relationship between them using Mendelian randomization (MR) analysis method. AIM To assess the relationship between gut microbiota and HCC and BTC. METHODS We obtained Genome-wide association study (GWAS) data for the gut microbiome from the intestinal microbiota genomic library (MiBioGen, https://mibiogen.gcc.rug.nl/ ). Additionally, we accessed data pertaining to HCC and BTC from the IEU open GWAS platform (https://gwas.mrcieu.ac.uk/ ). Our analysis employed fundamental instrumental variable analysis methods, including inverse-variance weighted, MR and Egger. To ensure the dependability of the results, we subjected the results to tests for multiple biases and heterogeneity. RESULTS During our investigation, we discovered 11 gut microbiota linked to an increased risk to BTC and HCC. The former included the genus Eubacterium hallii group (P = 0.017), Candidatus Soleaferrea (P = 0.034), Flavonifractor (P = 0.021), Lachnospiraceae FCS020 (P = 0.034), the order Victivallales (P = 0.018), and the class Lentisphaeria (P = 0.0.18). The latter included the genus Desulfovibrio (P = 0.042), Oscillibacter (P = 0.023), the family Coriobacteriaceae (P = 0.048), the order Coriobacteriales (P = 0.048), and the class Coriobacteriia (P = 0.048). Furthermore, in BTC, we observed 2 protective gut microbiota namely the genus Dorea (P = 0.041) and Lachnospiraceae ND3007 group (P = 0.045). All results showed no evidence of multiplicity or heterogeneity. CONCLUSION This study explores a causal link between gut microbiota and HCC and BTC. These insights may enhance the mechanistic knowledge of microbiota-related HCC and BTC pathways, potentially informing therapeutic strategies.

A methodological study of the hardness of cooked Chinese noodles based on the texture analyzer

Abstract A generic instrumental analysis method, matched with sensory evaluation, for the hardness test of Chinese dried noodles after cooking, was established in this study. The cut and compression mode was compared at first, followed by an analysis of the hardness response curve obtained from cut code with three-stage. Then the reliability of this method was verified with 20 commercially available noodles. Results showed that the hardness difference between the two samples obtained using compression mode was only 0.087 %. In contrast, a 6.34 % difference was obtained using cut mode with the hardness value of 296.12 ± 6.10 g and 278.47 ± 12.22 g, respectively. Three parameters, including initial hardness (F 1), material hardness (K), and final hardness (F 2), were extracted from the shear curve to characterize the hardness of cooked noodles. A ranking difference of F 1, F 2, and K between 20 commercially available noodles was found, which illustrated various characteristics of cooked noodles. In summary, the cut mode was more suitable for determining the hardness of cooked noodles; F 1, F 2, and K, obtained from a three-stage analytical method for the inner and outer hardness of cooked noodles characterization, had excellent performance to match with sensory evaluation.

Phenotyping Wheat Kernel Symmetry as a Consequence of Different Agronomic Practices

The use of instrumental methods of analysis in the assessment of indices that record changes in symmetry in the structure of grains to evaluate the quality of durum and soft wheat grain is currently considered a search tool that will allow us to obtain previously unavailable data by finding correlations associated with differences in the shape and ratio of starch granules in conditionally symmetrical and asymmetrical wheat fruits (kernels) formed in different field conditions and with different genotypes. Indicators that had previously shown their effectiveness were used to analyze the obviously complex unique material obtained as a result of growing under critically unique sowing conditions in 2022, which affected the stability of grain development and filling. For the evaluation, a typical agronomic comparative experiment was chosen, which was used to evaluate the soil tillage practices (fallow, non-moldboard loosening, and plowing) and sowing dates (early and after excessive rainfalls), which made it possible to analyze a wider range of factors influencing the studied indices. The soil tillage methods were found to affect the uniformity of kernel fullness and their symmetry, and the sowing dates did not lead to significant differences. This study presents detailed changes in the shape of the middle cut of a wheat kernel, associated with assessing the efficiency of kernel filling and the symmetrical distribution of storage substances under the influence of external and internal physical factors that affect the formation of the wheat kernel. The data obtained may be of interest to breeders and developers of predictive phenotyping programs for cereal grain and seeds of other crops, as well as plant physiologists.

Extraction Optimization, Structure Analysis and Antioxidant Activity of Polysaccharide from Sanghuangporus baumii

Introduction: In this study, we optimized the extraction process, analyzed the structure and assessed the antioxidant activity of Sanghuangporus baumii polysaccharide (SBP). The present results provide important information for the Sanghuangporus baumii polysaccharides in potential natural antioxidant effects. Background: The extraction and structural analysis of polysaccharides from Sanghuangporus has gained significant attention in the fields of chemistry, medicine, and life sciences. There is great significance in maximizing the extraction of polysaccharides from Sanghuangporus and developing their potential products in a scientific and rational manner. Objective: The study was designed to establish an efficient and practical extraction process for SBP, and then investigated the structure and the antioxidant activity. Methods: The Response Surface Methodology (RSM) based on Box-Behnken design was used to explore the ultrasound-assisted extraction of SBP, and the structure of SBP was studied by ultraviolet spectroscopy, infrared spectroscopy and other instrumental analysis methods. The total antioxidant capacity of SBP was studied by the Ferric Reducing Ability of Plasma (FRAP) method, and the scavenging capacity of ABTS+·, DPPH· and OH· was carried out as the index to investigate its antioxidant activity in vitro. Results: The statistical analysis results showed that the optimal conditions for extracting SBP were an ultrasound time of 20.74 min, ultrasound power of 268.40 W and material-liquid ratio of 1:25.71. Under optimal conditions, the experimental yield of SBP was 3.36 ± 0.01%. The RSM optimization process was applied to the experiment of complex enzyme extraction of SBP, and the yield increased to 4.72 ± 0.03%. Structural analysis showed that SBP mainly consisted of glucose, a small amount of mannose and galactose, and the molecular weight distribution was uneven, mainly concentrating in the three parts of 24.5,6.4,2.5 kDa. Moreover, SBP exhibited dose-dependent and strong reducing power and radical scavenging activity. For DPPH·, ABTS+· and OH· radical scavenging assays, IC50 values were 1.505 ± 0.050 mg/ml, 0.065 ± 0.036 mg/ml and 0.442 ± 0.007 mg/ml, respectively. Conclusion: In the present study, a β-linked heteroglucan (SBP) was extracted using the optimized process combining enzymes from the fruiting bodies of Sanghuangporus baumii. SBP exhibited effective and dose-dependent antioxidant activities. Our findings were of great value in terms of developing polysaccharides with potential natural antioxidants.