Phosphate Solubilization Research Articles

Insights from a Genome-Wide Study of Pantoea agglomerans UADEC20: A Promising Strain for Phosphate Solubilization and Exopolysaccharides Production

The genome sequence of Pantoea agglomerans UADEC20 is presented, which is a strain isolated from agricultural fields in northeast Mexico. The genome was assembled into 13 scaffolds, constituting a total chromosome size of 4.2 Mbp, with two of the scaffolds representing closed plasmids. The strain exhibits activity in phosphate solubilization and exopolysaccharide (EPS) production and secretion; therefore, we explored its biotechnological potential via its genome sequencing and annotation. Genomic analyses showed that a total of 57 and 58 coding sequences (CDSs) related to phosphate solubilization and EPS production were identified within its genome, in addition to a reduced number of CDSs related to drug resistance and phages. The comprehensive set of genes supporting phosphate solubilization, EPS synthesis, and secretion, along with its low virulence and antibiotic resistance levels, justify further research for its potential biotechnological application and possible use as a plant growth-promoting agent in the field. These findings suggest a unique genetic background in the P. agglomerans UADEC20 strain.

Characterization, Bio-Prospection, and Comparative Metagenomics of Bacterial Communities Revealing the Predictive Functionalities in Wild and Cultured Samples of Industrially Important Red Seaweed Gracilaria dura.

The present study explores the microbial community associated with the industrially important red seaweed Gracilaria dura to determine the diversity and biotechnological potential through culture and metagenomics approaches. In the first part of the investigation, we isolated and characterized 75 bacterial morphotypes, with varied colony characteristics and metabolic diversity from the wild seaweed. Phylogenetic analysis identified isolates in Proteobacteria, Firmicutes, and Actinobacteria, with Bacillus sp. being prevalent. B. licheniformis and Streptomyces sp. were notable in producing important enzymes like L-asparaginase, and polysaccharide lyases. Antimicrobial activity was significant in 21% of isolates, effective against seaweed pathogens such as Vibrio and Xanthomonas. Rhodococcus pyridinivorans showed strong pyridine degradation, suggesting bioremediation potential. Several isolates exhibited phosphate solubilization and nitrate indicating the roles of bacteria as algal growth promoters and biocontrol agents. Subsequent metagenome analysis of wild and cultured samples provides insights into bacterial communities associated with G. dura, revealing their distribution and functional roles. Proteobacteria (~ 95%) dominated the communities, further bacterial groups involved in algal growth, carpospore liberation, stress resistance, biogeochemical cycles, and biomedical applications were identified. A notable difference in bacteriomes was observed between the samples, with 25% remaining stable. The samples are cultured in the lab to generate seedlings for farming and serve as germplasm storage during the monsoon season. Microbiome surveys are crucial for understanding the association of pathogens and the overall health of the seedlings, supporting successful seaweed farming. Our findings provide valuable insights into G. dura-associated microbial communities and their role in algal growth, which has aquacultural implications.

Fungal Antagonism and Plant Growth Promoting Efficacy of Aspergillus oryzaeAVNF4 isolated from the Rhizome of Curcuma longa on Lycopersicum esculantum L. (tomato)

The present study aimed to develop liquid bioformulation of Aspergillus oryzae AVNF4, a Curcuma longa rhizome endophytic fungus with antagonistic activity against Fusarium oxysporum causing tomato wilt and plant growth promotion of Lycopersicum esculantum. Fungal antagonism, Indole 3- acetic acid production, ammonia and inorganic phosphate solubilisation and GC-MS analysis of antifungal metabolites of A. oryzae AVNF4 were analysed. Liquid bio formulation of A. oryzae AVNF4 (LBF) with enhanced production of IAA, ammonia and inorganic phosphate solubilisation has developed by optimising the culture medium. Seed bacterisation of tomato seeds with LBF for 48h followed by foliar application from the second week onwards showed a significant increase in germination percentage (Gp) and germination index (GI) along with biomass, plant height, shoot length, root length, root/shoot ratio, number of leaves, and soluble protein and reduced sugars in LBF treated plants compared to untreated control. Presence of 1,3-Dioxolane, 2-(1-propenyl)-, L-Prolinamide, 5-oxo-L-prolyl-L-phenylalanyl-4-hydroxy-, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-, dl-Mevalonic acid lactone, Hydro cinnamic acid, Oleic acid, 9-Octadecenoic acid (Z)-, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)-, 1-Allylazetidine, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, 5-Pyrrolidino-2-pyrrolidone, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methyl propyl)-, 9-Octadecenoic acid (Z)-, hexyl ester, Dehydromevalonic lactone, Acetaldehyde, (3,3-dimethyl cyclohexylidene)-, (Z)- in ethyl acetate extract of AVNF4 reported to be the pivotal compounds responsible for fungal antibiosis and to promote growth in tomato seedlings. The field performance of LBF of AVNF4 indicated that A. oryzae AVNF4 is a promising plant growth-promoting endophyte with fungal antagonism against Fusarium wilt.

Trends in the Application of Phosphate-Solubilizing Microbes as Biofertilizers: Implications for Soil Improvement

The application of phosphate-solubilizing microbes (PSMs) as biofertilizers in agricultural systems has not satisfactorily solved the problem of reducing our reliance on chemical phosphorus (P) fertilizers. Ongoing efforts are continually trying to translate promising laboratory results to successful deployment under field conditions, which are typically met with failure. In this review, we summarize the state-of-the-art research on PSMs and their role in the terrestrial P cycle, including previously overlooked molecular and cellular mechanisms underpinning phosphate solubilization. PSMs capable of transforming either organic or complexed inorganic P compounds are discussed. By providing environmentally secure and environmentally friendly ways to increase the accessibility of phosphate, these bacteria effectively transform insoluble phosphate molecules into forms that plants can utilize, encouraging crop growth and increasing nutrient usage effectiveness. The use of PSMs in agriculture sustainably improves crop productivity and has enormous potential for tackling issues with global food security, reducing environmental damage, and promoting sustainable and resilient agricultural systems. Furthermore, due to resource shortages, the changing global climate and need to reduce environmental risks associated with the overuse of chemical phosphate fertilizer, PSMs have the potential to be sustainable biofertilizer alternatives in the agricultural sector. Phosphate-solubilizing microorganisms constitute a cutting-edge field in agriculture and environmental science. In addition, this paper elaborates on the groups and diversity of microbes hitherto identified in phosphate solubilization. Also, factors that had hitherto hindered the reproducibility of lab results in field settings are succinctly highlighted. Furthermore, this paper outlines some biofertilizer formulations and current techniques of inoculation according to the test crop/strain. Finally, laboratory, greenhouse, and field results are presented to acquaint us with the current status of the use of PSM-based biofertilizers.

Seed banking impacts native Acacia ulicifolia seed microbiome composition and function

BackgroundSeed banks are a vital resource for preserving plant species diversity globally. However, seedling establishment and survival rates from banked seeds can be poor. Despite a growing appreciation for the role of seed-associated microbiota in supporting seed quality and plant health, our understanding of the effects of conventional seed banking processes on seed microbiomes remains limited. In this study we investigated the composition and functional potential of seed-associated bacterial epiphytes associated with stored and freshly collected seeds of a native plant, Acacia ulicifolia, using both 16S rRNA gene sequencing and culture-based approaches.ResultsSeeds obtained from seed banking facilities were found to host significantly less diverse bacterial populations, with substantial reductions in both low-abundance taxa and in community members commonly identified in freshly collected A. ulicifolia seeds. Bacteria with key plant growth promoting traits including IAA production, ACC deaminase activity, phosphate solubilisation, siderophore activity, and nitrogen fixation were identified in seed epiphytic communities, but these beneficial traits were less prevalent in stored seed compared to fresh seeds.ConclusionOverall, these results suggest that epiphytic seed microbiomes may undergo significant changes during the storage process, selecting for bacteria tolerant to storage conditions, and potentially reducing the population of plant-growth promoting bacteria on seeds.

Diversity and Plant Growth-Promoting Activities of Culturable Seed Endophytes in Abies pindrow (Royle ex D. Don) Royle: Their Role in Seed Germination and Seedling Growth.

Abies pindrow, a vital conifer in the Kashmir Himalayan forests, faces threats from low regeneration rates, deforestation, grazing, and climate change, highlighting the urgency for restoration efforts. In this context, we investigated the diversity of potential culturable seed endophytes in A. pindrow, assessed their plant growth-promoting (PGP) activities, and their impact on seed germination and seedling growth. We cultured 729 microbial isolates that were resolved into 30 bacterial and 18 fungal species across various phyla. All 48 isolates exhibited various PGP activities. Specifically, all the cultured isolates showed IAA activity with concentrations ranging from 2.07 to 8.453μg/ml, while ammonia production ranged from 0.936 to 3.436mM/ml. Only 18 isolates, predominantly fungi, tested positive for phosphate solubilisation. Additionally, 20 isolates exhibited the ability to inhibit the growth of Fusarium oxysporum f.sp. pini. We selected four bacterial and six fungal isolates, which showed positive results for all PGP activities, to evaluate their effects on seed germination and seedling growth. Notably, seed germination rates increased by 750.9% under bacterial and consortium treatments and by 550.45% under fungal treatment. The consortium treatment also led to a 96% increase in needle count, while bacterial treatment enhanced stem length by 55.4%. Furthermore, shoot biomass also showed a significant increase with both bacterial and fungal treatments, underscoring the potential of harnessing seed endophytes to boost A. pindrow seedling health and resilience. This study underscores the crucial role of seed endophytic diversity in enhancing seed germination, seedling growth, and forest restoration efforts.

Assessment of the Effects of Temperature and Time on the Bacteriological Quality and Heavy Metal of Digestate as a Safe Biofertiliser

The chemical composition and microbial diversity influence the safety, quality and utility of digestate. This study investigated the minimum temperature and time for conversion of digestate to a safe biofertilizer. Digestate, a byproduct of anaerobic digestion was treated at various temperatures, 28 ± 2, 55, 60, 70 and 80 °C at varying durations, 0, 20, 30, 40 and 60 minutes. The potential for rhizobacteria was examined and essentially, at 70 °C and 40 min bacterial counts were 8.05 ± 0.71 × 102 cfu/ml and less than the 1000 cfu/ml permissible limits. At this temperature and time, there were no recorded counts of Salmonella or Escherichiae. Identified species were Pseudomonas sp, Proteus spp., Enterobacter sp., Escherichia coli, Citrobacter sp., Klebsiella sp, Enterococcus sp., Streptococcus sp., Bacillus sp., Yersinia sp., Salmonella sp and Shigella sp. Physicochemical result suggests that digestate processed at 70 °C and 40 mins possess pH, total nitrogen, nitrate and total phosphorus within defined standards while heavy metal concentration was below permissible limits. The rhizobacterial screening showed that Pseudomonas sp., Proteus sp., Bacillus sp. and Streptococcus sp. tested positive for phosphate solubilization, nitrogen fixation, ammonia production and induction of indole acetic acid. Overall, digestate may be successfully deployed as a safe biofertilizer when treated at suitable temperature and time for enhance plant growth.

Oxidized alkaline biochar and phosphate solubilizing bacteria mixture enhances direct seeded maize yield in an acid soil

Oxidized alkaline biochar and phosphate solubilizing bacteria mixture enhances direct seeded maize yield in an acid soil

Optimizing the solubility of low-grade rock phosphate to enable its use as a fertilizer in agriculture

Optimizing the solubility of low-grade rock phosphate to enable its use as a fertilizer in agriculture

Evaluation of spatial variation and nutritional status of environmental quality in the surface seawater from fishing harbours of Hainan island

To investigate the nutrient distribution characteristics and the eutrophication status of the main fishing ports in Hainan Province, this study analyzed the 2021 seawater quality indicators, including biochemical oxygen demand (BOD5), chemical oxygen demand (COD), chlorophyll a (Chl a), ammonia nitrogen (NH4-N), nitrite nitrogen (NO2-N), nitrate nitrogen (NO3-N), and soluble active phosphate (DIP). The single-factor contaminant index method was employed to evaluate the water quality, revealing that the levels of COD, BOD5, and DIP surpass the national seawater quality standards for Type IV. The eutrophication index (EI) of the different fishing ports ranges from 2.38 to 56.29, indicating a high level of eutrophication.

Phosphate solubilizing microorganisms from hypersaline environment: a review

Phosphate solubilizing microorganisms from hypersaline environment: a review

Designing of Phosphate Rich Compost Inoculated with Environmental Strain Pseudomonas aeruginosa AAC1 As A Phosphate Solubilizer

Designing of Phosphate Rich Compost Inoculated with Environmental Strain Pseudomonas aeruginosa AAC1 As A Phosphate Solubilizer

Uji Potensi Isolat Rhizobakteri Menekan Pertumbuhan Jamur Antraknosa Cabai Merah (Capsicum annum L.) secara In Vitro

The main problem in red chili production is the attack of pests and diseases, particularly anthracnose caused by the fungus Colletotrichum spp., which can reduce yields by up to 60% if not controlled. Control methods typically involve the use of fungicides; however, excessive use can lead to pathogen resistance and negative environmental impacts. Therefore, there is a need for more environmentally friendly control alternatives, such as the utilization of biocontrol agents like rhizobacteria. This study aims to evaluate the inhibitory capacity and potential of rhizobacteria in suppressing the growth of Colletotrichum sp. fungi. In the first phase, 20 rhizobacterial isolates were tested, and 5 isolates were selected for further testing in the second screening phase. The research employed a non-factorial Completely Randomized Design, consisting of 6 rhizobacterial treatments with 5 replications, resulting in a total of 30 experimental units. The best inhibitory capacity was observed in rhizobacterial isolate RB 5 (2.44%) and RB 2 (2.41%). All rhizobacterial isolates exhibited variations in colony characteristics, color, and staining results. Additionally, all tested isolates showed potential for phosphate (P) solubilization, indicated by the formation of clear zones around the rhizobacterial suspensions. The highest catalase enzyme production was observed in isolates RB 3 and RB 5. While the rhizobacterial isolate RB 3 demonstrated pathogenicity, as evidenced by the softening of potato tissue after being scratched with the isolate, it still maintained a good inhibitory effect against the growth of Colletotrichum sp.

Evaluation of Soil Beneficial Bacteria as Plant Growth Enhancer and Biocontrol Agents

The use of soil beneficial bacteria, particularly plant growth-promoting rhizobacteria (PGPR), provides an eco-friendly alternative to synthetic agrochemicals in sustainable agriculture. This study aimed to isolate and characterize soil bacterial isolates from the rhizosphere of cabbage cultivation areas in Cameron Highlands, Malaysia, and evaluate their potential as PGPR and biocontrol agents. A total of 42 bacterial isolates were obtained, of which 15 were selected for detailed screening. Morphological and molecular characterization using 16S rDNA sequencing identified species from the genera Bacillus, Pseudomonas, and Serratia. These isolates exhibited key plant growth-promoting traits, including nitrogen fixation, phosphate solubilization, and potassium solubilization. Notably, isolates S9 and S30 demonstrated both plant growth-promoting and antibacterial activities against Xanthomonas campestris, a major pathogen of cabbage. Meanwhile, isolates S16 and S36, although lacking antibacterial activity, showed strong growth-promoting capabilities. These findings highlight the potential of native soil beneficial bacteria as biofertilizers and biocontrol agents, contributing to sustainable agricultural practices by reducing reliance on chemical fertilizers and pesticides. Future research should focus on field trials to validate their efficacy under diverse environmental conditions.

Effect of varying levels of phosphorus and bio-fertilizers on growth and yield of chickpea under sub-tropical condition

A field experiment was conducted at the Crop Research Centre of School of Agriculture, ITM University, Gwalior, Madhya Pradesh during rabi 2021–22 and 2022–23 to study the influence of phosphorus and bio-fertilizers on growth and yield of chickpea under sub-tropical condition of Madhya Pradesh. The experiment was laid out in randomized complete block design (factorial experiment) by taking four levels of phosphorus, viz. 0 kg P2 O5 /ha, 30 kg P2 O5 /ha, 40 kg P2 O5 /ha, 50 kg P2 O5 /ha and bio-fertilizers (No inoculation, PSB (B. thuringiensis) @ 20 g/kg of seed, Rhizobium (Mesorhizobium) @ 20 g/kg of seed PSB (B. thuringiensis) + Rhizobium (Mesorhizobium) which were replicated thrice. The chickpea variety “JG-16” was uniformly fertilized by 20 kg N/ ha through Urea. However, phosphorus and bio-fertilizers were applied as per the requirement of the treatments. The crop was managed as per the recommended package and practices. The experimental study revealed that among the phosphorus levels, the application of 50 kg P/ha recorded significantly highest plant height, number of nodules, shoot dry matter accumulation, number of primary and secondary branches. Further, it also produced highest seed, stover, biological yield and harvest index. Similar, result showed in N, P, K uptake. However, among the bio-fertilizers, application of Phosphate solubilizing bacteria (B. thuringiensis) + Rhizobium Mesorhizobium resulted in highest plant height, number of nodules, shoot dry matter accumulation, number of primary and secondary branches, seed and stover yield, biological yield and harvest index of chickpea. Application of 50 kg ha-1 phosphorus and combined treatment of Phosphate solubilizing bacteria (B. thuringiensis) + Rhizobium (Mesorhizobium) resulted in highest gross return, net return and Benefit: cost ratio.

Effect of varying levels of phosphorus and bio-fertilizers on growth and yield of chickpea under sub-tropical condition

A field experiment was conducted at the Crop Research Centre of School of Agriculture, ITM University, Gwalior, Madhya Pradesh during rabi 2021–22 and 2022–23 to study the influence of phosphorus and bio-fertilizers on growth and yield of chickpea under sub-tropical condition of Madhya Pradesh. The experiment was laid out in randomized complete block design (factorial experiment) by taking four levels of phosphorus, viz. 0 kg P2 O5 /ha, 30 kg P2 O5 /ha, 40 kg P2 O5 /ha, 50 kg P2 O5 /ha and bio-fertilizers (No inoculation, PSB (B. thuringiensis) @ 20 g/kg of seed, Rhizobium (Mesorhizobium) @ 20 g/kg of seed PSB (B. thuringiensis) + Rhizobium (Mesorhizobium) which were replicated thrice. The chickpea variety “JG-16” was uniformly fertilized by 20 kg N/ ha through Urea. However, phosphorus and bio-fertilizers were applied as per the requirement of the treatments. The crop was managed as per the recommended package and practices. The experimental study revealed that among the phosphorus levels, the application of 50 kg P/ha recorded significantly highest plant height, number of nodules, shoot dry matter accumulation, number of primary and secondary branches. Further, it also produced highest seed, stover, biological yield and harvest index. Similar, result showed in N, P, K uptake. However, among the bio-fertilizers, application of Phosphate solubilizing bacteria (B. thuringiensis) + Rhizobium Mesorhizobium resulted in highest plant height, number of nodules, shoot dry matter accumulation, number of primary and secondary branches, seed and stover yield, biological yield and harvest index of chickpea. Application of 50 kg ha-1 phosphorus and combined treatment of Phosphate solubilizing bacteria (B. thuringiensis) + Rhizobium (Mesorhizobium) resulted in highest gross return, net return and Benefit: cost ratio.

Characterization of endophytic bacteria in Vietnamese rice seeds

Rice is a staple food and is commonly used in daily life not only in Vietnam but worldwide. However, pests, diseases, and nutrient deficiencies are threatening Vietnam's rice production by causing significant damage. The endophytic bacteria (EB) which are isolated from plants, may help improve certain quality traits of rice seeds assist plants in coping with abiotic and biotic stresses from the environment. Therefore, this study aims to investigate some promising characteristics of EBs in rice seeds, including gelatinase production, starch hydrolysis, phosphate solubilizing, cellulase, and IAA synthesis. Rice seeds from various rice varieties were used to isolate the endophytic bacteria. The bacteria were grown in Petri dishes or glass test tubes in some selective media in a controlled environment to screen for investigated traits. A total of five EBs were isolated, and MALDI-TOF mass spectrometry with a log score of MALDI Biotypes greater than 2.0 was employed for bacterial identification. Interestingly, the results revealed that the bacteria in rice grains have a high ability to synthesize cellulase, hydrolyze starch and gelatin, and produce auxin. The highest cellulase activity was associated with Staphylococcus caprae while Micrococcus luteus exhibited maximum IAA hormon and gelatinase enzyme production. Starch hydrolysis was highest in Bacillus. However, these bacteria showed low phosphate solubilization ability. The promising bacteria identified in this study include Bacillus cereus, M. luteus, and Bacillus atrophaeus. The promising results from our study can be utilized for further in vivo studies in rice plants to develop biocontrol reagents and biofertilizers for agricultural applications.

Sinomonas gamaensis NEAU-HV1 remodels the IAA14-ARF7/19 interaction to promote plant growth.

Sinomonas species typically reside in soils or the rhizosphere and can promote plant growth. Sinomonas enrichment in rhizospheric soils is positively correlated with increases in plant biomass. However, the growth promotion mechanisms regulated by Sinomonas remain unclear. By using soil systems, we studied the growth-promoting effects of Sinomonas gamaensis NEAU-HV1 on various plants. Through a combination of phenotypic analyses and microscopic observations, the effects of NEAU-HV1 on root development were evaluated. We subsequently conducted molecular and genetic experiments to reveal the mechanism promoting lateral root (LR) development. We demonstrated that NEAU-HV1 significantly promoted the growth of lettuce, wheat, maize, peanut and Arabidopsis. This effect was associated with multiple beneficial traits, including phosphate solubilization, indole-3-acetic acid and 1-aminocyclopropane-1-carboxylic acid deaminase production and survival ability in the rhizosphere and within the inner tissue of roots. In addition, NEAU-HV1 could secrete metabolites to promote LR development by affecting auxin transport and signaling. Importantly, we found that the influence of auxin signaling may be attributed to the remodeling interaction between SOLITARY-ROOT (SLR)/IAA14 and ARF7/19, occurring independently of the auxin receptor TIR1/AFB2. Our results indicate that NEAU-HV1-induced LR formation is dependent on direct remodeling interactions between transcription factors, providing novel insights into plant-microbe interactions.

Применение эндофитных микроорганизмов для интенсификации ростовых процессов сельскохозяйственных культур

Increasing the yield of wheat, barley, and oats is a pressing issue. It largely depends on soil fertility. Mineral fertilizers, however, may be ineffective and unsustainable. As a result, microorganisms seem to be a promising alternative. The authors isolated endophytic microorganisms with growth-stimulating properties and assessed their effect on the growth rate of wheat, barley, and oats in laboratory conditions. The research involved spring soft wheat of the Sibirsky Alyans variety, spring oats of the Maruchak variety, spring barley of the Nikita variety, and standard bacterial strains (Azospirillum brasilense B-11094, Azotobacter chrococcum B-8739). The isolated bacteria were identified using a Vitex 2 Compact automatic microbiological analyzer. The production potential for indole-3-acetic and gibberellic acids was assessed spectrophotometrically. The nitrogen fixation potential was determined using a Rapid N Cube. The phosphate-solubilizing potential was tested on a calcium phosphate medium. The effect of the most promising strains on the growth rate was assessed in laboratory conditions. Seven isolates of endophytic microorganisms were identified as Pantoea allii Tri, Bacillus subtilis Tri 2, Bacillus subtilis Ave 1, Pantoea allii Ave 2, Bacillus subtilis Hor 1, Bacillus subtilis Hor 2, and Bacillus subtilis Hor 3. The most promising growth promoters ranged as follows. Bacillus subtilis Ave 1 fixed 790 μg/mL nitrogen, solubilized phosphates with index 1.60, and produced 7100 μg/mL indolyl-3-acetic acid and 343 μg/mL gibberellic acid. Bacillus subtilis Hor 1 fixed 760 μg/mL nitrogen, solubilized phosphates with index 1.44, and synthesized 4490 μg/mL indolyl-3-acetic acid and 409 μg/mL gibberellic acid. Bacillus subtilis Ave 1 demonstrated the greatest growth-stimulating activity. Bacillus subtilis Ave 1 could synthesize phytohormones, fix atmospheric nitrogen, and solubilize phosphates, which indicated good agricultural prospects. The strain increased the length of shoots and roots in wheat and barley, as well as boosted germination and shoot length in oats.

Antagonistic Potential of a Phosphate Solubilizing Bacteria (B. cereus PS1.1, B. cereus PS1.2, B. cereus PS1.4) Against the Patogent Fungus Ganoderma sp. Isolated from Basal Stem of Oil Palm (Elaeis guineensis Jacq.) with Rot Disease

Ganoderma sp. is a pathogenic fungus whose attack can cause basal stem rot disease of oil palm (Elaeis guineensis Jacq.). Disease control using phosphate solubilizing bacteria (PSB), namely Bacillus cereus can be an alternative to biological control. The purpose of this study was to determine the antagonistic ability of PSB (B. cereus PS1.1, B. cereus PS1.2, B. cereus PS1.4) in inhibiting the growth of Ganoderma sp. BP1 and changes in hyphal morphology of Ganoderma sp. BP1 after antagonistic testing. The research was conducted from January to May 2023 at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak. Antagonist testing used a completely randomized design (CRD) with the treatments consisted of Ganoderma sp. BP1 (negative control), 1% hexaconazole fungicide (positive control), PSB isolates PS1.1, PS1.2 and PS1.4. The test method used the dual culture on Sabouraud Dextrose Agar (SDA) media with each treatment repeated four times so that 20 experimental units were obtained. The results showed that PSB isolate PS1.4 had strong inhibition with an inhibition zone diameter of 11.01 mm, while isolates PS1.1 and PS1.2 had moderate inhibition with inhibition zone diameters of 9.43 mm and 9.45 mm, respectively, against Ganoderma sp. BP1. Hyphal morphology changes in of Ganoderma sp. BP1 that occurred after the antagonist test consist of lysed hyphae, twisted hyphae, hook-like hyphal tips, curly hyphae, bulbous hyphae, branched hyphae and bent hyphal ends.