Slurry Injection Research Articles

Ticam2 ablation facilitates monocyte exhaustion recovery after sepsis

Sepsis is a leading cause of death worldwide, with most patient mortality stemming from lingering immunosuppression in sepsis survivors. This is due in part to immune dysfunction resulting from monocyte exhaustion, a phenotype of reduced antigen presentation, altered CD14/CD16 inflammatory subtypes, and disrupted cytokine production. Whereas previous research demonstrated improved sepsis survival in Ticam2-/- mice, the contribution of TICAM2 to long-term exhaustion memory remained unknown. Using a cecal slurry injection sepsis model, we monitored the establishment and recovery of monocyte exhaustion in Ticam2-/- mice. After one week of recovery, we profiled bone marrow and splenic reservoir monocytes in Ticam2-/- mice and found that, in contrast to the persistent exhaustion observed in wild-type monocytes, Ticam2-/- monocytes largely resembled healthy controls. To determine the impact of TICAM2 ablation on innate epigenetic memory in sepsis, we measured genome-wide DNA methylation in bone marrow monocytes and found that Ticam2-/- cells exhibit a unique profile of altered methylation at CEBPE binding sites and regulatory features for key immune genes such as Dmkn and Btg1. Bearing human translational relevance, a case study of time course blood samples collected from a sepsis patient presenting with SIRS and a positive qSOFA revealed a similar effect in human monocytes, which steadily transition into an exhausted memory characterized by a CD38high; CX3CR1low; HLA-DRlow state within four days of hospital admittance. Together, our data reveal the chronic preservation of monocyte exhaustion, partially controlled by TICAM2.

Dispersion characteristics of slurry particles in a gas–solid fluidized bed with continuous slurry injection

Dispersion characteristics of slurry particles in a gas–solid fluidized bed with continuous slurry injection

Research on muck conditioning for EPB shield tunnelling in composite formation

Compared with simple formations, EPB (earth pressure balance) shield tunnelling in composite formations encounters severe problems with muck conditioning and require improved muck conditioning technology to fulfil expectations for continuous and efficient excavation. In the Nanchang Metro Line 4 Project, a water-rich sand-argillaceous siltstone composite formation is encountered. With a high moisture content and complex composite formation ratio, it is quite difficult to determine the optimum muck conditioning scheme, and thus, muck spewing accidents frequently occur during the tunnelling process. In this study, laboratory muck conditioning tests are conducted for five ratios of stratum, from a full section water-rich sand formation to a water-rich sand-argillaceous siltstone composite formation. The muck conditioner ratio for EPB shield tunnelling in such composite formations is dynamically optimized based on an analysis of propulsion speed, penetration rate, thrust force and torque of the shield machine in the field. The following muck conditioning scheme is obtained: (1) For the full-section water-rich sand layer, foam and bentonite slurry should have similar proportions, namely, the foam injection ratio is 10–15% and the bentonite slurry injection ratio is 10%. (2) As the argillaceous siltstone content increases from 10 to 90%, the foam injection rate gradually increases from 20 to 50%, and the bentonite slurry injection rate gradually decreases from 20% to 0. (3) If the argillaceous siltstone content is 10%, then CMC polymer with an injection ratio of 1% is required. Once the argillaceous siltstone content exceeds 70%, it is necessary to add mudstone dispersant solution (1:5) with a volume ratio of 2 to 3%. According to the analysis of the field tunnelling parameters, including penetration rate, total shield thrust and cutterhead torque, the optimization of the muck conditioning scheme proposed based on laboratory tests is proved effective and deserves further application.

Penetration Grouting Mechanism of Bingham Fluid in Porous Media Based on Fractal Theory

Penetration grouting is a significant grouting technique. The pore structure has important impacts on the infiltration mechanism of slurry in porous media. In this study, based on fractal theory, a theoretical penetration grouting model for Bingham fluid is established. An experimental apparatus for simulating the penetration process of Bingham fluid with a constant flow rate is developed. A series of penetration-grouting experiments are conducted to validate the theoretical model established in this study and analyze the impacts of the water–cement ratio and flow rate on the slurry injection pressure. The results show that the theoretical values of the slurry pressure along the penetration direction obtained from the penetration grouting model match the experimental values well. This indicates that the proposed model can better describe the process of slurry infiltration and provide valuable support for related grouting projects.

Identifying NH3 emission mitigation techniques from farm to field using a Bayesian network.

This study addresses the challenge of reducing ammonia (NH3) emissions from agriculture by evaluating various mitigation techniques. The research utilized a Bayesian Belief Network (BBN) to integrate quantitative data on NH3 volatilization reduction with qualitative stakeholder perceptions, aiming to identify the best available techniques (BATs) that balance environmental, economic, and socio-cultural factors for farmers in the Veneto region of Italy. The BBN framework established probabilistic dependencies between variables related to livestock, crop type, manure storage, fertilization management, and pedo-climatic conditions. Stakeholder opinions were quantified through a value elicitation process and combined with the BBN to create an integrated Influence Diagram (ID). Results indicated that effective NH3 reduction requires a comprehensive approach across the entire agri-livestock supply chain. Based on the results obtained, no single technique clearly emerged as the primary focus, rather various areas would require improvement across the agri-livestock supply chain. However, if prioritizing techniques were necessary, efforts should concentrate on stable management of infirmary animals (HCInf), overcrowding reduction by decreasing the number of animals on densely populated farms (OC-Animal), and optimization of protein in animal ration (FDProt). These measures should be combined with effective manure application through slurry injection (INJSlu) in the field. Stakeholders showed reluctance towards more expensive or innovative methods, indicating that socio-cultural perceptions and economic feasibility can heavily influence the adoption of new technologies although they proved to be among the most environmentally effective. The primary insight from applying the BBNs was that selecting effective techniques necessitates a multi-perspective approach to foster consensus among stakeholders throughout the agri-livestock supply chain.

Post-sepsis chronic muscle weakness can be prevented by pharmacological protection of mitochondria.

Sepsis, mainly caused by bacterial infections, is the leading cause of in-patient hospitalizations. After discharge, most sepsis survivors suffer from long-term medical complications, particularly chronic skeletal muscle weakness. To investigate this medical condition in detail, we previously developed a murine severe sepsis-survival model that exhibits long-term post-sepsis skeletal muscle weakness. While mitochondrial abnormalities were present in the skeletal muscle of the sepsis surviving mice, the relationship between abnormal mitochondria and muscle weakness remained unclear. Herein, we aimed to investigate whether mitochondrial abnormalities have a causal role in chronic post-sepsis muscle weakness and could thereby serve as a therapeutic target. Experimental polymicrobial abdominal sepsis was induced in 16-18months old male and female mice using cecal slurry injection with subsequent antibiotic and fluid resuscitation. To evaluate the pathological roles of mitochondrial abnormalities in post-sepsis skeletal muscle weakness, we utilized a transgenic mouse strain overexpressing the mitochondria-specific antioxidant enzyme manganese superoxide dismutase (MnSOD). Following sepsis development in C57BL/6 mice, we evaluated the effect of the mitochondria-targeting synthetic tetrapeptide SS-31 in protecting mitochondria from sepsis-induced damage and preventing skeletal muscle weakness development. In vivo and in vitro techniques were leveraged to assess muscle function at multiple timepoints throughout sepsis development and resolution. Histological and biochemical analyses including bulk mRNA sequencing were used to detect molecular changes in the muscle during and after sepsis RESULTS: Our time course study revealed that post sepsis skeletal muscle weakness develops progressively after the resolution of acute sepsis and in parallel with the accumulation of mitochondrial abnormalities and changes in the mitochondria-related gene expression profile. Transgenic mice overexpressing MnSOD were protected from mitochondrial abnormalities and muscle weakness following sepsis. Further, pharmacological protection of mitochondria utilizing SS-31 during sepsis effectively prevented the later development of muscle weakness. Our study revealed that the accumulation of mitochondrial abnormalities is the major cause of post-sepsis skeletal muscle weakness. Pharmacological protection of mitochondria during acute sepsis is a potential clinical treatment strategy to prevent post-sepsis muscle weakness.

The Petrogenesis of the Lower Critical Zone and Lower Group Chromitites, Eastern Bushveld Complex: Incremental Development of Igneous Layering and Injection of Chromite Slurries

Abstract The petrogenesis of the mafic-ultramafic layered rocks of the Bushveld Complex, South Africa, remains uncertain despite more than a century of intensive research. There is little evidence for the existence of a shallow crustal magma chamber, a principle entrenched in the scientific literature for many years, and we prefer an alternative hypothesis in which igneous layering developed from successive intrusive events. Geochemical discontinuities are explained by radical switches in the composition of intruding parental magmas. Here we investigate the origin of the Lower Critical zone, a sequence of five pyroxenitic and two peridotitic units containing the Lower Group (LG) of chromitites, of which LG6 is a major resource for chromium ore in the eastern limb. The pyroxenitic and peridotitic units are not part of magmatic cycles. Two discrete lineages of parental magma are recognized: a pyroxenitic magma sourced from deep crustal staging chambers, and a mantle-derived peridotitic magma. The pyroxenitic units reveal an absence of modal layering (with the exception of the 0.1- to 1-m-thick layers of chromitite) and limited height-related fractionation trends (enstatite: En86–811). Magma was fed as thin laminae. Intrusion rates were insufficient to create a magma reservoir because the supply of magma matched the rate of batch crystallization. There is little evidence of a fractionated residue having been removed. The recognition of minor geochemical trends of Mg- and Fe-enrichment in the pyroxenitic units reflects minor oscillations in the composition of the parental magma. The composition of the orthopyroxene and of the Cr spinel was primarily established at depth. The anomalous concentrations of chromite are explained by the introduction of pyroxenitic magmas containing crystals of Cr spinel, sufficiently abundant in some cases as to have created crystal slurries. The Cr spinel was entrained at depth, in the staging chambers. Each layer of chromitite is related to injection of a crystal slurry. The tiny size of the rounded xenocrysts of Cr spinel facilitated the mobile and dynamic nature of slurries. Coarse-grained mosaics of Cr spinel developed in the compacted layers of chromitite from prolonged periods of annealing. The irregular spacing of the chromitites, and occurrence of variable concentrations of disseminated Cr spinel in the pyroxenites, is explained by fluctuations in the proportion of entrained xenocrysts, rather than changes in the composition of the melt fraction of the magma. The peridotitic units are interpreted as sills, the chromitite layers having been inherited from the earlier formed pyroxenitic stratigraphy. The most primitive layers of dunite and harzburgite (olivine: Fo90-85 in which Fo = forsterite) accumulated in the center of sills, where a high magma flux facilitated growth of pegmatitic oikocrysts.

Effect of surface air cooling on the high frequent cyclic ablation of C/C-SiC-AlSi composite prepared by slurry injection technique

C/C-SiC-AlSi composites with uniform distribution of SiC ceramic phase were prepared by ultrasound-assisted slurry injection combined with the PIP+PI process. Ablation tests were performed by alternant plasma and surface cooling airflow for 0.2 s×200 cycles under the flame temperature of 2600 K. Results showed that the mass and linear ablation rates continued to decrease with the increasing of airflow rate from 0 to 3 m3/h, the minimum ablation rates were 0.1525 mg/s and 3.1725 μm/s, respectively. Micro-morphologies and surface temperature analysis suggested that ablation behavior of the composites was affected by the cooling effect of airflow and mechanical erosion. On the one hand, surface temperature of composites reduced and local physical and chemical reactions of the composites alleviated; on the other hand, the increase of airflow rate caused surface cracks and fragments of the composites. According to the ablation results, the former should play a leading role more than the latter.

Effects on upper masonry structures caused by double-line parallel shield cutting group pile construction

The effects on the upper masonry structure and the construction parameters of shield cutting piles were studied during shield construction, focusing on a shield interval of Zhengzhou Metro Line 5. The study utilized the actual engineering case of left and right double-lane shields superimposed on cutting cement soil group pile composite foundations beneath masonry structures. Findings revealed that masonry structures within approximately 30 m (5.0 times the tunnel diameter) were impacted before and after shield cut pile construction, resulting in deflection and twisting deformations of houses along the central axes of the left and right tunnel lines. Implementation of “clay shock” grouting outside the shield shell, radial grouting through small conduits, shield tail synchronous grouting, and secondary reinforcement grouting effectively mitigated the disturbance caused by shield construction to the ground. When shield cut piles passed beneath masonry structures, pressure on the soil chamber, total thrust, and cutterhead speed were consistently controlled. Furthermore, the cutterhead torque was appropriately reduced, and slurry injection volume increased, contributing to better control of house settlement.

The Effect of Fracture Wall Surface Roughness on Proppant Transport

Summary Proppant transport into created fractures is crucial in maximizing hydrocarbon recovery in unconventional reservoirs. Injected proppants keep the created fractures open, enhancing final fracture conductivity and propped and effective fracture length. The roughness and width of the created fracture are also important in proppant transport and distribution within induced fractures, affecting fracture conductivity. This study investigates the effect of fracture width on proppant transport into a complex slot system with 3D-printed rough wall surfaces. This study builds upon previous experimental work (Tatman et al. 2022), exploring different fracture roughness profiles and varying fracture widths. The effects of proppant densities, sizes, and concentrations on proppant transport within rough fracture surfaces were also investigated. A laboratory-scaled slot apparatus was used to examine the effects of various parameters. The slot consisted of a 4-ft-long primary fracture intersecting with a secondary fracture at a 90o angle. The wall surface roughness was printed using 3D printing technology and average fracture widths were set at 0.1 in. and 0.2 in. Fresh water (1 cp) with proppant concentrations of 1 ppg and 2 ppg and proppant sizes of 100-mesh sand [2.65 specific gravity (SG)], 40/70-mesh sand (2.65 SG), and 35/45-mesh ultralightweight (ULW) proppant (1.07 SG) were tested. The results show that fracture wall roughness impacts proppant transport behavior. The rough wall surface formed irregular proppant dune shapes and trapped some of the injected proppant at different locations within the slot, which is distinct from smooth wall surfaces. Decreasing the fracture width of a rough wall surface had a significant impact on proppant transport. The majority of the injected proppant was transported away from the injection point due to the increased slurry velocity. This led to improved proppant transport due to redirected flow direction and associated decreased proppant settling velocities, and a decrease in the proppant-dune-building rate near the inlet point, which carried more proppant deep into the secondary slot. Increasing the proppant concentration had a positive impact on proppant transport within both tested fracture widths by increasing the proppant-dune-buildup rate along the fracture slot and increasing the proppant-covered area inside fractures. The slurry injection rate had a great impact on low-density proppant transport. Decreasing the injection rate for lighter proppant (1.07 SG) helped to build a proppant dune near the injection point, increasing the proppant-covered area. Conversely, the higher injection rate carried the majority of the lighter proppant farther and out of the slots. Larger proppant sizes, (i.e., 40/70-mesh sand) resulted in a large proppant-covered area for both tested fracture widths, due to more particle-to-wall interaction, particle-to-particle interaction, and high density, which increased the settling velocity. However, injecting lighter and larger proppant sizes, such as 35/45-mesh ULW proppant, resulted in less settling and more particles suspended during injection due to lower density.

Diffusion mechanism of quick-setting slurry in water-rich fractured rock mass based on circle-outburst diffusion model

Grouting is the most commonly used methods in dealing with water inrush issue in mine and tunnel engineering. In order to better predict the grouting effect, research on slurry diffusion mechanism became a hotspot for scholars. At present, the mainstream theoretical model used to study the slurry diffusion mechanism are the circle diffusion model and the modified ones in plane plate fracture. However, these models cannot explain the “contradiction” between the rapid setting characteristics of quick-setting slurry and the continuous long-term injection of slurry in grouting engineering, which cannot accurately predict the grouting pressure or grouting diffusion range. Therefore, a “circle-outburst diffusion model” which can explain the above “contradiction” was proposed in this paper. Based on the new model, stepwise algorithms were developed to predict the grouting pressure and slurry diffusion range. By means of conducting fracture grouting simulation test and collecting grouting pressure data in an actual grouting project, the new model was verified and the slurry diffusion mechanism was studied. Comparison results indicate that the new model can reveal the intrinsic reason for the irregular diffusion phenomenon of the slurry and forecast the outburst moment accurately. The circle diffusion stage and outburst diffusion stage elaborated in the new “circle-outburst diffusion model” were consistent with the staged characteristics of the grouting process presented in the grouting simulating test. Differences between theoretical and experimental pressure value were within 10 %, indicating a high degree of consistency. The number and distribution of outburst diffusion points determine slurry diffusion form and significantly influence the diffusion range. The grouting pressure and the length of slurry flow path increase nonlinearly with time in each diffusion stage. It is hoped that the new model can provide a theoretical basis for the study of diffusion mechanism of quick-setting slurry.

Experimental study on the workability of sands conditioned with bentonite-silty clay modified slurry

With the widespread application of Earth Pressure Balance (EPB) shield technology, the generation of shield muck has been increasing yearly. This paper aims to investigate the effectiveness of bentonite-silty clay modified slurry (BSC) as a soil conditioner for enhancing the workability of sands during EPB shield tunneling, thus enabling the recycling and reuse of the discarded muck (waste silty clay). Standard slump tests were conducted on three typical sand specimens from Shenyang Metro Line 6. The influence of the types of conditioners and slurry injection ratio (SIR) on slump values were examined to determine an optimal conditioning scheme tailored to the specific formation conditions. Furthermore, the study explored the combined use of BSC and foam to improve workability, employing a three-factor four-level orthogonal experiment. Finally, the rheological parameters (yield stress) derived from the slump tests provide valuable insights for assessing material flow within the tunneling system. The results show that comparative analyses with pure bentonite slurries reveal that BSC is a suitable, economical, and effective alternative for soil conditioning. The particle size distribution of sand specimens significantly influences the conditioning process, necessitating adjustments to SIR and slurry viscosity for optimal results. When the slump value of slurry-conditioned soil falls within the range of 150–250 mm, the slump test can be effectively used to estimate its yield stress under atmospheric conditions. This study contributes to the development of sustainable and economical solutions for soil conditioning in urban tunnel projects, particularly by utilizing excavated materials effectively.

Multi-scale numerical analysis of Ca-based desulfurizer dynamics characteristics and SO2 absorption processes in industrial-scale SDA-FGD reactor

Spray dry absorption flue gas desulfurization technology is widely employed to control sulfur dioxide emissions from industrial pollutant gases. This study employs the multiphase particle-in-cell method to numerically simulate gas–solid heat transfer, SO2 removal processes, and the effect of operating conditions in the SDA reactor. The flue gas desulfurization model used has been experimentally validated. The results indicate that flue gas and slurry droplets exhibit swirling flow along the reactor walls, forming gas vortices at the top and near the outlet. The temperature and Reynolds number of slurry droplets show minimal variation when particle size is below 43 μm. The heat transfer coefficient of slurry droplets is notably higher at the reactor top and increases with the slip velocity of the droplets. Furthermore, the CaSO3 volume fraction increases with longer residence times of the slurry droplets in the reactor. When the height and diameter of the main reaction area are lower than 14 m and 8.5 m, respectively, the SO2 mass fraction in the central area undergoes significant changes. The SO2 inlet concentration is negatively correlated with desulfurization efficiency, whereas the slurry injection speed, along with the diameter and height of the main reaction region, are positively correlated with desulfurization efficiency. Among these factors, the SO2 inlet concentration and the height of the main reaction region have the most substantial impact on desulfurization efficiency.

A DTSD model based on PS-InSAR surface multi-type deformation monitoring of villages in the mining area

Evaluating building damage in mining areas using Interferometric Synthetic Aperture Radar (InSAR) has inherent challenges. This work presents a Delaunay Triangulation of Surface Deformation (DTSD) model that utilizes Permanent Scatter InSAR to analyze various types of deformations in villages. This model created a network of irregular triangles using Delaunay triangulation and PS points as the input. The calculation involved determining the inclination of triangular edges and the torsion of spatial Bezier curves fitted by these edges. The PS points' maximum inclination and curvature values were identified by solving over-determined equations. Results from a slurry injection mine in Huaibei, China, indicate that the various types of deformations in the neighboring communities were below 8 %, and the buildings experienced minimal damage. The DTSD model offers a theoretical framework for monitoring and assessing various types of surface deformation and designing essential zones for building protection.

Improving Human Diets and Welfare through Using Herbivore-Based Foods: 2. Environmental Consequences and Mitigations.

Animal-sourced foods are important for human nutrition and health, but they can have a negative impact on the environment. These impacts can result in land use tensions associated with population growth and the loss of native forests and wetlands during agricultural expansion. Increased greenhouse gas emissions, and high water use but poor water quality outcomes can also be associated. Life cycle analysis from cradle-to-distribution has shown that novel plant-based meat alternatives can have an environmental footprint lower than that of beef finished in feedlots, but higher than for beef raised on well-managed grazed pastures. However, several technologies and practices can be used to mitigate impacts. These include ensuring that grazing occurs when feed quality is high, the use of dietary additives, breeding of animals with higher growth rates and increased fecundity, rumen microbial manipulations through the use of vaccines, soil management to reduce nitrous oxide emission, management systems to improve carbon sequestration, improved nutrient use efficacy throughout the food chain, incorporating maize silage along with grasslands, use of cover crops, low-emission composting barns, covered manure storages, and direct injection of animal slurry into soil. The technologies and systems that help mitigate or actually provide solutions to the environmental impact are under constant refinement to enable ever-more efficient production systems to allow for the provision of animal-sourced foods to an ever-increasing population.

Short-term effect of liquid organic fertilisation and application methods on N2, N2O and CO2 fluxes from a silt loam arable soil

AbstractThe absence of N2 flux measurements in liquid manure-amended soils has resulted in a poor understanding of the effect of manure application on gaseous N losses. The aim of this study was to quantify N2, N2O, CO2, N2O reduction to N2, depth distribution of moisture, water-extractable organic C, NO3−, NH4+, pH, and diffusivity in a laboratory incubation experiment with an arable silt-loam soil. To quantify N processes and gaseous fluxes, 15N tracing was applied. An artificial livestock slurry-mixture was added to the soil in various treatments (control, surface or injected application; slurry-application rate: 42.9 kg N ha− 1; soil water content of either 40% or 60% water-filled pore space (WFPS)). The soil was incubated for 10 days. The depth distribution of the control parameters was measured twice during the experiment on days 5 and 10. The average increase in N2 and N2O fluxes from denitrification was about 900% in slurry-amended soils. The highest N2 and N2O fluxes from denitrification were measured in the slurry injection, 60% WFPS treatment (7.83 ± 3.50 and 11.22 ± 7.60 mg N m− 2 d− 1, respectively). The hypothesis that injected slurry at a higher water content enhances denitrification was confirmed. This study provides important insights into the formation, spatial and temporal variation of the manure-soil hotspot and its impact on the denitrification process. The results will form part of a dataset to develop, improve and test manure application submodules of biogeochemical models and will help to understand in detail the effect of hotspots on N-cycling in manure-treated soils.

Gangue grouting filling in subsequent space of coal green mining: methodology and case study

Underground backfilling stands out as a crucial technological strategy for the eco-friendly and effective management of solid waste in mining operations. However, existing backfilling techniques have led to increased production processes at the working face, resulting in a reduction in coal extraction efficiency. Addressing the temporal and spatial interference between mine solid waste backfilling and coal mining is essential. To overcome this challenge, this study introduces a novel post-mining spatial gangue slurry backfilling method. Radar detection was employed to ascertain the typical characteristics of the subsequent space collapse roof shape. Stress monitoring and compaction experiments were conducted to establish the relationship between stress and the bulking coefficient of the overlying rock mass, identifying subsequent spatial void structure characteristics. The development of a CO2 mineralized coal-based solid waste filling material, utilizing conventional low-calcium fly ash under normal temperature and pressure conditions, was presented. This paper provides a comprehensive understanding of the post-mining spatial gangue slurry backfilling method, outlines the spatial layout approach for the corresponding system, and analyzes research challenges associated with gangue slurry backfilling materials and the technology of slurry injection borehole layout. The research aims to innovate an efficient underground disposal model for gangue, contributing to the refinement of the technical system for the comprehensive disposal and utilization of gangue.

Slurry acidification is as effective as slurry injection at reducing ammonia emissions without increasing N2O emissions: A short-term mesocosm study

Agriculture currently accounts for 90% of UK ammonia (NH3) emissions, however, national legally-binding targets are seeking to reduce this by 16% by 2030, relative to 2005 levels. One area which has been targeted to help achieve this target is the lowering of NH3 emissions during livestock manure handling and storage. Slurry acidification represents one potential strategy to abate NH3 throughout the slurry management chain, however, is not currently used in the UK. To address this knowledge gap, two mesocosm-scale experiments were set up to assess the potential for slurry acidification to reduce NH3 emissions at application and to measure impacts on NH3 emissions and short-term changes to soil mineral dynamics and N2O emissions. Experiment 1 determined the impacts of acidified cattle slurry (pH 6.5, 5.5, and 4.8) to conventional (non-acidified) slurry when simulating surface broadcasting. Experiment 2 assessed the impact of conventional and acidified slurry (pH 5.5) using simulated surface broadcasting and shallow injection of cattle slurry. Our results showed that acidification significantly abated NH3 (% of NH4-N applied) from 61.6% for conventional slurry to 26.6% at pH 5.5, and 2.5% at pH 4.8. Acidified surface broadcast was as effective at abating NH3 emissions to injected conventional slurry, and also delayed nitrification, while not significantly altering N2O emissions from conventional slurry. Our results strongly suggest that slurry acidification could be a viable strategy to help the UK reach its NH3 reduction target and exceed the current abatement potential through combining low emissions spreading techniques with acidification.

An improved mouse model of sepsis based on intraperitoneal injections of the enriched culture of cecum slurry

AimSepsis is a life-threatening clinical syndrome comprising multiorgan dysfunctions caused by a disproportionate body immune response. There are several animal sepsis models which are based on cecum ligation, cecal puncture, and cecum slurry injection. The major limitation of all current sepsis models is the high variability owing to the variable degree of ligation, puncture and inconsistent microbial composition used for sepsis initiation. The primary objective of this work is to demonstrate the feasibility of a standardized method for sepsis development. Materials and methodsThe cecal slurry bacterial culture was developed and preserved in glycerol stocks. Antibiotics aztreonam and vancomycin were used for generating several defined, enriched cecal slurry bacterial cultures. Mice survival was assessed until 48 hrs post injection, and the tissue samples were collected after 10 hrs from sepsis initiation. Key findingsThe results indicate that increasing polymicrobial load resulted in lower survival rates and was associated with the higher number of infiltrating immune cells and necrosis. H&E (haematoxylin & eosin) staining & serum markers revealed that septic mice exhibited increased inflammation and significant damage to the liver and kidneys. The defined Gram-negative and Gram-positive specific cecal slurry bacterial cultures were developed and their efficiency in inducing sepsis was characterized. SignificanceEnriched cecal slurry bacterial cultures can be stored in glycerol stocks at −80 °C. This has an ethical advantage of avoiding unnecessary animal euthanasia for each experiment and provides a standardization capability of sepsis development.

Rheological characterization of the conditioned sandy soil under gas-loading pressure for earth pressure balance shield tunnelling

Soil conditioning technology is usually required to modify the excavated soil to a fluid plastic state during the construction with earth pressure balance (EPB) shield. The steady pressure distribution in the excavation face is linked to soil fluidity. Compared with the slump test, the rheological behavior of the conditioned soil can better reflect the dynamic flow characteristics. A gas-loading rotational rheometer is developed to test the rheological properties of the conditioning agents and the conditioned sandy soil, which can overcome the disadvantage of uneven mechanical loading and create gas-loading conditions. The rheological properties of sandy soil conditioned by different agents under atmospheric and gas-loading pressure conditions were studied, and the influences of foam injection ratio (FIR), bentonite slurry injection ratio (SIR), and polymer injection ratio (PIR) on soil viscosity were analyzed. The test results show that the ambient air pressure only greatly influences the experimental group with foam. Under the same gas-loading pressure, the foam’s apparent viscosity decreases with the foam expansion ratio (FER) increasing. The rheological behavior of the conditioned sandy soil conforms to the Bingham model under atmospheric pressure and conforms to the Power Law model when PIR ≤ 5 % under gas-loading pressure of 3 bar. The Weissenberg effect accounts for the differences. When PIR > 10 %, the rheological curve of three agents conditioned sand conforms to the Herschel Bulkley model. The higher content polymer reacts with bentonite to increase the soil viscosity, and blocks the foam seepage channel, making it difficult for the foam to re-enter the soil under gas-loading pressure. Investigating the rheological behavior of different conditioned sandy soil provides optimization strategies for EPB performance.