AbstractIn the medical field, refrigeration systems are used to store and transport vaccines, blood, and other medical supplies that require specific temperature ranges to remain effective. As technology continues to advance, the demand for more efficient and sustainable refrigeration systems is also increasing. The freezer compartment is typically designed to maintain a temperature of −18°C to −23°C for storing frozen items. Consequently, this work aims to develop a hybrid refrigeration system that combines a thermoelectric cooler system (TEC) and a vapor compression refrigeration cycle (VCC) system to achieve lower temperatures than conventional refrigerators. Also, the performance of the proposed hybrid refrigeration system is experimentally assessed with various operating conditions, including varying the voltage delivered to the system. The experimental results exhibited that the temperature inside the freezer room reached −33°C, while the cold side temperature is −47°C. Also, the maximum coefficient of performance of the VCC system, TEC, and hybrid system is 2.07, 1.06, and 0.37, respectively, at a DC voltage applied of 6 V. Moreover, the results revealed that the hybrid system combining a TEC and a VCC system can be a valuable technology for specific applications with low temperatures and limited capacity requirements.