The main cause of injury in athletes is of muscular origin and of all those of the hamstrings it is the most important. The inadequate eccentric strength of these is a factor that is related to lower limb injuries. At the Sports Science Center of the Ministry of Sports, the eccentric strength of the hamstrings is evaluated with the Nordic test. In Colombia, it is not known if this is related to lower limb injuries in the different world-class high-performance athletes who attend there. A descriptive work was proposed with a quantitative approach and analytical phase, evaluating the pre-participation medical records of 195 athletes who underwent the Nordic test during the year 2021. The athletes analyzed were 56% men, with ages for both sexes on average of 21.5 years, with body mass indexes of approximately 22.1 k/m2. The most frequent injury to the lower limbs was muscle (38.5%), followed by tendinopathies (27%). Of the muscle, the hamstrings were injured in 69%. An average maximum force was found for all athletes of 292.4 ± 67.06 N and a relative force of 4.52 ± 1 N/kg. The bivariate analyzes show an association between the presence of injury and lower maximum eccentric hamstring strength for both sexes. Furthermore, it was found that asymmetries less than 15% of the maximum eccentric hamstring strength were associated with a lower presence of injury. From a multivariate analysis, normative reference curves were constructed for weight, sex, and maximum eccentric strength of these athletes. It contributes to the conceptual gap of the behavior of eccentric hamstring strength and its relationship with the presence of lower limb injuries in different Colombian elite athletes.