Music students are more likely to get Musculoskeletal Disorders (MSDs), fatigue, and pain. However, limited studies compared MSDs, pain and fatigue between different types of musical instrument players. This study compared MSDs, pain, and fatigue among music students who play wind and string instruments. A cross-sectional study was conducted with a group of music students in one of the universities in Malaysia. No statistical differences in pain and fatigue were found, but more wind players experience MSD at the wrist and hand than string players. Therefore, students who play string instruments should be more watchful with MSD symptoms. Keywords: Musical instruments, pain, fatigue, musculoskeletal disorder eISSN: 2398-4287 © 2022. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians/Africans/Arabians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia. DOI: https://doi.org/10.21834/ebpj.v7i20.3457