In the era of knowledge economy, market competition is constantly changing, and enterprises are developing rapidly in China. With the strong support of the Chinese government for innovation and entrepreneurial activities, the entrepreneurial development situation is optimistic,especially as the main business enterprises to enter the stage of rapid development of youth entrepreneurship.However, while having opportunities, we can also see that the global economic competition environment is complex.Chinese youth entrepreneurs still need to improve their competitiveness and promote the development of innovative technologies. It is necessary for employees to continuously absorb knowledge culture and update their innovative technologies. This paper mainly studies the relationship between organizational culture, organizational learning and innovation performance of young entrepreneurs. 302 questionnaires were collected from Chinese entrepreneurial enterprises, and we uses SPSS and AMOS to analyze organizational culture, organizational learning and innovation performance, and shows the organizational culture affects innovation performance through organizational learning. Finally, in order to make a good organizational culture, enterprises need to continuously update their knowledge,improve the soft power of enterprises, to cope with the changing market environment, enhance the creativity of enterprises and their innovation performance.