Innovative Extraction Method Research Articles

Macauba (Acrocomia ssp.) fruits: A comprehensive review of nutritional and phytochemical profiles, health benefits, and sustainable oil production.

Macauba is an underexplored palm with significant potential for food-grade vegetable oil production. Its fruits yield two distinct sources of oil, the pulp and the kernel, each with its unique composition, emerging as a potential vegetable oil source with high competitiveness with well-established conventional oil sources. Besides the oil, macauba fruits are rich in essential nutrients, including proteins, minerals, vitamins, dietary fiber, and phytochemicals, with outstanding health benefits. Macauba processing generates valuable co-products, including the epicarp, pulp and kernel cakes, and endocarp, which have considerable potential for enhancing the macauba production chain. This review explores the nutritional and phytochemical profile of macauba, its health benefits, and the potential for exploiting its co-products. Innovative extraction methods and a comprehensive strategy for producing multiple products from macauba co-products are also highlighted as opportunities to achieve sustainable development goals and a circular economy in macauba fruit processing.

Exploring bioactive compounds in lyophilized grape pomace extracts: comparative analysis of traditional and innovative extraction methods

Exploring bioactive compounds in lyophilized grape pomace extracts: comparative analysis of traditional and innovative extraction methods

Lithium extraction from low-quality brines.

In the quest for environmental sustainability, the rising demand for electric vehicles and renewable energy technologies has substantially increased the need for efficient lithium extraction methods. Traditional lithium production, relying on geographically concentrated hard-rock ores and salar brines, is associated with considerable energy consumption, greenhouse gas emissions, groundwater depletion and land disturbance, thereby posing notable environmental and supply chain challenges. On the other hand, low-quality brines-such as those found in sedimentary waters, geothermal fluids, oilfield-produced waters, seawater and some salar brines and salt lakes-hold large potential owing to their extensive reserves and widespread geographical distribution. However, extracting lithium from these sources presents technical challenges owing to low lithium concentrations and high magnesium-to-lithium ratios. This Review explores the latest advances and continuing challenges in lithium extraction from these demanding yet promising sources, covering a variety of methods, including precipitation, solvent extraction, sorption, membrane-based separation and electrochemical-based separation. Furthermore, we share perspectives on the future development of lithium extraction technologies, framed within the basic principles of separation processes. The aim is to encourage the development of innovative extraction methods capable of making use of the substantial potential of low-quality brines.

Prospects in the Use of Cannabis sativa Extracts in Nanoemulsions.

Cannabis sativa plants have been widely investigated for their specific compounds with medicinal properties. These bioactive compounds exert preventive and curative effects on non-communicable and infectious diseases. However, C. sativa extracts have barely been investigated, although they constitute an affordable option to treat human diseases. Nonetheless, antioxidant, antimicrobial, and immunogenicity effects have been associated with C. sativa extracts. Furthermore, innovative extraction methods in combination with nanoformulations have been proposed to increase desirable compounds' availability, distribution, and conservation, which can be aided by modern computational tools in a transdisciplinary approach. This review aims to describe available extraction and nanoformulation methods for C. sativa, as well as its known antioxidant, antimicrobial, and immunogenic activities. Critical points on the use of C. sativa extracts in nanoformulations are identified and some prospects are envisaged.

Pulsed electric field combined with preheating to preserve mildly extracted pea protein

Innovative extraction methods involving dry fractionation and aqueous phase separation have been recently developed to obtain plant-based protein ingredients with high texturing functionality. Such a process was applied to yellow peas, and the microbial quality of the extract was assessed. Pseudomonas trivalis and Erwinia gerundensis were identified as critical microbial contaminants due to their high growth capacities (at 10 °C). As a preservation strategy, pulsed electric field (PEF) treatment (24 kV/cm, 50 Hz, 126 μs) was employed, combined with different product inlet temperatures (25–45 °C). Ohmic heating (ΔT = 20 °C) was measured. At 25–30 °C, the microbial reduction reached 3 logs, attributed solely to electrical effects. At 40 °C, the reduction exceeded 4 logs, where thermal effects dominated. Further increase to 45 °C resulted in thermal modification of pea proteins, potentially compromising its gelling capacity.Additionally, the growth of PEF-treated contaminants at 5 and 10 °C were monitored, revealing rapid cell adaptation and proliferation. Finally, the extracts native microbiota showed an extension of the lag phase (4 days at 5 °C) after PEF treatment, accompanied by a pH stability of 10 days. The findings indicate improved microbial stability of the pea extract following PEF treatment.

Biowaste Transformation to Functional Materials: Structural Properties, Extraction Methods, Applications, and Challenges of Silk Sericin

AbstractThis review underscores the novelty of silk sericin, often regarded as a byproduct in silk production. Comprising approximately 30% of silk, sericin possesses valuable properties that have been largely overlooked in favor of silk fibroin. This work focuses on innovative extraction methods to convert sericin, typically considered waste, into a valuable resource. By examining these extraction techniques alongside diverse applications, the review aims to enhance the recognition and utilization of silk sericin in research and industry. Various extraction processes where the traditional methods, and some new techniques, are all discussed. In detail, the advantages, limitations, and strategy optimization associated with each extraction method are highlighted. For instance, silk sericin has shown promise as a wound treatment agent, a scaffold for tissue engineering, a carrier for drug delivery, and a biomaterial for regenerative medicine in the field of biomedical science due to its biocompatibility, biodegradability, and nontoxicity. Additionally, it also finds application in the cosmetic industry, where it is used in skincare products due to its moisturizing, antioxidant, and antiaging properties. Eventually, the challenges, limitations, and prospects of silk sericin are intensively discussed. This comprehensive review is expected to serve the growing body of knowledge surrounding silk sericin and foster further research and development of silk sericin‐related fields.

Advanced Extraction Techniques and Physicochemical Properties of Carrageenan from a Novel Kappaphycus alvarezii Cultivar.

Carrageenans are valuable marine polysaccharides derived from specific species of red seaweed (Rhodophyta) widely used as thickening and stabilizing agents across various industries. Kappaphycus alvarezii, predominantly cultivated in tropical countries, is the primary source of kappa-carrageenan. Traditional industrial extraction methods involve alkaline treatment for up to three hours followed by heating, which is inefficient and generates substantial waste. Thus, developing improved extraction techniques would be helpful for enhancing efficiency and reducing environmental impacts, solvent costs, energy consumption, and the required processing time. In this study, we explored innovative extraction methods, such as ultrasound-assisted extraction (UAE) and supercritical water extraction (SFE), together with other extraction methods to produce kappa-carrageenan from a new strain of K. alvarezii from the Philippines. FTIR-ATR spectroscopy was employed to characterize the structure of the different carrageenan fractions. We also examined the physicochemical properties of isolated phycocolloids, including viscosity, and the content of fatty acids, proteins, and carbohydrates. For refined carrageenan (RC), both the traditional extraction method and the UAE method used 1 M NaOH. Additionally, UAE (8% KOH) was employed to produce semi-refined carrageenan (SRC). UAE (8% KOH) produced a high yield of carrageenan, in half the extraction time (extraction yield: 76.70 ± 1.44), and improved carrageenan viscosity (658.7 cP), making this technique highly promising for industrial scaling up. On the other hand, SFE also yielded a significant amount of carrageenan, but the resulting product had the lowest viscosity and an acidic pH, posing safety concerns as classified by the EFSA's re-evaluation of carrageenan as a food additive.

Revealing the Potential of Star Anise Essential Oil: Comparative Analysis and Optimization of Innovative Extraction Methods for Enhanced Yield, Aroma Characteristics, Chemical Composition, and Biological Activities.

Star anise has been used for a long time in improving human health and curing diseases, owing to its unlimited components with complex chemical structures and a wide range of bioactivities. This study is aimed to investigate the influence of extraction methods (steam distillation, ethanol Soxhlet extraction, supercritical carbon dioxide extraction, subcritical n-butane extraction) on the yield, aroma properties, chemical composition, and bioactivity of star anise essential oils. Electronic nose detection revealed the essential oils from subcritical extraction exhibited the most intense aroma, while the essential oils from ethanol Soxhlet extraction had a more complex aroma profile. Fourier-transform infrared analysis showed the presence of benzene rings, carbonyl groups, C=C, and aromatic ether bonds in the essential oils extracted through different methods. The major components were heterocyclic olefins, heterocyclic oxygenates, and aromatic oxygenates, as well as certain amounts of flavonoids and polyphenols. Correlation analysis revealed the relative contents of volatile trans-anethole, wormwood, d-limonene, cineole, and trans-α-citronelene were strongly associated with the antibacterial activity of the essential oils. Similarly, the contents of volatile components (d-limonene, cineole) and non-volatile components (total flavonoids and total polyphenols) were strongly correlated with the DPPH scavenging activity of the essential oils. These results confirm the effectiveness of the ethanol Soxhlet extraction method in retaining the bioactivity of the essential oils. Finally, with a Box-Behnken central composite design of response surface and single-factor experiments, the optimal extraction conditions for the ethanol Soxhlet method were determined: ultrasonic frequency of 80 kHz, crushing particle size of 60 meshes, liquid-material ratio of 8:1 (mL:g), and ultrasonic time of 35 min. Under these conditions, the essential oil yield was 25.51% ± 0.21%. Overall, these findings highlight the significance of extraction methods in obtaining high-quality star anise essential oils with desirable aroma properties and potent bioactivities.

Unlocking sustainable lithium: A comparative life cycle assessment of innovative extraction methods from brine

The surging demand for lithium, driven by the widespread adoption of electric vehicles and renewable energy storage systems, underscores the urgent need to develop sustainable lithium extraction methods. This study presents a comprehensive Life Cycle Assessment Using the TRACI method to evaluate and compare the environmental impacts of solvent extraction, adsorption, nanofiltration, and membrane electrolysis as direct lithium extraction methods for recovering lithium from brine to produce lithium carbonate. In terms of global warming, the carbon dioxide emission for each process was determined as follows: solvent extraction emits 52.7 kg CO2eq/kg of lithium carbonate, adsorption emits 47.9 CO2eq/kg of lithium carbonate, nanofiltration emits 17.7 kg CO2eq/kg of lithium carbonate, and membrane electrolysis emits 80.57 kg CO2eq/kg of lithium carbonate. As a result, the nanofiltration process emerges as the most environmentally friendly method, offering a promising solution to the environmental challenges of lithium extraction. In contrast, the membrane electrolysis process has the highest environmental impact.

Deep eutectic solvent strategy for green extraction of chlorogenic acid from sea buckthorn: optimization and sustainability

BackgroundSea buckthorn (Hippophae rhamnoides), a deciduous species plant, is widely distributed around the globe, and native to the cold-temperate regions of Europe and Asia. This medicinal herb contains several bioactive constituents including chlorogenic acid. The conventional methods used for the extraction of phenolic antioxidants from natural herbs often result in low yields, high toxicity, and pose environmental hazards limiting their effectiveness and scalability. Therefore, green extraction techniques using deep eutectic solvents, composed of natural, non-toxic, and biodegradable components were applied for extraction of chlorogenic acid from sea buckthorn weed. Fourteen deep eutectic solvent mixtures were prepared and evaluated for extraction yield of chlorogenic acid. Parameters such as hydrogen bond donor-to-hydrogen bond acceptor ratio, liquid-to-solid ratio, shaking speed, and shaking time were optimized for the best mixture.ResultsThe combination of lactic acid and maltose (1:1) was found to give best extraction yield using response surface methodology. The deep eutectic solvent system under optimum conditions produced 12.2 g/100 g of crude extract sea buckthorn containing 174.7 mg gallic acid equivalents (mg GA)/g) of extract. Moreover, the optimized extract exhibited appreciable radical scavenging capacity (91%), trolox equivalent antioxidant capacity (11.2% of extract), and inhibition of peroxide in linoleic acid (80.6%). High-performance liquid chromatography-based characterization revealed the extracts contained chlorogenic acid (20.1 mg/g of extract) as the major constituent.ConclusionsIn summary, the adoption of DES for the extraction of bioactive phenolic constituents from sea buckthorn offers multiple benefits, including economic efficiency, enhanced extraction performance, and environmental sustainability. The findings of this study not only advance the understanding of DES in phytochemical extraction but also pave the way for broader application of green solvents in the natural products industry. Future research should focus on further optimizing DES formulations and scaling up the extraction process to fully realize the potential of this innovative extraction method in commercial applications.

Resveratrol: Challenges and prospects in extraction and hybridization with nanoparticles, polymers, and bio-ceramics.

Resveratrol (RSV), a bioactive natural phenolic compound found in plants, fruits, and vegetables, has garnered significant attention in pharmaceutical, food, and cosmetic industries due to its remarkable biological and pharmacological activities. Despite its potential in treating various diseases, its poor pharmacokinetic properties, such as low solubility, stability, bioavailability, and susceptibility to rapid oxidation, limit its biomedical applications. Recent advancements focus on incorporating resveratrol into innovative materials like nanoparticles, polymers, and bio-ceramics to enhance its properties and bioavailability. In this review, an exhaustive literature search was conducted from PubMed, Google Scholar, Science Direct, Scopus, and Web of Science databases to explore these advancements, to compares conventional and innovative extraction methods, and to highlights resveratrol's therapeutic potential, including its anti-inflammatory, anti-oxidative, anti-cancerogenic, antidiabetic, neuroprotective, and cardio-protective properties. Additionally, we discuss the challenges and prospects of hybrid materials combining resveratrol with nanoparticles, polymers, and bio-ceramics for therapeutic applications. Rigorous studies are still needed to confirm their clinical efficacy.

A Comprehensive Review on Advanced Extraction Techniques for Retrieving Bioactive Components from Natural Sources.

The extraction of bioactive components from natural sources has gained significant attention in recent years due to increasing demand for natural and functional constituents in various industries, including pharmaceuticals, food, and cosmetics. This review paper aims to provide a comprehensive overview of the studies on extracting bioactive components from natural sources using different advanced extraction techniques. It highlights the need for efficient extraction methods to preserve these components' integrity and bioactivity. Various extraction techniques as supercritical-fluid extraction, microwave-assisted extraction, ultrasound-assisted extraction, subcritical solvent extraction, and solid-phase microextraction are explored in detail, highlighting their principles, advantages, and limitations. The review further examines the impact of different factors on the extraction process, including solvent selection, extraction time, temperature, ultrasonication-amplitude, etc. Additionally, emerging techniques, such as green extraction methods and nanotechnology-based approaches, are discussed, emphasizing their potential to enhance the extraction efficiency and sustainability of the process. Furthermore, the review presents case studies and experimental results from recent research articles, providing insights into applying different extraction techniques for specific bioactive components, such as phenolics, flavonoids, alkaloids, and essential oils. It discusses the extraction yield, bioactivity, and potential utilization of the extracted components in various industries. Overall, this review paper is valuable for researchers, scientists, and industry professionals interested in extracting bioactive components from natural sources. It consolidates the current knowledge on different advanced extraction techniques, their optimization parameters, and their potential applications, facilitating further advancements in the field and the development of innovative extraction methods for bioactive component extraction from natural sources.

Assessing the functional and physicochemical properties of chia seed mucilage extracted using an innovative extraction method

Chia seed mucilage (CSM) is a novel, eco-green, and sustainable edible hydrocolloid derived from chia, offering a promising alternative to commercial hydrocolloids. This study explores the application of centrifugal force and drying methods to compare the techno-functionalities of CSM obtained through a novel, faster, and scalable extraction approach as opposed to traditional methods. Liquid mucilage extracted using a modified cream separator is subjected to freeze-drying (CSMF) and oven-drying (CSMO). The efficiency of this innovative extraction method is compared to the traditional dry fractionation approach, known for its complexity and time consumption. Remarkably, the modified unit significantly (p<0.05) reduces mucilage separation time (4.06 ± 1.28 min), opening doors for future commercialization. CSMF obtained through cream separator and freeze-drying, exhibits superior colour properties. Additionally, it demonstrates superior performance compared to the traditional approach in terms of functional properties such as water-holding capacity (WHC), solubility, and particle size. Furthermore, the advantages of the faster extraction approach are reinforced through charge analysis, Fourier Transform Infrared spectra (FTIR), thermal transitions, and rheological properties. The microstructure analysis of CSM highlights the influence of drying techniques on its morphology. This study not only introduces a novel method for mucilage extraction but also validates the findings through comparison with existing literature on CSM.

Green Propolis: A Review of Pharmaceutical Patents with Potential Therapeutic Applications

Abstract: Green propolis is a resin produced from Baccharis dracunculifolia, which has as its main compounds flavonoids, derivatives of cinnamic acids, such as artepillin C and baccarin. This resin has antibacterial, antifungal, anti-inflammatory, antioxidant, and anticancer activities. This review aimed to analyze pharmaceutical patents containing green propolis in various formulations. The search was conducted in the National Institute of Industrial Property (INPI), the Patent Search of Spain and Latin America (Latipat-Espacenet), the World Intellectual Property Organization (WIPO), and Google Patents, with a combination of the keywords green propolis, green propolis extract, pharmacology, and pharmaceutical product. Primary research identified 60 patents, from which 22 were selected after applying the inclusion criteria. The selected patents referred to products with pharmacological activities, from cancer treatment to food supplements and included innovations for improved controlled release of the green propolis compounds. Most of the documents concerned the preparation and/or formulation of the green propolis extract, followed by innovative extraction methods, treatment and systemic use, and finally by topical use and quality control techniques and procedures. Thus, the reviewed patents of green propolis provided valuable insights into the pharmaceutical applications of green propolis, showing its potential in diverse formulations and treatments.

Sustainable Valorization of Coffee Silverskin: Extraction of Phenolic Compounds and Proteins for Enzymatic Production of Bioactive Peptides.

Coffee silverskin (CS), a by-product of the coffee roasting process, has high protein content (16.2-19.0%, w/w), making it a potential source for plant protein and bioactive peptide production. This study aims to develop innovative extraction methods for phenolic compounds and proteins from CS. The conditions for hydrothermal (HT) extraction of phenolic compounds from CS were optimized by varying CS loading (2.5-10%, w/v), temperature (110-130 °C), and time (5-30 min) using a one-factor-at-a-time (OFAT) approach. The highest TPC of 55.59 ± 0.12 µmole GAE/g CS was achieved at 5.0% (w/v) CS loading and autoclaving at 125 °C for 25 min. Following hydrothermal extraction, CS protein was extracted from HT-extracted solid fraction by microwave-assisted alkaline extraction (MAE) using 0.2 M NaOH at 90 W for 2 min, resulting in a protein recovery of 12.19 ± 0.39 mg/g CS. The CS protein was then subjected to enzymatic hydrolysis using protease from Bacillus halodurans SE5 (protease_SE5). Protease_SE5-derived CS protein hydrolysate had a peptide concentration of 0.73 ± 0.09 mg/mL, with ABTS, DPPH, and FRAP values of 15.71 ± 0.10, 16.63 ± 0.061, and 6.48 ± 0.01 µmole TE/mL, respectively. Peptide identification by LC-MS/MS revealed several promising biological activities without toxicity or allergenicity concerns. This study's integrated approach offers a sustainable and efficient method for extracting valuable compounds from CS, with potential applications in the food and pharmaceutical industries.

Optimization of Ultrasound Assisted Extraction Conditions on Fingerroots (Boesenbergia rotunda) Rhizome and its Antioxidant Activity

Fingerroot scientifically known as Boesenbergia rotunda, is a widespread herb in Southeast Asia and South Asia. This experiment was conducted to investigate the effect of temperature (40 to 60 °C) and time (30 to 60 min) of the fingerroot extract yield and its antioxidant activity using Ultrasound-Assisted Extraction (UAE). The study compared the performance of UAE with the traditional maceration method in terms of extract yield and antioxidant activity. UAE, recognized as a more advanced and innovative extraction method, demonstrated superior efficiency in terms of being time-saving, cost-effective, and faster than maceration. The optimized UAE conditions were identified at an extraction temperature of 48 °C for 42.1 minutes, resulting in an extract yield of 8.42% and an antioxidant activity of 85.90%. In conclusion, the study affirms that UAE offers a highly effective and efficient approach for extracting valuable compounds from fingerroot, showcasing its potential for applications in various industries.

Abstract Book ICONAHE 2023

The conference was held on November 26-28, 2023 in Abdelhamid Ibn Badis University, Mostaganem, Algeria, which will be organized by pharmacognosy &amp;Api phytotherapy laboratory.&#x0D; The scientific program covered all related aspects of medicinal plants in the following main topics: Using medicinal plants and their extracts in the pharmacological activities, Phytotherapy, Natural products, biological activity and Toxicity, Ethnobotany, and innovative extraction methods, Importance medicinal and environmental socioeconomic medicinal plants at the national and international scale, Apitherapy, Medicinal plant in ethno-veterinary practice and animal nutrition. Participants more than twenty countries met and planned further collaborations each other.

Recent Development on the Extraction Process of Plants Essential Oil and its Effect on Chemical Composition: A Review

This study presents a thorough review on different innovative extraction techniques of different plant essential oil derived from its peel, leaves, seeds and shrubs. The review also entails the different pre-treatment processes that affects the quality and recovery of the plant essential oil. Also, discussed the recently developed green extraction method such as Microwave Assisted Hydrodistillation (MAHD), Solvent Free Microwave Extraction (SFME), Ultrasound Assisted Extraction (UAE), Supercritical Fluid Extraction (SFE) and Ohmic Heated Assisted Hydrodistillation (OHAD). The impact of these extraction process on the important chemical composition of essential oil is further analyzed in this review. The important parameters in extraction is also studied such as the water to material ratio, extraction temperature, power input and extraction time. Based on the different articles reviewed, it signifies that the innovative extraction method improves the quality and quantity of the essential oil yield. It also improved the number of component that can be extracted in the plant essential oil and its biological activities such as antioxidant and antimicrobial activities. Thus this review aims to emphasize the potential of the newly developed essential oil extraction techniques that would help to boon the pharmaceuticals, food and agriculture industry.

Innovative methods for extraction of essential oils from medicinal plants

Essential oils are concentrated liquids of intricate combinations of volatile substances, extracted from various plant parts. Several bioactive substances with antibacterial and antioxidant activities are abundant in essential oils. Some essential oils have also been employed in medicine. Due to the risk associated with employing synthetic preservatives, the use of essential oils as natural additives for extending the shelf life of food products has also drawn considerable attention. They are used in the pharmaceutical, cosmetic, and food industries for their functional properties. There are various methods for extraction, but both the quality and the percentage yield of essential oil never remain the same. So, innovative and non-conventional techniques of essential oils extraction from medicinal plants were evoled to get quantitative and qualitative yield. In the present article, we searched and reviewed innovative techniques used for the extraction of essential oils from medicinal and aromatic plants through electronic searches of PubMed, Medline, Wiley, Scopus, and Google Scholar. For the extraction of essential oils, several innovative/non-conventional techniques have been reported in literature. Extraction of essential oil by using innovative techniques retards the risk of losing the essential components of plants, maintains the quality, reduces chemical risk, extraction time, acts eco-friendly, and increases the percentage yield of the essential oils. This paper presents the success story of innovative extraction methods of essential oils in accordance with sustainable development and environmental protection.

Fruits as nutraceuticals: A review of the main fruits included in nutraceutical patents

Fruits have relevant usefulness in the elaboration of nutraceutical compositions and, as it is considered a “natural medicine”, its market has been growing exponentially each year. Fruits, in general, contain a large source of phytochemicals, carbohydrates, vitamins, amino acids, peptides and antioxidants that are of interest to be prepared as nutraceuticals. The biological properties of its nutraceuticals can range from antioxidant, antidiabetic, antihypertensive, anti-Alzheimer, antiproliferative, antimicrobial, antibacterial, anti-inflammatory, among others. Furthermore, the need for innovative extraction methods and products reveals the importance of developing new nutraceutical compositions. This review was developed by searching patents of nutraceuticals from January 2015 until January 2022 in Espacenet, the search database of the European Patent Office (EPO). Of 215 patents related to nutraceuticals, 43% (92 patents) were including fruits, mainly berries. A great number of patents were focused on the treatment of metabolic diseases, representing 45% of the total patents. The principal patent applicant was the United States of America (US), with 52%. The patents were applied by researchers, industries, research centers and institutes. It is important to highlight that from 92 fruit nutraceutical patent applications reviewed, 13 already have their products available on the market.