Innate Potential Research Articles

Wilckodontics: The Periodontal Orthodontics

AbstractPeriodontally accelerated osteogenic orthodontics (PAOO), also known as Wilckodontics, is a clinical procedure that combines corticotomy (a surgical technique in which the bone is cut, perforated, or mechanically altered), particulate bone grafting, and orthodontic force application. By this procedure, the teeth can be made to move through the bone rapidly by means of harnessing and stimulating the innate potential of the bone and utilizing tissue engineering principles. Once the tooth movement gets completed, bone rebuilds around the tooth, thereby reducing the time of orthodontic treatment from years to months. This article aims to present a comprehensive review about PAOO or Wilckodontics.

Relationship Between Emotional Intelligence and Reading Comprehension Ability and Vocabulary Knowledge of Iranian EFL Learners in Tehran Azad Universities

The purpose of the present study was to determine whether EQ, as an interpersonal skill and innate potential, had any relationship between reading comprehension and Vocabulary Knowledge of learners. To this end, 150 Iranian EFL learners studying at Islamic Azad University (IAU) took an Oxford Placement Test (OPT); 150 candidates (95 males and 55 females) who were at the intermediate level were selected as the participants of the study and hence took the self-report measure Emotional Intelligence Quotient Quiz. The validity and reliability of EQi were measured and the instrument was found to be valid and highly internally consistent. Correlation and regression analysis were performed to test the hypotheses. Results revealed that there was not a strong relationship between Emotional Intelligence and EFL learners’ reading comprehension and vocabulary knowledge.

Talent Management: The India Way

Talent can be defined as performance minus effort such that a more talented person will perform better than an equally motivated but less talented peer because of their innate ability or potential. Talent management may be understood as all that practices that lead to attracting, developing and retaining people with the capabilities of meeting current and future organisational needs. Talent management practices in any nation or region would give best results when they are in consonance with the culture of that nation or region. Culture and environment fit matters even as extremely talented individual will not reach full potential in an environment that is a poor fit. One of the most comprehensive studies on how values in workplace are influenced by culture done by Hofstede, six dimensions of culture were identified. As per Hofstede, the interpretation of cultural dimensions for India was as follows: High Power Distance, High Long-Term Orientation, High individualism, High Masculinity, Low on Uncertainty Avoidance and Low on Indulgence. Sycophancy and exaggerated outward respect for superiors and seniors as characteristic part of Indian corporate life and in ‘cultural DNA’ of India were identified. India's tolerance of diversity has also been found to be one of the greatest potential gifts to the world culture. Despite the in-group out-group dichotomy existing there has been a high tolerance for diversity in India. Talent management in India is characterised by removing obstacles such as lack of resources through ‘jugaad’. Therefore, the challenge lies for HR professionals in understanding cultural DNA of India as well as working towards integrating the functional elements of different countries and regions present in India and adapting them to Indian conditions and managing and developing talent in the long run. Paper discusses how talent can be managed effectively, through a model proposed by authors, which explores Indian cultural context and fit of employees within the context for best results.

Isolation, Identification and Characterization of marine bacteria from the deep sea sediment of Bay of Bengal, India, and their antimicrobial and cytotoxicity potential.

A total of 108 marine bacteria were isolated from the deep sea sediment of Bay of Bengal, India. Of which, 15 bacteria showed antimicrobial activity against human pathogenic bacteria. These antagonistic marine bacteria were characterized phenotypically and their taxonomic affiliations were made on the basis of 16S rRNA gene homology and molecular phylogeny tree analysis, the antagonistic marine bacteria were identified as the species of Bacillus, Halobacillus, Staphylococcus, Marinobacter. The extracts of potent marine bacteria exhibited differential cytotoxicity towards lung (A549), cervical (HeLa), breast (MCF-7) and colon (HT-29) cancer cells. The acridine-orange and ethidium bromide (AO/EB) staining of the extract of strain MB30-treated cancer cells showed typical characteristics of apoptosis such as nuclear condensation, cell shrinkage and formation of apoptotic bodies. Present investigation, reports potent marine bacteria from the deep sea sediment of Bay of Bengal that exhibit broad-spectrum antimicrobial and cytotoxicity potential. Due to their innate bioactive potential, these bacteria can be used as the source of potent molecules.

Random-sequence genetic oligomer pools display an innate potential for ligation and recombination.

Recombination, the exchange of information between different genetic polymer strands, is of fundamental importance in biology for genome maintenance and genetic diversification and is mediated by dedicated recombinase enzymes. Here, we describe an innate capacity for non-enzymatic recombination (and ligation) in random-sequence genetic oligomer pools. Specifically, we examine random and semi-random eicosamer (N20) pools of RNA, DNA and the unnatural genetic polymers ANA (arabino-), HNA (hexitol-) and AtNA (altritol-nucleic acids). While DNA, ANA and HNA pools proved inert, RNA (and to a lesser extent AtNA) pools displayed diverse modes of spontaneous intermolecular recombination, connecting recombination mechanistically to the vicinal ring cis-diol configuration shared by RNA and AtNA. Thus, the chemical constitution that renders both susceptible to hydrolysis emerges as the fundamental determinant of an innate capacity for recombination, which is shown to promote a concomitant increase in compositional, informational and structural pool complexity and hence evolutionary potential.

Accurate measurement of electric potentials in biased GaAs compound semiconductors by phase-shifting electron holography.

The innate electric potentials in biased p- and n-type GaAs compound semiconductors and the built-in potential were successfully measured with high accuracy and precision by applying in situ phase-shifting electron holography to a wedge-shaped GaAs specimen. A cryo-focused-ion-beam system was used to prepare the 35°-wedge-shaped specimen with smooth surfaces for a precise measurement. The specimen was biased in a transmission electron microscope, and holograms with high-contrast interference fringes were recorded for the phase-shifting method. A clear phase image around the p-n junction was reconstructed even in a thick region (thickness of ~700 nm) at a spatial resolution of 1 nm and precision of 0.01 rad. The innate electric potentials of the unbiased p- and n-type layers were measured to be 12.96 ± 0.17 V and 14.43 ± 0.19 V, respectively. The built-in potential was determined to be 1.48 ± 0.02 V. In addition, the in situ biasing measurement revealed that the measured electric-potential difference between the p and n regions changed by an amount equal to the voltage applied to the specimen, which indicates that all of the external voltage was applied to the p-n junction and that no voltage loss occurred at the other regions.

Patient-Centered Outcomes in Bladder Cancer.

To summarize current knowledge on patient-prioritized outcomes for their bladder cancer care. Patient-centered outcomes research seeks to help patients identify the right treatment for the right patient at the right time in their care. As such, patient-centered outcomes research relies on studying a treatment's impact on patient-centered outcomes. Some outcomes, like survival, are commonly prioritized by patients and by clinical experts. Patients often place greater emphasis than experts on quality of life outcomes. Thus, many patient-centered outcomes are also patient-reported outcomes. Unique domains that are often prioritized by patients, but overlooked by experts, include the costs and financial impact of care, anxiety, and depression related to a health condition, and the impact of a condition or its treatment on a caregiver or loved one. Patient-centered outcomes are realizing greater recognition for their innate importance and potential to augment the impact of research studies. Although patient-centered outcomes are often patient-reported outcomes, this is not universal. Unique to bladder cancer, the availability of a research-oriented Patient Survey Network intended to identify research questions that are important to patients may be an opportunity to broadly solicit input on patient-centered outcomes for bladder cancer research questions.

Tendon stem progenitor cells: Understanding the biology to inform therapeutic strategies for tendon repair.

Tendon and ligament injuries are a leading cause of healthcare visits with significant impact in terms of economic cost and reduced quality of life. To date, reparative strategies remain largely restricted to conservative treatment or surgical repair. However, these therapies fail to restore native tendon structure and function; thus, the tissue may re-rupture or degenerate with time. To improve tendon healing, one promising strategy may be harnessing the innate potential of resident tendon stem/progenitor cells (TSPCs) to guide tenogenic regeneration. In this review, we outline recent advances in the identification and characterization of putative TSPC populations, and discuss biochemical, biomechanical, and biomaterial methods employed for their culture and differentiation. Finally, we identify limitations in our current understanding of TSPC biology, key challenges for their use, and potential therapeutic strategies to inform cell-based tendon repair. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:1270-1280, 2019.

Cytotoxicity Activity of Crude Extract Produced by Marine Isolate (MS19) from Sea Water collected from Bay of Bengal, India

Marine microbes comprises approximately a half of the total global biodiversity. Sea offers an enormous resource for novel bioactive compounds and it has been classified as the largest remaining reservoir of natural molecules to be evaluated for drug activity. Marine bacteria have been received attention of researchers due to their innate potential to produce diverse compounds that attracts biological properties such as anticancer, anti-inflammatory, antimicrobial and antioxidant properties. In this study, we reported isolation of 10 strains from sea water and tested them with standard biochemical test and their potential to utilize carbon sugar, to confirm the Actinomycetes character. Among the isolates 10 and 1 was recorded to be gram positive. Carbon utilization test revealed that all the isolates utilized dextrose and none of the isolates utilized ducitol. Strain MS19 a non-motile, catalase positive, oxidase positive, and round shape strain was isolated from sea water of the Bay of Bengal.

Receptor tyrosine kinase MerTK suppresses an allogenic type I IFN response to promote transplant tolerance.

Recipient infusion of donor apoptotic cells is an emerging strategy for inducing robust transplantation tolerance. Daily clearance of billions of self-apoptotic cells relies on homeostatic engagement of phagocytic receptors, in particular, receptors of the tyrosine kinase family TAM (Tyro3, Axl, and MerTK), to maintain self-tolerance. However, an outstanding question is if allogeneic apoptotic cells trigger the same receptor system for inducing allogeneic tolerance. Here, we employed allogeneic apoptotic splenocytes and discovered that the efferocytic receptor MerTK on recipient phagocytes is a critical mediator for transplantation tolerance induced by this strategy. Our findings indicate that the tolerogenic properties of allogeneic apoptotic splenocytes require MerTK transmission of intracellular signaling to suppress the production of inflammatory cytokine interferon α (IFN-α). We further demonstrate that MerTK is crucial for subsequent expansion of myeloid-derived suppressor cells and for promoting their immunomodulatory function, including maintaining graft-infiltrating CD4+ CD25+ Foxp3+ regulatory T cells. Consequently, recipient MerTK deficiency resulted in failure of tolerance by donor apoptotic cells, and this failure could be effectively rescued by IFN-α receptor blockade. These findings underscore the importance of the efferocytic receptor MerTK in mediating transplantation tolerance by donor apoptotic cells and implicate MerTK agonism as a promising target for promoting transplantation tolerance.

Photoactivated [Mn(CO)3Br(μ-bpcpd)]2 induces apoptosis in cancer cells via intrinsic pathway

Carbon monoxide releasing molecules (CORMs) are organometallic/organic compounds that release carbon monoxide (CO) spontaneously or upon activation. PhotoCORMs are capable of releasing CO on light based activation. This group of molecules is used in photodynamic therapy due to their ability to release CO in a controlled manner. In the present investigation, the release of CO from [Mn(CO)3Br(μ-bpcpd)]2 (MnCORM) upon irradiation at λmax 365 nm was assessed spectrophotometrically using myoglobin assay and confirmed by liquid FT-IR spectroscopic analysis. Further, the cytotoxic potential of MnCORM on normal cells (HEK 293) and cancer cell lines such as lung (A549), cervical (HeLa), breast (MDA MB-231) and colon (HCT-15) was evaluated. The IC50 values of MnCORM were found to be 21.37 ± 1.72, 24.12 ± 1.03, 21.89 ± 0.59 and 13.69 ± 0.91 μM on cervical (HeLa), lung (A549), colon (HCT-15) and breast (MDA MB-231) cancer cells respectively. An inquest into the nature of cell death was confirmed based on the nuclear and cytological examinations, flow cytometric analyses and protein expression studies. The AO/EB dual staining and cytological evaluation of the treated cells revealed that the cell death might be due to apoptosis. The flow cytometric analysis of propidium iodide (PI) stained cells showed a significant amount of sub-G1 hypodiploid cells due to MnCORM treatment. The MnCORM-induced apoptosis was mediated through the generation of reactive oxygen species (ROS), specifically superoxide radicals leading to loss of mitochondrial membrane potential. The intrinsic pathway of apoptosis was elucidated based on the expression studies of pro-apoptotic and apoptotic proteins such as bcl-2, bax, cyt c, cleaved caspase-3, cleaved caspase-9 and cleaved PARP. Due to its innate potential to release CO upon photoactivation and its ability to induce apoptosis via intrinsic pathway, the MnCORM molecule could be exploited for controlled release and photodynamic cancer therapy.

LAYANAN KONSELING UNTUK REMAJA DALAM MEMBANTU KEBIASAAN BERMAIN GAME SERTA PERILAKU SEKS BEBAS


 
 
 
 
 
 Adolescence is the most beautiful period. Adolescence must be saved from the era of globalization. Because globalization is like a freedom, so many foreign cultures enter, though the culture is not compatible with Indonesian culture. The younger generation is the backbone of the nation. They are expected to be able to continue the leadership of this nation for the better in the future.in preparing the younger generation also depends on the readiness of the community, that is with the existence of the culture. Thecnology is becoming so close to humans, especially teenagers. This is because technology can facilitate people in doing things. However, technology is often misused to open sites that should not be opened by teenagers. Teenagers often feel curious at sites that result in sex outside of marriage. Through guidance and counseling services in an effort to solve client problems, so counselor should be able to understand and develop every motive and motivation underlying the behavior of clients. In addition, a counselor should be able to identify the client’s innate potential. This is useful for counselors in creating a conducive environtment for clients.
 Keywords :, gadget, counseling services, teenagers, free sex
 
 
 
 
 

Evaluation of the innate immunostimulatory potential of originator and non-originator copies of insulin glargine in an in vitro human immune model.

BackgroundThe manufacture of insulin analogs requires sophisticated production procedures which can lead to differences in the structure, purity, and/or other physiochemical properties of resultant products that can affect their biologic activity. Here, we sought to compare originator and non-originator copies of insulin glargine for innate immune activity and mechanisms leading to differences in these response profiles in an in vitro model of human immunity.MethodsAn endothelial/dendritic cell-based innate immune model was used to study antigen-presenting cell activation, cytokine secretion, and insulin receptor signalling pathways induced by originator and non-originator insulin glargine products. Mechanistic studies included signalling pathway blockade with specific inhibitors, analysis of the products in a Toll-like receptor reporter cell line assay, and natural insulin removal from the products by immunopurification.FindingsAll insulin glargine products elicited at least a minor innate immune response comparable to natural human insulin, but some lots of a non-originator copy product induced the elevated secretion of the cytokines, IL-8 and IL-6. In studies aimed at addressing the mechanisms leading to differential cytokine production by these products, we found (1) the inflammatory response was not mediated by bacterial contaminants, (2) the innate response was driven by the native insulin receptor through the MAPK pathway, and (3) the removal of insulin glargine significantly reduced their capacity to induce innate activity. No evidence of product aggregates was detected, though the presence of some high molecular weight proteins argues for the presence of insulin glargine dimers or others contaminants in these products.ConclusionThe data presented here suggests some non-originator insulin glargine product lots drive heightened in vitro human innate activity and provides preliminary evidence that changes in the biochemical composition of non-originator insulin glargine products (dimers, impurities) might be responsible for their greater immunostimulatory potential.

Innate immunity in stem cell-derived hepatocytes

Stem cell-derived hepatocyte-like cells (HLCs) offer great opportunities for studies of host-pathogen interactions and tissue regeneration, as well as hepatotoxicity. To reliably predict the outcome of infection or to enhance graft survival, a finely tuned innate immune system is essential. Hepatocytes have long been considered solely metabolic and their critical innate immune potential is only recently gaining attention. Viral infection studies show that pathogen detection by cytosolic receptors leads to interferon (IFN) induction in primary hepatocytes and HLCs. IFN expression in HLCs is characterized by strong expression of type III IFN and low expression of type I IFN which is also a characteristic of primary hepatocytes. The response to IFN differs in HLCs with lower interferon-stimulated gene (ISG)-expression levels than in primary hepatocytes. Tumour necrosis factor-alpha (TNF-α) signalling is less studied in HLCs, but appears to be functional. Expression of toll-like receptors (TLR) 2-5, 7 and 9 has been reported in primary hepatocytes but has been poorly studied in HLCs. In summary, although they retain some immature features, HLCs are in many ways superior to hepatoma cell lines for cell-based modelling. In this review, we will provide an overview of innate immune signalling in HLCs and how this compares with primary hepatocytes.This article is part of the themed issue 'Designer human tissue: coming to a lab near you'.

Screening of candidate biomaterials for alveolar augmentation using a critical-size rat calvaria defect model.

To screen candidate biomaterials intended for alveolar augmentation relative to their potential to enhance local bone formation using a routine critical-size (ø8-mm) rat calvaria defect model. One hundred and forty male Sprague Dawley outbred rats, age 11-12weeks, weight 325-375g, obtained from USDA approved breeder, randomised into 14 groups of 10 animals, each received one of the following treatments: sham-surgery (empty control), Bio-Oss (bovine HA/reference control), or candidate biomaterials including bovine HA, synthetic HA/ß-TCP and calcium phosphate constructs, mineralised/demineralised human bone preparations, a ß-TCP/calcium sulphate and an HA/calcium sulphate putty. A 4-week healing interval was chosen to discern local bone formation using incandescent and polarised light microscopy. Statistical analysis used one-way ANOVA followed by Bonferroni for pairwise comparisons. Candidate biomaterials all displayed biocompatibility. They exhibited limited, if any, appreciable bioerosion or biodegradation. No statistically significant differences in mean linear defect closure were observed among experimental groups, sham-surgery displaying the highest score (48.1±24.3%). Sham-surgery also showed a significantly greater bone area fraction than all other groups (19.8±13.9%, p<.001). The HA/calcium sulphate putty showed a significantly greater residual biomaterial area fraction than all other groups (61.1±8.5%, p<.01). Within the limitations of this animal model, although biocompatible, none of the tested biomaterials enhanced local bone formation beyond the innate regenerative potential of this craniotomy defect.

Plant seed mucilage as emerging biopolymer in food industry applications

The demand for sustainable, eco-green and minimally processed food ingredients of innate technological (structuring, texturising, stabilising) and functional potential is on the growth. Plant seed mucilage (PSM) constitutes a polysaccharide hydrocolloid of particular physicochemical and structure conformational diversity which endows a broad range of functional and health relevant aspects. Many studies have demonstrated the peculiar structuring and stabilising role of crude and fractionated PSM in model and real food matrices. In addition, PSMs intake through the orogastrointestinal route has been associated with several health benefits such as modulation of postprandial glycaemic and insulinaemic response, hyperlipidaemia counteracting, satiety enhancement, regulation of gut microbiota function. This concise review discusses the most recent advancements in the field of PSM extraction and characterisation as well as their exploitation as alternative hydrocolloids for food and nutraceutical industry applications.

Grace, Symbol, and Liturgy: Constructing the Theological Anthropology of Nichiren Daishonin

Anthony B. Pinn called upon theologians to do theology in "non-traditional ways," by considering overlooked dimensions of African American life and black theology. One way to do this is to consider a non-Abrahamic religious tradition that African Americans practice, such as Nichiren Buddhism. As I am both African American and a Buddhist, failure to consider such overlooked dimensions of African American life and black theology cause people like myself to be excluded. In this article, I examine the writings and liturgical recommendations of Nichiren Daishonin (1222–1282) in order to construct his theological anthropology. Nichiren was a Buddhist monk who publicly broke with the Japanese Tendai tradition in which he had been ordained, by declaring that the best way to express devotion to the teachings contained in the Saddharmapuṇḍarīka Sūtra or The Sūtra on the Lotus Blossom of the Wonderful Law (Lotus Sūtra) was to chant its title in the form of a mantra: Nam-MyōhōRenge-Kyō. I bring Nichiren's writings into dialogue with Karl Rahner's theology of the symbol, as presented by US Hispanic Catholic theologian Miguel Diaz; with feminist theologian Jennifer Beste's work on trauma theory as a challenge to Rahner's theology of grace; and with womanist theologian M. Shawn Copeland's notion of "liturgy of the Spirit." This dialogue shows that for Nichiren, the human being is "Buddha-in-the-world," and has an innate potential to express the life-state of Buddhahood; one primary symbol, the Gohonzon, manifests this potential; and suffering is the result of an innate capacity for "hellish experiences" due to a kind of ignorance known as fundamental darkness. Further, Beste's work on trauma theory and Copeland's notion of "liturgy of the Spirit" show that Nichiren's theological anthropology is concretized in the liturgical practice known as gongyō that he recommended his followers engage in, and demonstrates that Nichiren gives sufficient attention to experiences of brokenness and healing, thereby avoiding the charge of having too positive a theology. [End Page 267]

Immune Training Unlocks Innate Potential

Trained immunity is a form of innate immune memory with distinct features from classical adaptive immune memory. In the current issues of Cell and Cell Host & Microbe, five studies from the International Trained Immunity Consortium shed light on mechanisms and functional consequences of this phenomenon on cellular and whole-organism levels.

Postawy abiturientów wobec rynku pracy. Szczecin i Czerniowce–studium przypadku

Na postawę młodego człowieka wobec rynku pracy ma wpływ grupa indywidualnych czynników związanych z wrodzonym potencjałem intelektualnym i z wychowaniem danej osoby. Niewątpliwy wpływ na zachowania przedsiębiorcze ma także grupa czynników zewnętrznych związanych z otoczeniem społeczno-ekonomicznym, wśród których ważną rolę odgrywa miejsce zamieszkania oraz miejsce edukacji (szkoła). Często niedoceniany pozostaje czynnik historyczno-kulturowy, który wprost – a czasami pośrednio –rzutuje na postawę życiową człowieka (także jego działalność). Celem artykułu było porównanie postaw abiturientów liceów ogólnokształcących pochodzących ze Szczecina oraz ukraińskiego miasta Czerniowce wobec rynku pracy. Przyjęto, że badane grupy mają bardzo zróżnicowane warunki wejścia, a następnie funkcjonowania na rynku pracy, który w obu krajach ma bardzo odmienne uwarunkowania działania, a tym samym oferuje różne możliwości osiągnięcia sukcesu zawodowego dla pracowników. Nie bez znaczenia pozostaje przy tym nieco odmienny model edukacji, w tym wiek abiturientów. Głównym narzędziem pomiarowym była ankieta skierowana do absolwentów wiodących szkół średnich w Szczecinie (Polska) i Czerniowcach (Ukraina), którą przeprowadzano w przekrojowym badaniu bezpośrednim, w okresie od kwietnia do maja 2016 r. Jak wynika z analizy, nie ma zasadniczych różnic pomiędzy postawami abiturientów z Czerniowców i ze Szczecina. Rozbieżności dotyczą jedynie skali podobnych odpowiedzi, co może wynikać z różnicy wieku respondentów, modelu rodziny i zamożności, a przede wszystkim odmiennych środowisk.

Management of undernutrition and failure to thrive in children with congenital heart disease in low- and middle-income countries.

Poor growth with underweight for age, decreased length/height for age, and underweight-for-height are all relatively common in children with CHD. The underlying causes of this failure to thrive may be multifactorial, including innate growth potential, severity of cardiac disease, increased energy requirements, decreased nutritional intake, malabsorption, and poor utilisation of absorbed nutrition. These factors are particularly common and severe in low- and middle-income countries. Although nutrition should be carefully assessed in all patients, failure of growth is not a contraindication to surgical repair, and patients should receive surgical repair where indicated as soon as possible. Close attention should be paid to nutritional support - primarily enteral feeding, with particular use of breast milk in infancy - in the perioperative period and in the paediatric ICU. This nutritional support requires specific attention and allocation of resources, including appropriately skilled personnel. Thereafter, it is essential to monitor growth and development and to identify causes for failure to catch-up or grow appropriately.