Inspection Of Carcasses Research Articles

Veterinary-Sanitary Inspection of the Upland Game Meat from the Yakutian Habitat

This article presents the results of the veterinary-sanitary inspection and research of upland game carcasses. During the primary inspection, we paid special attention to the presence of abnormal changes typical of infectious and invasive diseases, based on the standard rules for inspecting the meat of domestic fowl. We also took into consideration the nature of injuries, dehematizing levels, product quality, and freshness. The preservation of the upland game meat depends not only on the rapid removal of intestines from carcasses but also on the type and area of injuries, and the level of dehematization. The study matter for this research is represented by the carcasses of upland game, namely their muscle tissues. During the research, we used organoleptic, physical, chemical, bacteriological, pathomorphological, and mathematic methods. During the organoleptic tests, the highest appraisal of upland game products on a 9-point scale was within the range of 8.4-9, which complies with the standards. The content of amino-ammonia nitrogen was 0.78±0.01. The primary protein breakdown product test showed that the fresh meat produced clear stock without flaking. The fat acidity value test showed an increase from 0.8±0.01 to 3.9±0.01 mg/KOH; the fat peroxide value increased from 0.01±0.01 to 0.74±0.5% J. The pH value varied from 5.99±1.79 for fresh meat to 7.70±0.01 for stale upland game meat. The microbiological tests did not find any pathogenic germs, including salmonella and Listeriamonocytogenes bacteria.

Intra-vitam Diagnosis of Tuberculosis in Pigs: Concordance Between Interferon-Gamma Release Assay and Comparative Tuberculin Skin Test.

The role of pigs in the maintenance of bovine tuberculosis caused by Mycobacterium bovis has been demonstrated in many settings; however, the current control programs usually do not state any intra-vitam diagnostic procedure in this species, as for the cattle. Carcass inspection has shown to be insufficient to detect infection in swine; thus, the assessment of intradermal tuberculin test and interferon-gamma release assay (IGRA) in this species is mandatory. The current study compares the performances of the single intradermal comparative cervical tuberculin (SICCT) test and IGRA. A total of 628 Nebrodi Black pigs raised in free-roaming farms were subjected to the two tests simultaneously. Besides, 124 animals underwent postmortem examination for the detection of tuberculous lesions and isolation of mycobacteria from target organs. The two tests showed a concordance of 94.42% with a Cohen's k coefficient of 0.786 and McNemar chi-square of 4.83 (P = 0.03). Slightly lower levels of concordance (90.32%) between SICCT and IGRA were obtained in the group of 124 animals, with a Cohen's k = 0.797 and McNemar chi-squared value of 0.69 with a non-significant P = 0.41. Moreover, the results showed how IGRA tends to result positive in higher rates, mostly when non-tuberculous mycobacteria (NTM) were isolated, suggesting a possible impairment of specificity in the event of coinfections in the swine. In conclusion, the results obtained support the possibility of the strategic use of IGRA or SICCT in combination or alternatively one to the other, particularly IGRA which showed lower specificity but has evident advantages over SICCT.

Classification of Vertebral Osteomyelitis and Associated Judgment Applied during Post-Mortem Inspection of Swine Carcasses in Portugal.

Vertebral osteomyelitis (VO) it is often a suppurative lesion that, in Portugal, represents the main cause of total condemnation of slaughtered finishing pigs. Based on the EU Meat Inspection legislation, meat from generalized VO cases presenting signs of pyemia should be declared unfit for human consumption. For that reason, the main objective of this study is to establish a classification scheme to differentiate between localized and generalized VO cases using macroscopic findings and validate it based on the presence of pyemia. To assist in, a combination of macroscopic characteristics of gross lesions (e.g., presence of pyaemia-related lesions (PRL), acute/chronic characteristics of VO) was used to create a classification scheme to differentiate between localized and generalized VO cases. The scheme was applied to 40 VO cases that had been totally condemned in an undifferentiated way. In those 40 cases, histopathological analysis was used to validate acute/chronic macro-criteria, and microbiological analysis was performed to identify the pyemia cases. From the 40 selected VO cases, 20 were macroscopically classified as chronic and 20 as acute. Cohen’s kappa coefficient (κ = 0.80; p < 0.001), revealed a substantial agreement between macroscopic and histopathology classification. Microbiological analyses identified 13 pyemia cases (13/40; 32.5%). Among those, 12 were macroscopically classified as acute, this association being highly significant (p < 0.001). By using the proposed VO classification scheme, 14 possible cases out of 40 could have been spared from total condemnation. This scheme can be used to harmonize the classification of VO and meat inspection decisions in Portuguese abattoirs. The output would lead to avoidance of unnecessary carcasses condemnation (food waste/economic losses), under an evidence-based approach, without compromising food safety and public health as demanded by the EU Meat Inspection legislation.

Trichinella spp. in wild boar (Sus scrofa) populations in Croatia during an eight-year study (2010–2017)

Wild animals represent a constant source of Trichinella spp. infections for domestic animals and humans. To date, four species of Trichinella have been isolated in wild boar populations in Europe: T. pseudospiralis, T. spiralis, T. britovi and T. nativa, in addition to several mixed infection types and one hybrid formation between T. britovi and T. spiralis. Meanwhile, insufficiently thermally processed wild boar meat has been reported to be a source of trichinellosis in humans in several European countries. In Croatia, there have been no reported or proven cases of trichinellosis caused by wild boar meat consumption. The aim of this study was to obtain data on the prevalence of Trichinella species present in Croatia and to anticipated the potential risk of infection for humans in specific Croatian regions based on information obtained over an eight-year surveillance period. A veterinary inspection of wild boar carcasses for Trichinella larvae in Croatia has been mandatory since 1989, and the artificial digestion method was introduced as a compulsory test for wild boar samples in 2008. Based on the official data submitted to the Ministry of Agriculture, Directorate of Veterinary Services, in the period 2010–2017, 303 of 183,184 (0.17%) wild boar meat samples tested positive for Trichinella spp. Infected wild boar were found in 18 of 21 counties. Of these positive samples, 85 were submitted by the authorised veterinary inspectors to the National Reference Laboratory for further examination. The intensity of infection in muscle samples was 0.04–152.66 (mean: 23,37) larvae per gram, and Trichinella species were identified as T. spiralis, T. britovi, T. pseudospiralis and T. spiralis + T. britovi. Genetic analysis of T. pseudospiralis isolates demonstrated their belonging to the Palaearctic population.

ПОКАЗАТЕЛИ ИДЕНТИФИКАЦИИ PSE-ПОРОКА И МИОПАТИИ ИНФЕКЦИОННОЙ И ИНВАЗИОННОЙ ЭТИОЛОГИИ

The materials on identification in broiler meat the features of PSE- defect and myopathy are shown, associated by causative agents of various infectious and infective diseases. Specific differences in pathomorphology symptoms of PSE-meat and myopathy of infectious origin are established. Symptoms of PSE- defect in broiler meat emerge more often (11,7 – 21,7%), than signs of degenerative changes from exposure of infectious disease agents and muscle invasion(4,19-7,61 %). It was established that changes in muscles depend on degree of development PSE-defect. That’s why during veterinary –sanitary inspection of organs and broiler carcasses we can determine not only features of PSE-defect, but degree of their development . During veterinary- sanitary inspection of poultry meat, four development stages of PSE-defect can be determined: extreme, florid, moderate, ill-defined. The criteria of such meat assessment are best attested рН value, muscle color, water capacity, muscle consistency on a cut and other deviations in organo-leptic attribute. The most reasonable procedure of poultry meat processing with signs of PSE-defect and myopathy of infectious aetiology. It is recommended in all cases of muscle fiber defeat as a result of PSE- defect or infectious and invasive diseases, broiler carcasses, to consider “limitedly suitable” and undergo laboratory research. In case of weak localized muscular tissue affect of carcasses it is wise to aim for boiling. In case of infectious and invasive disease, associated with evident muscle disorder, carcasses need to aim for utility waste. Meat of forcedly killed poultry after thermal antisepsis can be used for production of cooked sausages and tinned meat.

A qualitative risk assessment approach to microbial foodborne hazards in Brazilian intensive pork production: A step towards risk prioritization

The application of biosecurity measures in pig farms has prompted a shift in epidemiological reality, altering the risk profile attributed to the consumption of pork. The inspection of pig carcasses in Brazil is based on incisions and palpations to seek lesions mainly related to classic zoonotic diseases. We describe the use of a qualitative risk assessment from the farm to the table to characterize the risk of foodborne disease caused by biological hazards to humans due to the consumption of pork. The model was adapted from the Codex Alimentarius and comprises four steps: (I) hazard identification, (II) hazard characterization, (III) exposure assessment, and (IV) risk characterization. The exposure assessment step considers the release of hazards at a pre-harvest level (i.e., swine in herds) and the amplification or reduction of the release during the procedure to obtain pork from a carcass. Risk characterization is classified into five levels, from very low to very high. No hazard had very high risk, Salmonella sp. [nontyphoidal] had high risk, no hazard had moderate risk, seven hazards had low risk, and other hazards had very low risk. When the initial release was increased, the risk for hazards with higher adverse effects (e.g. C. botulinum, B. suis, and E. coli [STEC]) increased to very high or high. The uncertainty was very high for seven hazards and very low for three. These findings can help the Brazilian Ministry of Agriculture discuss changes in the routine inspection of the carcasses of pigs raised in industrial systems in Brazil.

Automated Classification for Visual-Only Postmortem Inspection of Porcine Pathology

Several advantages would arise from the automated detection of pathologies of pig carcasses, including avoidance of the inherent risks of subjectivity and variability between human observers. Here, we develop a novel automated classification of two porcine offal pathologies at abattoir: a focal, localized pathology of the liver and a diffuse pathology of the heart, as cases in point. We develop a pattern recognition system based on machine learning to identify those organs that exhibit signs of the pathology of interest. Specifically, deep neural networks are trained to produce probability heat maps, highlighting regions on the surface of an organ potentially affected by a given condition. A final classification stage then decides whether a given organ is affected by the condition in question based on statistics computed from the heat map. We compare outcomes of automated classification with classification by expert pathologists. Results show the classification of liver and heart pathologies in agreement with an expert at levels comparable to, or exceeding, interexpert agreement. A system using methods such as those presented here has potential to overcome the limitations of human-based abattoir inspection, especially if this is based on visual-only inspection, and ultimately to provide a new gold standard for pathology. Note to Practitioners —The motivation for this article reflects the current requirement for visual-only inspection of livestock carcasses at slaughter houses and the need to provide a gold standard for recognition of carcass pathologies. Visual-only inspection is motivated by the need to reduce cross contamination between carcasses by manual palpation, but this leads to substantial variability in detection accuracy both within and between inspectors. This has significant public health implications. Here we present a system that comprises hardware to capture images of pig offal and software to analyze those images and identify cases of liver milk spots and hearts affected by pericarditis. It can classify high proportions of offal with accuracy comparable to that of veterinarians with extensive experience in pig pathology, thus demonstrating the potential to overcome the limitations of human-based abattoir inspection (especially if it is visual-only) and ultimately to provide a new gold standard. Our work is the first to address the automation of pig offal inspection, thus shedding light on the challenges associated with both appropriate image capture and successful image analysis, such as the need to cope with wide variations in the appearance of both normal and diseased organs, as well as different types of lesions and their impact on how much effort is required from experts in order to produce data needed to train the system. Future directions of work should include extending the system to identify more pathologies and implementing a real-time system to cope with production line speed.

Bovine tuberculosis: diagnosis in dairy cattle through the association of analyzes

ABSTRACT: Tuberculosis is a chronic anthropozoonosis of worldwide occurrence, caused by the bacterium Mycobacterium tuberculosis and its variants. In Brazil, the National Program for the Control and Eradication of Brucellosis and Tuberculosis in cattle, is responsible for diagnosing and the correctly allocate positive animals, but there is still a lack of definitive diagnosis of the disease. This study described the use of five diagnostic tools that can be used, preferably together, for the confirmation of suspected cases. These tools included the clinical examination comparative cervical tuberculin test, macroscopic findings during the slaughtering and histopathology of the damaged tissues followed by histochemistry. We evaluated a total of 211 dairy cattle, where 15.1% (32/211) had classic clinical signs of bovine tuberculosis, 74 (35%) showed reactivity in the comparative cervical tuberculin test. Of the total number of animals, 141 (66.8%) were referred for sanitary slaughter due to legal and control issues in the outbreaks of the disease. In the follow-up of slaughtering and inspection of viscera and carcasses, 74 (52.5%) had macroscopic lesions compatible with bovine tuberculosis, while 67 (47.5%) showed no visible changes. During the inspection, fragments of lymph nodes and liver and lung parenchyma were collected from five cattle with macroscopic lesions and five with no lesions. The histopathological analysis showed numerous areas of caseous necrosis with or without central calcification and granulomatous inflammatory infiltrate. In the special staining of Ziehl-Neelsen, numerous acid-fast bacilli were evidenced in all cases.

Economic Losses’ Estimation of carcass and Organ Condemnations From Slaughter Cattle in Thailand, Pará State, Brazilian Amazon

The article identifies the main pathologies found and estimates the economic losses generated in a slaughterhouse in the Thailand municipality, Pará, during the period from March 2010 to October 2014. 55,169 animals were slaughtered, with total economic losses of R$ 1,221,035.90 and ischemia was the most frequent lesion (41.86%). The most frequent condemnation organs were lungs (48.75%), kidneys (41.66%) and liver (3.61%), which produced economic loss of R$ 76,405.65 (6.26%). Tuberculosis was the main cause of carcass condemnation, responsible for R$ 872,783.64 of economic losses. The bovine tuberculosis control can begin in production, with examinations on the farm to slaughter lines, with careful carcass inspection, as advocated by the National Program for the Control and Eradication of Animal Brucellosis and Tuberculosis (PNCEBT), which increases disease control and minimizes condemnations and economic losses at slaughter.

Use of slaughter condemnation data to detect cattle health events in near real-time

Use of slaughter condemnation data to detect cattle health events in near real-time

Prevalence of Cysticercosis Bovis in Eastern Shoa of Oromia, Ethiopia

Bovine cysticercosis is an infection of cattle caused by the larval stage, Cysticercus bovis of the human intestinal cestode, Taenia saginata . It is an infection of public health significance as eating of raw or undercooked beef results in taeniasis in human population and an important cause of economic loss mainly due to condemnation and downgrading of infected carcasses. A cross sectional study was conducted during April 2016 to August 2016 to estimate the prevalence of bovine cysticercosis in cattle slaughtered at Meki municipal abattoir. Routine inspection of carcasses and viability test were amongst the methods employed during data collection. Post mortem examination of 876 slaughtered cattle showed that 269 (30.7%) were infected with T. saginata . Out of the total 447 cysts detected, 216(48.3%) were found to be viable, while 231(51.7%) were died cysts. The tongue, lung, masseter muscles, heart and triceps muscles were the main predilection sites of the cysts. Anatomical distribution based analysis of the cyst showed that highest proportions were observed in tongueas (15.5%) followed by lung (14.4%), masseter muscle 90(10.3%), heart 61(6.9%) and triceps muscle 34(3.9%). Out of the 785 male cattle examined, 237 (30.2%) had cysts of bovine cysticercosis while 32 (35.2%) of the 91 female were infected. The prevalence varied significantly (P&lt;0.05) between age groups, origin of animals and body condition score of animals, but no significant variation between sex of the animals. With respect to viable cysts, it was revealed that lung (51.1%), tongue (48.9%), heart (28.6%), masseter muscle (19.5%) and triceps muscle (14.3%) were infected in decreasing order. The current study suggests that high prevalence of T. saginata metacestodes were observed in the source of animals and therefore sufficient emphasis should be given to this problem so as to improve health, quality and quantity of bovine that may satisfy the domestic requirements of the area and the country as general. Keywords: Bovine, cysticercosis bovis, prevalence, Meki abattoir, Ethiopia DOI : 10.7176/JBAH/9-7-09 Publication date : April 30 th 2019

Second line molecular diagnosis for bovine tuberculosis to improve diagnostic schemes.

Surveillance of bovine tuberculosis (bTB) is partly based on the sanitary inspection of carcasses at the abattoir to detect bTB-like lesions which, in compliance with EU recommendations, are analysed by bacteriology and histopathology to disclose Mycobacterium bovis (or M. caprae) infection. Moreover, since 2012, a PCR method with similar sensitivity and specificity values of histopathology and bacteriology respectively is additionally employed in France, partially compensating for the weaknesses of classical diagnostic methods. We analysed a collection of bTB-like lesions from cattle presenting positive histological results albeit with negative PCR results. We present here the results of these samples, recovered from 292 animals culled between 2013 and 2016, analysed with a second line molecular diagnosis approach that consists in a combination of PCRs targeting the M. tuberculosis-M. avium complexes as well as the Mycobacterium genus and sequencing of hsp65 gene. These molecular analyses disclosed to identify the presence of non-tuberculous bacteria which could be responsible for most of these non-specific TB lesions: non tuberculous mycobacteria (24%) or Actinomycetales (56%) such as Rhodococcus equi (53%); 24% of the samples were negative. M. bovis -or any other MTBC members- was neither detected by molecular methods nor isolated in any of them at the end of the 3 months of culture. In conclusion, these results highlight the lack of specificity of histopathology and the usefulness of a first line PCR with a second line molecular diagnostic test to circumvent it. This diagnostic strategy makes it possible to reduce the number of suspect bTB cases raised at the abattoir or shortening their lock-up periods. By simplifying diagnostic schemes, the use of this tool could improve bTB surveillance and make eradication programs more efficient in the future.

Risk-based inspection as a cost-effective strategy to reduce human exposure to cysticerci of Taenia saginata in low-prevalence settings

BackgroundTaenia saginata cysticercus is the larval stage of the zoonotic parasite Taenia saginata, with a life-cycle involving both cattle and humans. The public health impact is considered low. The current surveillance system, based on post-mortem inspection of carcasses has low sensitivity and leads to considerable economic burden. Therefore, in the interests of public health and food production efficiency, this study aims to explore the potential of risk-based and cost-effective meat inspection activities for the detection and control of T. saginata cysticercus in low prevalence settings.MethodsBuilding on the findings of a study on risk factors for T. saginata cysticercus infection in cattle in Great Britain, we simulated scenarios using a stochastic scenario tree model, where animals are allocated to different risk categories based on their age, sex and movement history. These animals underwent different types of meat inspection (alternative or current) depending on their risk category. Expert elicitation was conducted to assess feasibility of scenarios and provide data for economic analysis. The cost-effectiveness of these scenarios was calculated as an incremental cost-effectiveness ratio, using the number of infected carcasses detected as the technical outcome.ResultsTargeting the high-risk population with more incisions into the heart while abandoning incisions into the masseter muscles was found to reduce the total number of inspections and cost, while simultaneously increasing the number of infected carcasses found.ConclusionsThe results suggest that, under reasonable assumptions regarding potential improvements to current inspection methods, a more efficient and sensitive meat inspection system could be used on animals categorised according to their risk of harbouring T. saginata cysticercus at slaughter. Such a system could reduce associated cost to the beef industry and lower microbial contamination of beef products, improving public health outcomes.

Analysis of the causes of the seizure and destruction of carcasses and organs in a slaughterhouse in central Italy in the 2010-2016 period.

Meat safety and hygiene are fundamental issues for producers and endusers. To achieve these goals, the inspection of carcasses and organs at slaughter is critical. The results of post-mortem inspection are the basis for planning preventive measures against consumer risks and for limiting economic losses. In this retrospective study, the causes of the seizure and destruction of carcasses and organs were analysed at a slaughterhouse in central Italy from 2010 to 2016. 436,646 slaughtered animals were taken into consideration, for a total of 61,799 seizures (73.29% pigs, 23.87% cattle, 2.77% sheep and 0.07% horses). The organs or groups of organs that most frequently showed lesions in pigs were liver (72.38%), heart (10.77%) and pluck (10.20%); in cattle, lungs (64.86%), liver (31.20%) and stomachs (11.63%); in sheep, liver (77.15%), pluck (18.70%) and lung (3.80%); in horses, liver (75.56%), kidney (68.89%) and lung (31.11%). Among the diagnoses, parasitic diseases of the liver (ascariasis and distomatosis) were especially frequent in all species, followed by pericarditis and polyserositis in pigs and diseases affecting the respiratory system in cattle. The data obtained show that postmortem inspection is of fundamental importance for limiting risks for the consumer and ensuring meat safety. It is also evident, even more than ten years after the coming into force of the so-called hygiene package regulations, that the slaughterhouse can still act as an epidemiological observatory to provide the data needed for the development of plans for the control and eradication of the most frequent diseases in the area.

Meat inspection and hygiene in a Meat Factory Cell – An alternative concept

The Meat Factory Cell (MFC) concept differs from conventional abattoirs by partly working in cell stations instead of production lines. It combines and merges elements of today's separate processes and disciplines, namely “slaughter” and “meat primal cutting”, and “disassembles” the carcass from outside-in where limbs, neck, back and loin are removed before internal organs. The aim of this work is to qualitatively assess future meat inspection and hygiene of pork carcasses in the MFC. A holistic assessment of the carcass parts is needed to interpret the significance of findings on separate parts. The MFC offers some opportunities for targeted inspection with cutting edge diagnostic technology. Improved hygiene is expected from the MFC concept due to the fact that limbs, neck and loin are removed first and are not subject to faecal contamination from intestinal content. The MFC provides opportunities for customized chilling regime for different parts, targeted decontamination or pathogen killing processing, which should contribute to safer meat products and less energy consumption. We expect that the MFC approach will potentially fulfill the principles of Codex alimentarius and will improve public health compared to conventional slaughter and meat inspection.

Lesions Consistent with Tuberculous Spondylitis in Domestic and Wild Swine and Their Potential Use as a Model for Pott Disease in Humans.

During a routine abattoir inspection of pig carcasses and control activities of hunted wildlife in 2013, 118 large white pigs Sus scrofa domesticus, 474 Nebrodi black pigs and 135 wild boars Sus scrofa scrofa were submitted to anatomopathological examination to evaluate the presence of tuberculosis-like lesions. Localized and generalized granulomatous lesions were detected with a prevalence of about 25% in large white pigs, 13% in Nebrodi black pigs and 8.15% in wild boars. Localized lesions involved mainly the submandibular lymph nodes, but when the disease was spread throughout the body, the inner organs and, also, in some cases, udders and/or bones were injured. The highest prevalence of generalized lesions (15/30) was observed in large white pigs, the only ones in which tuberculous granuloma affected also the spine. The bovine tuberculous spondylitis cases observed showed some similarities with Pott disease in humans regarding aspect and localization of lesions and age of the affected animals.Tissue samples of the positive animals were collected and submitted to bacteriological analysis, and the bone samples were also subjected to histological and immunohistochemistry analysis. M. bovis was isolated in all the analysed samples, and the granuloma encapsulation was found often incomplete indicating that the disease was in an active phase. The presence of lesions associated to tuberculous spondylitis in pigs suggests the possibility to use this animal species as model for the study of Pott disease in humans.

Моніторинг безпечності та якості продуктів забою свиней, отриманих в умовах Одеської області

In accordance with the modern requirements of the international control system (HACCP), it is necessary to abandon the control of the final product for the purpose of identifying shortcomings, but to proceed to the preventive approach of eliminating potential sources of danger at all stages of production. A step-by-step system for controlling the quality of livestock products, in particular meat, in the absence of modern technology of primary processing and quality veterinary and sanitary control, is complex. Therefore, to ensure the safety and quality of meat, it is necessary to improve and scientifically justify the methods of their control, bringing it closer to international requirements. The purpose of our research was to monitor the safety and quality of slaughter products for pigs obtained in the conditions of the Odessa region. The material of the research was reporting on veterinary medicine (Form No. 6-vet., Form No. 5- vet., Form No. 2a-vet.) In the districts of the Odessa region for 2014–2016, which was processed statistically. The analysis has established that for 2014–2016. In the investigated areas, only 28846 pigs were slaughtered, and 74.48% of the slaughter took place. It was found that according to the results of the pre-blast inspection and post-mortem veterinary examination of pig carcasses, 83 cases of diseases, echinococcosis, were detected during the reporting period. For the reporting years, 17103 carcasses of pigs were supplied to the agro-food markets of the study areas. Employees of laboratories for veterinary and sanitary examination in the markets conducted 65417 laboratory studies: all carcasses of pigs were examined organoleptically (including cooking breakdown), trichinoscopy and studies for phychosis and other parasitic diseases were performed; The determination of physicochemical parameters and bacterioscopy of mascara were carried out in individual cases; radiological and other studies were not conducted. During the veterinary and sanitary examination, 662 cases of diseases were detected in the laboratories of veterinary and sanitary examination in the markets: 65 cases of non-communicable diseases and 597 cases of invasive diseases. And draws attention to the fact that 99.83% of the identified invasive diseases was echinococcosis. Comparative analysis of the results on the detection of cases of echinococcosis, depending on the location of Veterinary control, found significant discrepancies– after post-mortem veterinary and sanitary control, only 7.26–26.27% of cases are detected, in comparison with the results of veterinary and sanitary examination of slaughter products in laboratories for veterinary and sanitary examination In the markets. This imbalance, in our opinion, can be related to homestead slaughter and poor quality post-mortem Veterinary control during it. Summarizing the obtained results of the analysis, we can conclude that the management system for the safety and quality of slaughter products in pigs in the regions of the Odessa region at the present stage still relies on the analysis of the final product, not taking into account the preventive approach.

黒毛和種肥育牛のと畜検査データを利用した腸間膜脂肪壊死症と性別，月齢及び農家の要因，その他の疾病要因との関連の解析

黒毛和種のと畜検査データ（32,586頭）を用いて，腸間膜脂肪壊死症に関連する要因を検討した．本症の発生を目的変数，月齢，性別及び農家を説明変数としたロジスティック回帰により解析したところ，本症は雌で診断されやすいこと，月齢の増加に伴い診断されやすくなること，さらに農家により大きく発症率が異なることが示された（P＜0.01）．加えて，農家を平均発症率より高いグループと低い農家グループに分け，腸間膜脂肪壊死症とその他の疾病との関連につきアソシエーション分析したところ，発症率の高い農家では，腎周囲脂肪壊死症，胃炎，出血性炎症，肺炎及び肝炎との間に統計学的に有意な相関が認められた．以上のことから，本症の発症には，性別，月齢，農家及び飼養管理方法が関連すると考えられた．

A Stochastic Model to Assess the Effect of Meat Inspection Practices on the Contamination of the Pig Carcasses.

The objective of meat inspection is to promote animal and public health by preventing, detecting, and controlling hazards originating from animals. With the improvements of sanitary level in pig herds, the hazards profile has shifted and the inspection procedures no longer target major foodborne pathogens (i.e., not risk based). Additionally, carcass manipulations performed when searching for macroscopic lesions can lead to cross-contamination. We therefore developed a stochastic model to quantitatively describe cross-contamination when consecutive carcasses are submitted to classic inspection procedures. The microbial hazard used to illustrate the model was Salmonella, the data set was obtained from Brazilian slaughterhouses, and some simplifying assumptions were made. The model predicted that due to cross-contamination during inspection, the prevalence of contaminated carcass surfaces increased from 1.2% to 95.7%, whereas the mean contamination on contaminated surfaces decreased from 1 logCFU/cm² to -0.87 logCFU/cm², and the standard deviations decreased from 0.65 to 0.19. These results are explained by the fact that, due to carcass manipulations with hands, knives, and hooks, including the cutting of contaminated lymph nodes, Salmonella is transferred to previously uncontaminated carcasses, but in small quantities. These small quantities can easily go undetected during sampling. Sensitivity analyses gave insight into the model performance and showed that the touching and cutting of lymph nodes during inspection can be an important source of carcass contamination. The model can serve as a tool to support discussions on the modernization of pig carcass inspection.

Evaluation of a synthetic peptide from the Taenia saginata 18 kDa surface/secreted oncospheral adhesion protein for serological diagnosis of bovine cysticercosis

Bovine cysticercosis is a zoonotic infection widely spread throughout Brazil, creating a burden on hygiene maintenance and the economy. Diagnosis of cysticercosis usually relies on post mortem inspection of carcasses in slaughterhouses. This detection method provides only low sensitivity. Recent advancements have improved the performance of serologic tests, such as ELISA, providing greater sensitivity and specificity. The objective of the current study was to identify and evaluate a synthetic peptide derived from the Taenia saginata 18kDa oncospheric surface protein for the diagnosis of bovine cysticercosis in ELISA. Test performance of the identified peptide was compared to an ELISA based on a heterologous crude Taenia crassiceps antigen (Tcra), widely used for the sero-diagnosis of bovine cysticercosis. Based on the primary sequence of an in silico structural model of the 18kDa protein, an epitope region designated EP1 was selected (46-WDTKDMAGYGVKKIEV-61). The peptide derived from this region yielded 91.6% (CI=80–96%) sensitivity and 90% (CI=82–95%) specificity when used in an ELISA, whereas the crude antigen yielded 70% (CI=56–8%) sensitivity and 82% (CI=73–89%) specificity. Thus, we conclude that EP1 has higher diagnostic potential for detecting bovine cysticercosis than the crude antigen Tcra.