Injection Of Sheep Erythrocytes Research Articles

The Sustained Suppression of Hemolysin Response in Passively Immunized Rats

Summary When adult rats were immunized with sheep erythrocytes, the secondary hemolysin response was lower than the primary response. The effect of circulating hemolysin on the rat's response to sheep erythrocytes was investigated. A marked inhibition of the primary hemolysin response in the rat was produced by administering homologous anti-sheep erythrocyte serum 24 hr before the injection of sheep erythrocytes. Significant inhibition of the antibody response occurred with a wide range of doses of antiserum and antigen. Inhibition of the antibody response by antiserum occurred when rats were actively immunized intravenously, intraperitoneally, or intradermally. The presence of circulating hemolysin was necessary to demonstrate the inhibition of the hemolysin response. Unresponsiveness to antigenic stimulation induced by a single passive immunization was sustained if small doses of sheep erythrocytes were injected at weekly intervals. The possible mechanisms responsible for this phenomenon were discussed.

Antibody response in rabbits following injection of sheep erythrocytes into lateral ventricle of brain.

IMMUNOLOGICAL studies of the cerebrospinal fluid have been confined mainly to the passage of antibodies from plasma into the cerebrospinal fluid1. However, the investigation of exchange between cerebrospinal fluid and blood has revealed that various electrolytes2, dyes3 and proteins4 may find their way from cerebrospinal fluid into general circulation. Furthermore, some data5 indicate that red blood cells cross cerebrospinal fluid-blood barrier. It seemed, therefore, advisable to approach the immunological sequences following the injection of heterologous erythrocytes into lateral ventricle of the brain.

Effect of X Rays upon Hemolysin Production in the Rat

Summary Rats were irradiated once with X rays (250 kvp, LD50 of 725 r) at various times during the week before and the 5 days after an injection of sheep erythrocytes, and the hemolysin titer followed. Normally, the peak titer occurred 5 to 6 days after antigen injection. Following irradiation, three principal effects were noted. The peak titer was reduced. The attainment of the peak was delayed. The decline in titer after the peak was delayed. No evidence was obtained that irradiation released appreciable and lasting amounts of hemolysin, or accelerated its disappearance. Exposure at 1 to 3 days before antigen injection was the most effective per unit dose, 175 r causing marked inhibition. Exposure within several hours of antigen injection was less effective, and the results tended to be more variable than in other experiments. Exposure at 1 or 2 days after antigen injection required 350 r to produce an inhibition. Although delay in reaching the peak titer was marked, reduction in the peak was not great. As a result, the titers of the irradiated animals were below the controls during the first 10 days, equal to them during days 10 to 14, and above them thereafter. Exposure to 350 to 600 r on the second to fourth days after antigen injection, when measurable titers were about to appear or had appeared in the plasma, led to inhibition within 24 hours.