Mode Of Insertion Research Articles

Designing V Single Atom Embedded Carbon Moiety for the Electrocatalytic Nitrogen Reduction Reaction by First Principles Study.

Development of an efficient electrocatalyst for the nitrogen reduction reaction (NRR) to serve as a sustainable alternative to the Haber-Bosch process has proven highly challenging. Single atom catalysts (SACs), which have the maximum atom utilization efficiency, are among the most promising candidates. Single atoms can be incorporated to a catalytic system by doping or substitution or by attaching a molecular coordination complex to a substrate and the different insertion modes allow the chemical environment to be varied. We have used DFT to investigate vanadium SACS for NRR activity with a focus on varying the coordination environment of the V atom. Phthalocyanine, porphyrin and graphene like coordination environments with varying N-coordination have been studied. Vanadium phthalocyanine (VphN4) is the most promising of the investigated systems. It features high selectivity relative the HER reaction and relatively strong binding of N2 relatively H, which prevents poisoning of the surface by hydrogen. VphN4 also has the lowest overpotential among the studied systems. The electrocatalytic properties of VphN4 deposited as a monolayer on the Ag (111) surface have been investigated. This system, which already has been prepared, shows promising properties for use as a catalytic electrode for the NRR reaction.

Mechanism and Selectivities of [Cu]/[M] (M=Pd, Ni) Synergistic Catalyzed Alkene Arylboration: A DFT Investigation

ABSTRACTThe transition metal–catalyzed boronation reactions are considered as one of the most effective methods for the synthesis of alkylboranes in terms of functional group compatibility and reactivity. Herein, the mechanism of synergistic catalysis of alkene arylboration by [Cu]/[M] (M=Pd, Ni) bimetallic catalysts, as well as the regioselectivity and stereoselectivity of the reactions, has been investigated by using DFT calculations. The results show that four processes are involved in the whole reaction: [Cu]‐catalyzed borylcuprization of alkene, [Pd] or [Ni]‐catalyzed oxidative addition of halogenated aryl groups, transmetalation, and reductive elimination. [Cu]/[Ni] bimetallic catalysts show the same catalytic activity as [Cu]/[Pd] catalysts and are good economic alternatives for the alkene arylboration. The cleavage of B–B bond of B2Pin2 is the rate‐determining step. The regioselectivity of the title reaction is determined by the mode of the alkene insertion into the Cu–B bond. The attacking of C atom with the more electron‐rich character in alkyne makes for the formation of the main product. The small energy barrier difference between the rotation of the Cu‐C σ bond from the syn‐9 to the anti‐9 controls the stereoselectivity. Our findings provide a thorough explanation of the experimental results and shed light on the further development of the alkene functionalization.

Evaluation of the immune responses of biological adjuvant bivalent vaccine with three different insertion modes for ND and IBD

ABSTRACT Infectious bursal disease (IBD) is a widespread problem in the poultry industry, and vaccination is the primary preventive method. However, moderately virulent vaccines may damage the bursa, necessitating the development of a safe and effective vaccine. The Newcastle disease virus (NDV) has been explored as a vector for vaccine development. In this study, reverse genetic technology was used to obtain three recombinant viruses, namely, rClone30-VP2L (P/M)-chGM-CSF (NP), rClone30-chGM-CSF (P/M)-VP2L (NP), and rClone30-VP2L-chGM-CSF (P/M). Animal experiments showed that the three biological adjuvant bivalent vaccines effectively increased anti-NDV and anti-infectious bursal disease virus (IBDV) titres, enhancing both humoral and cellular immune responses in chickens without leading to any harm. Amongst the three biological adjuvant bivalent vaccines, the rClone30-chGM-CSF (P/M)-VP2L (NP) group had higher levels of anti-NDV antibodies at 14 days after the first immunization and stimulated a greater humoral immune response in 7–10 days. While, the rClone30-VP2L (P/M)-chGM-CSF (NP) group was the most effective in producing a higher level of IBDV antibody response. In conclusion, these three vaccines can induce immune responses more rapidly and effectively, streamline production processes, be cost-effective, and provide a new avenue for the development of Newcastle disease (ND) and IBD bivalent vaccines

Morphological study of the popliteus muscle-tendon complex in formalin embalmed adult cadavers.

The dimensions of popliteus muscle and its tendon are subjected to variability in the origin, mode of insertion, innervation patterns and vascular supply. The aim of this study was to measure the length, thickness and width of the popliteus muscle and its tendon at its different parts. The objectives were to study the topographic anatomy of the neurovascular structures of the popliteus and also to determine the dimensions of the popliteo-fibular ligament. This descriptive cross sectional institutional based study included 50 formalin embalmed adult lower limb specimens. The measurements were performed by using the digital Vernier caliper. The length of the popliteus muscle belly along the upper and lower border were 44.2±6.63 mm and 89.26±14.41 mm, width of the muscle belly at midpoint, musculotendinous junction and insertion were 28.45±6.85 mm, 11.7±3.5 mm and 75.95±10.7 mm. The thickness of muscle belly at the midpoint was 2.55±0.55 mm. The length of popliteal tendon, width at origin and at musculotendinous junction were 24.85±2.15 mm, 7.55±1.55 mm and 8.5±1.15 mm. The thickness of tendon of popliteus was 2.6±0.75 mm. The length of nerve to popliteus was 50.44±8.66 mm and its origin was located 27.54±6.18 mm from the intercondylar line. The distance of origin of medial and lateral geniculate arteries from the intercondylar line were 26.26±10.47 mm and 20.76±5.19 mm. The distance of division of popliteal artery was 49.44±16.26 mm from the intercondylar line. The length and width of the popliteo-fibular ligament was 17.84±3.43 mm and 7.36±1.9 mm individually. This study offered detailed morphometric data of the popliteus and it is believed that the data of this anatomical research is enlightening to orthopedic surgeons particularly in the field of arthroscopic and plastic surgery. The data can be considered as the database from our population.

Free-standing microscale photonic lantern spatial mode (De-)multiplexer fabricated using 3D nanoprinting

Photonic lantern (PL) spatial multiplexers show great promise for a range of applications, such as future high-capacity mode division multiplexing (MDM) optical communication networks and free-space optical communication. They enable efficient conversion between multiple single-mode (SM) sources and a multimode (MM) waveguide of the same dimension. PL multiplexers operate by facilitating adiabatic transitions between the SM arrayed space and the single MM space. However, current fabrication methods are forcing the size of these devices to multi-millimeters, making integration with micro-scale photonic systems quite challenging. The advent of 3D micro and nano printing techniques enables the fabrication of freestanding photonic structures with a high refractive index contrast (photopolymer-air). In this work we present the design, fabrication, and characterization of a 6-mode mixing, 375 µm long PL that enables the conversion between six single-mode inputs and a single six-mode waveguide. The PL was designed using a genetic algorithm based inverse design approach and fabricated directly on a 7-core fiber using a commercial two-photon polymerization-based 3D printer and a photopolymer. Although the waveguides exhibit high index contrast, low insertion loss (−2.6 dB), polarization dependent (−0.2 dB) and mode dependent loss (−4.4 dB) were measured.

Compact adiabatic mode (de)multiplexer using a subwavelength grating waveguide in the silicon-on-insulator platform

Mode-division multiplexing technology holds great promise for addressing the ever-increasing demand for data transmission capacity. This paper proposes a compact adiabatic mode (de)multiplexer [(de)MUX] using a subwavelength grating (SWG) waveguide in the silicon-on-insulator platform, with optimization for the TE0-TE1 and TE0-TE2 mode (de)MUXs. By introducing an SWG waveguide, the coupling strength of waveguides can be enhanced, making it possible for the realization of more compact adiabatic mode (de)MUXs. The adiabatic evolution lengths for TE0-TE1 and TE0-TE2 mode (de)MUXs are only 10 µm and 17.5 µm, respectively. The numerical simulation shows that the operating bandwidth of the proposed device is beyond 200 nm. For the TE0-TE1 mode (de)MUX, the insertion loss and mode crosstalk of TE0-TE1 mode conversion within the whole wavelength range of 1450–1650 nm are less than 0.25 dB and −32.0dB, respectively. For the TE0-TE2 mode (de)MUX, the insertion loss and mode crosstalk of TE0-TE2 mode conversion within the entire bandwidth are less than 0.26 dB and −27.4dB, respectively. The TE0-TE1 and TE0-TE2 mode (de)MUXs can be cascaded to form a three-mode (de)MUX. For TE0-TE0, TE0-TE1, and TE0-TE2 processes within the entire bandwidth, the mode crosstalks are less than −33.5dB, −26.1dB, and −25.8dB, respectively. The device is scalable to the conversion of TE0 mode to higher-order mode.

Morphological study of the popliteus muscle-tendon complex in formalin embalmed adult cadavers

Background The dimensions of popliteus muscle and its tendon are subjected to variability in the origin, mode of insertion, innervation patterns and vascular supply. The aim of this study was to measure the length, thickness and width of the popliteus muscle and its tendon at its different parts. The objectives were to study the topographic anatomy of the neurovascular structures of the popliteus and also to determine the dimensions of the popliteo-fibular ligament. Methods This descriptive cross sectional institutional based study included 50 formalin embalmed adult lower limb specimens. The measurements were performed by using the digital Vernier caliper. Results The length of the popliteus muscle belly along the upper and lower border were 44.2±6.63 mm and 89.26±14.41 mm, width of the muscle belly at midpoint, musculotendinous junction and insertion were 28.45±6.85 mm, 11.7±3.5 mm and 75.95±10.7 mm. The thickness of muscle belly at the midpoint was 2.55±0.55 mm. The length of popliteal tendon, width at origin and at musculotendinous junction were 24.85±2.15 mm, 7.55±1.55 mm and 8.5±1.15 mm. The thickness of tendon of popliteus was 2.6±0.75 mm. The length of nerve to popliteus was 50.44±8.66 mm and its origin was located 27.54±6.18 mm from the intercondylar line. The distance of origin of medial and lateral geniculate arteries from the intercondylar line were 26.26±10.47 mm and 20.76±5.19 mm. The distance of division of popliteal artery was 49.44±16.26 mm from the intercondylar line. The length and width of the popliteo-fibular ligament was 17.84±3.43 mm and 7.36±1.9 mm individually. Conclusions This study offered detailed morphometric data of the popliteus and it is believed that the data of this anatomical research is enlightening to orthopedic surgeons particularly in the field of arthroscopic and plastic surgery. The data can be considered as the database from our population.

Reappraisal of the anatomical diversities of the pyramidalis muscle with their potential clinical applicability: cadaveric analysis.

The present study examined the morphology and morphometric parameters of the pyramidalis muscle (PM) in detail with their potential applicability in making midline infra-umbilical incisions and biomechanics of the linea alba. PM was examined in 51 formalin-fixed cadavers (36 males and 15 females), and based on the mode of insertion or the level of apex, the formation or shape of the muscle was classified into nine types (Mori's classification). Bilateral PM was more prevalent (39.21%) than unilateral (1.96%) (p = 0.001). All the cases showed side symmetry except one. Mori's type 7 (right PM is higher apex and the left PM with elongated origin) was the most common form. The mean length of PM in males and females was 4.51 ± 0.14 and 3.33 ± 0.12cm on the right and 4.51 ± 0.11 and 3.26 ± 0.16cm on the left side. The mean width of right-sided PMs in males and females was 1.90 ± 0.17 and 1.58 ± 0.13cm and left-sided 1.88 ± 0.14 and 1.55 ± 0.38cm. The mean of pyramidalis-pubo-umbilical index (PPI) in males and females was 32.82 ± 1.65 and 27.50 ± 1.08, respectively. The mean insertion angle was 24.56 ± 3.07 on right side and 23 ± 2.03 on the left side (p = 0.03). Male predominance existed on right- and left-sided PM length (p < 0.001 and p < 0.001), width (p = 0.001) and PPI (p = 0.001). The strong positive correlation (r = 0.83) between length and width indicates a symmetrical muscle augmentation in the two dimensions. PM is an inconsistent anatomical structure with persistent morphology. The level and angle of insertion into the linea are crucial in the biomechanics of linea alba. PPI, determining the termination level would be useful to surgeons making midline infra-umbilical incisions.

YidC from Escherichia coli Forms an Ion-Conducting Pore upon Activation by Ribosomes.

The universally conserved protein YidC aids in the insertion and folding of transmembrane polypeptides. Supposedly, a charged arginine faces its hydrophobic lipid core, facilitating polypeptide sliding along YidC's surface. How the membrane barrier to other molecules may be maintained is unclear. Here, we show that the purified and reconstituted E. coli YidC forms an ion-conducting transmembrane pore upon ribosome or ribosome-nascent chain complex (RNC) binding. In contrast to monomeric YidC structures, an AlphaFold parallel YidC dimer model harbors a pore. Experimental evidence for a dimeric assembly comes from our BN-PAGE analysis of native vesicles, fluorescence correlation spectroscopy studies, single-molecule fluorescence photobleaching observations, and crosslinking experiments. In the dimeric model, the conserved arginine and other residues interacting with nascent chains point into the putative pore. This result suggests the possibility of a YidC-assisted insertion mode alternative to the insertase mechanism.

The Organic Crisis of a Colonised State: Bulgaria in the Eye of the Pandemic, 2020–2022

Abstract Bulgaria’s particularly tragic experience with covid-19, with one of the largest death tolls per 100,000 in Europe, can be analysed in terms of purely natural factors (more lethal variants), the foibles and missteps of leaders, and unpredictable vaccine supplies. From a Gramscian critical materialist perspective, the pattern is better understood in terms of four interlocking structures: Bulgaria’s subaltern status as a downtrodden colony of Europe, its mode of insertion into globalised capitalism, the Orthodox Church’s strenuous effort to maintain its hegemonic position within Bulgarian nationalism, and the crisis of hegemony that engulfed a government already reliant on corruption more than persuasion, with an inchoate and ideologically variegated ‘Great National Revolt’ against it coinciding with the pandemic. The upshot of these structures was a rate of vaccination that was unusually low by European standards, leaving many Bulgarians, especially the elderly, lethally exposed to the disease and harmed by a widely distrusted corrupt neoliberal state.

Evaluation of an early-stage prototype polyurethane femoral head implant for hip arthroplasty

IntroductionThi study evalautes a new bone-preserving femoral head cover that mimics the articular cartilage of the femoral head. MethodsA specially developed polyurethane (PU) was evaluated in biocompatibility (cytotoxicity test) and mechanical response to tensile loading. In the cytotoxicity test, steam sterilized (SS) and ethylene oxide sterilized (EtO) PU samples were incubated separately in a cell culture medium. The seeded cell line MG-63 was then added to these sample-incubated cell culture mediums. One negative control group and one positive control group were also evaluated. The cells in each group were cultured for seven days before being quantified using the alamarBlue assay. In the mechanical test, the femoral head cover implants were separated into three groups of three samples. Each group represented a different implant insertion idea: direct insertion (uc sample) and another two insertion modes (is and ss samples) representing implants with enclosure mechanisms. The test consisted of distance-controlled cyclic tensile loadings followed by a failure test. ResultsThe cytotoxicity test results show no significant difference in fluorescence intensity between the negative control, the three SS groups, and one EtO group (P > 0.05). However, the other two EtO groups exhibit significantly lower fluorescence intensity compared with the negative control (P < 0.05). In the mechanical test, the is samples have the highest cyclic loading force at 559.50 ± 51.41 N, while the uc samples exhibit the highest force in the failure test at 632.16 ± 50.55 N. There are no significant differences (P > 0.05) among the uc, is, and ss groups in terms of stiffness. ConclusionThe cytotoxicity test and the mechanical experiment provide initial assessments of the proposed PU femoral head cover implant. The evaluation outcomes of this study could serve as a foundation for developing more functional design and testing methods, utilizing numerical simulations, and developing animal/clinical trials in the future.

Morphological study of the popliteus muscle-tendon complex in formalin embalmed adult cadavers

Background: The dimensions of popliteus muscle and its tendon are subjected to variability in the origin, mode of insertion, innervation patterns and vascular supply. The aim of this study was to measure the length, thickness and width of the popliteus muscle and its tendon at its different parts. The objectives were to study the topographic anatomy of the neurovascular structures of the popliteus and also to determine the dimensions of the popliteo-fibular ligament. Methods: This descriptive cross sectional institutional based study included 50 formalin embalmed adult lower limb specimens. The measurements were performed by using the digital Vernier caliper. Results: The length of the popliteus muscle belly along the upper and lower border were 44.2±6.63 mm and 89.26±14.41 mm, width of the muscle belly at midpoint, musculotendinous junction and insertion were 28.45±6.85 mm, 11.7±3.5 mm and 75.95±10.7 mm. The thickness of muscle belly at the midpoint was 2.55±0.55 mm. The length of popliteal tendon, width at origin and at musculotendinous junction were 24.85±2.15 mm, 7.55±1.55 mm and 8.5±1.15 mm. The thickness of tendon of popliteus was 2.6±0.75 mm. The length of nerve to popliteus was 50.44±8.66 mm and its origin was located 27.54±6.18 mm from the intercondylar line. The distance of origin of medial and lateral geniculate arteries from the intercondylar line were 26.26±10.47 mm and 20.76±5.19 mm. The distance of division of popliteal artery was 49.44±16.26 mm from the intercondylar line. The length and width of the popliteo-fibular ligament was 17.84±3.43 mm and 7.36±1.9 mm individually. Conclusions: This study offered detailed morphometric data of the popliteus and it is believed that the data of this anatomical research is enlightening to orthopedic surgeons particularly in the field of arthroscopic and plastic surgery. The data can be considered as the database from our population.

Recent Computational Advances Regarding Amyloid-β and Tau Membrane Interactions in Alzheimer's Disease.

The interactions of amyloid proteins with membranes have been subject to many experimental and computational studies, as these interactions contribute in part to neurodegenerative diseases. In this review, we report on recent simulations that have focused on the adsorption and insertion modes of amyloid-β and tau proteins in membranes. The atomistic-resolution characterization of the conformational changes of these amyloid proteins upon lipid cell membrane and free lipid interactions is of interest to rationally design drugs targeting transient oligomers in Alzheimer's disease.

Noxious deindustrialisation and extractivism: Quintero-Puchuncaví in the international division of labour and noxiousness

ABSTRACT This article examines the paradox of ‘noxious deindustrialisation’ – employment deindustrialisation in areas where significantly noxious industries are still operating – in the context of an extractivist economy in the Global South. Analysing the copper-centred industrial area of Quintero-Puchuncaví (Chile), we argue that a key element shaping patterns of local noxious deindustrialisation is the mode of insertion of the affected area in the international division of labour and noxiousness. The latter refers to the global socioecological hierarchy constituted by interconnected differentials in technological capabilities, wage levels, and environmental degradation. In countries marked by extractivism, the simple closure of polluting industries deepens the dependence of their economies on rent-bearing primary product exports, while the permanence of such industries often results in the perpetuation of ‘sacrifice zones’, where communities are exposed to severe noxiousness while receiving only meagre benefits in return. In our view, tackling the ecological crisis requires a more balanced international division of labour, as a step towards decommodifying production and nature. Without transformations in this direction, the current attempts at an energy transition risk provoking an unsustainable deepening of extractivism and inequality.

China’s Insertion in the International Patent Regime: Shaking the Rules Widens the Development Policy Space

Much has been written about the effects of China’s rising role in global economic governance, but the consequences for the development policy space have received little attention. This article examines whether China’s mode of insertion in the international patent regime widens the restricted policy space of its developing country partners to shape their national patent system in a way that facilitates technical progress. Results show that China adopts a minimalist interpretation of the Trade-Related Aspects of Intellectual Property Rights (TRIPS) Agreement as it incorporates its flexibilities at the bilateral and national levels, thus preserving and potentially widening its partners’ development policy space.

Detailed microstructure analysis through monomeric insertion modes of poly(propylene-co-norbornene) copolymers and poly(propylene-co-ethylene-co-norbornene) terpolymers with low norbornene contents

Polypropylene-based thermoplastic elastomers continue to be materials of enormous industrial interest because of their high mechanical performance and durability. These materials are especially important, for instance, in the automotive industry, where these polyolefins accounts for up to 32% of the total plastic used in a car. In the search for new grades, poly(propylene-co-ethylene-co-norbornene) terpolymers might be of interest for the production of a new class of thermoplastic elastomers, whose properties could be tailored by controlling the composition, as well as its distribution. Unfortunately, thorough characterization of these materials has not been possible so far, because of the complexity of their 13CNMR spectra. In this work, two series of poly(propylene-co-norbornene) copolymers and poly(propylene-co-ethylene-co-norbornene) terpolymers, containing low molar fractions of norbornene and ethylene-norbornene, respectively, have been synthesized and characterized in detail by NMR analysis. The comparison of 13CNMR spectra in both series and their respective changes with norbornene ratio in the feed enables a complete assignment of signals, which reveals that the terpolymers are featured by a well-defined microstructure, consisting of propylene sequences bound by both PEP triads and well-defined norbornene-ethylene sequences. Such a particular distribution results from an associated insertion of the ethylene and the norbornene that, in addition, accounts for the higher contents of both comonomers, when compared with their respective copolymers, obtained under the same reaction conditions.

Aggregation‐enabled alkene insertion into carbon–halogen bonds

AbstractMolecular aggregation affects the electronic interactions between molecules and has emerged as a powerful tool in material science. Aggregate effect finds wide applications in the research of new physical phenomena; however, its value for chemical reaction development has been far less explored. Herein, we report the development of aggregation‐enabled alkene insertion into carbon–halogen bonds. The spontaneous cleavage of C–X (X = Cl, Br, or I) bonds generates an intimate ion pair, which can be quickly captured by alkenes in an aggregated state. Additional catalysts or promoters are not necessary under such circumstances, and solvent quenching experiments indicate that the aggregated state is critical for achieving such sequences. The ionic insertion mode is supported by mechanistic studies, density functional theory calculations, and symmetry‐adapted perturbation theory analysis. Results also show that the non‐aggregated state may quench the transition state and terminate the insertion process.

Role of Lipid Packing Defects in Determining Membrane Interactions of Antimicrobial Polymers.

Understanding the emergence and role of lipid packing defects in the detection and subsequent partitioning of antimicrobial agents into bacterial membranes is essential for gaining insights into general antimicrobial mechanisms. Herein, using methacrylate polymers as a model platform, we investigate the effects of inclusion of various functional groups in the biomimetic antimicrobial polymer design on the aspects of lipid packing defects in model bacterial membranes. Two antimicrobial polymers are considered: ternary polymers composed of cationic, hydrophobic, and polar moieties and binary polymers with only cationic and hydrophobic moieties. We find that differing modes of insertion of these two polymers lead to different packing defects in the bacterial membrane. While insertion of both binary and ternary polymers leads to an enhanced number of deep defects in the upper leaflet, shallow defects are moderately enhanced upon interaction with ternary polymers only. We provide conclusive evidence that insertion of antimicrobial polymers in bacterial membrane is preceded by sensing of interfacial lipid packing defects. Our simulation results show that the hydrophobic groups are inserted at a single colocalized deep defect site for both binary and ternary polymers. However, the presence of polar groups in the ternary polymers use the shallow defects close to the lipid-water interface, in addition, to insert into the membrane, which leads to a more folded conformation of the ternary polymer in the membrane environment, and hence a different membrane partitioning mechanism compared to the binary polymer, which acquires an amphiphilic conformation.

Oxidative cyclopalladation triggers the hydroalkylation of alkynes

This report describes the oxidative cyclopalladation activation of a CC bond during the Pd-catalyzed hydroalkylation of alkynes and analyzes potential reaction pathways based on density functional theory calculations. The more favorable pathway in-volves an oxidative cyclopalladation to generate a palladacyclopropene intermediate, which is rarely examined in Pd-catalyzed alkyne transformations. The reaction pathway proposed herein is kinetically favorable relative to the commonly proposed alkyne insertion mode. Furthermore, the Laplacians of the electron density, interaction region indicators, Mayer bond orders, and localized orbital bonding are evaluated to determine the reaction processes and characterize the key intermediates. Theoretical calculations indicate covalent bonding between a Pd(II) center and the two C-atoms in three-membered palladacycle species. Finally, electrostatic potential analysis reveals that the regioselectivity is governed by the charge distribution on the palladacycle moiety during the protonation step.

The T-DNA integration characteristics of monokaryotic mutant library in Lentinula edodes

At present, proteomic and transcriptomic analysis are generally used in the study of gene function of edible fungi, and there are few reports of gene function using mutant library. In this study, the Agrobacterium tumefaciens-mediated transformation (ATMT) system of monokaryotic Lentinula edodes was used for constructing a T-DNA insertional mutant library, and over 800 transgenic strains were collected. Forty-eight strains were randomly selected for T-DNA integration characteristic analysis by whole genome resequencing, and the insertion and integration model of T-DNA in L. edodes were presented. These results lay a foundation for gene function research of L. edodes, and can make it better to understand the insertion and integration mode of exogenous T-DNA in basidiomycetes. By screening the mutant library, 38 mutants with altered thermotolerance were screened out from 221 mutants. According the results of whole genome resequencing, the T-DNA flanking sequences in eight thermotolerancechanged mutants were analyzed and annotated. The further studies for these genes disrupted T-DNA insertion would reveal the molecular mechanisms of high temperature response of L. edodes.