Arbuscular mycorrhizal fungi (AMF) is a broad-spectrum non-specific organism known to colonize 85% of land plants hold considerable potential for use as inoculants. In the present perspective much attention is focussed on mass production of AMFinoculum, since this is of paramount importance in improving better uptake of nutrients, offers tolerance against a range of soil stresses, plant production and enhances the chances of plant survival. Given these benefits, utilization of the AMF symbiosis should be an important tool in sustainable agricultural systems. Producing AMF inoculum is a complex procedure involving selection of a host plant, finding the right mix or medium and the inoculums starter. Seven different substrates were tested for the production of AMF inoculum. Red earth in isolation or combination with solirite emerged as a suitable potential medium when compared to solirite for bulk production of pure, mature and infective AMF inoculum.