Insemination Of Females Research Articles

ЭФФЕКТИВНОСТЬ ПРИМЕНЕНИЯ НОВОКАИНОВОЙ БЛОКАДЫ ПО И.П. ЛИПОВЦЕВУ ДЛЯ ЛЕЧЕНИЯ КОРОВ ПРИ СУБКЛИНИЧЕСКОМ ЭНДОМЕТРИТЕ

The aim of the study is to investigate the effectiveness of using the middle uterine artery puncture according to I.P. Lipovtsev with the introduction of 0.5 % novocaine solution in combination with the antibio¬tic-containing drug Ceftonit Forte in cows with subclinical endometritis. Objectives: to study the therapeutic effectiveness of the introduction of 0.5 % novocaine solution in combination with the antibiotic-containing drug Ceftonit Forte into the middle uterine artery at different times of the estrous cycle; to establish the fertilization rate of cows after the combined use of novocaine blockade according to I.P. Lipovtsev and the drug Ceftonit Forte at different times of the estrous cycle. The object of the study were cows of the red-motley breed with a subclinical form of endometritis. The diagnosis of subclinical endometritis was made according to the method of N.A. Flegmatov. Laboratory studies have established the presence of subclinical endometritis in 29.24–38.21 % of infertile cows. In 16.98–21.69 % of cases, the cervicovaginal mucus did not have visible threadlike or point inclusions of purulent exudate, but the reaction of such mucus with sperm was positive. Therefore, the absence of purulent exudate inclusions in the cervicovaginal mucus cannot indicate the absence of subclinical endometritis in cows. It has been established that a single use of novocaine blockade according to I.P. Lipovtsev in combination with the antibiotic-containing drug Ceftonit Forte of domestic production, regardless of the timing of administration during the estrous cycle, ensures recovery of 95.24–100 % of cows, and the fertilization rate after artificial insemination of females is 76.19–80.95 %. In addition, there is no need to wait for the onset of estrus in cows with subclinical endometritis to administer drugs into the cervical canal, as is generally accepted in veterinary practice, which allows for more rapid treatment of animals with this uterine pathology and significantly reduces the number of days of infertility.

Boric acid toxic sugar bait suppresses male Aedes aegypti (Diptera: Culicidae): wing beat frequency and amplitude, flight activity, fecundity, insemination, and mate-finding Allee effect.

Controlling the spread of arboviral diseases remains a considerable challenge due to the rapid development of insecticide resistance in Aedes mosquitoes. This study evaluated the effects of boric acid-containing toxic sugar bait (TSB) on field populations of resistant Aedes aegypti mosquitoes. In addition, this study examined the flight activity and wing beat frequency and amplitude of males and the flight activity, fecundity, and insemination of females after pairing with males exposed to TSB. The population dynamics of Aedes mosquitoes under imbalanced sex ratios were examined to simulate realistic field conditions for male suppression under the effect of TSB. The mortality of male mosquitoes was consistently high within 24 h after exposure. By contrast, the mortality of female mosquitoes was inconsistent, with over 70% mortality observed at 168 h. The flight activity and wing beat amplitude of treated males were significantly lower than those of controls, but no significant difference in wing beat frequency was detected. The fecundity and insemination of treated female mosquitoes were lower than those of controls. A simulation study indicated that considerably low male population densities led to mating failures, triggering a mate-finding Allee effect and resulting in persistently low population levels. Boric acid-containing TSB could effectively complement current chemical intervention approaches to control resistant mosquito populations. TSB is effective in reducing field male populations and impairing male flight activity and female-seeking behavior, resulting in decreased fecundity and insemination. Male suppression due to TSB potentially results in a small mosquito population. © 2024 Society of Chemical Industry.

Factors influencing pregnancy per artificial insemination (AI) and embryonic mortality in Nelore females subjected to timed-AI in Brazil

A greater understanding of factors influencing fertility is essential to improve pregnancy rates and reduce the occurrence of embryonic mortality in beef herds. The objective of the current study was to evaluate retrospective data of pregnancy per artificial insemination (P/AI) and pregnancy loss in Nelore females subjected to timed-AI (TAI) in Brazil. Data from 40,104 TAI collected from six breeding seasons (2016–2022) were analyzed, and the effects of animal category (e.g., classification based on age and parity), farm, month of parturition, sire, sire breed (Nelore vs Angus), estrus expression at TAI, animal temperament, and body condition scores (BCS) were evaluated. P/AI and pregnancy loss were affected (P < 0.001) by animal category. There was also an effect of farm (P = 0.0013) on P/AI and pregnancy loss (P = 0.001), as P/AI ranged from 49.28% and 55.58% and pregnancy loss from 3.37% to 6.89% across the herds evaluated. Month of parturition also affected (P < 0.001) P/AI and was higher for cows that became pregnant at the beginning of the previous breeding season. Calmer animals, presenting lower velocity scores while exiting the chute following TAI, achieved higher P/AI (P < 0.001). Lower BCS at TAI was associated (P < 0.001) with increased pregnancy loss, and BCS gain following AI was associated (P < 0.001) with reduced rates of embryonic mortality. There was a major effect (P < 0.001) of sire on P/AI and pregnancy loss, as P/AI ranged from 11% to 79%, and embryonic mortality from 0% to 40% for the bulls used in the study, highlighting the importance of the sire fertility on overall pregnancy success. Results from the current study reinforce the idea that animal age and parity at the beginning of the breeding season, BCS at the onset of estrous synchronization, BCS gain following AI, estrus expression at TAI, sire, and month of parturition are important factors influencing P/AI and rates of embryonic mortality in beef herds.

Результаты использования самцов нового породного типа серебристо-черных лисиц «Савватьевский» в зверохозяйстве «Вятка»

The article presents the results of the use of males of the new breed type «Savvatievsky» on the females of the Vyatka fur farm. The influence of the type and frequency of insemination of Vyatka females by «Savvatiev» and Vyatka males on reproduction indicators was studied. The influence of the date of insemination on fertility, business output, % of nesting, the number of unfavorably foaled females, % of the young when inseminated by males of different breed types was determined. A comparative analysis of the indicators of Vyatka females when covered with different breed types of males was carried out. It was revealed that Vyatka females inseminated by one male twice with the use of both «Savvatievsky» and Vyatka males have the best reproduction results. The best indicators are females inseminated in the period 21 – 1. by «Savvatievsky» males and 1 – 10. by the Vyatka fur farm. The conducted studies have shown that "Savvati" males, when covering Vyatka females in 2014, have the best reproduction results, fertility is 2 heads higher, and business output is 2,52 heads higher than in females of sperm-inseminated males of the Vyatka type of foxes, the difference is statistically significant.

Characterizing Physical Interactions between Male and Female Mosquitoes (Aedes aegypti) in Relation to Female Receptivity and Insemination Outcomes Using a Hydrophobic Fluorescent Dye.

Aedes aegypti, the yellow fever mosquito, presents a major threat to human health across the globe as a vector of disease-causing pathogens. Females of this species generally mate only once. From this single mating event, the female stores sufficient sperm to fertilize the multiple clutches of eggs produced during her lifetime. Mating causes dramatic changes in the female's behavior and physiology, including a lifetime suppression of her mating receptivity. Female rejection behaviors include male avoidance, abdominal twisting, wing-flicking, kicking, and not opening vaginal plates or extruding the ovipositor. Many of these events occur on a scale that is too miniscule or fast to see by eye, so high-resolution videography has been used to observe these behaviors instead. However, videography can be labor intensive, require specialized equipment, and often requires restrained animals. We used an efficient, low-cost method to record physical contact between males and females during attempted and successful mating, determined by recording spermathecal filling after dissection. A hydrophobic oil-based fluorescent dye can be applied to the abdominal tip of one animal and can be subsequently transferred to the genitalia of animals of the opposite sex when genital contact occurs. Our data indicate that male mosquitoes make high levels of contact with both receptive and unreceptive females and that males attempt to mate with more females than they successfully inseminate. Female mosquitoes with disrupted remating suppression mate with and produce offspring from multiple males, transferring dye to each. These data suggest that physical copulatory interactions occur independently of the female's receptivity to mate and that many of these interactions represent unsuccessful mating attempts that do not result in insemination.

IN MEMORY OF HRYHORII SEMENOVYCH SHARAPA (1933–2022) – A RECOGNIZED UKRAINIAN SCIENTIST IN THE FIELD OF FARM ANIMAL REPRODUCTION

The purpose of the article is to highlight the life path and creative pursuits of Candidate of Biological Sciences, Honored Worker of Agriculture of Ukraine H. S. Sharapa in the field of reproductive biotechnology of the farm animals and improvement of artificial insemination techniques. Research methods are general scientific (analysis, synthesis, classification), special historical (problematic-chronological, comparative-historical, biographical), source studies. The scientific novelty of the article lies in the systematization of the scientific heritage of the scientist and the generalization of creative achievements that have found wide practical application in the field of animal husbandry. It is substantiated that H. S. Sharapa has made a significant contribution to the study of the anatomical and physiological characteristics of the genital organs of cows, sheep and bulls of domestic breeds of cattle, to the improvement of the technique of insemination of females. Scientists have developed effective methods of increasing the reproductive capacity of breeding animals, optimal modes of their sexual load, modes of conservation and use of sperm, technology of artificial insemination of cows and heifers; a number of diagnostic, preventive and treatment measures for gynecological diseases were proposed, which contributed to the increase in the profitability of the production of livestock products in Ukraine. H. S. Sharapa made efforts to organize research work in animal husbandry as the Head of the Laboratory of Breeding Biology of Farm Animals of the Kyiv Research Station of Animal Husbandry "Terezyne" and relevant departments of the Institute of Animal Breeding and Genetics named after M.V.Zubets of NAAS. The creative approaches of the scientist in the field of spreading zootechnical education and popularizing the scientific achievements of Ukrainian scientists in the field of reproductive biotechnology are summarized.

The tribes Kampimodromini Kolodochka and Typhlodromipsini Chant and McMurtry (Mesostigmata: Phytoseiidae) in the Serra do Espinhaço, Brazil, with a key to the Brazilian species of Typhlodromips De Leon

Phytoseiid mites of the tribes Kampimodromini and Typhlodromipsini collected from the Serra do Espinhaço (Espinhaço range) are reported here. Two species are proposed as new, Neoparaphytoseius moraesi sp. nov. and Typhlodromips ovametapodalis sp. nov. Typhlodromips constrictatus (El-Banhawy) is redescribed in detail including the first description of the male and the female insemination apparatus. The redescription of the female and the first description of the male and deutonymph of Proprioseius gibbus Moraes and Denmark are given. Complementary descriptions of the females of T. ariri Gondim Jr. and Moraes and T. japi Lofego, Demite and Feres are also included. A key is provided for the separation of the Brazilian species of the genus Typhlodromips.

The Impact of Assisted Hatching on Monozygotic Twinning is Related to Female Age and Insemination Method: A New Perspective - CORRIGENDUM.

The Impact of Assisted Hatching on Monozygotic Twinning is Related to Female Age and Insemination Method: A New Perspective - CORRIGENDUM.

The insemination status and social context influences quail laying and social behavior: A novel experimental set up

Japanese quails in wild life live in small groups with females being even solitary during the laying period. Although it is a poultry species widely used for egg production, information regarding laying behavior motivations or influencing variables is scarce. Our study focuses on evaluating along 7 d the quail laying behavior in a novel environmental set up. This set up allows the female to choose between remaining separated from a conspecific in one side of the apparatus or to voluntarily enter their space (box-mate side) and interact with it. We evaluated whether the female insemination status prior to enter the environmental set up, and the presence of a female or a male partner in the box-mate side can influence their laying and social behavior. Thus, 4 experimental groups were established. Females spent a higher (P < 0.05) percentage of time in the box-mate side than in their separated sector in all groups. In 3 of the 4 experimental groups (non-inseminated females interacting with a female or a male box-mate, and inseminated females interacting with a male box-mate) females also laid a greater percentage (≥65%, P < 0.05, in all cases) of eggs in the box-mate sector than in their separated sector. However, the group of inseminated females that interacted with a female box-mate shifted their egg distribution and laid equally between both sides of the apparatus. Aggressive social interactions were reduced (P < 0.05) throughout the testing days but this was depending upon the female insemination status and the sex of their box-mate. Findings suggest that females can change their laying side choice when they are inseminated but depending on the sex identity of their box-mate partners. Thus, providing quail female breeders with the option of laying their eggs in separated enclosures from conspecifics could be key to favor their well-being.

The Impact of Assisted Hatching on Monozygotic Twinning is Related to Female Age and Insemination Method: A New Perspective.

Whether assisted hatching (AH) is associated with a higher incidence of monozygotic twinning (MZT) in women undergoing assisted reproductive technology remains controversial; the aim of the study was to demonstrate the relationship between AH and MZT. A total of 8900 clinical pregnancies were selected among embryo transfer cycles from January 2011 to October 2019. Women receiving day (D) 3 embryos were divided into groups A-C: group A (n = 1651) and group B (n = 1045) included women aged ≤37 or ≥38 years, respectively, with zona pellucida (ZP) thinning; group C (n = 3865) included women aged ≤37 years without AH. Women aged ≤37 years who underwent blastocyst transfer and/or blastocyst ZP breaching were included in group D (n = 2339). The incidence of MZT was compared among groups A, B and C, and between groups C and D. The incidence of MZT in group B (2.2%) was significantly higher than in group A (1.0%), especially following intracytoplasmic sperm injection (ICSI), while the incidence of MZT in group A (1.0%) was significantly lower than in group C (2.2%). The MZT rate with invitro fertilization was higher in group D (2.8%) than in group C (2.2%), but the MZT rate following ICSI was not significantly different between the two groups. ZP thinning of D3 embryos may increase the risk of MZT in older women (≥38 years), but decrease it in younger women (≤37 years). ZP breaching may be useful to reduce the incidence of MZT in ICSI-generated blastocysts.

Effect of Blood Homocysteine on the Outcome of Artificial Insemination in Women with Polycystic Ovary Syndrome.

Background Polycystic ovary syndrome (PCOS) is the major reason for women's low fertility and the most frequent endocrine disorder in women of childbearing age. Homocysteine is an amino acid that contains sulfur that has been negatively correlated with the reproductive outcome of polycystic women treated with IVF/ICSI. However, the impact of blood homocysteine levels on the outcome of artificial insemination in polycystic ovary syndrome women is unknown. The goal of this study is to examine the impact of serum homocysteine on the result of intrauterine insemination in females who have polycystic ovary syndrome (PCOS). Methods 96 infertile women (129 cycles) treated with artificial insemination were collected, including 66 cases (87 cycles) in the case group (PCOS group) and 30 cases (42 cycles) in the control group (male factor infertility). The differences in general data amongst two groups, such as BMI, Hcy, and age, were compared. The case group has been classified into two groups based on serum Hcy level: LHcy group (Hcy < 15) and HHcy group (Hcy ≥ 15). The relationship among pregnancy and serum Hcy level outcome in PCOS women was compared. Results The PCOS group had substantially increased serum homocysteine levels in comparison to the control group (P = 0.019). Among PCOS women, the clinical pregnancy rates of artificial insemination in the HHcy group and LHcy group were 14.29% and 37.88%. The difference among the two groups was substantial (P = 0.044). Artificial insemination frequency, ovulation induction, BMI, infertility years, AMH, serum testosterone, HOME IR, TSH, TPOAb, hCG, daily follicle size, intimal thickness, and other factors did not differ greatly between the two groups. Conclusion Serum homocysteine levels are increased in women having PCOS. Their levels above the threshold will lower the clinical pregnancy rate of intrauterine insemination in PCOS women.

P-551 High concordance of the embryonic cell-free DNA with the inner cell mass: impact of blastocyst quality, patient age and mode of fertilization

Abstract Study question Does the embryonic cell-free DNA (cfDNA) in the culture medium represent the chromosomal content of the inner cell mass (ICM)? Which factors impact concordance rates? Summary answer There is high ploidy concordance between ICM biopsies and embryonic cfDNA. This value is independent of female age, insemination technique and embryo quality. What is known already The existence of embryonic cfDNA in spent blastocyst medium (SBM) has been confirmed in recent studies, opening a new era of possibilities for non-invasive preimplantation genetic testing for aneuploidy (niPGT-A). High concordance rates of cfDNA with trophectoderm (TE) biopsies and with whole blastocysts have been reported. However, the compartment(s) from where this DNA originates remain unclear. Both TE and ICM are potential sources, but, at the moment, the origin of this cfDNA is unknown as well as the mechanisms underlying its secretion into the medium. Study design, size, duration We carried out a prospective study to investigate the concordance of cfDNA with the corresponding TE and ICM biopsies. 141 day-6/7 blastocysts were donated for research after written informed consent signature for the project approved by the Ethics Committee. Embryos underwent TE biopsy and SBM collection in the same PGT-A cycle. ICM biopsy in thawed blastocysts was performed after TE biopsy diagnosis. cfDNA, TE and ICM biopsies were analyzed from January 2019 to November 2021. Participants/materials, setting, methods Embryos were cultured in routine conditions up to day 4. They were then washed and transferred to a new 10μl culture medium droplet. On day 6, SBM was collected and frozen at -20 °C; and blastocyst biopsy and vitrification were performed. Subsequently, blastocysts were thawed and ICM biopsy was conducted. All samples were analyzed by NGS (Ion ReproSeq PGS kit, ThermoFisher Scientific) and the results were analyzed with customized algorithms for TE, ICM and cfDNA. Main results and the role of chance In combination, the three sample types (cfDNA, ICM and TE) were informative in 81.6% of the blastocysts (115/141). Considering the ICM as the reference, ploidy concordance (i.e. being both euploid or aneuploid) for cfDNA was 86.1% (99/115) and for TE was 89.6% (103/115), without statistical difference. False positive rates were similar for cfDNA and for TE biopsies (6.1% and 9.6%, respectively), and false negative rates were not significantly different, but higher in cfDNA (7.8%) than in TE (0.9%), due to potential contamination with maternal DNA. Ploidy concordance between embryo cfDNA and TE biopsies was 89.6% (103/115). When the results were stratified by female age (≤37 or &amp;gt; 37 years), insemination technique (ICSI or IVF), blastocyst expansion degree (expanded, hatching or fully hatched), and ICM/TE quality (A or B), the informativity of the cfDNA was very similar between the different groups and ranged from 83.7% to 100%. Nevertheless, there were subtle differences for ICM-cfDNA ploidy concordance. It was slightly increased for the older female age group (88.3% vs 83.6% female age ≤37) as well as for ICSI (89.7% vs 82.5% in IVF) and for ICM quality B (88.4% vs 80.0% for ICM A). None of those differences reached statistical significance. Limitations, reasons for caution When stratifying according to the different criteria, the sample size analyzed was too small to draw strong conclusions. Therefore, more studies, with bigger sample size, are needed to replicate the results. Wider implications of the findings The embryonic cfDNA released to the culture medium provides information of the overall blastocyst chromosomal constitution, as suggested by the high ploidy concordance rates reported between ICM and cfDNA. This supports the use of niPGT-A as an alternative to other invasive aneuploidy detection methods that require a biopsy. Trial registration number ClinicalTrials.gov. ID NCT03520933

VOLODYMYR FRANKOVYCH STAKHOVSKYI – RECOGNIZED AUTHORITY IN ANIMAL REPRODUCTION

VOLODYMYR FRANKOVYCH STAKHOVSKYI – RECOGNIZED AUTHORITY IN ANIMAL REPRODUCTION

Damage and spatiotemporal dynamics of the Ngaio flat mite, Brevipalpus ferraguti (Trombidiformes: Tenuipalpidae), with observations on the development of the female insemination system

We studied the Ngaio flat mite, Brevipalpus ferraguti Ochoa & Beard, on Myoporum laetum (Scrophulariaceae), a common introduced plant used as hedgerows in gardens and green areas of the Mediterranean, where the mite causes considerable damage. We first describe the damage, and then the patterns of mite seasonal abundance and spatial distribution. Finally, we address the development of the female insemination system at the population level. Damage occurs on both sides of the leaves, starting with a uniform stippling and bronzing and ending in the leaves drying out and extensive defoliation that coincides with summer. Mite population peaked between June and August, maintained moderate levels in autumn and winter and reached its lowest density in early spring. Active motile immatures and eggs were present throughout the year. Females and motile immature forms were more abundant on the abaxial (lower) leaf surface, but eggs were deposited on both surfaces indistinctly, suggesting that females actively move to the adaxial (upper) surface in summer to oviposit. All the developmental stages were aggregated on the leaves throughout the year regardless of their population density. Our study suggests that a binomial or presence-absence sampling, examining only the number of females on the abaxial surface, can accurately estimate the total mite density levels. Only 23.5% of females possessed a fully developed spermatheca, whereas in 76.5% of the cases the seminal receptacle was not present or not developed. Females with a complete spermatheca were less abundant in summer. Average temperatures and host plant species affected the occurrence of this reproductive structure.

Swarming Behavior in Anopheles gambiae (sensu lato): Current Knowledge and Future Outlook.

Effective management of insect disease vectors requires a detailed understanding of their ecology and behavior. In Anopheles gambiae sensu lato (s.l.) (Diptera: Culicidae) mating occurs during swarming, but knowledge of their mating behavior under natural conditions is limited. Mosquitoes mate in flight over specific landmarks, known as swarm markers, at particular locations. Swarms consist of males; the females usually approach the swarm and depart following copulation. The number of mating pairs per swarm is closely associated with swarm size. The shape and height of swarm markers vary and may depend on the environmental conditions at the swarm’s location. Male–male interactions in mosquito swarms with similar levels of attractive flight activity can offer a mating advantage to some individuals. Flight tone is used by mosquitoes to recognize the other sex and choose a desirable mate. Clarifying these and other aspects of mosquito reproductive behavior can facilitate the development of population control measures that target swarming sites. This review describes what is currently known about swarming behavior in Anopheles gambiae s.l., including swarm characteristics; mating within and outside of swarms, insemination in females, and factors affecting and stimulating swarming.

Artificial insemination and parthenogenesis in the whitespotted bamboo shark Chiloscyllium plagiosum

Non-lethal methods for semen collection from elasmobranchs to better understand species reproduction has accompanied the development of artificial insemination. Ejaculates (n = 82) collected from whitespotted bamboo sharks Chiloscyllium plagiosum (n = 19) were assessed and cold-stored raw or extended at 4 °C. Females (n = 20) were inseminated with fresh or 24–48 h cold-stored raw or extended semen and paternity of offspring determined with microsatellite markers. Insemination of females with fresh semen (n = 10) resulted in 80 hatchlings and 27.6% fertility. Insemination of females with semen cold-stored 24 h (n = 4) and 48 h (n = 1) semen resulted in 17 hatchlings and fertilization rates of 28.1% and 7.1% respectively. Two females inseminated with fresh or cold-stored semen laid eggs that hatched from fertilization and parthenogenesis within the same clutch. Parthenogenesis rate for inseminated females was 0.71%. Results demonstrate artificial insemination with cold-stored semen can provide a strategy for transport of male genetics nationally and internationally, precluding the need to transport sharks. Production of parthenotes in the same clutch as sexually fertilized eggs highlights the prevalence of parthenogenesis in whitespotted bamboo sharks and poses important considerations for population management.

Relationship among serum metabolic hormones with pregnancy rates to fixed-time artificial insemination in Bos indicus beef females

Two experiments compared body parameters and serum concentrations of GH, IGF-I, and leptin in Bos indicus females based on their pregnancy status to fixed-time artificial insemination (FTAI). In Exp. 1, 226 nulliparous heifers were assigned to a puberty induction protocol (d -35 to d -23), followed by an estrus synchronization and FTAI protocol (d -11 to 0). Heifers received a controlled internal progesterone releasing insert (CIDR) previously used three times on d -35, followed by 0.6 mg of estradiol cypionate and CIDR removal on d -23. Heifers received 2 mg of estradiol benzoate and a CIDR previously used twice on d -11, 12.5 mg of dinoprost tromethamine in addition to CIDR removal, 0.6 mg of estradiol cypionate and a 200 IU of eCG on d -2, followed by FTAI on d 0. Heifer BW and BCS were recorded on d -35, -11, and 30, whereas blood samples were collected on d -35, -23, -11, 0, and 30. In Exp. 2, 821 multiparous lactating Nelore beef cows were assigned to the estrus synchronization and FTAI protocol described in Exp. 1, but received 300 IU of eCG on d -2. Cow BW and BCS were recorded on d -11 and 30, and blood samples were collected on d -11, 0, and 30. In both experiments, pregnancy status was verified via transrectal ultrasonography on d 30. In Exp. 1, heifers that became pregnant had greater (P = 0.03) IGF-I concentrations on d 0 and less (P = 0.05) IGF-I concentrations on d 30 compared to heifers that did not become pregnant (pregnancy status × day; P < 0.01). No differences in BW, BCS, GH and leptin were detected (P ≥ 0.23) according to heifer pregnancy status. In Exp. 2, cows that became pregnant had less (P = 0.03) leptin concentrations on d -11, and less (P = 0.01) IGF-I concentrations on d 30 compared to cows that did not become pregnant (pregnancy status × day; P ≤ 0.05). No differences in BW, BCS, and GH were detected (P ≥ 0.15) according to cow pregnancy status. In summary, serum leptin at the beginning of estrus synchronization was negatively associated with pregnancy rates to FTAI in multiparous cows. A negative relationship was also observed between serum IGF-I during early gestation with pregnancy rates to FTAI across parities. These results provide novel insights on the relationships among metabolic hormones with reproductive performance of B. indicus females.

Influence of antral follicle count on in vitro embryo production, sexual precocity and conception rate of Senepol cattle

Context There are contradictory results concerning the relationship between antral follicle count (AFC) and reproductive traits for different breeds of cattle. Understanding this relationship could be useful to selecting animals for breeding. Aims We evaluated the effects of AFC on parameters related to in vitro embryo production (IVP), and the influence of AFC on sexual precocity and conception rate at first fixed timed artificial insemination (FTAI) of Senepol females. Methods Ovarian ultrasound was performed to count antral follicles, and females were classified as low AFC (≤22 follicles), intermediate AFC (23–36 follicles) or high AFC (&gt;36 follicles). Expt 1 evaluated the influence of AFC on IVP, with data from 146 adult cows that were submitted to follicular aspiration. Expt 2 evaluated the influence of AFC on heifer sexual precocity, determined by the presence of a corpus luteum (n = 608), and on conception rate at first FTAI (n = 143). Key results High AFC cows had a greater (P &lt; 0.0001) number of viable oocytes and cleaved embryos and a higher average number of embryos than low AFC cows (38.28 vs 9.92, 38.28 vs 7.75 and 9.34 vs 2.41, respectively). However, the conversion rate (total viable embryos per oocytes) was not affected (P = 0.46) by AFC category. High AFC heifers tended (P = 0.07) to be more sexually precocious than low AFC heifers (16.73% vs 9.47%). An influence of AFC or of AFC category on conception rate at first FTAI was not detected (P = 0.35 and 0.19, respectively). Conclusions Despite greater IVP in high AFC cows, the conversion rate of viable oocytes to IVP was not affected by AFC category. High AFC heifers tended to be more sexually precocious, although AFC did not influence the conception rate at first FTAI. Implications Oocyte quality is probably not influenced by AFC, but selection of animals according to AFC may improve the sexual precocity of the herd.

EFFECTS OF ORAL ADMINISTRATION OF Lepidium sativum, Moringa oleifera OILS AND AQUEOUS EXTRACT OF Vitex agnnus castus ON REPRODUCTIVE PERFORMANCE AND BLOOD BIOCHEMICAL OF DOE RABBITS

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Characterization of the Drosophila suzukii β2-tubulin gene and the utilization of its promoter to monitor sex separation and insemination.

The Drosophila melanogaster β2-tubulin gene (Dm-β2t) controls the function of microtubules in the testis and sperm, and has been evaluated for use in biocontrol strategies based on the sterile insect technique, including sexing and the induction of male sterility. The spotted-wing Drosophila (Drosophila suzukii) is native to eastern Asia but has spread globally as an invasive pest of fruit crops, so biocontrol strategies are urgently required for this species. We therefore isolated the β2tubulin ortholog Ds-β2t from the USA laboratory strain of D. suzukii and confirmed the presence of functional motifs by aligning orthologs from multiple insects. The developmental expression profile of Ds-β2t was determined by RT-PCR using gene-specific primers and was similar to that of Dm-β2t. We then isolated the Ds-β2t promoter and used it to generate transgenic strains expressing a testis-specific fluorescent protein starting from the thirdinstar larvae. Efficient sexing was achieved based on fluorescence detection, and the transgenic males showed a similar survival rate to wild-type males. Fluorescence imaging and PCR were also used to confirm the insemination of wild-type females by transgenic males. We therefore confirm that D. suzukii strains expressing fluorescent markers under the control of the Ds-β2t promoter can be used for sexing and the confirmation of mating, and we discuss the wider potential of the Ds-β2t promoter in the context of genetic control strategies for D. suzukii.