Insecticidal Agents Research Articles

The Efficiency of Aboriginal Entomopathogenic Nematodes from Semi-Arid Zone Against Tenebrionidae Larvae with Comparison to Commercial Bio-Insecticides

Chemical insecticides are intensively used in agriculture which cause negative effects on environmental sustainability by increasing pest tolerance and continuous action on local fauna. In contrast to chemical insecticides, biological insecticides target specific pests, minimizing their impact on the surrounding environment. However, most of the natural insecticides available in the market are expensive for farmers in the Central Asian region. This study is the first evidence of the effectiveness of local entomopathogenic nematodes (EPN) from the arid zone of northern Kazakhstan that can be used as an alternative to export EPN products. The laboratory experiment is conducted with two local and two commercial Steinernema feltiae and Steinernema carpocapsae, which are applied to larvae of Tenebrionidae. The experiment design is randomized with four doses of EPN (100, 200, 300, and 400 IJ/cm2) and a control (without EPN). According to the experiment results, one out of two local EPNs (AF29 at dose 100 IJ/cm2) caused a high mortality rate (67%) among larvae. This finding confirms that local EPNs can compete with commercial EPNs and potentially be used as insecticidal agents.

Larvicidal, antioxidant and biotoxicity assessment of (2-(((2-ethyl-2 methylhexyl)oxy)carbonyl)benzoic acid isolated from Bacillus pumilus against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus.

Mosquitoes are a vector for many dreadful diseases known for their public health concern. The continued use of synthetic insecticides against vector control has led to serious environmental impacts, human health problems, and the development of insect resistance. Hence, alternative mosquito control methods are needed to protect the environment and human health. In the present study, the bioefficacy of (2-(((2-ethyl-2 methylhexyl)oxy)carbonyl) benzoic acid isolated from Bacillus pumilus were tested against Aedes aegypti, Culex quinquefasciatus and Anopheles stephensi. The isolated bioactive compound was characterized through thin layer chromatography (TLC), UV-visible spectroscopy (UV), Fourier-transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and gas chromatography-mass spectrometry analysis. The pure compound caused a high percent mortality rate in a dose-dependent manner, the obtained values were 96, 82, 69, 50 and 34%; 86, 72, 56, 43, and 44%; 100, 90, 83, 70 and 56% against Ae. aegypti, Cx. quinquefasciatus, and An. stephensi respectively. The effective lethal concentration values (LC50) were 13.65, 14.90 and 9.64ppm against Ae. aegypti, Cx. quinquefasciatus, An. Stephensi, respectively. The effect of (2-(((2-ethyl-2 methylhexyl)oxy)carbonyl) benzoic acid significantly increased the superoxide dismutase, catalase, α, β esterase and Glutathione-S-transferase level after 24h of the treatment period. The comet assay confirmed that isolated compound causes DNA damage in all tested insects. Histopathological examinations of treated larvae showed shrunken body posture, damaged epithelial cells and microvillus as compared to control organisms. The biosafety of the isolated compound was assessed against G. affinis and did not produce mortality which confirmed that the activity of the isolated compound is species specific. The current study concludes that the critical success factors of new insecticidal agent development are based on the eco-compatibility and alternative tools for the pesticide producing industry.

Facile Synthesis, Biological Evaluation, DFT Studies and in Silico Prediction ADME/Pharmacokinetics Properties of N-(1-(2-Chlorobenzo[h]Quinolin-3-yl)-1-Substituted-vin-2-yl)Benzamide Derivatives

A new series of various N-(1-(2-chlorobenzo[h]quinolin-3-yl)-1-substituted-vin-2-yl)benzamide derivatives was designed and synthesized based on the 2-chlorobenzo[h]quinoline core as potential antioxidant and insecticidal agents. In vitro antioxidant activities of these compounds were evaluated and compared with commercial antioxidant (ascorbic acid). The results showed good antioxidant activities of these compounds. In addition, in vitro insecticidal activities revealed that most of these compounds exhibited high insecticidal activity against cotton leafworm compared with chlorpyrifos. Some compounds in these series were better than the commercial insecticidal, chlorpyrifos.

Insecticidal Activity of Berberis Royleana: Various Extracts against Callosobruchus Maculatus

The residents of remote areas in developing countries mostly rely on traditional plants to curedifferent ailments. Berberis royleana is a rare species among the genus Berberis whichcommonly comprises 500 species distributed in Nepal, Afghanistan, Siberia, and other parts ofthe world. Berberis are used to make herbal medication to fight against infections, oculartrachoma, AIDS, and diarrhea. The current study was carried out to explore the in vitroinsecticidal activity of B. royleana in various extracts by direct contact application processagainst the insect Callosobruchus maculatus. The maximum mortality rate of 60% was recordedby water fraction, whereas methanolic fraction had 50% mortality, n-Hexane had 40% mortality,while ethyl acetate and chloroform both had 30% mortality at 72 hours. Thus, this studyindicated that from the medicinal point of view this specie is very significant, with a greatpotential for insecticidal activities against C. maculatus. It is further suggested that B. royleanacould be used as a good insecticidal agent against dengue and malarial vectors. In addition, theside effects like toxicity could be addressed to avoid any mishaps.

Essential Oils from Cameroonian Aromatic Plants as Effective Insecticides against Mosquitoes, Houseflies, and Moths.

Recently, spices have attracted the attention of scientists and agrochemical companies for their potential as insecticidal and acaricidal agents, and even as repellents to replace synthetic compounds that are labeled with detrimental impacts on environment and human and animal health. In this framework, the aim of this study was to evaluate the insecticidal potential of the essential oils (EOs) obtained from three Cameroonian aromatic plants, namely Monodora myristica (Gaertn.) Dunal, Xylopia aethiopica (Dunal) A. Rich., and Aframomum citratum (J. Pereira) K. Schum. They were produced by hydrodistillation, with yields of 3.84, 4.89, and 0.85%, respectively. The chemical composition was evaluated by GC-MS analysis. The EOs and their major constituents (i.e., geraniol, sabinene, α-pinene, p-cymene, α-phellandrene, and β-pinene) were tested against the polyphagous moth pest, i.e., Spodoptera littoralis (Boisd.), the common housefly, Musca domestica L., and the filariasis and arbovirus mosquito vector, Culex quinquefasciatus Say. Our results showed that M. myristica and X. aethiopica EOs were the most effective against M. domestica adults, being effective on both males (22.1 µg adult−1) and females (LD50: 29.1 µg adult−1). The M. myristica EO and geraniol showed the highest toxicity on S. littoralis, with LD50(90) values of 29.3 (123.5) and 25.3 (83.2) µg larva−1, respectively. Last, the EOs from M. myristica and X. aethiopica, as well as the major constituents p-cymene and α-phellandrene, were the most toxic against C. quinquefasciatus larvae. The selected EOs may potentially lead to the production of cheap and effective botanical insecticides for African smallholders, although the development of effective formulations, a safety evaluation, and an in-depth study of their efficacy on different insect species are needed.

Hedychium coronariumJ. Koenig: Traditional Uses, Phytochemistry, Biological Activities and Future Aspects

Abstract:Hedychium coronarium J. Koenig belongs to the family Zingiberaceae, generally known as butterfly ginger, butterfly lily, cinnamon jasmine, garland flower and ginger lily. It is a medicinal plant grown throughout India, Southeast Asian countries, China, Japan, and Brazil. Traditionally, it is used as a febrifuge, tonic, anti-rheumatic, used to treat asthma, headache, arthritis, bronchitis, blood diseases, eye diseases, gastric diseases, and many other diseases. H. coronarium contains important secondary metabolites such as alcohols, aldehydes, ketones, esters, oxides, phenolics, flavonoids, saponins, glycosides, labdane diterpenes, and sesquiterpenes. Limonene, myrcene, p-cymene, camphene, γ-terpinene, β-pinene, 1,8-cineole, linalool, α-pinene, and 10-epi-γ-eudesmol have been identified as the main constituents of volatile oils of H. coronarium along with the key elements like trans-meta-mentha-2, 8-diene, linalool, α-terpineol, terpin-4-ol, α-pinene, γ-terpinene, and camphene. Phytochemical studies on various solvent extracts of H. coronarium revealed that the plant holds the diterpenes- coronarin-A-I, isocoronarin-D, pacovatin A; sesquiterpene- (+)-nerolidol, hedychiol A, hedychiol B 8,9-diacetate; sterols- daucosterol, stigmasterol, β-sitosterol and flavonoid- 5-hydroxy-3,7,4’-trimethoxyflavon, chrysin. Coronarin D, a bioactive compound, is reported for anti-cancer activities of H. coronarium. Diterpenes isolated from H. coronarium extracts and essential have been reported as antioxidant, antitumor, antidiabetic, antiproliferative, antihelmintic, mosquitocidal, larvicidal, antilithiatic, chemopreventive, antiophidian, insecticide, antifungal, allelopathic, and antimicrobial agent. Based on the literature, the essential oils, extracts and isolated active compounds of H. coronarium could be used to develop as flavor and fragrance agents, food preservatives, botanical pesticide, neutraceuticals and pharmaceuticals. This review paper aims to go over traditional uses, phytochemical analysis, pharmacological activities, scientific techniques for variety development, conservation, and proper utilization and identify future opportunities for H. coronarium.

Bintaro (Cerbera odollam and Cerbera manghas): an overview of its eco-friendly use, pharmacology, and toxicology.

Bintaro is a tropical mangrove plant often used as a shade tree found in Asia, Australia, Madagascar, and the Islands of the Western Pacific Ocean. The word Bintaro is also often pinned to its closest relative species, the Cerbera odollam. Flower color is one of the distinguishing features between these two species. Human poisoning with the cardiotoxic plant Bintaro is common in Southeast Asia because it bears a fruit that yields a powerful poison that has been used for suicide and homicide, hence it is also called the "Indian suicide tree". The seeds of Bintaro contain Cerberin, a cardiac glycoside toxin of the heart that blocks the calcium ion channels in heart muscles, resulting in disruption of the heartbeat most often fatally. The bio-active compound in the kernels of Bintaro varies due to which plant possesses other properties as well. The plant may also be used for medicinal purposes as it shows many pharmaceutical properties. The seeds of the plant have auspicious anticancer properties through apoptotic activity and the leaf extract of the plant was screened for its antioxidant activities. In addition, it is also used as an insecticide, pesticide, or antifungal agent. This review highlights the Pharmaceutical, toxicological, and environmentally friendly approaches of Bintaro.

Direct and Indirect Effects of Essential Oils for Sustainable Crop Protection.

Plant essential oils (EOs) are gaining interest as biopesticides for crop protection. EOs have been recognized as important ingredients of plant protection products including insecticidal, acaricidal, fungicidal, and nematicidal agents. Considering the growing importance of EOs as active ingredients, the domestication and cultivation of Medicinal and Aromatic Plants (MAPs) to produce chemically stable EOs contributes to species conservation, provides the sustainability of production, and decreases the variations in the active ingredients. In addition to these direct effects on plant pests and diseases, EOs can induce plant defenses (priming effects) resulting in better protection. This aspect is of relevance considering that the EU framework aims to achieve the sustainable use of new plant protection products (PPPs), and since 2020, the use of contaminant PPPs has been prohibited. In this paper, we review the most updated information on the direct plant protection effects of EOs, focusing on their modes of action against insects, fungi, and nematodes, as well as the information available on EOs with plant defense priming effects.

A Chromosome-Scale Genome Assembly of a Helicoverpa zea Strain Resistant to Bacillus thuringiensis Cry1Ac Insecticidal Protein.

Helicoverpa zea (Lepidoptera: Noctuidae) is an insect pest of major cultivated crops in North and South America. The species has adapted to different host plants and developed resistance to several insecticidal agents, including Bacillus thuringiensis (Bt) insecticidal proteins in transgenic cotton and maize. Helicoverpa zea populations persist year-round in tropical and subtropical regions, but seasonal migrations into temperate zones increase the geographic range of associated crop damage. To better understand the genetic basis of these physiological and ecological characteristics, we generated a high-quality chromosome-level assembly for a single H. zea male from Bt-resistant strain, HzStark_Cry1AcR. Hi-C data were used to scaffold an initial 375.2 Mb contig assembly into 30 autosomes and the Z sex chromosome (scaffold N50 = 12.8 Mb and L50 = 14). The scaffolded assembly was error-corrected with a novel pipeline, polishCLR. The mitochondrial genome was assembled through an improved pipeline and annotated. Assessment of this genome assembly indicated 98.8% of the Lepidopteran Benchmark Universal Single-Copy Ortholog set were complete (98.5% as complete single copy). Repetitive elements comprised approximately 29.5% of the assembly with the plurality (11.2%) classified as retroelements. This chromosome-scale reference assembly for H. zea, ilHelZeax1.1, will facilitate future research to evaluate and enhance sustainable crop production practices.

Generation of Human Domain Antibody Fragments as Potential Insecticidal Agents against Helicoverpa armigera by Cadherin-Based Screening.

New insecticidal genes and approaches for pest control are a hot research area. In the present study, we explored a novel strategy for the generation of insecticidal proteins. The midgut cadherin of Helicoverpa armigera (H. armigera) was used as a target to screen materials that have insecticidal activity. After three rounds of panning, the phage-displayed human domain antibody B1F6, which not only binds to the H. armigera cadherin CR9-CR11 but also significantly inhibits Cry1Ac toxins from binding to CR9-CR11, was obtained from a phage-displayed human domain antibody (DAb) library. To better analyze the relevant activity of B1F6, soluble B1F6 protein was expressed by Escherichia coli BL21 (DE3). The cytotoxicity assays demonstrated that soluble B1F6 induced Sf9 cell death when expressing H. armigera cadherin on the cell membrane. The insect bioassay results showed that soluble B1F6 protein (90 μg/cm2) caused 49.5 ± 3.3% H. armigera larvae mortality. The midgut histological results showed that soluble B1F6 caused damage to the midgut epithelium of H. armigera larvae. The present study explored a new strategy and provided a basic material for the generation of new insecticidal materials.

Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis

BackgroundAliivibrio salmonicida is the causative agent of cold-water vibriosis in salmonids (Oncorhynchus mykiss and Salmo salar L.) and gadidae (Gadus morhua L.). Virulence-associated factors that are essential for the full spectrum of A. salmonicida pathogenicity are largely unknown. Chitin-active lytic polysaccharide monooxygenases (LPMOs) have been indicated to play roles in both chitin degradation and virulence in a variety of pathogenic bacteria but are largely unexplored in this context.ResultsIn the present study we investigated the role of LPMOs in the pathogenicity of A. salmonicida LFI238 in Atlantic salmon (Salmo salar L.). In vivo challenge experiments using isogenic deletion mutants of the two LPMOs encoding genes AsLPMO10A and AsLPMO10B, showed that both LPMOs, and in particular AsLPMO10B, were important in the invasive phase of cold-water vibriosis. Crystallographic analysis of the AsLPMO10B AA10 LPMO domain (to 1.4 Å resolution) revealed high structural similarity to viral fusolin, an LPMO known to enhance the virulence of insecticidal agents. Finally, exposure to Atlantic salmon serum resulted in substantial proteome re-organization of the A. salmonicida LPMO deletion variants compared to the wild type strain, indicating the struggle of the bacterium to adapt to the host immune components in the absence of the LPMOs.ConclusionThe present study consolidates the role of LPMOs in virulence and demonstrates that such enzymes may have more than one function.

Green Design, Synthesis, and Molecular Docking Study of Novel Quinoxaline Derivatives with Insecticidal Potential against Aphis craccivora.

An efficient and environmentally friendly method was established for designing novel 3-amino-1,4-dihydroquinoxaline-2-carbonitrile (1) via the reaction of bromomalononitrile and benzene-1,2-diamine under microwave irradiation in an excellent yield (93%). This targeted amino derivative was utilized for the construction of a series of Schiff bases (8–13). A new series of thiazolidinone derivatives (15–20) were synthesized in high yields (89–96%) via treatment of thioglycolic acid with Schiff bases (8–13) under microwave irradiation in high yields (89–96%). Moreover, new pyrimidine derivatives (26–30 and 35–38) were prepared by treatment of compound 1 with arylidenes (21–25) and/or alkylidenemalononitriles (31–34) using piperidine as a basic catalyst under microwave conditions. Based on elemental analyses and spectral data, the structures of the new assembled compounds were determined. The newly synthesized quinoxaline derivatives were screened and studied as an insecticidal agent against Aphis craccivora. The obtained results indicate that compound 16 is the most toxicological agent against nymphs of cowpea aphids (Aphis craccivora) compared to the other synthesized pyrimidine and thiazolidinone derivatives. The molecular docking study of the new quinoxaline derivatives registered that compound 16 had the highest binding score (−10.54 kcal/mol) and the thiazolidinone moiety formed hydrogen bonds with Trp143.

Simple approach for the synthesis of a 1,1'-(ethane-1,2 diylbis(azanediyl))bis(pentan-2-ol): 13C/1H NMR spectroscopy and electrical characterizations

1,1'-(Ethane-1,2-diylbis(azanediyl))bis(pentan-2-ol) a β-aminoalcohol material denoted as 2a has been successfully synthesized. A white highly pure transparent material has been obtained after purification by chromatography techniques. Infrared spectroscopy has been used to identify the −NH and −OH functional groups. 1 H and 13 C NMR spectroscopy has confirmed the proposed structure. Complex Impedance Spectroscopy indicates an insulator behavior of the studied materials in the 313-393°C temperature range. Conductivity within this material is in intragrain contribution and its activation energy is about 1.3 eV. The physical properties of the studied material allow to consider β-amino alcohols as versatile intermediates for many organic compounds in the making of natural and synthetic originated biologically active compounds. They are widely used as β-blockers, insecticidal agents, and chiral auxiliaries.

Successful reversal of a rare case of amitraz poisoning with yohimbine—a case report

BackgroundAmitraz is an insecticidal agent and its poisoning cases are rare. We report the successful treatment of amitraz poisoning using yohimbine as an antidote. There is only a single other case report in literature of usage of yohimbine as antidote to amitraz poisoning in a 3-year-old child.Case presentationA 27-year-old woman presented to the hospital with features of severe amitraz intoxication-respiratory depression and loss of consciousness. Along with supportive management in the intensive care unit, she was administered parenteral yohimbine, which resulted in quick recovery and discharge from hospital.ConclusionsYohimbine may serve as a useful antidote in severe amitraz intoxication. However, the conclusive benefit of yohimbine in human cases of amitraz poisoning is yet to be established and is a potential area of research.

Design, Synthesis, and Biological Evaluation of Novel Osthole-Based Isoxazoline Derivatives as Insecticide Candidates.

Natural products are an abundant and environmentally friendly source for controlling plant pathogens and insect pests. Toward the development of new natural product-based pesticides, here, a series of osthole-based isoxazoline derivatives were prepared by [3 + 2] annulation and evaluated for their insecticidal activities and toxicities. The structures of all osthole-based isoxazoline derivatives were characterized by various spectral analyses, and derivative B13 was further confirmed by X-ray crystallography. Among all the osthole derivatives, B2 displayed the most promising growth inhibitory effect on Mythimna separata with a final corrected mortality rate of 96.4% ± 3.3, which was 1.80 times higher than those of both osthole and toosendanin. Derivative B13 displayed the most promising larvicidal activity against Plutella xylostella with an LC50 value of 0.220 mg/mL, which was superior to rotenone. Furthermore, both B13 and B21 also exhibited better control efficacy against P. xylostella than rotenone in the pot experiments. Additionally, the toxicity evaluation suggested that these osthole-based isoxazoline derivatives showed relatively low toxicity toward nontarget organisms. Given these results, osthole derivatives B2, B13, and B21 could be deeply developed as natural insecticidal agents in agriculture.

Discovery of Indoloazepinone Analogues as Novel Antiviral, Antiphytopathogenic Fungus, and Insecticidal Agents

Discovery of Indoloazepinone Analogues as Novel Antiviral, Antiphytopathogenic Fungus, and Insecticidal Agents

Synthesis of paeonol ester derivatives and their insecticidal, nematicidal, and anti-oomycete activities.

Paeonol is extracted and isolated as a rich and sustainable natural bioresource from the root bark of Paeonia suffruticosa, the derivatives of which exhibit numerous biological activities. It is well known that ester compounds play a very important role in pest control, such as organophosphorus, carbamate and pyrethroid pesticides. To discover biorational natural product-based pesticides, three series of (60) paeonol ester derivatives (7a-t, 8g,p, 9g,p, 10g-j,n-u, 11g,u, 12g,u, 13a-p, 14b,c, and 15b,c) were prepared by structural modification of paeonol, and their structures were well characterized by proton nuclear magnetic resonance (1 H-NMR), carbon-13 nuclear magnetic resonance (13 C-NMR), high-resolution mass spectrometry (HRMS), and melting point. Furthermore, we assessed the compounds as insecticidal, nematicidal, and anti-oomycete agents against three serious agricultural pests, Mythimna separata, Heterodera glycines, and Phytophthora capsici. Among all tested compounds: (i) compound 8p showed more significant insecticidal activity than toosendanin, and the final mortality rates of 8p and toosendanin against M. separata (1 mg mL-1 ) were 70.4%, and 51.9%, respectively; (ii) compound 7a exhibited more promising nematicidal activity than paeonol, and the median lethal concentration (LC50 ) values of 7a and 1 against H. glycines were 15.47 and 50.80 mg L-1 , respectively; (iii) compounds 7n and 13m displayed more significant anti-oomycete activity compared to zoxamide against Phytophthora capsici, and the median effective concentration (EC50 ) values of 7n, 13m, and zoxamide were 23.72, 24.51, and 26.87 mg L-1 , respectively; and the protective effect of the compounds against Phytophthora capsici in vivo further confirmed the effectiveness of the agents. This study suggested that the introduction of a nitro at the C5 or C3 position of paeonol could improve its bioactivity against M. separata, H. glycines, and Phytophthora capsici. © 2022 Society of Chemical Industry.

The Valorization of Spent Coffee Ground Extract as a Prospective Insecticidal Agent against Some Main Key Pests of Phaseolus vulgaris in the Laboratory and Field.

The exploitation of massive amounts of food and agro-waste represents a severe social, economic, and environmental issue. Under the growing demand for food products that are free of toxic synthetic insecticides, a methanolic extract of spent coffee grounds (SCGs), which represent the main byproduct of coffee production, was applied in the current study as a bioinsecticide against the main pests of the green bean: Spodoptera littoralis, Agrotis ipsilon, Bemisia tabaci, Empoasca fabae, and Aphis craccivora. A deterrent assay, contact bioassay, and lethal concentration analysis were performed to reveal the repellent, antifeedant, and oviposition deterrent effects. Parallel to the above-mentioned bioassays, the phytochemical composition of the methanolic SCG extract was investigated via a high-performance liquid chromatography (HPLC) analysis. Fourteen phenolic acids and five flavonoids, in addition to caffeine (alkaloid), were identified in the extract. Cinnamic, rosmarinic, and gallic acids were the predominant phenolics, while apigenin-7-glucoside was the main flavonoid, followed by naringin, catechin, and epicatechin. The extract of SCGs showed an insecticidal effect, with a mortality between 27.5 and 76% compared to the control (7.4%) and based on the concentration of the extract used. In the same trend, the oviposition efficiency revealed different batches of laid eggs (0.67, 2.33, 7.33, and 8.67 batches/jar) for 100, 50, and 25% of the SCG extract and the control. Finally, the major components of the SCG extract were docked into the insecticide acetylcholinesterase enzyme to explore their potential for inhibition, where apigenin-7-glucoside showed a higher binding affinity, followed by catechin, compared to the control (lannate). The obtained findings could be a starting point for developing novel bioinsecticides from SCGs.

Front Cover Image, Volume 78, Issue 5

The cover image is based on the Research Article Synthesis and evaluation of novel xanthine‐acrylamides and xanthine‐acrylates as insecticidal agents by Shuyun Zhang et al., https://doi.org/10.1002/ps.6834.image

Study of the effect of daily exposure to an electric insecticide vaporizer on the development of the body of white rats

Introduction. Electric insecticide vaporizer is a heating device, powered by the mains, when turned on this, the insecticidal agent, including the active substance, evaporates into the air of the treated room. The population uses an electric insecticide vaporizer during the warm season to destroy flying blood-sucking insects, which, in addition to epidemiological danger, creates psychological discomfort at night. Highly volatile pyrethroids are used as active ingredients in insecticidal electric vaporizer agents, which have an adverse effect on the nervous system, liver and urinary system. It is also becoming urgent the problem of air pollution indoor, especially for children. Materials and methods. Toxicological methods for assessing the danger of using insecticidal agents; biochemical methods for the study of blood serum. Results. There was studied a long-term inhalation effect of an electric insecticide vaporizer based on transfluthrin on the functional state of white rats. The agent was established to have an effect on the number of eosinophils in the blood, indicating to the development of allergic responses in the body of rats; affects the function of the nervous system, which confirms the literature data; disrupts metabolic processes in the liver, manifested in a change in individual indicators (carbohydrate, protein metabolism, creatinine synthesis). Limitations. In our study, there are no data on the study of organ tissues, nor have we studied the processes of cell damage as a result of oxidative stress. Conclusion. Based on the conducted studies, physiological and biochemical biomarkers of the effect of an electric vaporizer with transfluthrin were established. Continuous long-term use of electric insecticide vaporizer can have a harmful effect on the body. The use of these products in ventilated areas according to the consumption rate is safe. To identify the toxic effect of inhalation exposure to transfluthrin, 80 outbred white rats were examined in terms of parameters characterizing the functions of the nervous, respiratory systems, liver, kidneys, and peripheral blood composition.