Insertion Type Research Articles

Comparative organelle genomics in Daphniphyllaceae reveal phylogenetic position and organelle structure evolution

The family Daphniphyllaceae has a single genus, and no relevant comparative phylogenetic study has been reported on it. To explore the phylogenetic relationships and organelle evolution mechanisms of Daphniphyllaceae species, we sequenced and assembled the chloroplast and mitochondrial genomes of Daphniphyllum macropodum. We also conducted comparative analyses of organelles in Daphniphyllaceae species in terms of genome structure, phylogenetic relationships, divergence times, RNA editing events, and evolutionary rates, etc. Results indicated differences in the evolutionary patterns of the plastome and mitogenome in D. macropodum. The plastome had a more conserved structure but a faster nucleotide substitution rate, and the mitogenome showed a more complex structure while the mitotic genome shows a more complex structure but a slower nucleotide substitution rate. We identified several unidirectional protein-coding gene transfer events from the plastome to the mitogenome based on homology analysis, but no transfer events occurred from the mitogenome to the plastome. Multiple TE fragments existed in organelle genomes, and two organelles showed different preferences for nuclear TE insertion types. The estimation of divergence time indicated that the differentiation of Daphniphyllaceae and Altingiaceae at around 29.86 Mya might be due to the dramatic uplift of Tibetan Plateau during the Oligocene. About 75% of codon changes in organelles were found to be hydrophilic to hydrophobic amino acids. The RNA editing in protein-coding transcripts is the result of amino acid changes to increase their hydrophobicity and conservation in alleles, which may contribute to the formation of functional 3D structures in proteins. This study would enrich genomic resources and provide valuable insights into the structural dynamics and molecular biology of Daphniphyllaceae species.

Investigating insertional code-mixing in Italian migrants in the United Kingdom: A comparative approach

Aims: This article investigates English lexical insertions in a corpus collected with first- and second-generation Italian migrants in two English cities, namely Bedford and Cambridge. It aims to establish whether, and to what extent, two Italian heritage communities share the same patterns of language alternation. Methodology/approach: I describe the frequency and type of lexical insertions to verify whether the study of lexical insertions or insertions of multiword units can shed light on the linguistic repertoire of each of the communities. The two Italian communities share many social features but differ in the ways Italians integrated or not into the host society. Data analysis: The dataset used for this contribution was collected through qualitative interviews with Italian migrants belonging to three different generations: first, second, and intermediate generations. The corpus used in this paper consists of approximately 50 hours of speech (35 hours for Bedford and 15 hours for Cambridge). I correlated the insertions of single lexemes with semantic and pragmatic specificity from a quantitative and qualitative perspective. Conclusions/results: The results provided evidence that insertion is first influenced by specificity as already Backus argued. External parameters such as migrants’ employment, ethnic composition of the social network, the size of the migrant community, and its social visibility do not, therefore, affect the borrowability of the lexemes belonging to specific domains, the frequency of which leads to their entrenchment as English lexemes even when speakers adopt the monolingual mode in Italian or dialect. The two communities do, however, differ in terms of the matrix language into which the English insertion is embedded: Italians prefer Italian as the matrix language in Cambridge but not in Bedford. Originality: The study confirms how similar Italian communities can be characterized by different linguistic usage and by a different linguistic repertoire.

Prosthesis and health-related factors of mobility in people with nontraumatic unilateral transtibial amputation.

Numerous factors can affect mobility of individuals with lower limb amputation, including prosthesis-dependent, physical and psychological determinants.Objective:This study aimed to investigate the prosthesis and health-related factors of mobility in people with nontraumatic unilateral transtibial amputation.Study Design:Cross-sectional observational study. Individuals with nontraumatic unilateral transtibial amputation who are using a prosthetic leg were included. All participants were asked to fill out the Prosthesis Evaluation Questionnaire, 12-Item Short-Form Health Survey (SF-12), Comprehensive Lower Limb Amputee Socket Survey, and 12-item Prosthetic Limb Users Survey of Mobility. The relationships between the variables of interest and mobility were investigated using correlation coefficient analyses. Multiple regression analysis was run to investigate the impact of a set of predictor variables on mobility. A total of 168 people with lower limb amputation were included. Mobility had a significant association with age, comorbidity, occupation, Prosthesis Evaluation Questionnaire score, physical and mental component scores of SF-12, subscales of the Comprehensive Lower Limb Amputee Socket Survey, educational status, amputation cause, time since amputation, time since using the prosthesis, and socket insert type. In the final model of multiple regression analysis, the Physical Component Summary score of SF-12 showed the strongest predictor of mobility (beta = 0.40, p < 0.001). Nonprosthetic factors play a significant role in mobility of people with nontraumatic unilateral transtibial amputation. Although some predictors, such as age and time since amputation, are immutable, clinicians may still enhance the mobility of this population through the utilization of health-related factors, such as improving their physical activity.

COMPREHENSIVE EVALUATION OF DIMENSIONAL DEVIATION, FLANK WEAR, SURFACE ROUGHNESS AND MATERIAL REMOVAL RATE IN DRY TURNING OF C45 STEEL

The study investigated the turning of C45 steel in a dry environment. The input parameters that were varied were cutting speed, feed, depth of cut, corner radius and insert type. The experimental investigations were carried out according to a custom experimental design using the D-optimality criterion. The measured output parameters were dimensional deviation, flank wear and surface roughness, while the material removal rate was calculated. A detailed analysis and evaluation of the effects of the input parameters on the output parameters was carried out. The model was diagnosed and appropriate regression equations was established. Based on the obtained regression equations, multi-objective optimisation was performed using particle swarm optimisation. The objective function was to simultaneously minimise dimensional deviation, flank wear and surface roughness and maximise material removal rate. The optimisation was carried out for different weighting coefficients of each output function for different production requirements. The obtained models and optimal values were verified by additional confirmation experiments.

Influence of the retropreparation ultrasonic insert type on the adhesive union of fiberglass posts within the root canal

To assess the displacement resistance of cemented fiberglass posts within the root canal, based on the type of ultrasonic retropreparation insert used during endodontic surgery. Material and methods: Thirty primary bovine incisor roots, measuring 17 mm in length, underwent endodontic treatment. After a seven-day period, the teeth were debrided and prepared to receive 13 mm posts. The roots were randomly divided into three groups, depending on the type of ultrasonic retropreparation insert used: GC – no retropreparation (control); GL – smooth insert; GD – diamond insert. The posts were cemented using RelyX U200® within the root canal. Subsequently, the roots were immersed in distilled water for 15 days and then sectioned to create specimens with an average thickness of 1.87 mm in each of the root thirds (cervical and middle). These specimens were subjected to a push-out test. Following the test, the fractured samples were examined under a stereomicroscope to determine the fracture pattern. The data obtained were analyzed using a one-way Anova test and non-parametric t-test (α = 0.05). Results: There were no statistically significant differences in the cervical third when comparing the tested ultrasonic inserts. However, in the analysis of the middle third, the use of a smooth ultrasonic insert had a significant impact on the adhesion of the intraradicular post (P &lt; 0.05). Conclusion: The application of a smooth ultrasonic insert during endodontic retropreparation significantly influenced the displacement resistance of intraradicular fiberglass posts cemented in the middle third of the canal. In the cervical third, the type of ultrasonic retropreparation insert used did not affect post retention.

Effect of gluing garment materials with adhesive inserts on their multidirectional drape and bending rigidity

Abstract The article concerns the study on multidirectional drape and bending rigidity of six wool (clothing) fabrics with two types of adhesive inserts. The aim of research was to investigate the effect of joining woolen fabrics with “Freudenberg Vilene” adhesive inserts on the bending rigidity and multidirectional drape. The adhesive inserts were differentiated according to mass per square meter, while the fabrics were differentiated according to thickness, weave, and mass per square meter. Based on the scientific literature, the issues related to the methods of testing the bending rigidity and multidirectional drape coefficient were discussed. All the conducted tests were aimed at the proper selection of adhesive inserts for the designed clothing products. The use of adhesive inserts affects the bending stiffness and multidirectional drapeability of clothing material. The fabric weave significantly affects the values of bending rigidity and drapeability. As the thickness of adhesive inserts increases, the overall bending rigidity increases. The greater the fabric mass per square meter, the stiffer the fabric. As the mass per square meter and thickness increase, the multidirectional rigidity of fabrics increases. The value of bending rigidity for the plain fabric is higher compared to twill fabric.

CRISPR/Cas9-Mediated BocPDSs Gene Editing in Chinese Kale Using the Endogenous tRNA-Processing System

Chinese kale is a native vegetable from the Brassicaceae family that is grown extensively in Southeast Asia and Southern China. Its low genetic transformation and gene editing efficiency hinder gene function research and molecular biology in Chinese kale. CRISPR/Cas9 is a useful tool for plant genome research due to its rapid development and optimization. This study targeted BocPDSs, (BocPDS1, BocPDS2) to establish an effective CRISPR/Cas9 system in Chinese kale. A tandemly arranged tRNA-sgRNA construct was used to express numerous sgRNAs to induce BocPDS1 and BocPDS2 double and single mutations, with a mutation rate of 61.11%. As predicted, several mutant plants showed an albino phenotype with a harbored mutation in an exon and intron region, highlighting the relevance of the intron. The presence of mutations in the intron region suggests that the cleavage process in Chinese kale, utilizing CRISPR/Cas9 shows a preference for AT-rich regions. The distinct and somewhat redundant functions of BocPDS1 and BocPDS2 are demonstrated by the complete albino phenotype of the double mutants and the mosaic albino phenotype of the individual BocPDS1 and BocPDS2 mutants. Specific gene editing modes, including base deletion, base substitution, and base insertion, were identified in the sequence of the target gene. Among them, short nucleotide insertions were the most common type of insertion, with base insertions having the highest frequency (61.54%). Furthermore, no instances of off-target gene editing were detected. The current work demonstrated that the CRISPR/Cas9 gene editing system, which relies on endogenous tRNA processing, can effectively induce mutagenesis in Chinese kale. This finding establishes a theoretical basis and technical backbone for the more effective implementation of CRISPR/Cas9 gene-editing technology in Chinese kale and Brassica plants.

Structure-based clustering and mutagenesis of bacterial tannases reveals the importance and diversity of active site-capping domains.

Tannins are critical plant defense metabolites, enriched in bark and leaves, that protect against microorganisms and insects by binding to and precipitating proteins. Hydrolyzable tannins contain ester bonds which can be cleaved by tannases-serine hydrolases containing so-called "cap" domains covering their active sites. However, comprehensive insights into the biochemical properties and structural diversity of tannases are limited, especially regarding their cap domains. We here present a code pipeline for structure prediction-based hierarchical clustering to categorize the whole family of bacterial tannases, and have used it to discover new types of cap domains and other structural insertions among these enzymes. Subsequently, we used two recently identified tannases from the gut/soil bacterium Clostridium butyricum as model systems to explore the biochemical and structural properties of the cap domains of tannases. We demonstrate using molecular dynamics and mutagenesis that the cap domain covering the active site plays a major role in enzyme substrate preference, inhibition, and activity-despite not directly interacting with smaller substrates. The present work provides deeper knowledge into the mechanism, structural dynamics, and diversity of tannases. The structure-based clustering approach presents a new way of classifying any other enzyme family, and will be of relevance for enzyme types where activity is influenced by variable loop or insert regions appended to a core protein fold.

Modelling and optimization of dimensional accuracy and surface roughness in dry turning of Inconel 625 alloy

The CNC dry turning of Inconel 625 alloy was investigated in this study. Turning was performed using different feeds, corner radii, and insert types. The dimensions and the arithmetic mean surface roughness were measured before and after turning. The influence of the input parameters and the process modelling were evaluated using a full factorial design of experiments. Prediction models were developed, and process optimization was carried out to simultaneously maximize dimensional accuracy and surface quality. The optimal values of the input parameters were identified for the wiper insert, a feed rate of 0.1 mm/rev, and a corner radius of 0.8 mm. Under these conditions, the optimal deviation from the specified dimension was 0.2 mm, while the surface roughness was 0.297 µm. During the confirmation phase, the mean percentage errors were 0.9 % for surface roughness and 3.45 % for dimensional deviation. The percentage errors observed in the confirmation experiments, all of which were below 5 %, demonstrate the feasibility of using the proposed approach for modelling and optimizing the turning of Inconel 625 alloy.

Prevalence and Pattern of Labial Frenal Attachments in Adult Nigerians - a Pilot Study

Introduction The frenum is a band of mucous membrane which connects the lips and cheeks to the alveolar mucosa, underlying periosteum, or the gingiva with a primary function of stabilizing the lips and tongue. There are variations of frenal attachment and morphologies, some associated with health, others resulting to loss of periodontium. This study therefore aims to assess the various attachments and morphologies within our study population. Materials and Methods This was a descriptive cross-sectional study conducted at the Oral Diagnosis and Periodontology clinics of the Lagos University Teaching Hospital Idi-Araba among participants who reported to both clinics. Convenience sampling method was employed in participants’ selection, and interviewer-administered questionnaires were used for data collection and documentation of socio-demographic variables, and findings of intra-oral examination. Ethical approval was obtained from the institutional Health Research Ethics Committee and informed consent was obtained from each participant. Data was analyzed using the Statistical Product and Service Solutions (SPSS) version 27. P values &lt; 0.05 were statistically significant. Results The study comprised 337 participants, 203 (60.2%) were females. Mean age was 34.6±15.2 years. The frenal attachment distribution in the maxilla was mucosal 204 (60.5%), gingival 105 (31.2%), papillary 23 (6.8%), and papillary penetrating 5 (1.5%);while in the mandible, 233 (69.1%), 92 (27.3%), 9 (2.7%) and 3 (0.9%) presented with mucosal, gingival, papillary, and papillary penetrating frenal attachment respectively. Simple frenum was the most prevalent frenum morphology in the maxilla [328 (97.3%)] and the mandible [321 (95.2%)], followed by trifid frenum in both maxilla [5 (1.5%)] and mandible [4 (1.2%)]. Diastema was present in 27.6% in maxilla and 11.0% in the mandible. Conclusion In this study, mucosal type was predominant among all age groups. Males presented more with gingival and mucosal type of frenal insertion, while females presented more with papillary type of frenal insertion. Papillary and/or papillary penetrating were significantly associated with the presence of diastema.

Heat transfer and pressure drop comparison for corrugated tube and different numbers of copper foam cylindrical inserts in laminar flow

AbstractThe chief objective of the present investigation is to study experimentally the hydraulic and thermal performance of a corrugated tube with copper foam cylindrical inserts (MFCIs) containing a new type of inserts with the purpose to improve the procedure of heat transfer, which gives an elevated thermal enhancement factor more than that of smooth tube beneath the similar operating circumstances. Also, in this paper, the study of the effect on the number of cylindrical inserts was made from metal foam (10 PPI and porosity 0.9) on the Nusselt number and Nusselt number ratio as a double heat exchanger furnished with the suggested MFCI with various values of Reynolds numbers ranges from 1600 to 4000 via using water as the test fluid. The investigational outcomes revealed an enhancement in heat transfer as well as the thermal performance of the corrugated tube with MFCI, which being significantly augmented in comparison to those for the smooth tube. Additionally, the average rise in the value of heat transfer is within 225% and 400% at the test range, relying upon the number of cylindrical inserts as well as the Reynolds number, whereas the maximum thermal performance is obtained to be around 2.4 for utilizing the corrugated tube having (four) cylindrical inserts at low Reynolds number (1820). Furthermore, the outcome of the loss of pressure manifested that the corrugated tube's mean friction factor is between 96% and 97% more than the smooth tube.

Comparing results of midline catheter and peripherally inserted central catheter in cancer patients under chemotherapy.

Peripherally inserted central catheters (PICC), and peripherally inserted midlines (MC) represent a possible less invasive alternative option to totally implantable vascular access devices (TIVAD) in cancer patients. This study aims to investigate the outcomes of PICC, and MC devices in patients undergoing chemotherapy. A prospective non-concurrent observational study was conducted at the IRCCS Ospedale Policlinico San Martino, Genova (Italy) on 559 cancer patients candidate to chemotherapy bearing PICC, and MC during the period 2019-2022. The primary endpoint was the comparative analysis for failure of the different types of peripheral insertion accesses requiring removal. Secondary outcome measures were age at diagnosis, gender, period of diagnosis, cancer site and type of chemotherapy. The negative binomial regression analysis was applied to estimate catheter removal rate ratios (RR), along with 95% confidence limits (95% CL), in each category of the risk factors. The median follow-up time was 2.6 months (IQR = 1.4-4.6; min-max = 0.03-12.7), and at the end of the study period, a total of 45 catheter failures were detected. A risk reduction of approximately 75% (RR = 0.24; 95% CL = 0.11-0.51) was emphasized for patients with PICC when compared with those with MLC. Type of chemotherapy and cancer site appeared to be important predictive factors for catheter removal risk. For cancer patients undergoing chemotherapy by a non TIVAD, PICC must be preferred to MC because of higher safety and efficacy.

Maximizing efficiency in C45 steel machining: an integrated AI-based approach to coated insert optimization

AbstractMachining involves the subtraction of the material from the sample workpiece to achieve the desired shape or surface. This versatile method is capable of producing a wide range of parts, varying from simple to intricate profiles. Coating materials are increasingly being utilized in tool inserts in the production industry owing to their superior thermal properties and wear resistance. The shielding of hard coatings, with thicknesses of only a few microns, enhances performance and durability. In this study, machining of C45 steel using distinct coated inserts was explored. The experimental trials employed PVD and CVD methods for coated tungsten carbide (WC) tools/inserts and PVD-coated cermet tools/inserts with different machining parameters. Performance metrics, such as the surface finish and reliability of the tool, were considered for the evaluation. The average tool life variation between the PVD-coated cermet and PVD-coated WC was 178.86%, and 30.11% between the PVD-coated cermet and CVD-coated WC at 1 mm DOC. ANOVA was performed using Response Surface Methodology to explore the influence of input parameters on output. The results indicate that the depth of machining and spindle speed significantly influence Ra, whereas spindle speed and type of tool insert have a considerable impact on the life span of the tool. The developed mathematical model for the prediction of tool life and Ra indicates its potential for performance forecasting during C45 steel machining. Grey relation analysis was employed to optimize the process parameters. Optimal results were achieved with a spindle speed of 400 m/min, 0.5 mm depth of cut, and cermet tool inserts. PVD-coated WC inserts performed better. ANFIS was applied for the prediction and optimization of the machining parameters.

Morphometric Analysis of Extensor Hallucis Longus Muscle - A Cadaveric Study

Abstract Background: Extensor hallucis longus (EHL) is the muscle of the anterior compartment of the leg. It has origin from the medial surface of the fibula; insertion is on the base of the distal phalanx of the big toe. Action of the muscle is an extension of the phalanges of the big toe and dorsiflexion of the foot. Tendon grafting is a very common procedure done in branches such as plastic surgery, maxillofacial surgery, and lacerated wounds of tendons where extra tendons will preserve the function of muscle. EHL is one of the preferred tendons for this purpose. The purpose of this study was to observe variations in the morphology of EHL. Methodology: The study was performed on a total 85 numbers of lower limbs available in the department of anatomy. The sample contains 46 female and 34 male cadavers. Attachments of the EHL were studied. Results: Three different types of insertion of EHL were observed: Type 1 - The most common type, insertion on the extensor hood on the dorsum of the distal phalanx, Type 2 - Two distal tendons while insertion, and Type 3 - Trilaminar insertion. Conclusion: The EHL is vital for tendon grafting in reconstruction surgeries. Its close anatomical relationship with the tibialis anterior and extensor digitorum longus highlights the need for precise morphological knowledge. Accessory tendons can be utilized without significant clinical deficits. Preoperative radiological assessment is crucial to confirm donor muscle morphology.

Variants in the superior attachment of uncinated process and their corresponding frontal sinus drainage pattern: a computed tomography-based retrospective clinical study

Background: Understanding the morphology and spatial relationships of the uncinate process and its associated spaces is crucial for diagnosing and treating conditions affecting sinus drainage and ventilation. This consistent anatomical structure is an important surgical landmark for endoscopic sinus surgery. Methods: Computed tomography (CT) records of 100 patients over two years were extracted via the hospital's PACS system. The type of superior insertion of uncinate process (UP) was observed in coronal cuts of CT scan images, and classification was done based on the Friedmann and Landsberg classification system and tabulated. The corresponding variations in the frontal sinus outflow tract were noticed, and their effect on frontal sinusitis was recorded. Sinusitis of the frontal sinus was in guidance with EPOS 2020 (mucosal changes within the ostiomeatal complex or sinus). Results: Type 2 was the most common of uncinate attachments (39.5%). The least common variant was type 5 (4%). Medial-type frontal sinus drainage pathway was observed in 78.5% of patients. Sinusitis was present in 23.5% of our study population. Type 2 had a 25% contribution to the occurrence of sinusitis. The medial frontal sinus drainage pathway contributes 24% to sinusitis with a significant p value of 0.010. Conclusions: Uncinate process, with its varying superior attachment, influences the frontal sinus outflow tract and may contribute to the development of frontal sinusitis. Awareness of the possible variations in the attachment of the uncinate process is vital in the preoperative planning process, which will serve as a road map to surgeons and aid in preparedness for intraoperative complications.

Improving the Performance of the Machining Process by Using Ultra‐Advanced Tools in a Clean Turning of Inconel 686 Using the Minimum Quantity Lubrication Method

ABSTRACTThe high tensile strength and high resistance of nickel‐based superalloy 686 against high temperatures and corrosion rates have made it a widely used in important applications such as the aerospace industry, high pollution‐ and corrosion‐resistance equipment manufacturing and petrochemical industry. Therefore, the machining of this advanced alloy with its unique properties is extremely important and can be challenging. Significant increase in input parameters levels, reduction of machining costs, improvement of surface and subsurface properties and clean production are among the issues that should be considered in dealing with Inconel 686 turning operations. Simultaneous application of advanced tools such as polycrystalline diamond (PCD) and polycrystalline cubic boron nitride (PCBN) and optimised minimum quantity lubrication (MQL) method and evaluating the results obtained with a wide range of output parameters related to machining process performance and tribological properties can be proposed as an innovation and a solution to this problem in this article. This study analyses several output parameters with different speeds and feeds to evaluate the effect of cutting insert type on machining process performance and tribological properties. The output parameters include tool wear, residual stress, cutting zone temperature, surface smoothness, machining forces and workpiece surface defects. The results indicated that using the optimised MQL method reduces the size of lubricant droplets and increases the surface covered by cooling. With these changes, the performance of the machining process and the parameters related to the surface integrity increase significantly. Among the parameters associated with the performance of the machining process, the PCD tool reduces the cutting zone temperature by 23%, the tool wear by 19% and the machining forces by 18% compared to the PCBN tool. In the parameters related to surface integrity, this method reduces the residual stress by 19% and the surface roughness by 9% compared to the PCBN tool. From the production index perspective, the PCD tool can significantly increase the cutting speed and feed rate, reducing production time and costs.

Self-perception of oral health, gender and employment among Brazilian population.

Self-perception of oral health is a recognized indicator, based on a single question. Association among oral health and socioeconomic status has been well established, however in Brazil, no results were found on the relation with type of insertion in labor market. The aim of this study was to describe the prevalence of negative self-perception of oral health and associated factors, according to gender among Brazilian workers population, in 2019. The National Health Survey, nationwide household-based survey, carried out in 2019, was used as source of data. Absolute and relative frequencies were calculated to understand the distribution of variables of interest and characterize the sample (n = 54,343). Binary logistic regression was used in the analyses. Odds ratio (OR) was estimated using a 95% confidence interval in bivariate and multivariate analyses. The prevalence of negative self-perception of oral health was 29.3%; in that, 31.0% among men and 27.2% among women. Compared to formal employees, the chance of negative self-perception of oral health among the informal workers was almost 20% higher among men and 13% among women after adjusting for all confounding factors. Unemployed women were 33% more likely to have negative self-perception of oral health. Self-reported unemployment increased the chance of a negative self-perception of oral health among women; however, the same effect was not observed among men. Why is there a difference? The association between informal work and negative SEOH is relevant in the Brazilian context of work deregulation and growth in the number of people without access to formal employment. The results suggested addressing employment conditions in adult oral health promotion actions, with emphasis on gender differences.

Intra-Host Citrus Tristeza Virus Populations during Prolonged Infection Initiated by a Well-Defined Sequence Variant in Nicotiana benthamiana.

Due to the error-prone nature of viral RNA-dependent RNA polymerases, the replication of RNA viruses results in a diversity of viral genomes harboring point mutations, deletions, insertions, and genome rearrangements. Citrus tristeza virus (CTV), a causal agent of diseases of economically important citrus species, shows intrinsic genetic stability. While the virus appears to have some mechanism that limits the accumulation of single-nucleotide variants, the production of defective viral genomes (DVGs) during virus infection has been reported for certain variants of CTV. The intra-host diversity generated during plant infection with variant T36 (CTV-T36) remains unclear. To address this, we analyzed the RNA species accumulated in the initially infected and systemic leaves of Nicotiana benthamiana plants inoculated with an infectious cDNA clone of CTV-T36, which warranted that infection was initiated by a known, well-defined sequence variant of the virus. CTV-T36 limited the accumulation of single-nucleotide mutants during infection. With that, four types of DVGs-deletions, insertions, and copy- and snap-backs-were found in all the samples, with deletions and insertions being the most common types. Hot-spots across the genome for DVG recombination and short direct sequence repeats suggest that sequence complementarity could mediate DVG formation. In conclusion, our study illustrates the formation of diverse DVGs during CTV-T36 infection. To the best of our knowledge, this is the first study that has analyzed the genetic variability and recombination of a well-defined sequence variant of CTV in an herbaceous host.

A Review of Central Vein Catheter for Hemodialysis: Insertion Sites and Techniques

Chronic kidney disease (CKD) is a global epidemic, being increasingly prevalent in both developed and developing countries, including Indonesia. Hemodialysis (HD) is the primary renal replacement therapy, along with peritoneal dialysis and kidney transplantation. The effectiveness of HD relies on well-functioning vascular access, such as central vein catheters (CVCs). These catheters are classified by their duration of use: short-term, medium-term, and long-term. They are also distinguished by insertion type (central or peripheral), insertion site (jugular, subclavian, femoral), and number of lumens (single, double, triple). The preferred site for insertion is the internal jugular vein, followed by the femoral vein and the subclavian vein. Techniques for CVC insertion include the central approach, the posterior approach, and others. This article reviews the role of CVC for vascular access in HD. Specifically, various CVC insertion sites and techniques will be examined. The authors will also discuss the available research on the application of CVC as vascular access for HD in Indonesia.

Multivendor comparison of quantification accuracy of effective atomic number by Dual-Energy CT: A phantom study

PurposeOur study aimed to compare the accuracy of the effective atomic number (Zeff) of five dual-energy CT (DECT) from three vendors and different generations under different scanning parameters. MethodsZeff accuracy of five DECT scanners with twelve tube voltage configurations was evaluated by using the TomoTherapy cheese phantom. The potential dose dependence of the Zeff was investigated using three radiation dose (5, 15, and 25 mGy), and the robustness of Zeff was simulated for different organs of the body by placing the inserts at different positional depths. Bias and mean absolute percentage error (MAPE) were used to characterize the accuracy of Zeff. Data underwent analysis using one-way ANOVA, followed by the Turky and LSD post hoc tests, simple linear regression, and linear mixed models. ResultsAll tube voltage configurations had a bias of less than 1. Dual layer detector DECT (dl-DECT) −140 kV has the lowest MAPE (1.79 %±1.93 %). The third generation dual source DECT (ds-DECT) and the second generation rapid switch DECT (rs-DECT) have higher MAPE than their predecessor DECT. The results of the linear mixed model showed that tube voltage configuration (F=16.92, p < 0.001) and insert type (F=53.26, p < 0.001) significantly affect the MAPE. In contrast, radiation dose only has a significant effect on the MAPE of rs-DECT. The inserts position does not affect the final MAPE. ConclusionWhen scanning different inserts, Zeff accuracy varies by vendor and DECT generation. Of all the scanners, dl-DECT had the highest Zeff accuracy. Upgrading DECT generation doesn’t lead to higher accuracy, or even lower.