Inorganic Arsenic Compounds Research Articles

Ultrasensitive detection of four organic arsenic compounds at the same time using a five-link cardboard-based assay

In order to effectively control the excessive use of organic arsenic reagents in livestock and poultry products, there is an urgent need to develop a method for rapid detection of multiple organic arsenic reagents. In this study, two haptens were designed and derivatized around the structural formula of roxarsone, and a highly-sensitive group-selective mAb 3F2 was prepared, which can simultaneously detect roxarsone, 4-aminophenylarsonic acid, 2-aminophenylarsonic acid and phenylarsonic acid. We further developed a colloidal gold immunochromatographic test strip (ICS) and prepared a five-link card that can simultaneously detect four organic arsenics in chicken and pork samples. Its quantitative detection limits (LOQ) for the four compounds in chicken and pork samples were 0.06 and 0.32 ng/mL, 0.11 and 0.29 ng/mL, 0.34 and 0.99 ng/mL, and 0.88 and 1.5 ng/mL, respectively. This multi-ICS detection provides a powerful tool for the on-site detection and rapid screening of organic arsenic reagents in actual samples.

Asian Multinational Phase II Study of Darinaparsin in Patients with Relapsed or Refractory Peripheral T-Cell Lymphoma

Asian Multinational Phase II Study of Darinaparsin in Patients with Relapsed or Refractory Peripheral T-Cell Lymphoma

Deterministic & stochastic models in obtaining arsenic-Compounds-free produces in hydroponic mediums added with double sieving’s with small planktonic populations in nano-biotechnological application

Here a simple and hi-tech applications is delivered with nano-biotechnological applications. Nano-biotechnology is the branch of biotechnology that deals with making structures that are ranged between 0- 500 nanometres of dimension. Molecular diameter of Arsenic and its compounds usually falls within this range and appropriately porous Phytoplankton cells can accumulate this enzymes inhibition bio-molecules. As we all know that Phytoplankton is the base of several aquatic food webs. Phytoplankton, also known as microalgae, are similar to terrestrial plants in that contain chlorophyll and require sunlight in order to live and grow can be provided to Hydroponic mediums as energy supplements. Mere sieving of arsenic compounds by phytoplankton and providing safety hydroponic foods. From both the biological and the toxicological points of view, arsenic compounds can be classified into three major groups: inorganic arsenic compounds; organic arsenic compounds; and arsine gas and all these can be sieved with plankton populations of small and medium in sizes. Nanotechnology and nano-biotechnology with Plankton population are used in recent days for mankind. Arsenic or its compounds can be termed as nano-particles having environmental hazards to all biological cells, tissue, organs. As per molecular dimensions arsenic and arsenic compounds can be sieved by certain plankton populations. Small algae and medium algae can sieve arsenic or its compounds through their appropriate porous cells and accumulate within and can make the aquatic medium free from arsenic and which can be used for hydroponic culture medium and can produce Arsenic free fruit and vegetables.

Modelling of almost total depleted arsenic- compounds from hydroponic mediums may possible using methodical, computational, plankton Nanobiotechnology

The nanobiotechnology is the branch of technology that deals with making structures that are ranged between 0- 500 nanometres of dimension. Molecular diameter of Arsenic and its compounds usually falls within this range and appropriately porous Phytoplankton cells can accumulate this enzymes inhibition bio-molecules. As we all know that Phytoplankton is the base of several aquatic food webs. Phytoplankton, also known as microalgae, are similar to terrestrial plants in that contain chlorophyll and require sunlight in order to live and grow can be provided to Hydroponic mediums as enery supplements. Mere sieving of arsenic compounds by phytoplankton and providing safety hydroponic foods. From both the biological and the toxicological points of view, arsenic compounds can be classified into three major groups: inorganic arsenic compounds; organic arsenic compounds; and arsine gas and all these can be sieved with plankton populations of small and medium in sizes. Nanotechnology and nanobiotechnology with Plankton population are used in recent days for mankind. Arsenic or its compounds can be termed as nano-particles having environmental hazards to all biological cells, tissue, organs. As per molecular dimensions arsenic and arsenic compounds can be sieved by certain plankton populations. Small algae and medium algae can sieve arsenic or its compounds through their appropriate porous cells and accumulate within and can make the aquatic medium free from arsenic and which can be used for hydroponic culture medium and can produce Arsenic free fruit and vegetables.

Efficient removal of roxarsone and emerging organic contaminants by a solar light-driven in-situ Fenton system

High-risk roxarsone (ROX) can be converted to highly toxic arsenite (As(III)) through biological and abiotic processes, which can cause severe arsenic contamination of water and crops. Traditional oxidation processes can only treat organic arsenic compounds instead of releasing inorganic arsenic (iAs). Herein, an Fe(II)/resorcinol formaldehyde resin (RF) solar light-driven in-situ Fenton system was applied to remove ROX and its released iAs. Under solar light illumination, RF can reduce oxygen to produce H2O2 through a two-electron reduction pathway. Fe(II) simultaneously catalyzed the decomposition of H2O2 to generate ·OH, participating in the oxidation of ROX. The C-As bond of ROX was preferentially attacked by ·OH to form 2-nitrohydroquinone and As(III). Moreover, As(III) was further converted to low-toxicity arsenate (As(V)) through further oxidation with oxygen bubbling. Finally, the released As(III) (1.9%) and As(V) (98.1%) were removed by adsorption onto the surface of ferric hydroxide. As a result, more than 99.9% of ROX was degraded by the Fe(II)/RF in-situ Fenton system during the pre-oxidation process. The total organic carbon (TOC) removal efficiency of ROX and total arsenic removal efficiency of the Fe(II)/RF-O2 in-situ Fenton system reached 75% and more than 97%, respectively. Furthermore, the residual arsenic in this Fenton system was less than 12.5 µg/L, which met the recommended limit for arsenic (100 µg/L) in reclaimed water. In addition to the removal of ROX, the Fe(II)/RF in-situ Fenton system showed excellent general applicability in the removal of emerging organic contaminants (EOCs). The TOC removal rate of EOCs was approximately 50%–64%. Thus, the Fe(II)/RF-O2 solar light-driven in-situ Fenton system provides a novel strategy for the high-efficiency removal of organoarsenic compounds and EOCs.

On the effect of salvarsan on blood clotting. Trost (Arch. f. Derm. u. Syph., 1922, Bd. 139)

In order to find out the effect of organic arsenic compounds in the form of salvarsan and its derivatives on blood clotting the author set a series of in vitro and in vivo experiments by the methods of Brkеrʹa and Schultz'a. These experiments convinced the author that organic arsenic compounds such as salvarsan already in minimal amounts cause in vitro clotting retardation.

A novel transformation pathway of p-arsanilic acid in water by colloid ferric hydroxide under UVA light.

Iron species that occur in natural surface water could affect the photochemical behavior of pollutants. Complexation between iron species and polycarboxylate or heavy metals has been widely reported, where the ligands could be oxidized via ligand-to-metal charge transfer (LMCT) by light inducement. Such complexation and photochemical reactions might also occur for low valance metal-containing organic compounds, which is worthy of investigation. This work studied the phototransformation of p-arsanilic acid (ASA), an organic arsenic compound that is widely used as a feed additive in the poultry industry, by colloidal ferric hydroxide (CFH) using black light lamps (λ = 365 nm) as the light source. The results revealed the contribution to ASA transformation at circumneutral conditions by CFH through an LMCT process, which is the same as that for As(III). The complexation between ASA and CFH was investigated using UV-vis spectroscopy. The estimated equilibrium constant for the CFH-ASA complex was log Kf271 = 4.22. The analysis of the photoproducts found the generation of both inorganic and organic arsenic. Our findings confirmed the similarities in the photochemical mechanisms of ASA and As(III) in the presence of CFH. The results help in further understanding the fate of organoarsenicals in the surface water environment.

210 cases of relapsing fever baked by neosal-varsan

The issue of treating relapsing fever with organic arsenic compounds is 15-17 years old.

Arsenic trioxide induces expression of BCL-2 expression via NF-κB and p38 MAPK signaling pathways in BEAS-2B cells during apoptosis

Inorganic arsenic compounds are environmental toxicants that are widely distributed in air, water, and food. B-cell lymphoma 2 (BCL-2) is an oncogene having anti-apoptotic function. In this study, we clarify that BCL-2, as a pro-apoptotic factor, participates in As2O3-induced apoptosis in BEAS-2B cells. Specifically, As2O3 stimulated the expression of BCL-2 mRNA and protein in a dose-dependent manner which was highly accumulated in the nucleus of BEAS-2B cell together with chromatin condensation and DNA fragmentation during apoptosis. Mechanistically, the process described above is mediated through the NF-κB and p38 MAPK signaling pathways, which can be abated by corresponding inhibitors, such as BAY11–7082 and SB203580, respectively. Additionally, BAY11–7082, actinomycin D, and cycloheximide have inhibitory effects on As2O3-induced expression of BCL-2 mRNA and protein, and restore the cell viability of BEAS-2B cells. Suppression of BCL-2 protein activation by ABT-199 also restored viability of BEAS-2B cell in As2O3-induced apoptosis. Furthermore, As2O3 increased the level of BCL-2 phosphorylation. These results suggest that in BEAS-2B cells, As2O3-induced apoptosis is mainly dominated by BCL-2 upregulation, nuclear localization and phosphorylation. The study presented here provides a novel insight into the molecular mechanism of BCL-2-induced apoptosis.

Arsenic speciation in fish from Baltic Sea close to chemical munitions dumpsites

Chemical weapons that were dumped in seas and oceans after World War II, including the Baltic Sea, are sources of pollution of marine areas. Sunken containers can corrode, unseal, and numerous compounds pass into the environment, including toxic forms of arsenic, which are then taken up by marine animals. This study aims to quantify concentration of total arsenic, inorganic arsenic (III + V), and organic compounds arsenobetaine, monomethylarsonic acid, dimethylarsinic acid in the muscle tissues of cod, herring, sprat, and flounder and the associated risk to consumer health. Sprat muscle (0.636 mg kg−1) had the highest content of total arsenic, significantly less was noted in the muscles of herring (0.460 mg kg−1) and flounder (0.588 mg kg−1), and the least was in cod (0.390 mg kg−1). Toxic inorganic arsenic compounds were present in the fish tested at levels below 0.02 mgkg−1 and constituted from 3.45 to 5.75% of total arsenic. Arsenobetaine dominated among organic forms, and concentrations of it, like total arsenic, varied depending on the fish species. Consumer health risk was determined with the estimated daily intake, the target hazard quotient, and the carcinogenic risk. Estimated daily intake values for inorganic arsenic in herring, cod, sprat, and flounder were below the reference dose at 0.51 × 10−5 mg kg−1 b. w. day. The target hazard quotient factor of 0.0017 indicated there was no threat. Carcinogenic risk values were within the permissible range of 10−6 to 10−4. Current data indicate that inorganic arsenic compounds pose no risk to the health of consumers of Baltic fishes.

Báo Cáo Một Trường Hợp Ngộ Độc Asen Cấp Tính Được Trị Thành Công Tại Bệnh Viện Nhi Trung Ương

Arsenic is a poisoning metallic element, in which trivalent inorganic arsenic compounds are considered unique. Children can be exposed from arsenic containing water, food and folk remedies with unclear ingredients [1]. The main toxic mechanism of As3+ is inhibition of the pyruvate dehydrogenase (PDH) complex, which leads to decrease in acetyl-CoA production, decrease in cellular respiration and free oxygen radical (0-) and hydrogen peroxide (H202) are born, which make cytotoxic. Arsenic poisoning causes damage to multiple organs: keratosis of the skin, nail and squamous cell cancer, neuropathy, repolarization of the myocardium, liver damage and subsequent sequelaes [1], [2]. Arsenic poisoning has no specific symptoms, so it is easy to overlook, especially in children [2]. The epidemiological exploitation and history of poisoning drugs are very important to avoid missing arsenic poisoning. We report a case of successfully treated arsenic poisoning at the Vietnam National Children’s Hospital.

Roxarsone Promotes Glycolysis and Angiogenesis by Inducing Hypoxia-Inducible Factor-1α In Vitro and In Vivo.

Roxarsone (Rox) is an organic arsenic compound used as a feed additive to promote animal growth. The release of Rox into the environment poses risks to human health. Rox demonstrated tumor-promoting and proangiogenic effects in xenograft models. Increasing studies revealed the tight relationship among angiogenesis, carcinogenesis, tumorigenesis, and glycolysis. Glycolysis, via hypoxia-inducible factor-1α (HIF-1α), controls vascular endothelial cell (VEC) growth. To date, there has been no literature report on the effect of Rox on HIF-1α-dependent glycolysis. Herein, we report that Rox promoted glycolysis in rat VECs, as shown by the increased adenosine triphosphate production, the lactic acid release, the activity and content of aldolase (ALD), and the expression levels of ALD A and glucose transporter 1 (GLUT1). Rox also increased the cellular levels of HIF-1α. Treatment with the HIF-1α inhibitor YC-1 reversed Rox-increased ALD A and GLUT1 levels and attenuated Rox-induced VEC viability, suggesting that Rox-induced HIF-1α contributes to the glycolytic and angiogenic effects of Rox. Rox also promoted tumor growth and angiogenesis and increased the levels of ALD A, GLUT1, and HIF-1α in the tumor tissue of a mouse xenograft model, whereas these effects were abolished using YC-1. Our findings indicated that Rox induces HIF-1α in VECs to promote glycolysis and angiogenesis thus enhancing the tumor growth.

Absorption and speciation of arsenic by microalgae under arsenic-copper Co-exposure

Combined pollutions of arsenic (As) and copper (Cu) are common in water bodies near mines, non-ferrous metal smelting and power plants. This study investigated the effect of Cu(II) on the absorption and speciation of As(V) by microalgae. We compared the absorption and speciation of arsenic by microalgae (mainly Cyanophyta and Chlorophyta) when exposed to single As(V) with that exposed to As-Cu co-exposure in laboratory. The results showed that in the case of single As(V) exposure, the inhibitory effect of As(V) on microalgae was primarily affected by the exposure time, instead of the concentration of As(V) in the water solution. Compared with single As(V) exposure, the presence of Cu(II) under As-Cu co-exposure promoted the absorption and accumulation of As(V) by algae. The combination effect of As and Cu on algae was antagonistic instead of synergistic within the tolerance range of algae to them. In the presence of Cu(II), more monomethylarsonous acid (MMA) and dimethylarsinous acid (DMA), which are volatile organic arsenic compounds, were produced in algae compared with the control. The finding that Cu(II) can mediate the absorption and speciation processes of arsenic in algae has significance in possible bioremediation of arsenic pollution in aquatic environment.

Nanocomposites of graphene and zirconia for adsorption of organic-arsenic drugs: Performances comparison and analysis of adsorption behavior

3-Nitro-4-hydroxy-phenylarsonic acid (3-NHPAA), an organic-arsenic compound, as one of widely used antibacterial veterinary drug, has greatly attracted the attention due to its potential threats on ecological environment. A series of the nanocomposites of zirconia nanoparticles with crystal phases (pure monoclinic, pure tetragonal and mixed phase (monoclinic + tetragonal)) anchored on reduced graphene oxide were produced through managing the concentration of triethanolamine solution and the reaction time. The effects of the crystal phases of the zirconia in the structure of the nanocomposites were played a key role in the adsorption performances of the 3-NHPAA. Experiment data identified the nanocomposites with monoclinic phase of zirconia excelled at the adsorption of the 3-NHPAA with a higher adsorption capacity up to 207.2 mg g−1. The uptake of the 3-NHPAA by the three nanocomposites was implemented within 60 min and highly pH-dependent which illustrated electrostatic attraction between them as a main mechanism during the adsorption process. A wider pH range (3.8–8.8) for the uptake of the 3-NHPAA by the nanocomposites with the monoclinic phase of zirconia was obtained compared with the nanocomposites containing tetragonal phase (3.8–5.9) or the mixed phase (3.8–7.1) of zirconia. The adsorption of the 3-NHPAA was well described by the pseudo-second order kinetic and Langmuir equations. The thermodynamic parameters suggested that the adsorption of the 3-NHPAA over the three nanocomposites was endothermic and spontaneous in nature. In summary, the nanocomposites of reduced graphene oxide and monoclinic phase of zirconia nanoparticles as an adsorbent were better to the adsorption of the 3-NHPAA.

Total arsenic and water-soluble arsenic species in foods of the first German total diet study (BfR MEAL Study)

Arsenic can occur in foods as inorganic and organic forms. Inorganic arsenic is more toxic than most water-soluble organic arsenic compounds such as arsenobetaine, which is presumed to be harmless for humans. Within the first German total diet study, total arsenic, inorganic arsenic, arsenobetaine, dimethylarsinic acid and monomethylarsonic acid were analyzed in various foods. Highest levels of total arsenic were found in fish, fish products and seafood (mean: 1.43 mg kg−1; n = 39; min–max: 0.01–6.15 mg kg−1), with arsenobetaine confirmed as the predominant arsenic species (1.233 mg kg−1; n = 39; min–max: 0.01–6.23 mg kg−1). In contrast, inorganic arsenic was determined as prevalent arsenic species in terrestrial foods (0.02 mg kg−1; n = 38; min–max: 0–0.11 mg kg−1). However, the toxicity of arsenic species varies and measurements are necessary to gain information about the composition and changes of arsenic species in foods due to household processing of foods.

Urinarni metaboliti kao indikatori izloženosti ljudi hemijskim karcinogenima

Population exposure to environmental chemical carcinogens is a growing public health problem. Carcinogenic chemicals may be classified into two groups: genotoxic and non-genotoxic. A genotoxic chemical has a potential to induce the development of cancer, either in direct interaction with DNA or with cell structures, which are responsible for the maintenance of genome integrity. A non-genotoxic chemical has a potential to induce cancer indirectly by entering the processes of cancer etiopathogenesis. Previous research studies indicate that inorganic arsenic compounds may be associated with various malign diseases (lung cancer, urinary bladder cancer, skin, kidney, liver and prostate cancer). Inorganic arsenic is mainly present in meat, dairy products and grains, while organic arsenic (arsenobetaine) is present in seafood, fruit and vegetables. Benzene metabolites are associated with different types of leukemias and lymphomas, benzidine with bladder cancer, nickel with lung cancer, chromium compounds with lung cancer, nose and nasal sinus cancer. The greatest occupational exposure to benzene is in industry (leather, electronic device, shoes, sports equipment), while people may come into contact with benzidine through consumer goods (leather products, clothes, toys). The highest concentrations of nickel were measured in the beans, walnuts and grains. Cadmium and cadmium compounds cause lung cancer, and influence the occurrence of renal and prostate cancer. The risk of hepatocellular carcinoma is significantly increased in respondents with high concentrations of urinary metabolites of aflatoxin (aflatoxin N7-gvanine adducts). Lindane isomers are present in dairy products, meat, fish, poultry, garden fruit, oils and lipids, leaf and root vegetables and sugar, and they cause non-Hodgkin lymphoma. There is a positive correlation between the consumption of Aristolochia plants and the occurrence of urothelial carcinoma. There are no screening examinations for the identification of persons who are at great risk of developing malign disease in the next 10 or 20 years. As for the prevention of malign diseases, it is necessary to put an accent on finding the adequate methods for determining the concentrations of urinary metabolites for toxic chemical carcinogens and define their risk values.

MOF-on-MOF Membrane with Cascading Functionality for Capturing Dichromate Ions and p-Arsanilic Acid Turn-On Sensing.

It is very significant that functional porous metal-organic frameworks are used to manufacture hierarchical components to achieve cascading functions that cannot be achieved by a single-layer metal-organic framework (MOF). Here, we report two cases of novel MOFs constructed by the same ligand, Cu(I)-tpt and Cu(II)-tpt (Htpt = 5-[4(1H-1,2,4-triazol-1-yl)]phenyl-2H-tetrazole), and prepared a Cu(II)-tpt-on-Cu(I)-tpt membrane by a layer-by-layer approach ignoring the lattice mismatch problem. The first Cu(I)-tpt layer is grown on an oriented Cu2O nanostructured array by a "one-pot" approach. The aligned second Cu(II)-tpt layer can be deposited using liquid-phase epitaxy. Notably, the prepared Cu(II)-tpt-on-Cu(I)-tpt membrane combines adsorption and fluorescence sensing, which exhibited significant adsorption for Cr2O72- (203.25 mg g-1) as typical highly poisonous ions with a fluorescence quenching response. Hence, based on the oxidation-reduction between Cr2O72- and p-arsanilic acid (p-ASA), the Cu(II)-tpt-on-Cu(I)-tpt membrane's ability to adsorb Cr2O72- could be used to design "on-off-on" mode fluorescence probes to detect p-ASA with high sensitivity (limit of detection (LOD) = 0.0556 μg L-1). p-ASA can be degraded into highly toxic inorganic arsenic compounds in the natural environment and has received widespread attention. Therefore, the integration of adsorption and fluorescence properties makes the Cu(II)-tpt-on-Cu(I)-tpt membrane a feasible multifunctional material for pollution control and detection.

Genentech, Inc. v. Immunex Rhode Island Corp. United States Court of Appeals of the Federal Circuit, 2020 964 F.3d 1109

Genentech, Inc. v. Immunex Rhode Island Corp. United States Court of Appeals of the Federal Circuit, 2020 964 F.3d 1109

Breast Cancer Disease and Arsenic: “Geological Behavior (GBR) as Key “

Geology may appear remote from human health. However, rocks are the fundamental building blocks of the Earth’s surface, full of important minerals and chemical elements. Rock weathering and alteration products not only form our soils to support our crops but provide essential elements to build biology. Some heavy metals are carcinogenic, and some only affect the body’s ability to produce red and white cells. In fact, any heavy metal affects any part of the body due to our geological behavior (GBR). For example, a heavy metal may not cause any side effects by contact with the skin, even when swallowed, and may not cause serious harm, but inhaling the vapor of that heavy, toxic metal can cause severe damage to the lungs. Some heavy and quasi metals are more toxic, and some forms of heavy metal compounds are even more dangerous than other forms. Toxic and hazardous heavy metals like arsenic, lead, mercury, zinc, chromium and iron are found in a variety of personal care products, e.g., lipstick, whitening toothpaste, eyeliner and nail color. We use the Anodic Stripping voltammetry (ASV) method for arsenic detection, utilizing the PDV6000 + analyzer (Manufactured B3 Electronics, Perth Australia) configured with a solid gold electrode for total as analyses. Sample Preparation: Spot urine samples were collected from most of the participants from 1 January 2018 to 30 March 2020 after face to face interviews. Women with missing information on adjustment variable(s) were included in the analysis in a separate category for adjustment. Results: Our studies were focus in describing the mean Urinary Arsenic levels (u As) residents of in the central zones of Shiraz, Fasa, Abadeh, Jahrom and Marvdasht to compare the effect of diet such as consuming canned or frozen foods, Consume rice daily, smoking habits and lifestyle on the incidence of Breast. Conclusion: Cancer with arsenic compounds has been reported in many parts of the world. The disease affects all organs of the body, and every year many victims of cancer suffer from severe symptoms and even death. Arsenic compounds are present in the human body and environment in an inorganic and organic form. Inorganic arsenic includes arsenite and arsenic, can also be presented in the environment in the form of methyl (monthly arsenic acid and dimethyl arsenic acid). Arsenic, especially inorganic arsenic, is well absorbed through the gastrointestinal tract rivers (80% -90%) and will be scattered throughout the body. It is often metabolized by methylation and excreted mainly in the urine. Methylation of inorganic arsenic compounds is said to undergo a detoxification process.

Occurrence, speciation analysis and health risk assessment of arsenic in Chinese mitten crabs (Eriocheir sinensis) collected from China

Arsenic (As) is a major hazardous element in natural environments, and arsenic pollution is becoming an issue of concern worldwide. The more toxic inorganic arsenic, such as trivalent arsenite As(Ⅲ) and pentavalent arsenate As(Ⅴ), which normally can transform to organic arsenic compounds, such as arsenobetaine (AsB), arsenocholine (AsC), monomethyl arsenic acid (MMA), dimethyl arsenic acid (DMA), trimethyl arsenic acid (TMA), arsenosugars and arsenolipids. In this study, a total of 2130 individuals of Chinese mitten crabs were collected from seven locations and mixed to 71 samples. Total arsenic and six major species (AsC, AsB, DMA, MMA, As (Ⅲ), and As (Ⅴ)) were determined by inductively coupled plasma mass spectrometry (ICP-MS) and high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS), respectively. The target hazard quotient (THQ) was utilized to evaluate the human health risk. The total arsenic concentration and totals for the six arsenic forms are 0.25–1.66 mg/kg and 0.05–1.19 mg/kg wet mass, respectively. Except for HH and LH, the six arsenic species concentrations accounted for more than 50.0 % of the total arsenic. The less toxic AsB is the most predominant in crabs, comprising 50.0 %–90.0 % of the sum of six types of arsenic. The more toxic inorganic arsenic only range from 0.01 to 0.21 mg/kg wet mass, much less than the limit content of 0.50 mg/kg inorganic As in crustacea. The THQ values of inorganic arsenic through the consumption of Chinese mitten crabs are estimated, and the values are all less than 1, indicating that intaking Chinese mitten crabs collected from China will not cause an appreciable hazard risk to human health.